EthD bromide (Synonyms: EtBr; Homidium bromide)

Name: EthD bromide
Brand: MedChemExpress
SKU: HY-D0021
Rating: 5.0 (26 reviews)

Cat. No.: HY-D0021 Purity: 99.27%: Data Sheet
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EthD bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.

For research use only. We do not sell to patients.

CAS No. : 1239-45-8

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	Get quote
1 g	Get quote

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Based on 26 publication(s) in Google Scholar

Other Forms of EthD bromide:

Ethidium bromide (solution) In-stock
EthD-d₅ bromide Get quote

26 Publications Citing Use of MCE EthD bromide

Bio/Physico-chemical Assay

IHC

: EthD bromide purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2025 Feb 13:48:55-70. [Abstract]; EtBr (100 ng/mL) treatment significantly reduced mtDNA copy number.

: EthD bromide purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2025 Feb 13:48:55-70. [Abstract]; WB analysis of proteins in the cGAS-STING pathway after mtDNA depletion (EtBr: 100 ng/mL).

: EthD bromide purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Dec 29;24(1):575.; Rg1 inhibited the opening of hemichannels in astrocytes induced by CORT. (A) Representative images describing EtBr uptake via hemichannels in each group. Red fluorescence indicates EtBr-positive astrocytes. Blue fluorescence indicates nuclei.

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Description

EthD bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	0.1 μM Compound: Ethidium	Concentration required to inhibit 50% of human carcinoma A2780 cell lines Concentration required to inhibit 50% of human carcinoma A2780 cell lines	[PMID: 15501053]
CH1	IC₅₀	0.4 μM Compound: Ethidium	Concentration required to inhibit 50% of human carcinoma CH1 cell lines Concentration required to inhibit 50% of human carcinoma CH1 cell lines	[PMID: 15501053]
HL-60	GI₅₀	5.8 μM Compound: Ethidium bromide	Cytotoxicity against human HL-60 cells assessed as cell growth inhibition measured after 24 hrs by resazurin dye based microplate reader analysis Cytotoxicity against human HL-60 cells assessed as cell growth inhibition measured after 24 hrs by resazurin dye based microplate reader analysis	[PMID: 37988797]
HL-60	GI₅₀	6.4 μM Compound: Ethidium bromide	Cytotoxicity against human HL-60 cells assessed as inhibition of cell growth incubated for 24 hrs and measured at 48 hrs following resazurin challenge by resazurin assay Cytotoxicity against human HL-60 cells assessed as inhibition of cell growth incubated for 24 hrs and measured at 48 hrs following resazurin challenge by resazurin assay	[PMID: 36870272]
MDA-MB-231	IC₅₀	2.33 μM Compound: Ethidium bromide	Inhibition of telomerase in human MDA-MB-231 cells after 24 hrs by TRAP-PCR-ELISA Inhibition of telomerase in human MDA-MB-231 cells after 24 hrs by TRAP-PCR-ELISA	[PMID: 25965778]
MGC-803	IC₅₀	2.1 μM Compound: Ethidium bromide	Inhibition of telomerase in human MGC803 cell extracts after 24 hrs by TRAP-PCR-ELISA Inhibition of telomerase in human MGC803 cell extracts after 24 hrs by TRAP-PCR-ELISA	[PMID: 24119869]
MGC-803	IC₅₀	2.1 μM Compound: Ethidium bromide	Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA	[PMID: 25554922]
MGC-803	IC₅₀	2.18 μM Compound: 2	Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA method Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA method	[PMID: 26883149]
MGC-803	IC₅₀	2.39 μM Compound: Ethidium bromide	Inhibition of telomerase-mediated supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay Inhibition of telomerase-mediated supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay	[PMID: 23265905]
MGC-803	IC₅₀	2.47 μM Compound: Ethidium bromide	Inhibition of telomerase supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay Inhibition of telomerase supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay	[PMID: 22405648]
NIH3T3	IC₅₀	134.87 μM Compound: Ethidium bromide	Cytotoxicity against mouse NIH/3T3 cells after 24 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells after 24 hrs by MTT assay	[PMID: 24286761]
SGC-7901	IC₅₀	2.5 μM Compound: Ethidium bromide	Inhibition of telomerase in human SGC7901 cells by TRAP assay Inhibition of telomerase in human SGC7901 cells by TRAP assay	[PMID: 20800480]
SGC-7901	IC₅₀	2.5 μM Compound: Ethidium bromide	Inhibition of telomerase in human SGC7901 cells by TRAP assay Inhibition of telomerase in human SGC7901 cells by TRAP assay	[PMID: 20947362]
SGC-7901	IC₅₀	2.6 μM Compound: Ethidium bromide	Inhibition of telomerase activity expressed in human SGC7901 cells by SYBER green dye based TRAP Inhibition of telomerase activity expressed in human SGC7901 cells by SYBER green dye based TRAP	[PMID: 21486698]
SGC-7901	IC₅₀	2.6 μM Compound: Ethidium bromide	Inhibition of telomerase in human SGC7901 cells by TRAP assay Inhibition of telomerase in human SGC7901 cells by TRAP assay	[PMID: 23312949]
SK-OV-3	IC₅₀	4.5 μM Compound: Ethidium	Concentration required to inhibit 50% of human carcinoma SKOV3 cell lines Concentration required to inhibit 50% of human carcinoma SKOV3 cell lines	[PMID: 15501053]
SW 1116	IC₅₀	2.71 μM Compound: Ethidium bromide	Inhibition of telomerase in human SW1116 cells after 24 hrs by TRAP-PCR-ELISA assay Inhibition of telomerase in human SW1116 cells after 24 hrs by TRAP-PCR-ELISA assay	[PMID: 24286761]

In Vitro

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
The preparation of agarose gel:
1. Agarose gels are commonly used in concentrations of 0.5% to 2.5% depending on the size of bands needed to be separated.
2. Mix the agarose powder with 1 X TAE/TBE.
3. Microwave for 1-3 min until the agarose is completely dissolved (Caution: not overboil).
4. Make the solution cool down before solidification.
5. Add EtBr to a final concentration of approximately 0.2-0.5 μg/mL.
6. EtBr binds to the DNA and you could visualize the DNA under ultraviolet (UV) light.
(Caution: EtBr is a known mutagen. Please pay attention to strengthening protection.)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

394.31

Formula

C₂₁H₂₀BrN₃

CAS No.

1239-45-8

Appearance

Solid

Color

Dark purple to black

SMILES

CC[N+]1=C(C2=CC=CC=C2)C3=CC(N)=CC=C3C4=C1C=C(N)C=C4.[Br-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (63.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5361 mL	12.6804 mL	25.3608 mL
5 mM	0.5072 mL	2.5361 mL	5.0722 mL
10 mM	0.2536 mL	1.2680 mL	2.5361 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.40%

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Data Sheet (276 KB) SDS (584 KB)

COA (252 KB) HNMR (191 KB) RP-HPLC (184 KB) MS (79 KB)

Handling Instructions (2659 KB)

References

[1]. P Stevenson, et al. Comparison of isometamidium chloride and homidium bromide as prophylactic drugs for trypanosomiasis in cattle at Nguruman, Kenya. Acta Trop. 1995 May;59(2):77-84. [Content Brief]

[2]. Sabnis, Ram Wasudeo (2010). Handbook of Biological Dyes and Stains: Synthesis and Industrial Application. Hoboken, NJ: Wiley. ISBN 978-0-470-40753-0.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5361 mL	12.6804 mL	25.3608 mL	63.4019 mL
	5 mM	0.5072 mL	2.5361 mL	5.0722 mL	12.6804 mL
	10 mM	0.2536 mL	1.2680 mL	2.5361 mL	6.3402 mL
	15 mM	0.1691 mL	0.8454 mL	1.6907 mL	4.2268 mL
	20 mM	0.1268 mL	0.6340 mL	1.2680 mL	3.1701 mL
	25 mM	0.1014 mL	0.5072 mL	1.0144 mL	2.5361 mL
	30 mM	0.0845 mL	0.4227 mL	0.8454 mL	2.1134 mL
	40 mM	0.0634 mL	0.3170 mL	0.6340 mL	1.5850 mL
	50 mM	0.0507 mL	0.2536 mL	0.5072 mL	1.2680 mL
	60 mM	0.0423 mL	0.2113 mL	0.4227 mL	1.0567 mL

EthD bromide Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EthD1239-45-8EtBrHomidiumDNA StainInhibitorinhibitorinhibit

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EthD bromide (Synonyms: EtBr; Homidium bromide)

Customer Review

Other Forms of EthD bromide:

EthD bromide Related Antibodies

26 Publications Citing Use of MCE EthD bromide

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EthD bromide Related Antibodies

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Complete Stock Solution Preparation Table

EthD bromide Related Classifications

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