Protective effect of 2-APB on testicular ischemia-reperfusion injury in rats

  • J Urol. 2015 Mar;193(3):1036-41. doi: 10.1016/j.juro.2014.09.120.
Erhan Sari  1 ,  Hasan Aksit  2 ,  Haydar Ali Erken  3 ,  Arzu Yay  4 ,  Dilek Aksit  5 ,  Akin Soner Amasyali  6 ,  Onur Yildiz  2 ,  Ahmet Kucukyangoz  7 ,  Derya Akkus  4 ,  Kamil Seyrek  8
Affiliations
  • 1. Department of Urology, Faculty of Medicine, Veterinary Faculty, Balikesir University, Balikesir, Turkey. Electronic address: [email protected].
  • 2. Department of Biochemistry, Veterinary Faculty, Balikesir University, Balikesir, Turkey.
  • 3. Department of Physiology, Faculty of Medicine, Veterinary Faculty, Balikesir University, Balikesir, Turkey.
  • 4. Department of Histology and Embryology, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
  • 5. Department of Pharmacology, Veterinary Faculty, Balikesir University, Balikesir, Turkey.
  • 6. Department of Urology, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey.
  • 7. Department of Urology, Faculty of Medicine, Veterinary Faculty, Balikesir University, Balikesir, Turkey.
  • 8. Department of Biochemistry, Faculty of Medicine, Veterinary Faculty, Balikesir University, Balikesir, Turkey.
PMID: 25444954 DOI: 10.1016/j.juro.2014.09.120
Abstract

Purpose: We performed biochemical and histopathological evaluations to assess the effects of 2-APB on ischemia-reperfusion induced testicular damage.

Materials and methods: A total of 28 rats were randomly divided into 4 groups, including sham treated, ischemia-reperfusion, ischemia-reperfusion plus 2 mg/kg 2-APB and ischemia-reperfusion plus 4 mg/kg 2-APB. Testicular tissue superoxide dismutase, glutathione, malondialdehyde, total antioxidant capacity and DNA fragmentation levels were determined. Testicular tissue samples were examined by histopathology and TUNEL staining.

Results: Mean superoxide dismutase, total antioxidant capacity and glutathione were significantly higher in the sham treated group than in the ischemia-perfusion group (p <0.05). Mean malondialdehyde and DNA fragmentation levels were significantly lower in the sham treated group than in the ischemia-reperfusion group (p <0.05). After 2-APB treatment superoxide dismutase, total antioxidant capacity and glutathione were significantly increased but malondialdehyde and DNA fragmentation levels were significantly decreased compared to the ischemia-reperfusion group (p <0.05). The number of TUNEL positive cells was significantly lower in the 2-APB treatment groups than in the ischemia-reperfusion group (p <0.05).

Conclusions: In rats 2-APB reduced the oxidative stress and Apoptosis caused by testicular ischemia-reperfusion injury. The testicular protective effect of 2-APB appears to be mediated through its antiapoptotic and antioxidative effects.

Keywords
2-aminoethoxydiphenyl borate; apoptosis; oxidative stress; reperfusion injury; testis.
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