115-39-9
Chemical Structure
Bromophenol blue
- CAS No.: 115-39-9
- Formula:C19H10Br4O5S
- Molecular Weight:669.96
IUPAC Name: 3,3-bis(3,5-dibromo-4-hydroxyphenyl)-3H-benzo[c][1,2]oxathiole 1,1-dioxide
InChIKey: UDSAIICHUKSCKT-UHFFFAOYSA-N
SMILES: O=S1(OC(C2=CC(Br)=C(O)C(Br)=C2)(C3=CC(Br)=C(O)C(Br)=C3)C4=CC=CC=C41)=O
Biological Activity: Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. Bromophenol blue is used as a sensor for the determination of several compounds including ammonia, drugs, proteins and amino acids. The binding of Bromophenol blue to proteins is accompanied by the appearance of a strong absorbance at 610 nm[1][2].
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Bromophenol blue | Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. Bromophenol blue is used as a sensor for the determination of several compounds including ammonia, drugs, proteins and amino acids. The binding of Bromophenol blue to proteins is accompanied by the appearance of a strong absorbance at 610 nm. | |||||||||||||||||||||
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Bromophenol blue indicator (3.0-4.6) | Bromophenol blue indicator (3.0-4.6) is a synthetic dye commonly used as an acid-base indicator with a transition range of pH 3.0-4.6. Bromophenol blue indicator (3.0-4.6) is water soluble and changes color from yellow to blue as the pH of the solution changes from acidic to basic. Its unique chemical properties make it an important ingredient in a variety of scientific applications, especially in biochemistry and molecular biology. In addition, it can be used as a stain in microbiology and histology. However, Bromophenol blue indicator (3.0-4.6) has potential irritating and staining properties. | |||||||||||||||||||||
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References
- [1]. Dangui AZ, et, al. Preferential solvation bromophenol blue in water-alcohol binary mixture. Spectrochim Acta A Mol Biomol Spectrosc. 2018 Oct 5; 203: 333-341. [Content Brief]
- [2]. Flores R. A rapid and reproducible assay for quantitative estimation of proteins using bromophenol blue. Anal Biochem. 1978 Aug 1;88(2):605-11. [Content Brief]
