2-Aminoethyl diphenylborinate
Based on 72 publication(s) in Google Scholar
2-Aminoethyl diphenylborinate (2-APB) is a cell-permeable inhibitor of Inositol triphosphate receptor (IP3R). 2-Aminoethyl diphenylborinate also inhibits the store-operated Ca2+ (SOC) channel and activates some TRP channels (V1, V2 and V3). Additionally, 2-Aminoethyl diphenylborinate has inhibitory effects on vasospasm. At high concentrations, it exhibits specific anti-inflammatory and antioxidant effects in neural tissue.
For research use only. We do not sell to patients.
- Purity: 98.86%
- CAS No.: 524-95-8
- Formula: C14H16BNO
- Molecular Weight:225.09
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Storage:Powder -20°C, 3 years , 4°C, 2 years
* The compound is unstable in solutions, freshly prepared is recommended.
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 2-Aminoethyl diphenylborinate
More- Nat Metab. 2022 Sep;4(9):1185-1201. [Abstract]
- Adv Funct Mater. 2019, 1808556.
- Mol Cell. 2023 Jan 14;S1097-2765(22)01217-5. [Abstract]
- Sci Immunol. 2022 Feb 4;7(68):eabk2092. [Abstract]
- Nat Commun. 2025 Sep 29;16(1):8541. [Abstract]
- Nat Commun. 2022 Nov 10;13(1):6796. [Abstract]
- Acta Pharm Sin B. 2025 Aug;15(8):4078-4095. [Abstract]
- Autophagy. 2024 Nov;20(11):2388-2404. [Abstract]
- Adv Sci (Weinh). 2025 Jul 25:e15313. [Abstract]
- Adv Sci (Weinh). 2024 Jul 23:e2310126. [Abstract]
- Adv Sci (Weinh). 2021 Nov;8(21):e2101936. [Abstract]
- Nat Chem Biol. 2025 Aug;21(8):1283-1291. [Abstract]
- J Nanobiotechnology. 2024 Sep 19;22(1):578. [Abstract]
- Carbohydr Polym. 2022 Feb 1:277:118869. [Abstract]
- Small. 2025 Mar;21(12):e2408139. [Abstract]
- Appl Phys Rev. 2026 Jan 21.
- J Immunother Cancer. 2025 Oct 5;13(10):e012182. [Abstract]
- Cancer Lett. 2023 Nov 28:577:216435. [Abstract]
- Cell Death Dis. 2023 May 13;14(5):325. [Abstract]
- Cell Death Dis. 2021 Apr 1;12(4):336. [Abstract]
- Cell Commun Signal. 2024 Nov 7;22(1):534. [Abstract]
- Phytomedicine. 2024 Apr:126:155200. [Abstract]
- Free Radic Biol Med. 2023 Aug 20:205:25-46. [Abstract]
- J Transl Med. 2025 Nov 10;23(1):1254. [Abstract]
- Environ Pollut. 2024 Feb 15:343:123167. [Abstract]
- J Med Chem. 2025 Apr 17. [Abstract]
- Int J Nanomedicine. 2023 Dec 11:18:7505-7521. [Abstract]
- Ecotoxicol Environ Saf. 2024 Apr 1:274:116218. [Abstract]
- Front Immunol. 2022 May 18:13:835936. [Abstract]
- Front Immunol. 2021 Jul 7:12:688674. [Abstract]
- Bioeng Transl Med. 2025 Dec 5.
- Biochem Pharmacol. 2024 Nov 10;230(Pt 3):116618. [Abstract]
- Chem Biol Interact. 2023 Sep 1:382:110617. [Abstract]
- Pharmaceuticals (Basel). 2026 Mar 23;19(3):520. [Abstract]
- Front Pharmacol. 2024 Jul 31:15:1389761. [Abstract]
- Eur J Pharmacol. 2025 Sep 15:1003:177900. [Abstract]
- Int Immunopharmacol. 2025 May 7:157:114796. [Abstract]
- Toxicology. 2026 Jun:523:154449. [Abstract]
- RSC Adv. 2019 Aug 13;9(43):25107-25118. [Abstract]
- Poult Sci. 2023 Dec;102(12):103013. [Abstract]
- FASEB J. 2019 Sep;33(9):9775-9784. [Abstract]
- Pestic Biochem Physiol. 2026 Jan;216(Pt 1):106798. [Abstract]
- J Cell Physiol. 2024 Apr;239(4):e31190. [Abstract]
- Sci Rep. 2025 Jul 1;15(1):20888. [Abstract]
- Sci Rep. 2025 May 8;15(1):16053. [Abstract]
- ACS Chem Neurosci. 2025 Feb 5;16(3):374-383. [Abstract]
- J Cell Commun Signal. 2023 Dec;17(4):1309-1321. [Abstract]
- J Virol. 2023 May 31;97(5):e0029223. [Abstract]
- Cell Signal. 2019 Jun:58:119-130. [Abstract]
- Vet Res. 2024 Jun 14;55(1):78. [Abstract]
- Food Chem Toxicol. 2022 Oct:168:113407. [Abstract]
- Life Metab. 2025 Jan 29;4(2):loaf002. [Abstract]
- Front Physiol. 2022 Jan 5:12:736920. [Abstract]
- Endocrinology. 2023 Jan 9;164(3):bqac217. [Abstract]
- Hum Exp Toxicol. 2026 Jan-Dec:45:9603271251411659. [Abstract]
- J Inorg Biochem. 2022 Jul;232:111809. [Abstract]
- Insect Sci. 2025 Aug 27. [Abstract]
- Arch Biochem Biophys. 2025 Mar 27:768:110404. [Abstract]
- World J Gastrointest Oncol. 2025 Dec 15;17(12):110736. [Abstract]
- J Nat Med. 2025 Jul;79(4):791-806. [Abstract]
- Biochem Biophys Res Commun. 2025 Apr 1:755:151566. [Abstract]
- Biol Pharm Bull. 2023;46(6):864-868. [Abstract]
- Braz J Med Biol Res. 2025 Apr 14:58:e14590. [Abstract]
- Biocell. 2026 Jan 23.
- bioRxiv. 2026 Jun 23.
- Patent. US20250295610A1.
- SSRN. 2025 Aug 13.
- bioRxiv. 2025 April 03.
- SSRN. 2025 Apr 11.
- bioRxiv. 2024 November 26.
- bioRxiv. 2023 Dec 18.
- SSRN. 2023 Jun 14.
Customer Validation & Images
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Cell Imaging/Staining
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Cell Proliferation/Viability Assay
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Flow Cytometry
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WB
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Cell Proliferation/Viability Assay
All Calcium Channel Isoforms
More
Biological Activity
Inositol triphosphate receptor (IP3R)
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Cell Line
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Type | Value | Description | References |
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| HEK293 | IC50 |
1 μM
Compound: 2-APB
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Inhibition of human TRPM2 expressed in HEK293 cells
Inhibition of human TRPM2 expressed in HEK293 cells
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[PMID: 24304219] |
| Jurkat | IC50 |
15 μM
Compound: 2-APB
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Inhibition of thapsigargin-induced SOCE channel in fura-2 loaded human Jarkart T cells in presence of 2 mM calcium
Inhibition of thapsigargin-induced SOCE channel in fura-2 loaded human Jarkart T cells in presence of 2 mM calcium
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[PMID: 20097561] |
2-Aminoethyl diphenylborinate (2-APB) (10-100 μM, 2 min) evokes external acid-dependent increases of [Ca2+] and dopamine release in PC12 cells through the activation of Orai 3[3].
2-Aminoethyl diphenylborinate (100 μM, 9 h) provides protective effects against ischemia-reperfusion (I/R)-induced damage in Hep G2 cells[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Ischemia-reperfusion-induced testicular injury Sprague Dawley rats model (weighting 250-300 g)[4]
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Dosage:2 or 4 mg/kg
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Administration:Intravenous injection (i.v.) (via portal vein), 10 min before ischemia
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Result:Decreased serum alanine aminotransferase (AST), aspartate aminotransferase (ALT), and lactate dehydrogenase (LDH) following ischemia-reperfusion (I/R).
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Animal Model:Subarachnoid hemorrhage (SAH) Sprague-Dawley rats model (induced by injecting blood from the right femoral artery into the cisterna magna, rats weighting 250-300 g)[2]
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Dosage:0.5 or 2 mg/kg
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Administration:Intraperitoneal (i.p.)
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Result:Increased the basilar artery wall thickness and reduced the basilar artery lumen diameter, inducing significant vascular changes (sampled 24 hours after administration). Alleviated cell apoptosis at 24 hours after subarachnoid hemorrhage (sampled 24 hours after administration).
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Animal Model:Male Sprague Dawley rats model of hepatic Ischemia-reperfusion (I/R) [5]
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Dosage:2 mg/kg
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Administration:Intraperitoneal (i.p.)
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Result:Significantly increased total antioxidant capacity and glutathione levels, and decreased malondialdehyde content, DNA fragmentation, and the number of TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) positive cells (measured 10 minutes after administration).
Chemical Information
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CAS No. 524-95-8
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Appearance Solid
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Molecular Weight 225.09
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Formula C14H16BNO
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Color White to off-white
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SMILES
NCCOB(C1=CC=CC=C1)C2=CC=CC=C2
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Synonyms
2-APB
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years * The compound is unstable in solutions, freshly prepared is recommended.
Publications (72)
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Journal Impact Factor
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Most Recent
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Nat Metab
Orosomucoid 2 maintains hepatic lipid homeostasis through suppression of de novo lipogenesis. [Abstract]2022 Sep;4(9):1185-1201. PMID: 36050503 -
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Mol Cell
Cytosolic DNA sensing by cGAS/STING promotes TRPV2-mediated Ca2+ release to protect stressed replication forks. [Abstract]2023 Jan 14;S1097-2765(22)01217-5. PMID: 36696898 -
Sci Immunol
Epithelial Gasdermin D shapes the host-microbial interface by driving mucus layer formation. [Abstract]2022 Feb 4;7(68):eabk2092. PMID: 35119941 -
Nat Commun
Biomimetic organo-hydrogels reveal the adipose tissue local mechanical anisotropy regulates ovarian cancer invasion. [Abstract]2025 Sep 29;16(1):8541. PMID: 41022685 -
Nat Commun
Maprotiline restores ER homeostasis and rescues neurodegeneration via Histamine Receptor H1 inhibition in retinal ganglion cells. [Abstract]2022 Nov 10;13(1):6796. PMID: 36357388 -
Acta Pharm Sin B
2025 Aug;15(8):4078-4095. PMID: 40893681 -
Autophagy
Activation of BK channels prevents diabetes-induced osteopenia by regulating mitochondrial Ca2+ and SLC25A5/ANT2-PINK1-PRKN-mediated mitophagy. [Abstract]2024 Nov;20(11):2388-2404. PMID: 38873928 -
Adv Sci (Weinh)
Activation of Lysosomal Retrograde Transport Triggers TPC1-IP3R1 Ca2+ Crosstalk at Lysosome-ER MCSs Leading to Lethal Depleting of ER Calcium. [Abstract]2025 Jul 25:e15313. PMID: 40709664 -
Adv Sci (Weinh)
Targeting Transient Receptor Potential Melastatin-2 (TRPM2) Enhances Therapeutic Efficacy of Third Generation EGFR Inhibitors against EGFR Mutant Lung Cancer. [Abstract]2024 Jul 23:e2310126. PMID: 39044361 -
Adv Sci (Weinh)
A Novel Mechanism of Endoplasmic Reticulum Stress- and c-Myc-Degradation-Mediated Therapeutic Benefits of Antineurokinin-1 Receptor Drugs in Colorectal Cancer. [Abstract]2021 Nov;8(21):e2101936. PMID: 34605226
2-Aminoethyl diphenylborinate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2021 Nov;8(21):e2101936. [Abstract]
HCT116 cells were pretreated with 2-APB (10 × 10−6 M) for 1 h, followed by SR140333 treatment for 24 h. cell viability was measured by trypan blue exclusion assay.
2-Aminoethyl diphenylborinate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2021 Nov;8(21):e2101936. [Abstract]
Western blotting in HCT116 cells treated with SR140333 (38 × 10−6 m) for 24 h after pretreatment with BAPTA-AM (10 ×10−6 M) or 2-APB (10 × 10−6 M) for 1 h.
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Nat Chem Biol
Eukaryotic ADCY7 catalyzes the production of c-di-AMP to activate the NLRP3 inflammasome. [Abstract]2025 Aug;21(8):1283-1291. PMID: 40419769 -
J Nanobiotechnology
Macrophage membrane-camouflaged biomimetic nanoparticles for rheumatoid arthritis treatment via modulating macrophage polarization. [Abstract]2024 Sep 19;22(1):578. PMID: 39300463
2-Aminoethyl diphenylborinate purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Sep 19;22(1):578. [Abstract]
The effect of 2-APB (0, 25, 50, 100 μM; 2+24 h) on the expression of polarization marker proteins iNOS and Arg-1 in RAW264.7 cells after LPS/IL-4 treatment.
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Carbohydr Polym
Chitosan oligosaccharide regulates AMPK and STAT1 pathways synergistically to mediate PD-L1 expression for cancer chemoimmunotherapy. [Abstract]2022 Feb 1:277:118869. PMID: 34893274 -
Small
2025 Mar;21(12):e2408139. PMID: 40012250 -
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J Immunother Cancer
Macrophage NLRP3 activation and IL-1β release drive osimertinib-induced antitumor immunity. [Abstract]2025 Oct 5;13(10):e012182. PMID: 41052880
2-Aminoethyl diphenylborinate purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2025 Oct 5;13(10):e012182. [Abstract]
Confocal microscopy analysis of cellular calcium overloading in BMDMs after osimertinib stimulation with or without ML-SI3 (5µM) or 2-APB (10µM; 30 min) pretreatment.
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Cancer Lett
2023 Nov 28:577:216435. PMID: 37806516 -
Cell Death Dis
Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats. [Abstract]2023 May 13;14(5):325. PMID: 37179386 -
Cell Death Dis
RANK promotes colorectal cancer migration and invasion by activating the Ca2+-calcineurin/NFATC1-ACP5 axis. [Abstract]2021 Apr 1;12(4):336. PMID: 33795653 -
Cell Commun Signal
Inhibition of SGLT2 protects podocytes in diabetic kidney disease by rebalancing mitochondria-associated endoplasmic reticulum membranes. [Abstract]2024 Nov 7;22(1):534. PMID: 39511548 -
Phytomedicine
Sinapine targeting PLCβ3 EF hands disrupts Gαq-PLCβ3 interaction and ameliorates cardiovascular diseases. [Abstract]2024 Apr:126:155200. PMID: 38387273 -
Free Radic Biol Med
β-carotene targets IP3R/GRP75/VDAC1-MCU axis to renovate LPS-induced mitochondrial oxidative damage by regulating STIM1. [Abstract]2023 Aug 20:205:25-46. PMID: 37270031 -
J Transl Med
Peroxynitrite regulates ER stress-mediated Ca2+ flux to mitochondria characterizing cardiac microvascular ischemia-reperfusion injury associated with hyperhomocysteinemia. [Abstract]2025 Nov 10;23(1):1254. PMID: 41214689
2-Aminoethyl diphenylborinate purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Nov 10;23(1):1254. [Abstract]
Effect of different calcium ion channel blockers oncell death. HCMEC were pretreated with Dizocilpine (10 µM), Nifedipine (10 µM) and 2-APB (100 µM) for 1 h and then treated with Hcy and CuCl2 underH/R condition for 24 h. Cell death was analyzed by LDH release assay
2-Aminoethyl diphenylborinate purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Nov 10;23(1):1254. [Abstract]
HCMECs were pretreated with 2-APB (100 µM) for 1 h and then treated with Hcy and CuCl2 under H/R condition for 24 h. Effect of 2-APB on total intracellular and mitochondrial calcium.
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Environ Pollut
Perturbation of IP3R-dependent endoplasmic reticulum calcium homeostasis by PPARδ-activated metabolic stress leads to mouse spermatocyte apoptosis: A direct mechanism for perfluorooctane sulfonic acid-induced spermatogenic disorders. [Abstract]2024 Feb 15:343:123167. PMID: 38110051 -
J Med Chem
A Novel Compound 3a-M1, from Metabolites of Sinomenine Derivative 3a, Exerts Potent Anti-Aplastic Anemia Activity via IP3R/ORAI-Mediated CTL Ferroptosis. [Abstract]2025 Apr 17. PMID: 40243551 -
Int J Nanomedicine
Intravenous Injection of Na Ions Aggravates Ang II-Induced Hypertension-Related Vascular Endothelial Injury by Increasing Transmembrane Osmotic Pressure. [Abstract]2023 Dec 11:18:7505-7521. PMID: 38106448 -
Ecotoxicol Environ Saf
Cyfluthrin exposure during pregnancy causes neurotoxicity in offspring-Ca2+ overload via IP3R-GRP75-VDAC1 pathway. [Abstract]2024 Apr 1:274:116218. PMID: 38492481 -
Front Immunol
Intracellular Ca2+ Signaling and Calcium Release-Activated Calcium Modulator ORAI1 Are Associated With CD4+ T Lymphocytes in Dairy Cows. [Abstract]2022 May 18:13:835936. PMID: 35663942 -
Front Immunol
Inflammasome-Induced Osmotic Pressure and the Mechanical Mechanisms Underlying Astrocytic Swelling and Membrane Blebbing in Pyroptosis. [Abstract]2021 Jul 7:12:688674. PMID: 34305921 -
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Biochem Pharmacol
3,3'-Diindolylmethane promotes bone formation - A assessment in MC3T3-E1 cells and zebrafish. [Abstract]2024 Nov 10;230(Pt 3):116618. PMID: 39528071 -
Chem Biol Interact
Molybdenum and/or cadmium induce NLRP3 inflammasome production by causing mitochondria-associated endoplasmic reticulum membrane dysfunction in sheep hepatocytes. [Abstract]2023 Sep 1:382:110617. PMID: 37385403 -
Pharmaceuticals (Basel)
Danggui Buxue Decoction and Its Active Constituents Inhibit Drug-Induced Uterine Contractions via L-Type Calcium Channels and the IP3/Ca2+ Pathway. [Abstract]2026 Mar 23;19(3):520. PMID: 41901365 -
Front Pharmacol
N-demethylsinomenine metabolite and its prototype sinomenine activate mast cells via MRGPRX2 and aggravate anaphylaxis. [Abstract]2024 Jul 31:15:1389761. PMID: 39144634 -
Eur J Pharmacol
Novel mechanisms of metformin-induced vasorelaxation of mesenteric arterioles via endothelium-dependent hyperpolarization to treat murine colitis. [Abstract]2025 Sep 15:1003:177900. PMID: 40617384 -
Int Immunopharmacol
OPTN ameliorates chondrocyte apoptosis in temporomandibular joint osteoarthritis by modulating ER-mitochondria Ca2+ transfer. [Abstract]2025 May 7:157:114796. PMID: 40339496 -
Toxicology
Co-exposure to polystyrene nanoplastics and cadmium induces apoptosis in intestinal cells: Role of the IP3R/Ca²⁺/STAT3 signaling pathway. [Abstract]2026 Jun:523:154449. PMID: 41833704 -
RSC Adv
Ginsenoside Rk1 inhibits cell proliferation and promotes apoptosis in lung squamous cell carcinoma by calcium signaling pathway. [Abstract]2019 Aug 13;9(43):25107-25118. PMID: 35528653 -
Poult Sci
Inhibition of calcium imbalance protects hepatocytes from vanadium exposure-induced inflammation by mediating mitochondrial-associated endoplasmic reticulum membranes in ducks. [Abstract]2023 Dec;102(12):103013. PMID: 37856907 -
FASEB J
2019 Sep;33(9):9775-9784. PMID: 31166814 -
Pestic Biochem Physiol
Three stress-responsive peptides modulate immunity and development in the fall armyworm Spodoptera frugiperda. [Abstract]2026 Jan;216(Pt 1):106798. PMID: 41326125 -
J Cell Physiol
By regulating the IP3R/GRP75/VDAC1 complex to restore mitochondrial dynamic balance, selenomethionine reduces lipopolysaccharide-induced neuronal apoptosis. [Abstract]2024 Apr;239(4):e31190. PMID: 38219075 -
Sci Rep
High-intensity light disrupts intracellular organelle dynamics via microtubule depolymerization. [Abstract]2025 Jul 1;15(1):20888. PMID: 40593938 -
Sci Rep
Estrogen regulates duodenal calcium absorption and improves postmenopausal osteoporosis by the effect of ERβ on PMCA1b. [Abstract]2025 May 8;15(1):16053. PMID: 40341576 -
ACS Chem Neurosci
2025 Feb 5;16(3):374-383. PMID: 39800970 -
J Cell Commun Signal
Insights into the mediation of Ca2+ signaling in the promoting effects of LETX-VI on the synthesis and release of dopamine. [Abstract]2023 Dec;17(4):1309-1321. PMID: 37702818 -
J Virol
Coronavirus Porcine Epidemic Diarrhea Virus Utilizes Chemokine Interleukin-8 to Facilitate Viral Replication by Regulating Ca2+ Flux. [Abstract]2023 May 31;97(5):e0029223. PMID: 37133374 -
Cell Signal
2019 Jun:58:119-130. PMID: 30849520 -
Vet Res
Regulatory effects of Trichinella spiralis serpin-type serine protease inhibitor on endoplasmic reticulum stress and oxidative stress in host intestinal epithelial cells. [Abstract]2024 Jun 14;55(1):78. PMID: 38877574 -
Food Chem Toxicol
Chronic glucocorticoid exposure accelerates Aβ generation and neurotoxicity by activating calcium-mediated CN-NFAT1 signaling in hippocampal neurons in APP/PS1 mice. [Abstract]2022 Oct:168:113407. PMID: 36075474 -
Life Metab
Intermittent fasting inhibits platelet activation and thrombosis through the intestinal metabolite indole-3-propionate. [Abstract]2025 Jan 29;4(2):loaf002. PMID: 40078933 -
Front Physiol
Endoplasmic Reticulum Stress/Ca2+-Calmodulin-Dependent Protein Kinase/Signal Transducer and Activator of Transcription 3 Pathway Plays a Role in the Regulation of Cellular Zinc Deficiency in Myocardial Ischemia/Reperfusion Injury. [Abstract]2022 Jan 5:12:736920. PMID: 35069232 -
Endocrinology
Aspirin Suppresses Hepatic Glucagon Signaling Through Decreasing Production of Thromboxane A2. [Abstract]2023 Jan 9;164(3):bqac217. PMID: 36592127 -
Hum Exp Toxicol
Inhibition of store-operated calcium entry mitigates lipopolysaccharide-induced hepatotoxicity via modulating inflammatory and oxidative stress responses. [Abstract]2026 Jan-Dec:45:9603271251411659. PMID: 41508650 -
J Inorg Biochem
Endoplasmic reticulum-mitochondria coupling attenuates vanadium-induced apoptosis via IP3R in duck renal tubular epithelial cells. [Abstract]2022 Jul;232:111809. PMID: 35421768 -
Insect Sci
Asynchronous programmed cell death occurrence in the silk glands at pupation (Bombyx mori). [Abstract]2025 Aug 27. PMID: 40867085 -
Arch Biochem Biophys
The odorant (R)-(-)-carvone promotes glucose-stimulated insulin secretion via the olfactory receptor Olfr1259 in pancreatic β-TC6 cells. [Abstract]2025 Mar 27:768:110404. PMID: 40157529 -
World J Gastrointest Oncol
Transient receptor potential melastatin 6 and transient receptor potential melastatin 6/7 antagonists suppress colon adenocarcinoma HT-29 cells. [Abstract]2025 Dec 15;17(12):110736. PMID: 41480213 -
J Nat Med
Ginsenoside Rh3-induced neurotoxicity involving the IP3R-Ca2+/NOX2/NF-κB signaling pathways. [Abstract]2025 Jul;79(4):791-806. PMID: 40360886 -
Biochem Biophys Res Commun
TRPC1 downregulation enhances catecholamine secretion in adrenal chromaffin cells under metabolic syndrome conditions. [Abstract]2025 Apr 1:755:151566. PMID: 40043610 -
Biol Pharm Bull
Inhibition of TRPC4/5 Channel Is Effective in Protecting against Histamine-Induced Hyperpermeability by Blocking Ca2+ Influx in Lung Microvascular Endothelial Cells. [Abstract]2023;46(6):864-868. PMID: 37258152 -
Braz J Med Biol Res
Oleanolic acid enhanced the anticancer effect of fluorouracil by regulating Ca2+ levels in hepatocellular carcinoma cells. [Abstract]2025 Apr 14:58:e14590. PMID: 40243820 -
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Solvent & Solubility
DMSO : 250 mg/mL (1110.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 1.61 mg/mL (7.15 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (9.24 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (9.24 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * The compound is unstable in solutions, freshly prepared is recommended.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Ma S, et al. Effect of Wilforine on the Calcium Signaling Pathway in Mythimna separata Walker MyocytesUsing the Calcium Imaging Technique. J Agric Food Chem. 2019 Nov 27. [Content Brief]
[2]. Boyacı MG, et al. Effects of 2-Aminoethyl Diphenylborinate, a Modulator of Transient Receptor Potential and OraiChannels in Subarachnoid Hemorrhage: An Experimental Study. [Content Brief]
[3]. Takahashi K, et al. Molecular mechanism of 2-APB-induced Ca²⁺ influx in external acidification in PC12. Exp Cell Res. 2014 May 1;323(2):337-45. [Content Brief]
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Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 4.4427 mL | 22.2133 mL | 44.4267 mL | 111.0667 mL |
| 5 mM | 0.8885 mL | 4.4427 mL | 8.8853 mL | 22.2133 mL | |
| DMSO | 10 mM | 0.4443 mL | 2.2213 mL | 4.4427 mL | 11.1067 mL |
| 15 mM | 0.2962 mL | 1.4809 mL | 2.9618 mL | 7.4044 mL | |
| 20 mM | 0.2221 mL | 1.1107 mL | 2.2213 mL | 5.5533 mL | |
| 25 mM | 0.1777 mL | 0.8885 mL | 1.7771 mL | 4.4427 mL | |
| 30 mM | 0.1481 mL | 0.7404 mL | 1.4809 mL | 3.7022 mL | |
| 40 mM | 0.1111 mL | 0.5553 mL | 1.1107 mL | 2.7767 mL | |
| 50 mM | 0.0889 mL | 0.4443 mL | 0.8885 mL | 2.2213 mL | |
| 60 mM | 0.0740 mL | 0.3702 mL | 0.7404 mL | 1.8511 mL | |
| 80 mM | 0.0555 mL | 0.2777 mL | 0.5553 mL | 1.3883 mL | |
| 100 mM | 0.0444 mL | 0.2221 mL | 0.4443 mL | 1.1107 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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