2. Cytoskeleton Anti-infection Membrane Transporter/Ion Channel Apoptosis Stem Cell/Wnt
  3. Exosomes Bacterial Antibiotic Na+/H+ Exchanger (NHE) Parasite Apoptosis Fungal Wnt
  4. Monensin sodium

Monensin sodium  (Synonyms: Monensin A sodium)

Cat. No.: HY-N0150 Purity: 98.0%
COA
SDS
Handling Instructions Technical Support

Monensin (Monensin A) sodium, an orally active antibiotic, is an ionophore that mediates Na+/H+ exchange. Monensin sodium is a potent Wnt signaling inhibitor. Monensin sodium causes a marked enlargement of the multivesicular bodies (MVBs) and regulates exosome secretion. Monensin sodium can be used for bacterial, fungal, and parasitic infections research, and shows anticancer effects.

For research use only. We do not sell to patients.

CAS No. : 22373-78-0

Size Price Stock Quantity
Solution
10 mM * 1 mL in Ethanol In-stock
Solid
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 38 publication(s) in Google Scholar

Other Forms of Monensin sodium:

Monensin sodium Related Antibodies

Top Publications Citing Use of Products

38 Publications Citing Use of MCE Monensin sodium

Cell Migration/Invasion Assay
Cell Imaging/Staining
WB
RT-PCR

    Monensin sodium purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Dec 30;9(1):367.  [Abstract]

    Cellular BiP-IRF2BP2 interactions shown by Western blot analysis of co-immunoprecipitation in MG132-pretreated (5 μM, 0.5 h) Raw264.7 cells which were treated with acidized medium (AcM) or with AcM plus Monensin (0.1 μM) in the presence of LPS and MG132 (0.5 μM) for 6 h.

    Monensin sodium purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Dec 30;9(1):367.  [Abstract]

    Il1b expressions in BMDMs isolated from Hspa5f/f or Hspa5f/f; Lyz2-Cre mice treated with or without Monensin (1 μM), under stimulation of LPS for 24 h.

    Monensin sodium purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 18;16(1):6621.  [Abstract]

    The impact of different concentrations of monensin on the subcellular localization of YFP-ATG8e. 5-day-old YFP-ATG8e transgenic seedlings were treated with different concentrations of Monensin (5-40 μM; 1 h) in 1/2MS liquid medium, followed by confocal microscopy observation.

    Monensin sodium purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 18;16(1):6621.  [Abstract]

    Detection of ROS generation after monensin treatment. 5-day-old mCherry-ATG8f seedlings were subjected to Monensin (20 μM; 1 h) treatment. The ROS-sensitive dye H2DCFDA (1 μM) was incubated 10 min prior to confocal imaging. The images were shown with LUT pseudocolor scale.

    Monensin sodium purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2025 Jul 15;122(28):e2501347122.  [Abstract]

    Boyden chamber transwell assays on mesenchymal NSCLC cells treated with indicated doses of Monensin (100-500 nM; 24 h). Migrated and invaded cells were quantified.

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    Wnt1 Wnt3 Wnt5 Wnt7 Wnt11

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Monensin (Monensin A) sodium, an orally active antibiotic, is an ionophore that mediates Na+/H+ exchange. Monensin sodium is a potent Wnt signaling inhibitor. Monensin sodium causes a marked enlargement of the multivesicular bodies (MVBs) and regulates exosome secretion. Monensin sodium can be used for bacterial, fungal, and parasitic infections research, and shows anticancer effects[1][2][3][4].

    IC50 & Target

    bacterial[1]
    In Vitro

    Monensin (1-5 μM; 48 h) sodium results in a marked decrease in viability in a dose-dependent manner[1].
    Monensin (1-5 μM; 24 h) sodium shows a statistically significant induction of apoptosis[1].
    Monensin (0.1-1 μM; 24 h) sodium inhibits pEGFR and its downstream targets pAKT and pERK[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Monensin sodium Related Antibodies

    Cell Viability Assay[1]

    Cell Line: SCC9, SCC25, and GM-38 cell lines
    Concentration: 1-5 μM
    Incubation Time: 48 h
    Result: Resulted in a marked decrease in viability in a dose-dependent manner.

    Apoptosis Analysis[1]

    Cell Line: SCC25 cells
    Concentration: 1 μM, or 5 μM
    Incubation Time: 24 hours
    Result: Induced a potent apoptotic response.

    Western Blot Analysis[1]

    Cell Line: SCC9 and SCC25 cells
    Concentration: 0.1 μM, or 1 μM
    Incubation Time: 24 hours
    Result: Induced approximately a 50% inhibition of EGF-treated SCC9 cells with respect to pEGFR and its downstream targets pAKT and pERK.
    In Vivo

    Monensin (10 mg/kg; po; daily; for 6 weeks) sodium suppresses progression of the intestinal tumors without any sign of toxicity on normal mucosa in multiple intestinal neoplasia (Min) mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Multiple intestinal neoplasia (Min) mice (four-week-old) [2]
    Dosage: 10 mg/kg
    Administration: Orally application; daily; for 6 weeks
    Result: Suppressed progression of the intestinal tumors without any sign of toxicity on normal mucosa.
    Molecular Weight

    692.85

    Formula

    C36H61NaO11
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OC[C@@]1(O)[C@H](C)C[C@H](C)[C@]([C@@]2(O[C@]([C@@]3(CC[C@@]([C@]4(O[C@@]5(C[C@H](O)[C@@H](C)[C@@]([H])([C@H]([C@@H](OC)[C@H](C)C(O[Na])=O)C)O5)CC4)C)([H])O3)CC)([H])[C@@H](C)C2)[H])([H])O1
    Structure Classification
    Initial Source

    Streptomyces spp
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    Ethanol : 20 mg/mL (28.87 mM; Need ultrasonic)

    DMSO : 2 mg/mL (2.89 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.4433 mL 7.2166 mL 14.4331 mL
    5 mM 0.2887 mL 1.4433 mL 2.8866 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% EtOH    90% Corn Oil

      Solubility: ≥ 2 mg/mL (2.89 mM); Clear solution

      This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.0%

    SDS (480 KB)

    COA (254 KB) HNMR (348 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / Ethanol 1 mM 1.4433 mL 7.2166 mL 14.4331 mL 36.0828 mL
    Ethanol 5 mM 0.2887 mL 1.4433 mL 2.8866 mL 7.2166 mL
    10 mM 0.1443 mL 0.7217 mL 1.4433 mL 3.6083 mL
    15 mM 0.0962 mL 0.4811 mL 0.9622 mL 2.4055 mL
    20 mM 0.0722 mL 0.3608 mL 0.7217 mL 1.8041 mL
    25 mM 0.0577 mL 0.2887 mL 0.5773 mL 1.4433 mL
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    Monensin sodium Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Monensin22373-78-0Monensin AExosomesBacterialAntibioticNa+/H+ Exchanger (NHE)ParasiteApoptosisFungalWntSodium/hydrogen ExchangerSodium-hydrogen ExchangerInhibitorinhibitorinhibit

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