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  4. Brilliant Blue R250

Brilliant Blue R250  (Synonyms: Brilliant Blue R)

Cat. No.: HY-D0232 Purity: ≥95.0%
COA Handling Instructions

Molarity Calculator

Brilliant blue R250 (Brilliant Blue R), an anionic dye, is the most popular stain to detect proteins resolved in SDS-PAGE gels.

For research use only. We do not sell to patients.

Brilliant Blue R250 Chemical Structure

Brilliant Blue R250 Chemical Structure

CAS No. : 6104-59-2

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Based on 1 publication(s) in Google Scholar

Brilliant Blue R250 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Brilliant Blue R250

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Brilliant blue R250 (Brilliant Blue R), an anionic dye, is the most popular stain to detect proteins resolved in SDS-PAGE gels[1].

In Vitro

Stain solution preparation:
Add 100 mL of glacial acetic acid to 450 mL ultrapure water.
Dissolve the 3 g of Coomassie Dye in 450 mL methanol.
Filter the solution before use
Standard Gel Staining Protocol
1. Gel may be prefixed in 50% MeOH, 10% HoAC, and 40% H2O for 30 minutes to overnight.
2. Stain gel in the above solution, with 0.25-0.3% Coomassie Blue R-250, for 2-4 hours, until the gel is a uniform blue color. Staining is complete when the gel is no longer visible in the dye solution. Berore complete staining, the gel will appear as a lighter area against the dark staining solution.
3. Destain for 4-24 hours in 5% MeOH, 7.5% HOAC, 87.5% H2O.
4. Store gels in 7% HOAC.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Brilliant Blue R250 Related Antibodies
Molecular Weight

825.97

Formula

C45H44N3NaO7S2
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

O=S(C1=CC(CN(C2=CC=C(/C(C3=CC=C(NC4=CC=C(OCC)C=C4)C=C3)=C5C=C/C(C=C\5)=[N+](CC)\CC6=CC=CC(S(=O)([O-])=O)=C6)C=C2)CC)=CC=C1)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (60.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 2 mg/mL (2.42 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2107 mL 6.0535 mL 12.1070 mL
5 mM 0.2421 mL 1.2107 mL 2.4214 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1 mg/mL (1.21 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1 mg/mL (1.21 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥95.0%

COA (256 KB) IR (143 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.2107 mL 6.0535 mL 12.1070 mL 30.2674 mL
DMSO 5 mM 0.2421 mL 1.2107 mL 2.4214 mL 6.0535 mL
10 mM 0.1211 mL 0.6053 mL 1.2107 mL 3.0267 mL
15 mM 0.0807 mL 0.4036 mL 0.8071 mL 2.0178 mL
20 mM 0.0605 mL 0.3027 mL 0.6053 mL 1.5134 mL
25 mM 0.0484 mL 0.2421 mL 0.4843 mL 1.2107 mL
30 mM 0.0404 mL 0.2018 mL 0.4036 mL 1.0089 mL
40 mM 0.0303 mL 0.1513 mL 0.3027 mL 0.7567 mL
50 mM 0.0242 mL 0.1211 mL 0.2421 mL 0.6053 mL
60 mM 0.0202 mL 0.1009 mL 0.2018 mL 0.5045 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Brilliant Blue R250 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Brilliant Blue R2506104-59-2Brilliant Blue RBrilliant Blue R 250Brilliant Blue R-250Fluorescent DyeSDS-PAGE gelsanionic dyeprotein stainInhibitorinhibitorinhibit

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