Arachidonic acid (Synonyms: Immunocytophyt)

Cat. No.: HY-109590: Data Sheet
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Arachidonic acid (Immunocytophyt) is a polyunsaturated omega-6 fatty acid and a major constituent of biomembranes. Arachidonic acid also acts as the substrate for various lipid mediators, such as prostaglandins (PGs). Arachidonic acid improves cognitive response and cardiovascular function.

For research use only. We do not sell to patients.

CAS No. : 506-32-1

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Other In-stock Forms of Arachidonic acid:

Arachidonic acid sodium salt

Other Forms of Arachidonic acid:

55 Publications Citing Use of MCE Arachidonic acid

Cell Imaging/Staining

Others

ELISA

Cell Proliferation/Viability Assay

: Arachidonic acid purchased from MedChemExpress. Usage Cited in: Science. 2025 Mar 14;387(6739):eadm9805. [Abstract]; HCC1806 cells were serum starved for 16 hours and subsequently restimulated for 30 minutes with 1μM, 5μM, 10μM, 30μM, 100μM, 200μM, 1000μM of ω-6 LA, ω-6 ARA, ω-3 ALA, ω-3 EPA and ω-3 DHA for 30 minutes. Intracellular concentrations of each PUFA were quantified by ELISA from lysates obtained from 5 x 10⁶ cells.

: Arachidonic acid purchased from MedChemExpress. Usage Cited in: Science. 2025 Mar 14;387(6739):eadm9805. [Abstract]; lmmunoblot analysis of HCC1806 cells following 16 hour serum starvation and subsequent stimulation with 10% FBS or 30 μM ω-6 LA or Arachidonic acid (AA), or ω-3 ALA, Eicosapentaenoic acid (EPA) or Docosahexaenoic acid (DHA) for 30 minutes.

: Arachidonic acid purchased from MedChemExpress. Usage Cited in: Nat Cancer. 2025 May;6(5):768-785. [Abstract]; The cell viability of HT-1080 cells treated with IFNγ (20 ng/mL) plus Arachidonic acid (AA, 20 μM) in conjunction with or without 2-BP (10 μM) and Fer-1 (2 μM) for 48 h.

: Arachidonic acid purchased from MedChemExpress. Usage Cited in: Ann Rheum Dis. 2025 Oct 28:S0003-4967(25)04428-0. [Abstract]; ELISA analysis of PGE2 concentrations in the supernatants of wild-type (WT) and TBXAS1-knockout (KO) U937 cell lines treated with indicated doses of Arachidonic acid (0-25 μM).

: Arachidonic acid purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Sep 29;16(1):8541. [Abstract]; BODIPY (green) and nuclei (red) staining of OVCAR8 cells treated with Arachidonic acid (70 μM) and embedded in 70 µm OHG for 7 d. Arrowheads indicate collectively-invading cells.

: Arachidonic acid purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jun 5;16(1):5209. [Abstract]; Representative images of live HeLa-Lyso-mKeima cells treated with DMSO or 100 μM Arachidonic acid for 15 min (left) and corresponding mKeima FLIs (right).

: Arachidonic acid purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Feb 3:80:103528. [Abstract]; OVCAR8 and A2780 cells were cultured in fatty acid-free media supplemented with 50 μM AA (arachidonic acid), 50 μM OA (oleic acid), 50 μM EPA (eicosapentaenoic acid), 50 μM PA (Palmitic acid), and 50 μM MA (myristic acid) with the treatment of 10 μM niraparib for 72 h, and the cell viability was determined.

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Human Endogenous Metabolite Microbial Metabolite

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
MDA-MB-468	EC₅₀	> 100 μM Compound: AA	Proapoptotic activity in human MDA-MB-468 cells assessed as activation of caspase-3 by luminometry Proapoptotic activity in human MDA-MB-468 cells assessed as activation of caspase-3 by luminometry	[PMID: 22822908]
MDA-MB-468	IC₅₀	> 100 μM Compound: AA	Antiproliferative activity against human MDA-MB-468 cells after 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-468 cells after 48 hrs by MTT assay	[PMID: 22822908]
RBL-2H3	IC₅₀	> 50 μM Compound: 5	Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity preincubated for 30 mins followed by DNP-HSA stimulation and measured after 30 mins by 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside substrate based assay Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity preincubated for 30 mins followed by DNP-HSA stimulation and measured after 30 mins by 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside substrate based assay	[PMID: 31618024]
THP-1	IC₅₀	180 nM Compound: AA-861	Concentration required to inhibit production of PGE-2 from THP-1 cells stimulated with A-23187 Concentration required to inhibit production of PGE-2 from THP-1 cells stimulated with A-23187	[PMID: 11229777]
THP-1	IC₅₀	4.7 nM Compound: AA-861	Concentration required to inhibit production of LTC4 from THP-1 cells stimulated with A-23187 Concentration required to inhibit production of LTC4 from THP-1 cells stimulated with A-23187	[PMID: 11229777]
THP-1	IC₅₀	40000 nM Compound: AA-861	Concentration required to inhibit production of arachidonic acid from THP-1 cells stimulated with A-23187 Concentration required to inhibit production of arachidonic acid from THP-1 cells stimulated with A-23187	[PMID: 11229777]
Ventricular myocyte	IC₅₀	5.8 μM Compound: Arachidonic acid	Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes	[PMID: 22761000]
Ventricular myocyte	IC₅₀	8.5 μM Compound: Arachidonic acid	Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes	[PMID: 22761000]

In Vitro

Arachidonic acid (0-100 µM, 24-72 h) decreases the viability of HL-60 cells in time- and dose-dependent manner^[3].
Arachidonic acid (0-100 µM, 6-24 h) reduces the G0-G1, S and G2-M DNA with increasing concentration and exposure time in HL-60 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Arachidonic acid can be used to induce paw edema models^[2].

Induction of Paw Edema Model^[2]

Background

Principle: Injecting arachidonic acid into the hind paws of rats can induce rapid and sustained inflammatory responses.

Specific Modeling Methods

Rat: Lewis • male •
Administration: 0.5% • s.c. • single dose

Note

A single subcutaneous injection of 0.10 mL of arachidonic acid (dissolved in 0.2 M carbonate buffer with a pH value of 8.43-8.56) was administered into the right hind paw of male Lewis rats weighing 144-241 grams.

Modeling Indicators

Appearance Monitoring: Significant edema became apparent within 5 minutes, and the reaction reached its peak at 1 hour after injection.

Opposite Product(s): phenidone (HY-W010144); SK&F86002 (HY-12511)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Lew rats (4-week-old) induced arthritis^[1]
Dosage:	0.07%, 0.15% or 0.32% in diet (w/w)
Administration:	4 weeks
Result:	The Arachidonic acid content of phospholipids in the paw was significantly elevated in a dose-dependent manner.

Clinical Trial

Molecular Weight

304.47

Formula

C₂₀H₃₂O₂

CAS No.

506-32-1

Appearance

Liquid (Density: 0.922 g/cm³)

Color

Colorless to light yellow

SMILES

CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(O)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Animals

mammalian cell

Shipping

Shipping with dry ice.

Storage

-80°C, protect from light, stored under nitrogen

Solvent & Solubility

In Vitro:

Ethanol : 100 mg/mL (328.44 mM; Need ultrasonic)

DMSO : 100 mg/mL (328.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2844 mL	16.4220 mL	32.8440 mL
5 mM	0.6569 mL	3.2844 mL	6.5688 mL
10 mM	0.3284 mL	1.6422 mL	3.2844 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (8.21 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.75%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (237 KB) RP-HPLC (215 KB)

Handling Instructions (2659 KB)

References

[1]. Tateishi N, et al. Dietary supplementation with arachidonic acid increases arachidonic acid content in paw, but does not affect arthritis severity or prostaglandin E2 content in rat adjuvant-induced arthritis model. Lipids Health Dis. 2015 Jan 16;14:3. [Content Brief]

[2]. DiMartino MJ, et al. The pharmacology of arachidonic acid-induced rat paw edema. Agents Actions. 1987 Aug;21(3-4):303-5. [Content Brief]

[3]. Pompeia C, et al. Arachidonic acid cytotoxicity in leukocytes: implications of oxidative stress and eicosanoid synthesis. Biol Cell. 2002 Sep;94(4-5):251-65. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	3.2844 mL	16.4220 mL	32.8440 mL	82.1099 mL
	5 mM	0.6569 mL	3.2844 mL	6.5688 mL	16.4220 mL
	10 mM	0.3284 mL	1.6422 mL	3.2844 mL	8.2110 mL
	15 mM	0.2190 mL	1.0948 mL	2.1896 mL	5.4740 mL
	20 mM	0.1642 mL	0.8211 mL	1.6422 mL	4.1055 mL
	25 mM	0.1314 mL	0.6569 mL	1.3138 mL	3.2844 mL
	30 mM	0.1095 mL	0.5474 mL	1.0948 mL	2.7370 mL
	40 mM	0.0821 mL	0.4105 mL	0.8211 mL	2.0527 mL
	50 mM	0.0657 mL	0.3284 mL	0.6569 mL	1.6422 mL
	60 mM	0.0547 mL	0.2737 mL	0.5474 mL	1.3685 mL
	80 mM	0.0411 mL	0.2053 mL	0.4105 mL	1.0264 mL
	100 mM	0.0328 mL	0.1642 mL	0.3284 mL	0.8211 mL

Arachidonic acid Related Classifications

Inflammation/Immunology Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Arachidonic acid506-32-1ImmunocytophytEndogenous Metabolitepolyunsaturated fatty acidbiomembranescognitive responsecardiovascular functionInhibitorinhibitorinhibit

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