Phalloidin
Based on 25 publication(s) in Google Scholar
Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome .
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.83%
- No. CAS: 17466-45-4
- Fòrmula: C35H48N8O11S
- Peso molecular:788.87
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Almacenamiento:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Phalloidin
More- Cell. 2025 May 29;188(11):3013-3029.e19. [Abstract]
- Drug Resist Updat. 2024 Mar:73:101056. [Abstract]
- Bone Res. 2025 Feb 14;13(1):23. [Abstract]
- Adv Funct Mater. 2025 Jul 29.
- Adv Compos Hybrid Mater. 2025 Feb 17.
- Adv Sci (Weinh). 2024 Sep 11:e2404571. [Abstract]
- Cell Rep Med. 2026 May 19;7(5):102768. [Abstract]
- Research (Wash D C). 2026 May 7:9:1248. [Abstract]
- Research (Wash D C). 2026 Mar 24.
- Chem Eng J. 2024 Sep 11.
- Cell Commun Signal. 2024 Jan 17;22(1):44. [Abstract]
- Phytomedicine. 2024 Dec:135:156143. [Abstract]
- Adv Healthc Mater. 2025 Jan 22:e2404642. [Abstract]
- J Ethnopharmacol. 2026 Mar 1:358:121033. [Abstract]
- J Mol Cell Biol. 2022 May 20;14(2):mjac001. [Abstract]
- Int Immunopharmacol. 2025 May 30:160:114918. [Abstract]
- ACS Omega. 2025 May 16;10(29):31368-31380. [Abstract]
- Cell Signal. 2024 Jun:118:111147. [Abstract]
- J Cell Mol Med. 2024 Feb;28(4):e18130. [Abstract]
- Poult Sci. 2022 May;101(5):101801. [Abstract]
- Microbiologyopen. 2022 Aug;168(8). [Abstract]
- Cell Reprogram. 2019 Oct;21(5):229-237. [Abstract]
- Int J Ophthalmol. 2025 May 18;18(5):779-791. [Abstract]
- Res Sq. 2024 Jun 20.
- SSRN. 2023 Nov 8.
Actividad biológica
Phalloidin staining will not work with methanol-fixed cells, probably because of the disruption of actin filament integrity, but it will work with cells fixed in 0.2% glutaraldehyde in PBS[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 17466-45-4
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Appearance Solid
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Peso molecular 788.87
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Fòrmula C35H48N8O11S
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Color White to off-white
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Sequence
Ala-{d-Thr}-Cys-{Hyp}-Ala-Trp-Leu (Sulfide bridge: Cys3-Trp6)
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Sequence Shortening
A-{d-Thr}-C-{Hyp}-AWL (Sulfide bridge: Cys3-Trp6)
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (25)
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Journal Impact Factor
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Most Recent
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Cell
2025 May 29;188(11):3013-3029.e19. PMID: 40252640 -
Drug Resist Updat
Drug resistance mechanisms in dopamine agonist-resistant prolactin pituitary neuroendocrine tumors and exploration for new drugs. [Abstract]2024 Mar:73:101056. PMID: 38277755 -
Bone Res
Matrix stiffness regulates nucleus pulposus cell glycolysis by MRTF-A-dependent mechanotransduction. [Abstract]2025 Feb 14;13(1):23. PMID: 39952914 -
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Adv Sci (Weinh)
A Novel Engineering Cell Therapy Platform Mimicking the Immune Thrombocytopenia-Derived Platelets to Inhibit Cytokine Storm in Hemophagocytic Lymphohistiocytosis. [Abstract]2024 Sep 11:e2404571. PMID: 39258712 -
Cell Rep Med
Macrophage-mimetic photothermal nanotherapeutics regulate mitochondrial homeostasis and inflammatory cascades in lung ischemia-reperfusion injury. [Abstract]2026 May 19;7(5):102768. PMID: 42030937 -
Research (Wash D C)
Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells. [Abstract]2026 May 7:9:1248. PMID: 42109884 -
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Cell Commun Signal
Egr-1 is a key regulator of the blood-brain barrier damage induced by meningitic Escherichia coli. [Abstract]2024 Jan 17;22(1):44. PMID: 38233877 -
Phytomedicine
Fangchinoline suppresses hepatocellular carcinoma by regulating ROS accumulation via the TRIM7/Nrf2 signaling pathway. [Abstract]2024 Dec:135:156143. PMID: 39461200 -
Adv Healthc Mater
Bioprinted Fibroblast Mediated Heterogeneous Tumor Microenvironment for Studying Tumor-Stroma Interaction and Drug Screening. [Abstract]2025 Jan 22:e2404642. PMID: 39840601 -
J Ethnopharmacol
Qiang-Xin 1 formula improves cardiac function and alleviates myocardial injury in lipopolysaccharide-induced septic mice by activating calcium/calmodulin-dependent protein kinase I-mediated mitophagy. [Abstract]2026 Mar 1:358:121033. PMID: 41397542 -
J Mol Cell Biol
Inhibition of aryl hydrocarbon receptor signaling promotes the terminal differentiation of human erythroblasts. [Abstract]2022 May 20;14(2):mjac001. PMID: 35022784 -
Int Immunopharmacol
Type I and II pili mediate Streptococcus pneumoniae invasion into human blood-brain barrier-derived cells through extracellular matrix protein receptors and different endocytosis pathways. [Abstract]2025 May 30:160:114918. PMID: 40449266 -
ACS Omega
The Critical Role of Piezo1/CaMKII/β-Catenin Axis in Promoting Osteogenic Differentiation of ADSCs by Pressure Stimulation. [Abstract]2025 May 16;10(29):31368-31380. PMID: 40757337 -
Cell Signal
Unraveling the mechanism in l-Caldesmon regulating the osteogenic differentiation of PDLSCs: An innovative perspective. [Abstract]2024 Jun:118:111147. PMID: 38513808 -
J Cell Mol Med
2024 Feb;28(4):e18130. PMID: 38332511 -
Poult Sci
The protective effect and potential mechanisms of eugenol against Salmonella in vivo and in vitro. [Abstract]2022 May;101(5):101801. PMID: 35338975 -
Microbiologyopen
The inhibitory effect of paeoniflorin on reactive oxygen species alleviates the activation of NF-κB and MAPK signalling pathways in macrophages. [Abstract]2022 Aug;168(8). PMID: 35920812 -
Cell Reprogram
Partially Reprogrammed Induced Pluripotent Stem Cells Using MicroRNA Cluster miR-302s in Guangxi Bama Minipig Fibroblasts. [Abstract]2019 Oct;21(5):229-237. PMID: 31479283 -
Int J Ophthalmol
Nintedanib regulates miR-23b-3p/TGFBR2 axis and competitively binds to TGFBR2 protein, inhibiting EMT process in human pterygium cells. [Abstract]2025 May 18;18(5):779-791. PMID: 40385123 -
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Solvente y solubilidad
DMSO : 100 mg/mL (126.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocolo
Prepare a 1:200 dilution of the stock solution (300 units/mL) of fluorescein Phalloidin or rhodamine Phalloidin using PBS. Aspirate the cell medium from the cells grown on glass coverslips and rinse the cells three times with PBS. Fix the cells for 10 min in the 3.7% formaldehyde solution. Rinse the fixed cells three times for 5 min each in PBS. Permeabilize the cells for 5 min in the 0.2% Triton X-100 solution. Rinse the fixed cells three times with PBS. Label the cells with the fluorescein or rhodamine Phalloidin for 5 to 10 min at room temperature. Rinse the cells three times in PBS for 5 min each time. Mount the cell and view the cells using either the fluorescein or rhodamine filter set, depending on the probe[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureza y Documentación
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Ficha de datos (270 KB)
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SDS (420 KB)
- English - EN (420 KB)
- Français - FR (420 KB)
- Deutsch - DE (420 KB)
- Norwegian - NO (420 KB)
- Español - ES (420 KB)
- Swedish - SV (420 KB)
- Italian - IT (420 KB)
- Korean - KR (420 KB)
- Portuguese - PT (420 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2676 mL | 6.3382 mL | 12.6764 mL | 31.6909 mL |
| 5 mM | 0.2535 mL | 1.2676 mL | 2.5353 mL | 6.3382 mL | |
| 10 mM | 0.1268 mL | 0.6338 mL | 1.2676 mL | 3.1691 mL | |
| 15 mM | 0.0845 mL | 0.4225 mL | 0.8451 mL | 2.1127 mL | |
| 20 mM | 0.0634 mL | 0.3169 mL | 0.6338 mL | 1.5845 mL | |
| 25 mM | 0.0507 mL | 0.2535 mL | 0.5071 mL | 1.2676 mL | |
| 30 mM | 0.0423 mL | 0.2113 mL | 0.4225 mL | 1.0564 mL | |
| 40 mM | 0.0317 mL | 0.1585 mL | 0.3169 mL | 0.7923 mL | |
| 50 mM | 0.0254 mL | 0.1268 mL | 0.2535 mL | 0.6338 mL | |
| 60 mM | 0.0211 mL | 0.1056 mL | 0.2113 mL | 0.5282 mL | |
| 80 mM | 0.0158 mL | 0.0792 mL | 0.1585 mL | 0.3961 mL | |
| 100 mM | 0.0127 mL | 0.0634 mL | 0.1268 mL | 0.3169 mL |
Phalloidin Related Classifications
HY-P0028 Related Classifications
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