Cat. No.: HY-12542A

Dantrolene sodium hemiheptahydrate

Name: Dantrolene sodium hemiheptahydrate
Brand: MedChemExpress
SKU: HY-12542A
Price: 60.00 USD
Availability: InStock
Rating: 5 (17 reviews)

(Synonyms: Dantrolene sodium hydrate)

17 Cited Publications

Customer Review

Based on 17 publication(s) in Google Scholar

Dantrolene sodium hemiheptahydrate is an orally active, non-competitive glutathione reductase inhibitor with a K_i of 111.6 μM and an IC₅₀ of 52.3 μM. Dantrolene sodium hemiheptahydrate is also a calcium channel protein inhibitor. Dantrolene sodium hemiheptahydrate inhibits the release of Ca²⁺ from RyR1 and RyR3, which can be beneficial in a variety of pathologies caused by disruptions in calcium homeostasis (e.g., stroke, ischemia/reperfusion injury, and neurodegenerative diseases). Dantrolene sodium hemiheptahydrate offers relief of muscle spasms, malignant hyperthermia, and antitoxic, antipyretic, and anti-inflammatory properties.

For research use only. We do not sell to patients.

Purity: 99.79%
CAS No.: 24868-20-0
Formula: C₁₄H₁₆N₄NaO_8.5
Molecular Weight:399.31

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Dantrolene sodium hemiheptahydrate Chemical Structure

CAS No.: 24868-20-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (17)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Calcium Channel Isoforms :

In Vitro

Dantrolene (50 μM) sodium hemiheptahydrate alleviates malignant hyperthermia by preventing overactive Ca²⁺ release by increasing the affinity of ryanodine receptor (RyR) for Mg²⁺ in rat skin fibers^[2].
Dantrolene (30 μM, 1 h-3 days) sodium hemiheptahydrate ameliorates the impairment of neurogenesis and synaptogenesis, in association with restoring intracellular Ca²⁺ homeostasis and physiological autophagy, cell survival and proliferation in induced pluripotent stem cells derived from patients with Alzheimer's disease ^[3].
Dantrolene (40 μM, 12 h) sodium hemiheptahydrate enhances the protective effect of hypothermia on cerebral cortex neurons, including improved morphology, increased survival and mitochondrial membrane potential, decreased intracellular reactive oxygen species, cytoplasmic histone-associated DNA fragmentation and apoptosis^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Differentiation Assay^[3]

Cell Line:

Induced pluripotent stem cells from patients with familiar and sporadic Alzheimer’s disease
Concentration:

30 μM
Incubation Time:

3 days
Result:

Promoted differentiation of neuroprogenitor cell derived from sporadic Alzheimer’s disease/familial Alzheimer’s disease induced pluripotent stem cells into immature neurons at differentiation day 23.
Promoted the percentage of Trb1 positive cells.
Promoted differentiation into basal forebrain cholinergic neurons.

In Vivo

Dantrolene (5-20 mg/kg, i.v.) sodium hemiheptahydrate exhibits cardioprotective effects in a rat model of Digoxin (HY-B1049)-induced cardiotoxicity^[4].
Dantrolene (5-10 mg/kg, i.p.) sodium hemiheptahydrate attenuates spinal cord inflammation in mice with experimental autoimmune encephalomyelitis (EAE) ^[5].
Dantrolene (5 mg/kg, intranasal or subcutaneous, 3 times per week) sodium hemiheptahydrate improves cognitive dysfunction in 5XFAD Alzheimer's disease (AD) mice ^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6 male mice (EAE was induced by subcutaneous administration of a total of 300 μg in the flanks (each flank received 150 μg) of rodent myelin oligodendrocyte glycoprotein peptide 35–55 (MEVGWYRSPFSRVVHLYRNGK, New England Peptide) emulsified in the complete Freund’s adjuvant containing Mycobacterium tuberculosis H37Ra^[5]
Dosage:

5, 10 mg/kg
Administration:

Intraperitoneal injection (i.p.)
Result:

Reduced the severity of EAE clinical symptoms and dampened inflammation in the spinal cord
Caused skeletal muscle weakness at 5 mg/kg.
Did not display noticeable upright movement deficits on flat-surface at 5 mg/kg.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 24868-20-0
Appearance Solid
Molecular Weight 399.31
Formula C₁₄H₁₆N₄NaO_8.5
Color Yellow to orange
SMILES

O=C1N([Na])C(CN1/N=C/C2=CC=C(C3=CC=C([N+]([O-])=O)C=C3)O2)=O.[3.5H2O]
Synonyms

Dantrolene sodium hydrate
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (17)

All Bio/Physico-chemical Assay

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Journal Impact Factor

Cell Res

Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate. [Abstract]

2022 Mar;32(3):288-301. PMID: 35121857
Nat Commun

Crystal structures of Ryanodine Receptor reveal dantrolene and azumolene interactions guiding inhibitor development. [Abstract]

2025 Nov 18;16(1):10110. PMID: 41253812

Dantrolene sodium hemiheptahydrate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10110. [Abstract]

Dose-dependent effects of Dantrolene (DAN) and AZU on ER luminal [Ca²⁺] changes in cell lines expressing RyR1 R2163C. The reslts showed that effectively Dantrolene (DAN) inhibited Ca²⁺ leakage, with IC₅₀ value of 0.26 μM.
Anim Nutr

Arginine promotes myogenic differentiation and myotube formation through the elevation of cytoplasmic calcium concentration. [Abstract]

2021 Dec;7(4):1115-1123. PMID: 34738042
J Anim Sci Biotechnol

Ryanodine receptor RyR1-mediated elevation of Ca2+ concentration is required for the late stage of myogenic differentiation and fusion. [Abstract]

2022 Feb 11;13(1):9. PMID: 35144690
Cell Rep

Carvedilol sensitizes chemotherapy by targeting STING to boost anti-tumor immunity. [Abstract]

2025 Apr 16;44(5):115572. PMID: 40249703
J Med Chem

A Novel Compound 3a-M1, from Metabolites of Sinomenine Derivative 3a, Exerts Potent Anti-Aplastic Anemia Activity via IP3R/ORAI-Mediated CTL Ferroptosis. [Abstract]

2025 Apr 17. PMID: 40243551
Front Immunol

Inflammasome-Induced Osmotic Pressure and the Mechanical Mechanisms Underlying Astrocytic Swelling and Membrane Blebbing in Pyroptosis. [Abstract]

2021 Jul 7:12:688674. PMID: 34305921
Biochem Pharmacol

Dual inhibition of EGR1/STAT3 transcriptional hubs suppresses macrophage-driven liver fibrosis: A multi-omics-guided drug repurposing strategy. [Abstract]

2025 Oct:240:117120. PMID: 40623460
Int J Mol Sci

Bufalin Suppresses Pancreatic Ductal Adenocarcinoma Through ER Stress-Ferroptosis Crosstalk Associated with IP3R-Linked Ca2+ Dysregulation and ATF3/SLC7A11 Regulation. [Abstract]

2026 May 14;27(10):4373. PMID: 42196362
Thromb Haemost

Inhibition of IP3 (Inositol 1,4,5-Trisphosphate) Receptors Retards SARS-CoV-2-Induced Endothelial von Willebrand Factor Secretion and Thrombosis. [Abstract]

2025 Mar 6. PMID: 40049586
ACS Omega

Machine Learning for Discovery of GSK3β Inhibitors. [Abstract]

2020 Oct 12;5(41):26551-26561. PMID: 33110983
J Cell Commun Signal

Insights into the mediation of Ca2+ signaling in the promoting effects of LETX-VI on the synthesis and release of dopamine. [Abstract]

2023 Dec;17(4):1309-1321. PMID: 37702818

Dantrolene sodium hemiheptahydrate purchased from MedChemExpress. Usage Cited in: J Cell Commun Signal. 2023 Dec;17(4):1309-1321. [Abstract]

Effect of 2 µM LETX-VI treatment on cytosolic Ca²⁺ level when RyRs in ER membrane was inhibited by Dantrolene (30 µM). The results demonstrated that blockade of RyR by Dantrolene decreased the F340/F380 ratio, indicating that RyR is involved in LETX-VI-induced Ca²⁺ release from the endoplasmic reticulum.
Physiol Rep

Monosodium glutamate-mediated Ca2+-dependent intestinal epithelial ion transports in health and IBS-D in male mice. [Abstract]

2026 Jun;14(11):e70975. PMID: 42281391
Biocell

2026 Jan 23.
bioRxiv

Angiotensin II elicits robust calcium oscillations coordinated within juxtaglomerular cell clusters to suppress renin secretion. [Abstract]

2024 Dec 23:2024.12.23.629519. PMID: 39763801
Res Sq

2024 Jun 11.
Research Square Preprint

2021 May.

Solvent & Solubility

In Vitro:

DMSO : 10 mg/mL (25.04 mM; ultrasonic and warming and adjust pH to 4 with 1 M HCl and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5043 mL

12.5216 mL

25.0432 mL

5 mM

0.5009 mL

2.5043 mL

5.0086 mL

10 mM

0.2504 mL

1.2522 mL

2.5043 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 0.5 mg/mL (1.25 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 0.5 mg/mL (1.25 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zhang W, et al. Neuroprotective effects of dantrolene in neurodegenerative disease: Role of inhibition of pathological inflammation. J Anesth Transl Med. 2024 Jun;3(2):27-35. [Content Brief]

[2]. Choi RH, et al. Dantrolene requires Mg2+ to arrest malignant hyperthermia. Proc Natl Acad Sci U S A. 2017 May 2;114(18):4811-4815. [Content Brief]

[3]. Wang Y, et al. Dantrolene Ameliorates Impaired Neurogenesis and Synaptogenesis in Induced Pluripotent Stem Cell Lines Derived from Patients with Alzheimer's Disease. Anesthesiology. 2020 May;132(5):1062-1079. [Content Brief]

[4]. Zardast M, et al. Evaluation of the Effectiveness of Dantrolene Sodium hemiheptahydrate against Digoxininduced Cardiotoxicity in Adult Rats. Cardiovasc Hematol Agents Med Chem. 2023;22(1):60-65. [Content Brief]

[5]. Osipchuk NC, et al. Modulation of Ryanodine Receptors Activity Alters the Course of Experimental Autoimmune Encephalomyelitis in Mice. Front Physiol. 2021 Dec 17;12:770820. [Content Brief]

[6]. Shi Y, et al. Intranasal Dantrolene as a Disease-Modifying Drug in Alzheimer 5XFAD Mice. J Alzheimers Dis. 2020;76(4):1375-1389. [Content Brief]

[7]. Xu SY, et al. Dantrolene enhances the protective effect of hypothermia on cerebral cortex neurons. Neural Regen Res. 2015 Aug;10(8):1279-85. [Content Brief]

[8]. F Zhao, et al. Dantrolene inhibition of ryanodine receptor Ca2+ release channels. Molecular mechanism and isoform selectivity. J Biol Chem. 2001 Apr 27;276(17):13810-6. [Content Brief]

[9]. Christopher B Sylvester, et al. Dantrolene inhibits lysophosphatidylcholine-induced valve interstitial cell calcific nodule formation via blockade of the ryanodine receptor. Front Cardiovasc Med. 2023 Mar 30:10:1112965. [Content Brief]

[10]. Xi Chen, et al. Dantrolene is neuroprotective in Huntington's disease transgenic mouse model. Mol Neurodegener. 2011 Nov 25;6:81. [Content Brief]

[11]. Büyükokuroğlu ME. Anti-inflammatory and antinociceptive properties of dantrolene sodium hemiheptahydrate in rats and mice. Pharmacol Res. 2002 Jun;45(6):455-60. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5043 mL	12.5216 mL	25.0432 mL	62.6080 mL
	5 mM	0.5009 mL	2.5043 mL	5.0086 mL	12.5216 mL
	10 mM	0.2504 mL	1.2522 mL	2.5043 mL	6.2608 mL
	15 mM	0.1670 mL	0.8348 mL	1.6695 mL	4.1739 mL
	20 mM	0.1252 mL	0.6261 mL	1.2522 mL	3.1304 mL
	25 mM	0.1002 mL	0.5009 mL	1.0017 mL	2.5043 mL

Bioz

Keywords

Dantrolene sodium hemiheptahydrate

17 Cited Publications

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Dantrolene sodium hemiheptahydrate

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