Bromophenol blue indicator (3.0-4.6)

Name: Bromophenol blue indicator (3.0-4.6)
Brand: MedChemExpress
SKU: HY-W110798
Rating: 4.5 (1 reviews)

Cat. No.: HY-W110798: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Bromophenol blue indicator (3.0-4.6) is a synthetic dye commonly used as an acid-base indicator with a transition range of pH 3.0-4.6. Bromophenol blue indicator (3.0-4.6) is water soluble and changes color from yellow to blue as the pH of the solution changes from acidic to basic. Its unique chemical properties make it an important ingredient in a variety of scientific applications, especially in biochemistry and molecular biology. In addition, it can be used as a stain in microbiology and histology. However, Bromophenol blue indicator (3.0-4.6) has potential irritating and staining properties.

For research use only. We do not sell to patients.

Bromophenol blue indicator (3.0-4.6) Chemical Structure

CAS No. : 115-39-9

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
10 g	USD 25	In-stock	Estimated Time of Arrival: December 31
25 g	USD 45	In-stock	Estimated Time of Arrival: December 31
50 g		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
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Description

In Vitro

Bromophenol blue indicator (3.0-4.6) is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

669.96

Formula

C₁₉H₁₀Br₄O₅S

CAS No.

115-39-9

Appearance

Solid

SMILES

O=S1(OC(C2=CC(Br)=C(O)C(Br)=C2)(C3=CC(Br)=C(O)C(Br)=C3)C4=CC=CC=C41)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (149.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4926 mL	7.4631 mL	14.9263 mL
5 mM	0.2985 mL	1.4926 mL	2.9853 mL
10 mM	0.1493 mL	0.7463 mL	1.4926 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Select Batch:

Data Sheet (256 KB) SDS (393 KB)

COA (264 KB) HNMR (116 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.4926 mL	7.4631 mL	14.9263 mL	37.3157 mL
	5 mM	0.2985 mL	1.4926 mL	2.9853 mL	7.4631 mL
	10 mM	0.1493 mL	0.7463 mL	1.4926 mL	3.7316 mL
	15 mM	0.0995 mL	0.4975 mL	0.9951 mL	2.4877 mL
	20 mM	0.0746 mL	0.3732 mL	0.7463 mL	1.8658 mL
	25 mM	0.0597 mL	0.2985 mL	0.5971 mL	1.4926 mL
	30 mM	0.0498 mL	0.2488 mL	0.4975 mL	1.2439 mL
	40 mM	0.0373 mL	0.1866 mL	0.3732 mL	0.9329 mL
	50 mM	0.0299 mL	0.1493 mL	0.2985 mL	0.7463 mL
	60 mM	0.0249 mL	0.1244 mL	0.2488 mL	0.6219 mL
	80 mM	0.0187 mL	0.0933 mL	0.1866 mL	0.4664 mL
	100 mM	0.0149 mL	0.0746 mL	0.1493 mL	0.3732 mL