18:1 Liss Rhod PE

18:1 Liss Rhod PE is a dedicated fluorescent probe for dip-pen nanolithography (DPN) phospholipid patterning research. 18:1 Liss Rhod PE (1 mol %) exhibits decreased fluorescence intensity in the third bilayer height layer of DPN-patterned DOPC lipid stacks blended with 20 mol % DNP Cap PE, which may result from phase separation-induced local changes in probe concentration^[1].
18:1 Liss Rhod PE is incorporated into phospholipid ink as a fluorescent label to enable large-scale, high-throughput microscopic imaging of stacked phospholipid membrane patterns prepared by DPN^[1].
1. Preparation of Reagents, Equipment and Consumables
Main reagents: 18:1 Liss Rhod PE, carrier phospholipid DOPC, functional phospholipid DNP Cap PE.
Solvent: HPLC-grade chloroform.
Lithography consumables: Inkwell chip, M-2 pen array (both products of NanoInk, Inc.); hydrophilic standard SURF sample (silica oxide substrate with anti-reflective coating, from Nanolane).
Core equipment: DPN 5000 system (NanoInk, Inc.), Nikon Eclipse 80i fluorescence microscope.
2. Preparation of DPN Ink Containing Fluorescent Probe
Preparation of basic stock solution: Prepare a 1 mg/mL DOPC solution using HPLC-grade chloroform as the solvent.
Incorporation of fluorescent probe: Add 18:1 Liss Rhod PE at a fixed molar ratio of 1 mol% to the above DOPC chloroform stock solution, and simultaneously add 0/10/20 mol% of DNP Cap PE according to experimental requirements. Mix thoroughly to obtain DPN lithography ink.
3. Coating of DPN Pen Array and Preparation of Patterns
Ink loading: Add 3 μL of the prepared phospholipid ink into the Inkwell chip.
Pen array coating: Coat the M-2 pen array with the loaded ink under an environment of 70% relative humidity, with a fixed coating duration of 10 min.
Substrate patterning: Fabricate phospholipid patterns on the hydrophilic SURF substrate via the DPN 5000 system under conditions of 48% relative humidity and 24°C room temperature, to obtain stacked lipid membrane structures.
4. Fluorescence Microscopy Monitoring and Quality Control
Imaging screening: Perform fluorescence imaging on the lithographed samples using a Nikon Eclipse 80i fluorescence microscope to obtain information on the overall morphology, size and integrity of lipid patterns, and complete large-area, high-throughput quality screening.
Stability verification: Conduct fluorescence microscopy detection on the same sample both before and after AFM characterization to verify the structural stability of lipid patterns prepared by DPN during the characterization process.
18:1 Liss Rhod PE enables quantitative correlation between the physical height of phospholipid membrane stacks prepared by DPN and the number of membrane layers, achieving rapid characterization of three-dimensional topological information of lipid membrane stacking structures^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.