Bromocresol purple sodium 

Bromocresol purple sodium is a pH indicator, whose color changes from yellow to purple when the pH increases from 5.2 to 6.8. Bromocresol purple sodium can be used for detecting yeast cells with plasma membrane damage and measuring serum albumin concentrations^[1][2][3].

Arabidopsis thaliana (L.) seedlings are transferred to Murashige and Skoog (MS) or low phosphorus medium which contained 0.75% [weight/volume (w/v)] agar, 0.006% (w/v) Bromocresol purple sodium, pH 5.8 for 8-24 h. There are no significant color changes around the roots of wild-type (WT) seedlings on the MS medium^[1].
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. The yeast cells were centrifuged, washed with distilled water and resuspended in 5mM-citrate-phosphate buffer, pH 4.6.
2. Aliquots containing 10⁷ cells were suspended in the same buffer, supplemented with glucose to a final concentration of 220 mM and different volumes of killer toxin preparation were added so that the final volume was 1 mL.
3. Incubation with the killer was then carried out in Eppendorf vials at 25°C on a shaker for 120 min.
4. 50 μL 10 mM Bromocresol purple sodium in 5 mM-citrate-phosphate buffer, pH 4.6, was added to the samples and incubation continued for 60 min under the same conditions.
5. The samples were then centrifuged, thoroughly washed with 5 mM-citrate-phosphate buffer.
6. The fraction of stained cells was determined in a-Jenalumar fluorescence microscope using violet light for excitation. Cell fluorescence was observed using filter G 251, which cuts off wavelengths below 450 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

562.20

C₂₁H₁₅Br₂NaO₅S

62625-30-3

Solid

Brown to black

O=S1(OC(C2=CC(Br)=C(C(C)=C2)O[Na])(C3=C1C=CC=C3)C4=CC(Br)=C(C(C)=C4)O)=O

Room temperature in continental US; may vary elsewhere.

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Lei K J, et al. Screening and analysis of rhizosphere acidification deficiency mutants in Arabidopsis thaliana under low phosphorus[J].Soil ence and Plant Nutrition, 2015, 61(3):1-8.

  [2]. Kurzweilová H, et al. Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity. Yeast. 1993 Nov;9(11):1207-11.  [Content Brief]

  [3]. Affonso A, et al. Bromocresol purple dye-binding method for the estimation of serum albumin adapted to the SMA 12/60. Clin Biochem. 1985 Oct;18(5):285-9.  [Content Brief]