1. Membrane Transporter/Ion Channel
  2. Calcium Channel

Ionomycin 

Cat. No.: HY-13434 Purity: >98.0%
Handling Instructions

Ionomycin is a Calcium ionophore and an antibiotic produced by Streptomyces conglobatus ATCC 31005.

For research use only. We do not sell to patients.

Ionomycin Chemical Structure

Ionomycin Chemical Structure

CAS No. : 56092-81-0

Size Price Stock Quantity
14.1 mM*100  μL in Ethanol USD 110 In-stock
Estimated Time of Arrival: December 31
14.1 mM*500  μL in Ethanol USD 390 In-stock
Estimated Time of Arrival: December 31
1 mg   Get quote  
5 mg   Get quote  

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  • Biological Activity

  • Technical Information

  • Purity & Documentation

  • References

Description

Ionomycin is a Calcium ionophore and an antibiotic produced by Streptomyces conglobatus ATCC 31005.

IC50 & Target

Calcium channel[1].

In Vitro

Ionomycin is a Calcium ionophore and an antibiotic produced by Streptomyces conglobatus ATCC 31005[1]. It is observed that LCLC 103H cells overexpressing the catalytic subunit of μ-calpain rapidly underwent cell death after treatment with ionomycin. The first signs of Ionomycin-induced cell death are detected 3 h after addition of the ionophore. Addition of 2 μM Ionomycin to LCLC 103H cells causes an instantaneous increase in intracellular Ca2+ concentration from 50 to 180 nM. Remarkably, calcium concentrations are raised transiently to 0.8-1.5 μM. DNA and protein analysis in Ionomycin-treated cultures revealed DNA fragmentation and PARP cleavage to an 85-kDa fragment typical of caspase-mediated apoptosis. Three hours after addition of ionomycin, the percentage of early apoptotic cells approximately doubles. Necrosis could be detected in ~1-5% of the Ionomycin treated cells as supported by simultaneously positive fluorescein labeling and propidium iodide uptake. Caspase activation in whole cells was followed by monitoring the increase in activity against Ac-DEVD-amc following Ionomycin treatment[2].

Clinical Trial
Solvent & Solubility
In Vitro: 

Ethanol : ≥ 10 mg/mL (14.10 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4104 mL 7.0521 mL 14.1042 mL
5 mM 0.2821 mL 1.4104 mL 2.8208 mL
10 mM 0.1410 mL 0.7052 mL 1.4104 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Molecular Weight

709.01

Formula

C₄₁H₇₂O₉

CAS No.

56092-81-0

SMILES

O=C(O)CC[[email protected]@H](C)C[[email protected]](C)C[[email protected]](C)C(/C=C(O)/[[email protected]](C)C[[email protected]](C)C/C=C/[[email protected]@H](C)[[email protected]@H](O)[[email protected]@H](C)[[email protected]@H](O)C[[email protected]@H]1CC[[email protected]]([[email protected]@H]2O[[email protected]](C)([[email protected]](O)C)CC2)(C)O1)=O

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Purity: >98.0%

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Product Name:
Ionomycin
Cat. No.:
HY-13434
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Ionomycin

Cat. No.: HY-13434