BODIPY FL C5-Ceramide
Based on 1 Customer Validation
The Golgi apparatus is composed of flattened vesicles superimposed on each other by unit membranes. The flattened vesicles are round with expanded and perforated edges. The Golgi fluorescent probe is a BODIPY-labeled ceramide derivative, the synthesis of which occurs in the endoplasmic reticulum and can then be transported to the Golgi via ceramide transport protein (CERT) or vesicular translocation, allowing specific labeling of the dye. BODIPY Fl C5-Ceramide is a Golgi-specific green fluorescent dye, which can visualise individual cells. Ex/Em= 505 nm/512 nm.
For research use only. We do not sell to patients.
- Purity: 98.87%
- CAS No.: 133867-53-5
- Formula: C34H54BF2N3O3
- Molecular Weight:601.62
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of BODIPY FL C5-Ceramide working solution
1.1 Preparation of the stock solution
Dissolve BODIPY FL C5-Ceramide in DMSO to obtain 5 mM of BODIPY FL C5-Ceramide.
Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of BODIPY FL C5-Ceramide working solution
Dilute the stock solution in HBSS to obtain 1-10 μM of BODIPY FL C5-Ceramide working solution.
Note: Please adjust the concentration of BODIPY FL C5-Ceramide working solution according to the actual situation.
2. Cell staining (Suspension cells)
2.1 Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×106mL.
2.2 Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
3. Cell staining (Adherent cells)
3.1 Culture adherent cells on sterile coverslips.
3.2 Remove the coverslip from the medium and aspirate excess medium.
3.3 Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
3.4 Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.
Precautions
1. Please adjust the concentration of BODIPY FL C5-Ceramide working solution according to the actual situation.
2. when the cell culture solution is removed with poor results, the cells can be washed with an appropriate amount of Hanks balanced salt solution.
3. This product is for R&D use only, not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
511
505
Chemical Information
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CAS No. 133867-53-5
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Appearance Solid
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Molecular Weight 601.62
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Formula C34H54BF2N3O3
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Color Brown to red
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SMILES
O=C(CCCCC1=CC=C2C=C3C(C)=CC(C)=[N]3[B+3]([F-])([N-]21)[F-])N[C@H]([C@@H](/C=C/CCCCCCCCCCCCC)O)CO
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 50 mg/mL (83.11 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Alamudi SH, et al. Development of background-free tame fluorescent probes for intracellular live cell imaging. Nat Commun. 2016 Jun 20;7:11964. [Content Brief]
[2]. Podgorny OV, et al. Isolation of single Chlamydia-infected cells using laser microdissection. J Microbiol Methods. 2015 Feb;109:123-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6622 mL | 8.3109 mL | 16.6218 mL | 41.5545 mL |
| 5 mM | 0.3324 mL | 1.6622 mL | 3.3244 mL | 8.3109 mL | |
| 10 mM | 0.1662 mL | 0.8311 mL | 1.6622 mL | 4.1554 mL | |
| 15 mM | 0.1108 mL | 0.5541 mL | 1.1081 mL | 2.7703 mL | |
| 20 mM | 0.0831 mL | 0.4155 mL | 0.8311 mL | 2.0777 mL | |
| 25 mM | 0.0665 mL | 0.3324 mL | 0.6649 mL | 1.6622 mL | |
| 30 mM | 0.0554 mL | 0.2770 mL | 0.5541 mL | 1.3851 mL | |
| 40 mM | 0.0416 mL | 0.2078 mL | 0.4155 mL | 1.0389 mL | |
| 50 mM | 0.0332 mL | 0.1662 mL | 0.3324 mL | 0.8311 mL | |
| 60 mM | 0.0277 mL | 0.1385 mL | 0.2770 mL | 0.6926 mL | |
| 80 mM | 0.0208 mL | 0.1039 mL | 0.2078 mL | 0.5194 mL |
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