Bromophenol blue

Name: Bromophenol blue
Brand: MedChemExpress
SKU: HY-B1571
Rating: 4.5 (1 reviews)

Cat. No.: HY-B1571: Data Sheet
COA

SDS
Handling Instructions
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Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. Bromophenol blue is used as a sensor for the determination of several compounds including ammonia, drugs, proteins and amino acids. The binding of Bromophenol blue to proteins is accompanied by the appearance of a strong absorbance at 610 nm.

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Bromophenol blue Chemical Structure

CAS No. : 115-39-9

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	Get quote
50 g	Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Bromophenol blue:

Bromophenol blue sodium In-stock

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of Bromophenol blue Staining Solution:^[2]
Prepare Bromophenol blue staining solution with DMSO.
2. Protein assay:^[2]
2.1 Proteins were dissolved in distilled water, and their concentrations were adjusted to 1 mg/mL with the exceptions of the myoglobin solution which was prepared gravimetrically, and the y-globulin solution, which was made according to the suppliers.
2.2 For performing the protein assay, protein solutions containing from 10 to 80 μg of protein in 0.1 mL were mixed with 0.9 mL of the Bromophenol blue solution.
2.3 The absorbance at 610 nm was determined using a blank made by mixing 0.1 mL of distilled water and 0.9 mL of the dye solution.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

669.96

Formula

C₁₉H₁₀Br₄O₅S

CAS No.

115-39-9

Appearance

Solid

Color

Orange to red

SMILES

O=S1(OC(C2=CC(Br)=C(O)C(Br)=C2)(C3=CC(Br)=C(O)C(Br)=C3)C4=CC=CC=C41)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, stored under nitrogen

In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (74.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4926 mL	7.4631 mL	14.9263 mL
5 mM	0.2985 mL	1.4926 mL	2.9853 mL
10 mM	0.1493 mL	0.7463 mL	1.4926 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

Purity & Documentation

Select Batch:

Data Sheet (282 KB) SDS (393 KB)

COA (262 KB)

Handling Instructions (2659 KB)

References

[1]. Dangui AZ, et, al. Preferential solvation bromophenol blue in water-alcohol binary mixture. Spectrochim Acta A Mol Biomol Spectrosc. 2018 Oct 5; 203: 333-341. [Content Brief]

[2]. Flores R. A rapid and reproducible assay for quantitative estimation of proteins using bromophenol blue. Anal Biochem. 1978 Aug 1;88(2):605-11. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.4926 mL	7.4631 mL	14.9263 mL	37.3157 mL
	5 mM	0.2985 mL	1.4926 mL	2.9853 mL	7.4631 mL
	10 mM	0.1493 mL	0.7463 mL	1.4926 mL	3.7316 mL
	15 mM	0.0995 mL	0.4975 mL	0.9951 mL	2.4877 mL
	20 mM	0.0746 mL	0.3732 mL	0.7463 mL	1.8658 mL
	25 mM	0.0597 mL	0.2985 mL	0.5971 mL	1.4926 mL
	30 mM	0.0498 mL	0.2488 mL	0.4975 mL	1.2439 mL
	40 mM	0.0373 mL	0.1866 mL	0.3732 mL	0.9329 mL
	50 mM	0.0299 mL	0.1493 mL	0.2985 mL	0.7463 mL
	60 mM	0.0249 mL	0.1244 mL	0.2488 mL	0.6219 mL