BODIPY 500/510 C1, C12
Based on 2 publication(s) in Google Scholar
BODIPY 500/510 C1, C12 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells. Maximum excitation/emission wavelength: 500/510 nm. Protect from light, stored at -20°C.
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 144672-74-2
- Formula: C22H31BF2N2O2
- Molecular Weight:404.30
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) BODIPY 500/510 C1, C12
More
Biological Activity
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of BODIPY 500/510 C1, C12 working solution
1.1 Preparation of the stock solution
Dissolve BODIPY 500/510 C1, C12 in DMSO to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20°C or -80°C, keep away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of BODIPY 500/510 C1, C12 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of BODIPY 500/510 C1, C12 working solution according to the actual situation.
2. Cell staining (Suspension cells)
2.1 Centrifuge at 1000 g at 4°C for 3-5 min and then discard the supernatant. Wash twice with PBS, 5 min each time. The cell density is 1×106/mL.
2.2 Add 1 mL of working solution, and then incubate at room temperature for 5-30 min.
2.3 Centrifuge at 400 g at 4°C for 3-4 min and then discard the supernatant.
2.4 Wash twice with PBS, 5 min each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
3. Cell staining (Adherent cells)
3.1 Culture adherent cells on sterile coverslips.
3.2 Remove the coverslip from the medium and aspirate excess medium.
3.3 Add 100 μL of working solution, gently shake it to completely cover the cells, and then incubate at room temperature for 5-30 min.
3.4 Wash twice with medium, 5 min each time. Observation by fluorescence microscopy or flow cytometry.
Precautions
1. Please adjust the concentration of BODIPY 500/510 C1, C12 working solution according to the actual situation.
2. Experiments suggest a positive control, incubate the control group cells with 30 μM oleic acid for 8 h and then perform subsequent experiments.
3. This product is only for R&D use, not for drug, household, or others.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
510
500
Chemical Information
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CAS No. 144672-74-2
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Appearance Solid
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Molecular Weight 404.30
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Formula C22H31BF2N2O2
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Color Orange to red
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SMILES
[F-][B+3]1([N-]2C(C=C(C=C3)[N]1=C3C)=CC=C2CCCCCCCCCCCC(O)=O)[F-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Nat Commun
Commitment of adipose-resident c-kit+ progenitors to brown adipocytes contributes to adipose tissue homeostasis and remodeling. [Abstract]2025 Jul 1;16(1):5883. PMID: 40593548 -
Pharmacol Res
2026 Feb:224:108085. PMID: 41485679
Solvent & Solubility
DMSO : 100 mg/mL (247.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Lyu Z, et al. PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules. EBioMedicine. 2018 Dec;38:178-190. [Content Brief]
[2]. Kamkaew A, et al. BODIPY dyes in photodynamic therapy. Chem Soc Rev. 2013 Jan 7;42(1):77-88. [Content Brief]
[3]. Bo Qiu, et al. BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. Bio Protoc. 2016 Sep 5;6(17):e1912. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4734 mL | 12.3671 mL | 24.7341 mL | 61.8353 mL |
| 5 mM | 0.4947 mL | 2.4734 mL | 4.9468 mL | 12.3671 mL | |
| 10 mM | 0.2473 mL | 1.2367 mL | 2.4734 mL | 6.1835 mL | |
| 15 mM | 0.1649 mL | 0.8245 mL | 1.6489 mL | 4.1224 mL | |
| 20 mM | 0.1237 mL | 0.6184 mL | 1.2367 mL | 3.0918 mL | |
| 25 mM | 0.0989 mL | 0.4947 mL | 0.9894 mL | 2.4734 mL | |
| 30 mM | 0.0824 mL | 0.4122 mL | 0.8245 mL | 2.0612 mL | |
| 40 mM | 0.0618 mL | 0.3092 mL | 0.6184 mL | 1.5459 mL | |
| 50 mM | 0.0495 mL | 0.2473 mL | 0.4947 mL | 1.2367 mL | |
| 60 mM | 0.0412 mL | 0.2061 mL | 0.4122 mL | 1.0306 mL | |
| 80 mM | 0.0309 mL | 0.1546 mL | 0.3092 mL | 0.7729 mL | |
| 100 mM | 0.0247 mL | 0.1237 mL | 0.2473 mL | 0.6184 mL |
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