1. Membrane Transporter/Ion Channel
  2. TRP Channel

Diphenyleneiodonium chloride (Synonyms: DPI)

Cat. No.: HY-100965 Purity: 99.87%
Handling Instructions

Diphenyleneiodonium chloride is a NADPH oxidase (NOX) inhibitor and also functions as a TRPA1 activator with an EC50 of 1 to 3 μM.

For research use only. We do not sell to patients.

Diphenyleneiodonium chloride Chemical Structure

Diphenyleneiodonium chloride Chemical Structure

CAS No. : 4673-26-1

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10 mM * 1 mL in DMSO USD 77 In-stock
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10 mg USD 70 In-stock
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Estimated Time of Arrival: December 31
50 mg USD 240 In-stock
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Estimated Time of Arrival: December 31
100 mg USD 420 In-stock
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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Diphenyleneiodonium chloride is a NADPH oxidase (NOX) inhibitor and also functions as a TRPA1 activator with an EC50 of 1 to 3 μM.

IC50 & Target

NOX[1]
EC50: 1 to 3 μM (TRPA1)[1]

In Vitro

Diphenyleneiodonium chloride is a NADPH oxidase (NOX) inhibitor and also functions as a TRPA1 activator with an EC50 of 1 to 3 μM. Application of Diphenyleneiodonium chloride to HEK-TRPA1 cells at a concentration ranges of 0.03 to 10 μM effectively induces a Ca2+ response. However, Diphenyleneiodonium chloride fails to evoke a Ca2+ response in control HEK cells, even at a relatively high dose of 10 μM[1]. When Diphenyleneiodonium chloride is included in the co-cultures, lipopolysaccharide (LPS)-induced preOL apoptosis is significantly inhibited. Treatment with Diphenyleneiodonium chloride is found to significantly attenuate the LPS-induced O2- production by 2.0-fold, reducing it to within 27% of the controls[2].

In Vivo

Intraplantar injection of 2 mM Diphenyleneiodonium chloride to the hindpaw causes licking or biting behavior[1]. Diphenyleneiodonium chloride treatment immediately or 24 h after lipopolysaccharide (LPS) injection significantly attenuates the LPS-induced loss of O4 positive cells. Treatment with Diphenyleneiodonium chloride either immediately or 24 h after LPS injection significantly ameliorates the LPS-induced disorganization of the white matter nerve fibers. However, treatment with DPI 48 h after LPS injection does not appear to correct the LPS-induced white matter damage. DPI treatment either immediately or 24 h after LPS injection significantly reduces the accumulation of both gp91phox and p67phox in the membrane fraction[2].

Clinical Trial
References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 3.1791 mL 15.8957 mL 31.7915 mL
5 mM 0.6358 mL 3.1791 mL 6.3583 mL
10 mM 0.3179 mL 1.5896 mL 3.1791 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[2]

Purified microglia and preOLs are co-cultured using a Transwell culture system. Co-cultured cells are divided into three groups: control, lipopolysaccharide (LPS)-activated, and LPS plus Diphenyleneiodonium chloride. Microglia are cultured in Transwells over established preOL layers and exposed to either LPS (100 ng/mL), LPS+Diphenyleneiodonium chloride (10 μM) or untreated. The medium supernatants and cellular protein fractions from the co-cultures are then collected for further analysis after 48 h incubation[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

The ddy mice (6 to 7 wk of age) are individually placed in transparent cages for 30 min before experiments. An intraplantar injection of 10 μL Diphenyleneiodonium chloride (2 mM, solvent: Kolliphor EL with 20% DMSO) is then injected into the right hindpaw with or without intraperitoneal administration with HC030031 (300 mg/kg at 0.5 h prior to injection of Diphenyleneiodonium chloride; solvent: saline with 0.5% methyl cellulose). The time spent licking or biting the injected paw is recorded for 45 min after injection[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

314.55

Formula

C₁₂H₈ClI

CAS No.

4673-26-1

SMILES

C12=CC=CC=C1C3=CC=CC=C3[I+]2.[Cl-]

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: 6 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Diphenyleneiodonium chloride
Cat. No.:
HY-100965
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