Bromocresol purple

Arabidopsis thaliana (L.) seedlings are transferred to Murashige and Skoog (MS) or low phosphorus medium which contained 0.75% [weight/volume (w/v)] agar, 0.006% (w/v) Bromocresol purple, pH 5.8 for 8-24 h. There are no significant color changes around the roots of wild-type (WT) seedlings on the MS medium^[1].
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. The yeast cells were centrifuged, washed with distilled water and resuspended in 5mM-citrate-phosphate buffer, pH 4.6.
2. Aliquots containing 10⁷ cells were suspended in the same buffer, supplemented with glucose to a final concentration of 220 mM and different volumes of killer toxin preparation were added so that the final volume was 1 mL.
3. Incubation with the killer was then carried out in Eppendorf vials at 25°C on a shaker for 120 min.
4. 50 μL 10 mM Bromocresol purple in 5 mM-citrate-phosphate buffer, pH 4.6, was added to the samples and incubation continued for 60 min under the same conditions.
5. The samples were then centrifuged, thoroughly washed with 5 mM-citrate-phosphate buffer.
6. The fraction of stained cells was determined in a-Jenalumar fluorescence microscope using violet light for excitation. Cell fluorescence was observed using filter G 251, which cuts off wavelengths below 450 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.