2. Signaling Pathways
  3. Others
  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D1817
    Vari Fluor 488-Phalloidin
    Vari Fluor 488-Phalloidin is a fluorescent derivative of Phalloidin that specifically labels myofilament proteins and exhibits green fluorescence when labeled, allowing for fluorescence imaging using FITC channels (Ex/Em=488 nm/513 nm).
    Vari Fluor 488-Phalloidin
  • HY-D1617
    BODIPY 500/510 C1, C12
    		99.92%
    BODIPY 500/510 C1, C12 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells. Maximum excitation/emission wavelength: 500/510 nm. Protect from light, stored at -20°C.
    BODIPY 500/510 C1, C12
  • HY-N1389
    Carmine
    Carmine (Carmine red), a natural red dye extracted from the dried females of the insect Dactylopius coccus var. Costa (cochineal). Carmine is a widely used food additive. Carmine provokes both an immediate hypersensitivity and a delayed systemic response with cutaneous expression.
    Carmine
  • HY-P1003
    Ac-DEVD-AMC
    		99.18%
    Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm.
    Ac-DEVD-AMC
  • HY-B1571
    Bromophenol blue
    Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. Bromophenol blue is used as a sensor for the determination of several compounds including ammonia, drugs, proteins and amino acids. The binding of Bromophenol blue to proteins is accompanied by the appearance of a strong absorbance at 610 nm.
    Bromophenol blue
  • HY-15942
    5-TAMRA
    		98.98%
    5-TAMRA can produce bright, pH-insensitive orange-red fluorescence (excitation and emission extremes of 546/579) and has good photostability. 5-TAMRA is mainly used as a fluorescent marker for the synthesis and study of specific oligonucleotide probes.
    5-TAMRA
  • HY-124171
    Zinquin ethyl ester
    		99.0%
    Zinquin ethyl ester is a cell-permeable and lipophilic fluorescent derivative of Zinquin (HY-D0982). Zinquin ethyl ester reacts with protein-bound Zn2+ in cells and forms fluorescent ternary adducts. Zinquin ethyl ester undergoes hydrolysis by intracellular esterases impeding its efflux across the plasma membrane (Ex/Em = 370/470 nm).
    Zinquin ethyl ester
  • HY-D0995
    7-Methyl-6-thioguanosine
    		99.0%
    7-Methyl-6-thioguanosine (MESG) is a chromogenic substrate that can be converted to 7-methyl-6-thioguanine in the presence of purine nucleoside phosphorylase and inorganic phosphate. 7-Methyl-6-thioguanosine can be used to quantify inorganic phosphate. 7-Methyl-6-thioguanosine can also be used to determine the activities of purine nucleoside phosphorylase and protein phosphatases.
    7-Methyl-6-thioguanosine
  • HY-W127703
    Octadecyl Rhodamine B chloride
    		99.58%
    Octadecyl Rhodamine B chloride is an amphiphilic fluorescent probe with both membrane-binding ability and fluorescent properties, which easily forms aggregates in aqueous phase leading to self-quenching. Octadecyl Rhodamine B chloride can be used for cell membrane staining and further applied in membrane biology research. The maximum absorption/emission wavelengths of Octadecyl Rhodamine B chloride monomer in Triton X-100 are 565 nm/585 nm; the absorption peaks of aggregates in water are blue-shifted to 524 nm and 574 nm, with significantly quenched fluorescence.
    Octadecyl Rhodamine B chloride
  • HY-140947
    TAMRA-Azide-PEG-biotin
    		99.0%
    TAMRA-Azide-PEG-biotin is a dye derivative of TAMRA (HY-135640). TAMRA-Azide-PEG-biotin contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    TAMRA-Azide-PEG-biotin
  • HY-D1696
    MitoTracker Orange CMTMRos
    MitoTracker Orange CMTMRos is a fluorescent dye that labels mitochondria within live cells utilizing the mitochondrial membrane potential (Ex/Em: 551/576 nm).
    MitoTracker Orange CMTMRos
  • HY-P0019A
    Z-Gly-Gly-Arg-AMC acetate
    		99.84%
    Z-Gly-Gly-Arg-AMC acetate is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP) (Ex/Em = 390/480 nm).
    Z-Gly-Gly-Arg-AMC acetate
  • HY-P1005
    Ac-DEVD-AFC
    		98.89%
    Ac-DEVD-AFC is a fluorogenic substrate (λex=400 nm, λem=530 nm).
    Ac-DEVD-AFC
  • HY-D0715
    Fluorescamine
    		98.0%
    Fluorescamine is a spirocyclic compound that is non-fluorescent. Fluorescamine reacts rapidly with primary amine groups in proteins under alkaline conditions to generate products with strong fluorescence (Ex/Em: 390/475 nm). Fluorescamine can be used to detect amine-containing compounds, including amino acids, peptides, and proteins.
    Fluorescamine
  • HY-D1836
    Vari Fluor 555 TSA (200x)
    Vari Fluor 555 TSA (200x) , also know as VF 555 Tyramide. Vari Fluor 555 TSA (200x) is one of Vari Fluor TSA series fluorescent probes, which can be used for high-density in situ labeling assays. Vari Fluor TSA series target antigens via horseradish peroxidase (HRP), which are widely used in ELISA, IF, IHC and other techniques with high detection sensitivity.
    Vari Fluor 555 TSA (200x)
  • HY-D1563
    pH Receptor Probe
    		98.40%
    pH Receptor Probe is a pH-sensitive cyanine derivative and a environmental sensitive fluorophore. pH Receptor Probe has a pH-sensitive maximal absorption at 644 nm and emission at 664 nm. pH Receptor Probe has been used as a pH sensor of lysosomal compartments.
    pH Receptor Probe
  • HY-110250
    DFHBI
    		99.13%
    DFHBI is a small molecule that resembles the chromophore of green fluorescent protein (GFP). DFHBI can be used for imaging RNA in living cells. . Spinach and DFHBI are essentially nonfluorescent when unbound, whereas the Spinach-DFHBI complex is brightly fluorescent both in vitro and in living cells (Ex/Em = 469/501 nm).
    DFHBI
  • HY-15944
    5(6)-TAMRA
    		99.42%
    5(6)-TAMRA (5(6)-Carboxytetramethylrhodamine) is a fluorescent dye molecule widely used as a label for peptides and proteins (Ex/Em = 520/600 nm).
    5(6)-TAMRA
  • HY-D1389
    Sulfo-CY-5.5 NHS ester tripotassium
    Sulfo-CY-5.5 NHS ester (Sulfo-Cyanine5.5 NHS ester) tripotassium is an amine-reactive ester of sulfonated far-red Cyanine5.5 fluorophore, which can be used to label antibodies, proteins, etc., as well as for in vivo NIR imaging.
    Sulfo-CY-5.5 NHS ester tripotassium
  • HY-NP163A
    Wheat germ agglutinin-AF488
    Wheat germ agglutinin-AF488 (WGA-AF488) is a cell membrane-specific staining agent prepared by conjugating wheat germ agglutinin with the Alexa Fluor 488 (HY-D1304) fluorescent dye, and it binds to cell surface glycoproteins with high affinity. Wheat germ agglutinin-AF488 is applied in fluorescence microscopy and confocal imaging techniques, and it can clearly label the membrane structures of various cells including breast cancer cells, enabling high-resolution visual observation. Wheat germ agglutinin-AF488 is used in studies of breast cancer and triple-negative breast cancer to observe cell morphology and membrane dynamic changes.
    Wheat germ agglutinin-AF488
Cat. No. Product Name / Synonyms Application Reactivity