Cat. No.: HY-12542

Dantrolene

(Synonyms: F 368)

15 Cited Publications

Customer Review

Based on 15 publication(s) in Google Scholar

Dantrolene is an orally active, non-competitive glutathione reductase inhibitor with a K_i of 111.6 μM and an IC₅₀ of 52.3 μM. Dantrolene is also a calcium channel protein inhibitor. Dantrolene inhibits the release of Ca²⁺ from RyR1 and RyR3, which can be beneficial in a variety of pathologies caused by disruptions in calcium homeostasis (e.g., stroke, ischemia/reperfusion injury, and neurodegenerative diseases). Dantrolene offers relief of muscle spasms, malignant hyperthermia, and antitoxic, antipyretic, and anti-inflammatory properties.

For research use only. We do not sell to patients.

Purity: 99.93%
CAS No.: 7261-97-4
Formula: C₁₄H₁₀N₄O₅
Molecular Weight:314.26

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Dantrolene Chemical Structure

CAS No.: 7261-97-4

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (15)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All Calcium Channel Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	0.059 μM Compound: Dantrolene	Inhibition of RyR1 harboring R2163C mutant (unknown origin) stably expressed in HEK293 cells coexpressing R-CEPIA1er assessed as suppression in Ca2+ leakage from ER prestimulated with doxycycline for 24 to 28 hrs followed by compound addition at 100 secs Inhibition of RyR1 harboring R2163C mutant (unknown origin) stably expressed in HEK293 cells coexpressing R-CEPIA1er assessed as suppression in Ca2+ leakage from ER prestimulated with doxycycline for 24 to 28 hrs followed by compound addition at 100 secs	[PMID: 31299492]

In Vitro

Dantrolene (50 μM) sodium alleviates malignant hyperthermia by preventing overactive Ca²⁺ release by increasing the affinity of ryanodine receptor (RyR) for Mg²⁺ in rat skin fibers^[2].
Dantrolene (30 μM, 1 h-3 days) sodium ameliorates the impairment of neurogenesis and synaptogenesis, in association with restoring intracellular Ca²⁺ homeostasis and physiological autophagy, cell survival and proliferation in induced pluripotent stem cells derived from patients with Alzheimer's disease ^[3].
Dantrolene (40 μM, 12 h) sodium enhances the protective effect of hypothermia on cerebral cortex neurons, including improved morphology, increased survival and mitochondrial membrane potential, decreased intracellular reactive oxygen species, cytoplasmic histone-associated DNA fragmentation and apoptosis^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Differentiation Assay^[3]

Cell Line:

Induced pluripotent stem cells from patients with familiar and sporadic Alzheimer’s disease
Concentration:

30 μM
Incubation Time:

3 days
Result:

Promoted differentiation of neuroprogenitor cell derived from sporadic Alzheimer’s disease/familial Alzheimer’s disease induced pluripotent stem cells into immature neurons at differentiation day 23.
Promoted the percentage of Trb1 positive cells.
Promoted differentiation into basal forebrain cholinergic neurons.

In Vivo

Dantrolene (5-20 mg/kg, i.v.) sodium exhibits cardioprotective effects in a rat model of Digoxin (HY-B1049)-induced cardiotoxicity^[4].
Dantrolene (5-10 mg/kg, i.p.) sodium attenuates spinal cord inflammation in mice with experimental autoimmune encephalomyelitis (EAE) ^[5].
Dantrolene (5 mg/kg, intranasal or subcutaneous, 3 times per week) sodium improves cognitive dysfunction in 5XFAD Alzheimer's disease (AD) mice ^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6 male mice (EAE was induced by subcutaneous administration of a total of 300 μg in the flanks (each flank received 150 μg) of rodent myelin oligodendrocyte glycoprotein peptide 35–55 (MEVGWYRSPFSRVVHLYRNGK, New England Peptide) emulsified in the complete Freund’s adjuvant containing Mycobacterium tuberculosis H37Ra^[5]
Dosage:

5, 10 mg/kg
Administration:

Intraperitoneal injection (i.p.)
Result:

Reduced the severity of EAE clinical symptoms and dampened inflammation in the spinal cord
Caused skeletal muscle weakness at 5 mg/kg.
Did not display noticeable upright movement deficits on flat-surface at 5 mg/kg.

Chemical Information

CAS No. 7261-97-4
Appearance Solid
Molecular Weight 314.26
Formula C₁₄H₁₀N₄O₅
Color Light yellow to yellow
SMILES

O=C1NC(CN1/N=C/C2=CC=C(C3=CC=C([N+]([O-])=O)C=C3)O2)=O
Synonyms

F 368
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (15)

All Bio/Physico-chemical Assay

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Journal Impact Factor

Cell Res

Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate. [Abstract]

2022 Mar;32(3):288-301. PMID: 35121857
Nat Commun

Crystal structures of Ryanodine Receptor reveal dantrolene and azumolene interactions guiding inhibitor development. [Abstract]

2025 Nov 18;16(1):10110. PMID: 41253812

Dantrolene purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10110. [Abstract]

Dose-dependent effects of Dantrolene (DAN) and AZU on ER luminal [Ca²⁺] changes in cell lines expressing RyR1 R2163C. The reslts showed that effectively Dantrolene (DAN) inhibited Ca²⁺ leakage, with IC₅₀ value of 0.26 μM.
Anim Nutr

Arginine promotes myogenic differentiation and myotube formation through the elevation of cytoplasmic calcium concentration. [Abstract]

2021 Dec;7(4):1115-1123. PMID: 34738042
Cell Rep

Carvedilol sensitizes chemotherapy by targeting STING to boost anti-tumor immunity. [Abstract]

2025 Apr 16;44(5):115572. PMID: 40249703
J Med Chem

A Novel Compound 3a-M1, from Metabolites of Sinomenine Derivative 3a, Exerts Potent Anti-Aplastic Anemia Activity via IP3R/ORAI-Mediated CTL Ferroptosis. [Abstract]

2025 Apr 17. PMID: 40243551
J Anim Sci Biotechnol

Ryanodine receptor RyR1-mediated elevation of Ca2+ concentration is required for the late stage of myogenic differentiation and fusion. [Abstract]

2022 Feb 11;13(1):9. PMID: 35144690
Front Immunol

Inflammasome-Induced Osmotic Pressure and the Mechanical Mechanisms Underlying Astrocytic Swelling and Membrane Blebbing in Pyroptosis. [Abstract]

2021 Jul 7:12:688674. PMID: 34305921
Biochem Pharmacol

Dual inhibition of EGR1/STAT3 transcriptional hubs suppresses macrophage-driven liver fibrosis: A multi-omics-guided drug repurposing strategy. [Abstract]

2025 Oct:240:117120. PMID: 40623460
Thromb Haemost

Inhibition of IP3 (Inositol 1,4,5-Trisphosphate) Receptors Retards SARS-CoV-2-Induced Endothelial von Willebrand Factor Secretion and Thrombosis. [Abstract]

2025 Mar 6. PMID: 40049586
ACS Omega

Machine Learning for Discovery of GSK3β Inhibitors. [Abstract]

2020 Oct 12;5(41):26551-26561. PMID: 33110983
J Cell Commun Signal

Insights into the mediation of Ca2+ signaling in the promoting effects of LETX-VI on the synthesis and release of dopamine. [Abstract]

2023 Dec;17(4):1309-1321. PMID: 37702818

Dantrolene purchased from MedChemExpress. Usage Cited in: J Cell Commun Signal. 2023 Dec;17(4):1309-1321. [Abstract]

Effect of 2 µM LETX-VI treatment on cytosolic Ca²⁺ level when RyRs in ER membrane was inhibited by Dantrolene (30 µM). The results demonstrated that blockade of RyR by Dantrolene decreased the F340/F380 ratio, indicating that RyR is involved in LETX-VI-induced Ca²⁺ release from the endoplasmic reticulum.
Biocell

2026 Jan 23.
bioRxiv

Angiotensin II elicits robust calcium oscillations coordinated within juxtaglomerular cell clusters to suppress renin secretion. [Abstract]

2024 Dec 23:2024.12.23.629519. PMID: 39763801
Res Sq

2024 Jun 11.
Research Square Preprint

2021 May.

Solvent & Solubility

In Vitro:

DMSO : 16.67 mg/mL (53.05 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.1821 mL

15.9104 mL

31.8208 mL

5 mM

0.6364 mL

3.1821 mL

6.3642 mL

10 mM

0.3182 mL

1.5910 mL

3.1821 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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In Vivo:

The following protocol is derived from the literature and is for reference only. It is recommended to first try a small sample.

Protocol 1

Dantrolene is suspended in 50 μL of PBS with 2% corn flour^[5].

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zhang W, et al. Neuroprotective effects of dantrolene in neurodegenerative disease: Role of inhibition of pathological inflammation. J Anesth Transl Med. 2024 Jun;3(2):27-35. [Content Brief]

[2]. Choi RH, et al. Dantrolene requires Mg2+ to arrest malignant hyperthermia. Proc Natl Acad Sci U S A. 2017 May 2;114(18):4811-4815. [Content Brief]

[3]. Wang Y, et al. Dantrolene Ameliorates Impaired Neurogenesis and Synaptogenesis in Induced Pluripotent Stem Cell Lines Derived from Patients with Alzheimer's Disease. Anesthesiology. 2020 May;132(5):1062-1079. [Content Brief]

[4]. Zardast M, et al. Evaluation of the Effectiveness of Dantrolene against Digoxininduced Cardiotoxicity in Adult Rats. Cardiovasc Hematol Agents Med Chem. 2023;22(1):60-65. [Content Brief]

[5]. Osipchuk NC, et al. Modulation of Ryanodine Receptors Activity Alters the Course of Experimental Autoimmune Encephalomyelitis in Mice. Front Physiol. 2021 Dec 17;12:770820. [Content Brief]

[6]. Shi Y, et al. Intranasal Dantrolene as a Disease-Modifying Drug in Alzheimer 5XFAD Mice. J Alzheimers Dis. 2020;76(4):1375-1389. [Content Brief]

[7]. Xu SY, et al. Dantrolene enhances the protective effect of hypothermia on cerebral cortex neurons. Neural Regen Res. 2015 Aug;10(8):1279-85. [Content Brief]

[8]. F Zhao, et al. Dantrolene inhibition of ryanodine receptor Ca2+ release channels. Molecular mechanism and isoform selectivity. J Biol Chem. 2001 Apr 27;276(17):13810-6. [Content Brief]

[9]. Christopher B Sylvester, et al. Dantrolene inhibits lysophosphatidylcholine-induced valve interstitial cell calcific nodule formation via blockade of the ryanodine receptor. Front Cardiovasc Med. 2023 Mar 30:10:1112965. [Content Brief]

[10]. Xi Chen, et al. Dantrolene is neuroprotective in Huntington's disease transgenic mouse model. Mol Neurodegener. 2011 Nov 25;6:81. [Content Brief]

[11]. Büyükokuroğlu ME. Anti-inflammatory and antinociceptive properties of Dantrolene in rats and mice. Pharmacol Res. 2002 Jun;45(6):455-60. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.1821 mL	15.9104 mL	31.8208 mL	79.5520 mL
	5 mM	0.6364 mL	3.1821 mL	6.3642 mL	15.9104 mL
	10 mM	0.3182 mL	1.5910 mL	3.1821 mL	7.9552 mL
	15 mM	0.2121 mL	1.0607 mL	2.1214 mL	5.3035 mL
	20 mM	0.1591 mL	0.7955 mL	1.5910 mL	3.9776 mL
	25 mM	0.1273 mL	0.6364 mL	1.2728 mL	3.1821 mL
	30 mM	0.1061 mL	0.5303 mL	1.0607 mL	2.6517 mL
	40 mM	0.0796 mL	0.3978 mL	0.7955 mL	1.9888 mL
	50 mM	0.0636 mL	0.3182 mL	0.6364 mL	1.5910 mL

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Dantrolene

Apoptosis Compound Library

Immunology/Inflammation Compound Library

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Publications Citing Use of MedChemExpress (MCE) Dantrolene

All Calcium Channel Isoforms

Biological Activity

Chemical Information

Publications (15)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Dantrolene Related Classifications

Cat. No.: HY-12542