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Phalloidin 

Cat. No.: HY-P0028 Purity: >96.0%
Handling Instructions

Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome (λex=495 nm, λem=520 nm). Sequence: Ala-{d-Thr}-Cys-{Hyp}-Ala-Trp-Leu (Disulfide bridge: Cys3-Cys6);A-{d-Thr}-C-{Hyp}-AWL (Disulfide bridge: Cys3-Cys6).

For research use only. We do not sell to patients.

Phalloidin Chemical Structure

Phalloidin Chemical Structure

CAS No. : 17466-45-4

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Description

Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome (λex=495 nm, λem=520 nm). Sequence: Ala-{d-Thr}-Cys-{Hyp}-Ala-Trp-Leu (Disulfide bridge: Cys3-Cys6);A-{d-Thr}-C-{Hyp}-AWL (Disulfide bridge: Cys3-Cys6).

In Vitro

Phalloidin staining will not work with methanol-fixed cells, probably because of the disruption of actin filament integrity, but it will work with cells fixed in 0.2% glutaraldehyde in PBS. For fluorescein isothiocyanate (FITC)-Phalloidin (or rhodamine isothiocyanate [RITC]-Phalloidin), the excitation wavelength maximum will be near 495 nm (or 550 nm) and the emission wavelength maximum will be ~520 nm based on H2O at pH 8.0 as the solvent (575 nm with methanol as the solvent). The emission spectrum of fluorescein can depend on the environmental pH and/or ionic strength[1].

Solvent & Solubility
In Vitro: 

H2O

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2676 mL 6.3382 mL 12.6764 mL
5 mM 0.2535 mL 1.2676 mL 2.5353 mL
10 mM 0.1268 mL 0.6338 mL 1.2676 mL
*Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[1]

Prepare a 1:200 dilution of the stock solution (300 units/mL) of fluorescein Phalloidin or rhodamine Phalloidin using PBS. Aspirate the cell medium from the cells grown on glass coverslips and rinse the cells three times with PBS. Fix the cells for 10 min in the 3.7% formaldehyde solution. Rinse the fixed cells three times for 5 min each in PBS. Permeabilize the cells for 5 min in the 0.2% Triton X-100 solution. Rinse the fixed cells three times with PBS. Label the cells with the fluorescein or rhodamine Phalloidin for 5 to 10 min at room temperature. Rinse the cells three times in PBS for 5 min each time. Mount the cell and view the cells using either the fluorescein or rhodamine filter set, depending on the probe[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

788.87

Formula

C₃₅H₄₈N₈O₁₁S

CAS No.

17466-45-4

SMILES

[A-{D-Thr}-C-{Hyp}-AWL(Disulfide bridge: Cys3-Cys6)]

Storage
Powder -80°C 2 years
  -20°C 1 year
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Purity: >96.0%

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Phalloidin
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Phalloidin

Cat. No.: HY-P0028