1. Induced Disease Models Products Metabolic Enzyme/Protease
  2. Immunology and Inflammatory Disease Models Endogenous Metabolite
  3. Paw Edema Models
  4. Arachidonic acid

Arachidonic acid  (Synonyms: Immunocytophyt)

Cat. No.: HY-109590 Purity: 99.32%
COA Handling Instructions

Arachidonic acid (Immunocytophyt) is a polyunsaturated omega-6 fatty acid and a major constituent of biomembranes. Arachidonic acid also acts as the substrate for various lipid mediators, such as prostaglandins (PGs). Arachidonic acid improves cognitive response and cardiovascular function.

For research use only. We do not sell to patients.

Arachidonic acid Chemical Structure

Arachidonic acid Chemical Structure

CAS No. : 506-32-1

Size Price Stock Quantity
Liquid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Liquid
5 mg USD 50 In-stock
10 mg USD 70 In-stock
50 mg USD 125 In-stock
100 mg USD 150 In-stock
200 mg   Get quote  
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Customer Review

Based on 16 publication(s) in Google Scholar

Other Forms of Arachidonic acid:

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

Arachidonic acid (Immunocytophyt) is a polyunsaturated omega-6 fatty acid and a major constituent of biomembranes. Arachidonic acid also acts as the substrate for various lipid mediators, such as prostaglandins (PGs). Arachidonic acid improves cognitive response and cardiovascular function[1].

IC50 & Target

Human Endogenous Metabolite

 

In Vivo

Arachidonic acid can be used in animal modeling to construct a rat paw edema model. Arachidonic acid (0.07%, 0.15% or 0.32% in diet; 4 weeks) increases Arachidonic acid content in the paw, but has no effect on arthritis severity and PGE2 content of the paw in a rat arthritis model[1].
Induction of Paw Edema Model[2]
Background
Principle: Injecting arachidonic acid into the hind paws of rats can induce rapid and sustained inflammatory responses.
Specific Mmodeling Methods
Rats: Lewis • male •
Administration: 0.5% • s.c. • single dose
Note
Injection method: A single subcutaneous injection of Arachidonic acid in the right hind paw of male Lewis rats (144-241 g) with an injection volume of 0.10 mL (Arachidonic acid is dissolved in 0.2 M carbonate buffer, pH 8.43-8.56 ).


Modeling Indicators
Appearance Monitoring: Significant edema became apparent within 5 minutes, and the reaction reached its peak at 1 hour after injection.
Opposite Product(s): Phenidone (HY-W010144); SK-F86002 (HY-12511)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Lew rats (4-week-old) induced arthritis[1]
Dosage: 0.07%, 0.15% or 0.32% in diet (w/w)
Administration: 4 weeks
Result: The Arachidonic acid content of phospholipids in the paw was significantly elevated in a dose-dependent manner.
Clinical Trial
Molecular Weight

304.47

Formula

C20H32O2

CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(O)=O

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

Ethanol : 100 mg/mL (328.44 mM; Need ultrasonic)

DMSO : 100 mg/mL (328.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2844 mL 16.4220 mL 32.8440 mL
5 mM 0.6569 mL 3.2844 mL 6.5688 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.21 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (8.21 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 10 mg/mL (32.84 mM); Suspended solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.32%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 3.2844 mL 16.4220 mL 32.8440 mL 82.1099 mL
5 mM 0.6569 mL 3.2844 mL 6.5688 mL 16.4220 mL
10 mM 0.3284 mL 1.6422 mL 3.2844 mL 8.2110 mL
15 mM 0.2190 mL 1.0948 mL 2.1896 mL 5.4740 mL
20 mM 0.1642 mL 0.8211 mL 1.6422 mL 4.1055 mL
25 mM 0.1314 mL 0.6569 mL 1.3138 mL 3.2844 mL
30 mM 0.1095 mL 0.5474 mL 1.0948 mL 2.7370 mL
40 mM 0.0821 mL 0.4105 mL 0.8211 mL 2.0527 mL
50 mM 0.0657 mL 0.3284 mL 0.6569 mL 1.6422 mL
60 mM 0.0547 mL 0.2737 mL 0.5474 mL 1.3685 mL
80 mM 0.0411 mL 0.2053 mL 0.4105 mL 1.0264 mL
100 mM 0.0328 mL 0.1642 mL 0.3284 mL 0.8211 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Arachidonic acid
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