2. Metabolic Enzyme/Protease Anti-infection Apoptosis Autophagy
  3. Oxidative Phosphorylation Bacterial Fungal Apoptosis Autophagy Antibiotic
  4. Calcimycin

Calcimycin  (Synonyms: A-23187; Antibiotic A-23187)

Cat. No.: HY-N6687 Purity: 99.10%
COA
SDS
Handling Instructions Technical Support

Calcimycin (A-23187) is an antibiotic and a unique divalent cation ionophore (like calcium and magnesium). Calcimycin induces Ca2+-dependent cell death by increasing intracellular calcium concentration. Calcimycin inhibits the growth of Gram-positive bacteria and some fungi. Calcimycin also inhibits the activity of ATPase and uncouples oxidative phosphorylation (OXPHOS) of mammalian cells. Calcimycin induces apoptosis and autophagy.

For research use only. We do not sell to patients.

CAS No. : 52665-69-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 mg In-stock
5 mg In-stock
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Customer Review

Based on 24 publication(s) in Google Scholar

Other Forms of Calcimycin:

Calcimycin Related Antibodies

Top Publications Citing Use of Products

    Calcimycin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 20:e11873.  [Abstract]

    A23187 (5 µM; 1–6 h) induced apoptosis in microglia in vitro. Microglia viability was assessed using the CCK-8 assay.

    Calcimycin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 20:e11873.  [Abstract]

    A23187 (5 µM; 4 h). Representative images of microglia stained with Iba1, citH3, and MPO triple staining, and colocalization analysis of MPO, citH3, and DNA in the three cell groups.

    Calcimycin purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2023 Jan;43(1):123-149.  [Abstract]

    Cells were treated with different concentrations of Calcimycin (A23187: 2 µmol/L, 4 µmol/L, 8 µmol/L, 10 µmol/L; 24 h), and the effect of A23187 on STAT1 expression was assessed by Western blotting.

    Calcimycin purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2023 Jan;43(1):123-149.  [Abstract]

    Cells were treated with A23187 (4 µmol/L; 24 h) to examine the effect of A21187 on the binding ability of TRIM21 and STAT1 by Co‐IP and western blotting, and the result showed that A23187 reversed the dissociation of TRIM21 and STAT1 in the context of TGM2 and increased ubiquitination of STAT1.

    Calcimycin purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2023 Jan;43(1):123-149.  [Abstract]

    The effect of A23187 (4 µmol/L; 0-96 h) and ZM39923 (20 nmol/L) on GC cell proliferation was evaluated by CCK8 assay. The result showed that ZM39923 promoted cell proliferation while A23187 had an inhibitory role in GC cell proliferation.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Calcimycin (A-23187) is an antibiotic and a unique divalent cation ionophore (like calcium and magnesium). Calcimycin induces Ca2+-dependent cell death by increasing intracellular calcium concentration. Calcimycin inhibits the growth of Gram-positive bacteria and some fungi. Calcimycin also inhibits the activity of ATPase and uncouples oxidative phosphorylation (OXPHOS) of mammalian cells. Calcimycin induces apoptosis and autophagy[1][2][3][4][5].

    In Vitro

    Calcimycin (A-23187) mediates mycobacterial killing by inducing intracellular calcium-regulated autophagy in a P2RX7 dependent manner[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Calcimycin Related Antibodies
    In Vivo

    Calcimycin (A-23187) (2.5 or 7.5 nM; intrapleurally) induces protein leakage[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Mice (ICR, 25-30 g)[5]
    Dosage: 2.5 or 7.5 nM
    Administration: Intrapleurally
    Result: Two hours after 2.5 nM, or three hours after 7.5 nM, challenge the protein levels in the pleural cavity were equivalent to about a half of their corresponding peak values.
    Clinical Trial
    Molecular Weight

    523.62

    Formula

    C29H37N3O6
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(C1=C2N=C(C[C@H]3O[C@]4(O[C@H]([C@H](C)C(C5=CC=CN5)=O)[C@H](C)C[C@H]4C)CC[C@H]3C)OC2=CC=C1NC)O
    Structure Classification
    Initial Source

    Streptomyces chartreusensis
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 33.33 mg/mL (63.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9098 mL 9.5489 mL 19.0978 mL
    5 mM 0.3820 mL 1.9098 mL 3.8196 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (4.77 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (4.77 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.12%

    SDS (396 KB)

    COA (252 KB) HNMR (297 KB) LCMS (263 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.9098 mL 9.5489 mL 19.0978 mL 47.7445 mL
    5 mM 0.3820 mL 1.9098 mL 3.8196 mL 9.5489 mL
    10 mM 0.1910 mL 0.9549 mL 1.9098 mL 4.7745 mL
    15 mM 0.1273 mL 0.6366 mL 1.2732 mL 3.1830 mL
    20 mM 0.0955 mL 0.4774 mL 0.9549 mL 2.3872 mL
    25 mM 0.0764 mL 0.3820 mL 0.7639 mL 1.9098 mL
    30 mM 0.0637 mL 0.3183 mL 0.6366 mL 1.5915 mL
    40 mM 0.0477 mL 0.2387 mL 0.4774 mL 1.1936 mL
    50 mM 0.0382 mL 0.1910 mL 0.3820 mL 0.9549 mL
    60 mM 0.0318 mL 0.1591 mL 0.3183 mL 0.7957 mL
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    Calcimycin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Calcimycin52665-69-7A-23187 Antibiotic A-23187A23187A 23187Antibiotic A23187Antibiotic A 23187Antibiotic A-23187Oxidative PhosphorylationBacterialFungalApoptosisAutophagyAntibioticmycobacterialdivalentcationionophoreGram-positivebacteriaphosphorylationInhibitorinhibitorinhibit

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