Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity

  • Yeast. 1993 Nov;9(11):1207-11. doi: 10.1002/yea.320091107.
H Kurzweilová  1 ,  K Sigler
Affiliations
  • 1. Institute of Microbiology, Czech Academy of Sciences, Prague.
PMID: 7509098 DOI: 10.1002/yea.320091107
Abstract

A method is described for detecting yeast cells with plasma membrane damage, based on cell staining with bromocresol purple (BCP) which has a convenient fluorescence after entering the cells at pH below 5.2. The method was used to determine the activity of Saccharomyces cerevisiae pore-forming killer toxin K1 in commonly used lethal units. The BCP test is rapid and as precise as the plating test.

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