BODIPY FL C12
Based on 1 Customer Validation
BODIPYFL C12 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells. Maximum excitation/emission wavelength: 480/508 nm.
For research use only. We do not sell to patients.
- Purity: 99.79%
- CAS No.: 158757-79-0
- Formula: C23H33BF2N2O2
- Molecular Weight:418.33
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of BODIPY FL C12 working solution
1.1 Preparation of the stock solution
Dissolve BODIPY FL C12 in DMSO to obtain 10 mM of BODIPY FL C12.
Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of BODIPY FL C12 working solution
Dilute the stock solution in HBSS to obtain 1-10 μM of BODIPY FL C12 working solution.
Note: Please adjust the concentration of BODIPY FL C12 working solution according to the actual situation.
2. Cell staining (Suspension cells)
2.1 Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×106mL.
2.2 Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
3. Cell staining (Adherent cells)
3.1 Culture adherent cells on sterile coverslips.
3.2 Remove the coverslip from the medium and aspirate excess medium.
3.3 Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
3.4 Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.
Precautions
1. It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles.
2. Please adjust the concentration of BODIPY FL C12 working solution according to the actual situation.
3. when the cell culture solution is removed with poor results, the cells can be washed with an appropriate amount of Hanks balanced salt solution.
4. This product is for R&D use only, not for drug, household, or other uses.
5. For your safety and health, please wear a lab coat and disposable gloves to operate.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
516
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Chemical Information
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CAS No. 158757-79-0
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Appearance Solid
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Molecular Weight 418.33
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Formula C23H33BF2N2O2
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Color Orange to red
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SMILES
CC1=C2C=C3C=CC(CCCCCCCCCCCC(O)=O)=[N-]3[B+3]([F-])(N2C(C)=C1)[F-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 50 mg/mL (119.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Kang ZB, et al. Fluopack screening platform for unbiased cellular phenotype profiling. Sci Rep. 2020 Feb 7;10(1):2097. [Content Brief]
[2]. Kamkaew A, et al. BODIPY dyes in photodynamic therapy. Chem Soc Rev. 2013 Jan 7;42(1):77-88. [Content Brief]
[3]. Bo Qiu, et al. BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. Bio Protoc. 2016 Sep 5;6(17):e1912. [Content Brief]
[4]. Glende J, et al. Importance of cholesterol-rich membrane microdomains in the interaction of the S protein of SARS-coronavirus with the cellular receptor angiotensin-converting enzyme 2. Virology. 2008 Nov 25;381(2):215-21. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3905 mL | 11.9523 mL | 23.9046 mL | 59.7614 mL |
| 5 mM | 0.4781 mL | 2.3905 mL | 4.7809 mL | 11.9523 mL | |
| 10 mM | 0.2390 mL | 1.1952 mL | 2.3905 mL | 5.9761 mL | |
| 15 mM | 0.1594 mL | 0.7968 mL | 1.5936 mL | 3.9841 mL | |
| 20 mM | 0.1195 mL | 0.5976 mL | 1.1952 mL | 2.9881 mL | |
| 25 mM | 0.0956 mL | 0.4781 mL | 0.9562 mL | 2.3905 mL | |
| 30 mM | 0.0797 mL | 0.3984 mL | 0.7968 mL | 1.9920 mL | |
| 40 mM | 0.0598 mL | 0.2988 mL | 0.5976 mL | 1.4940 mL | |
| 50 mM | 0.0478 mL | 0.2390 mL | 0.4781 mL | 1.1952 mL | |
| 60 mM | 0.0398 mL | 0.1992 mL | 0.3984 mL | 0.9960 mL | |
| 80 mM | 0.0299 mL | 0.1494 mL | 0.2988 mL | 0.7470 mL | |
| 100 mM | 0.0239 mL | 0.1195 mL | 0.2390 mL | 0.5976 mL |
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