Brilliant Blue R250
Based on 1 publication(s) in Google Scholar
Brilliant blue R250 (Brilliant Blue R), an anionic dye, is the most popular stain to detect proteins resolved in SDS-PAGE gels.
For research use only. We do not sell to patients.
- Purity: 95.0%
- CAS No.: 6104-59-2
- Formula: C45H44N3NaO7S2
- Molecular Weight:825.97
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Storage:
RT, sealed storage, away from moisture and light.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Brilliant Blue R250
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Biological Activity
Guide (The following is our recommended experimental protocol. This protocol is for reference only and should be modified according to your specific needs).
Stain solution preparation:
Add 100 mL of glacial acetic acid to 450 mL ultrapure water.
Dissolve the 3 g of Coomassie Dye in 450 mL methanol.
Filter the solution before use
Standard Gel Staining Protocol
1. Gel may be prefixed in 50% MeOH, 10% HoAC, and 40% H2O for 30 minutes to overnight.
2. Stain gel in the above solution, with 0.25-0.3% Coomassie Blue R-250, for 2-4 hours, until the gel is a uniform blue color. Staining is complete when the gel is no longer visible in the dye solution. Berore complete staining, the gel will appear as a lighter area against the dark staining solution.
3. Destain for 4-24 hours in 5% MeOH, 7.5% HOAC, 87.5% H2O.
4. Store gels in 7% HOAC.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 6104-59-2
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Appearance Solid
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Molecular Weight 825.97
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Formula C45H44N3NaO7S2
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Color Brown to black
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SMILES
O=S(C1=CC(CN(C2=CC=C(/C(C3=CC=C(NC4=CC=C(OCC)C=C4)C=C3)=C5C=C/C(C=C\5)=[N+](CC)\CC6=CC=CC(S(=O)([O-])=O)=C6)C=C2)CC)=CC=C1)(O[Na])=O
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Synonyms
Brilliant Blue R
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
RT, sealed storage, away from moisture and light
In solvent -80°C 1 year -20°C 6 months
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
DMSO : 50 mg/mL (60.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 2 mg/mL (2.42 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.2107 mL | 6.0535 mL | 12.1070 mL | 30.2674 mL |
| DMSO | 5 mM | 0.2421 mL | 1.2107 mL | 2.4214 mL | 6.0535 mL |
| 10 mM | 0.1211 mL | 0.6053 mL | 1.2107 mL | 3.0267 mL | |
| 15 mM | 0.0807 mL | 0.4036 mL | 0.8071 mL | 2.0178 mL | |
| 20 mM | 0.0605 mL | 0.3027 mL | 0.6053 mL | 1.5134 mL | |
| 25 mM | 0.0484 mL | 0.2421 mL | 0.4843 mL | 1.2107 mL | |
| 30 mM | 0.0404 mL | 0.2018 mL | 0.4036 mL | 1.0089 mL | |
| 40 mM | 0.0303 mL | 0.1513 mL | 0.3027 mL | 0.7567 mL | |
| 50 mM | 0.0242 mL | 0.1211 mL | 0.2421 mL | 0.6053 mL | |
| 60 mM | 0.0202 mL | 0.1009 mL | 0.2018 mL | 0.5045 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Brilliant Blue R250 Related Classifications
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