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  4. MitoTracker Orange CMTMRos

MitoTracker Orange CMTMRos is a fluorescent dye that labels mitochondria within live cells utilizing the mitochondrial membrane potential (Ex/Em: 551/576 nm).

For research use only. We do not sell to patients.

MitoTracker Orange CMTMRos Chemical Structure

MitoTracker Orange CMTMRos Chemical Structure

CAS No. : 199116-50-2

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1 mg USD 280 In-stock
5 mg USD 980 In-stock
10 mg USD 1650 In-stock
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Based on 1 publication(s) in Google Scholar

MitoTracker Orange CMTMRos Related Antibodies

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Description

MitoTracker Orange CMTMRos is a fluorescent dye that labels mitochondria within live cells utilizing the mitochondrial membrane potential (Ex/Em: 551/576 nm)[1].

IC50 & Target

Mitochondria[1]
In Vitro

Guidelines[1] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Cells:
For live cells, follow Steps 1–7. For fixed cells, follow Steps 8–13.
1. Prepare a labeling solution at 25–500 nM in the desired cell culture medium, and warm it to the cell culture temperature.
2. Aspirate the culture medium from cells grown on coverslips.
3. Immerse the cells in the labeling medium for 15–45 min at 37?C.
4. Aspirate the labeling medium, and rinse the cells three times with culture medium.
5. Mount the cells.
6. Check the cells using a fluorescence microscope. If the fluorescence staining is too low, try one of the following options:
i. Incubate for an additional 30 min in the normal culture medium to allow the thiol conjugation to proceed.
ii. Try a higher initial concentration of the labeling solution.
7. When the fluorescence is sufficient, image the cells.
8. Carry out Steps 1–4, but use a labeling solution at a working concentration between 100 and 1000 nM.
9. Rinse the cells three times with PBS+.
10. Fix the cells for 10 min in 3.7% formaldehyde in PBS (pH 7.4).
11. Aspirate and rinse the cells three times for 5 min each in PBS.
12. Mount the coverslips.
13. Image the cells.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MitoTracker Orange CMTMRos Related Antibodies
Molecular Weight

427.37

Formula

C24H24Cl2N2O
CAS No.
Appearance

Solid

Color

Brown to black

Emission (Em)

576
Excitation (Ex)

554
SMILES

CN(C)C(C=C1)=CC2=C1C(C3=CC=C(CCl)C=C3)=C(C=C/4)C(O2)=CC4=[N+](C)/C.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 31.25 mg/mL (73.12 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3399 mL 11.6995 mL 23.3989 mL
5 mM 0.4680 mL 2.3399 mL 4.6798 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3399 mL 11.6995 mL 23.3989 mL 58.4973 mL
5 mM 0.4680 mL 2.3399 mL 4.6798 mL 11.6995 mL
10 mM 0.2340 mL 1.1699 mL 2.3399 mL 5.8497 mL
15 mM 0.1560 mL 0.7800 mL 1.5599 mL 3.8998 mL
20 mM 0.1170 mL 0.5850 mL 1.1699 mL 2.9249 mL
25 mM 0.0936 mL 0.4680 mL 0.9360 mL 2.3399 mL
30 mM 0.0780 mL 0.3900 mL 0.7800 mL 1.9499 mL
40 mM 0.0585 mL 0.2925 mL 0.5850 mL 1.4624 mL
50 mM 0.0468 mL 0.2340 mL 0.4680 mL 1.1699 mL
60 mM 0.0390 mL 0.1950 mL 0.3900 mL 0.9750 mL
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MitoTracker Orange CMTMRos Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MitoTracker Orange CMTMRos199116-50-2Fluorescent Dyefluorescent dyemitochondriaInhibitorinhibitorinhibit

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