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  3. Wheat germ agglutinin-AF488

Wheat germ agglutinin-AF488 (WGA-AF488) is a cell membrane-specific staining agent prepared by conjugating wheat germ agglutinin with the Alexa Fluor 488 (HY-D1304) fluorescent dye, and it binds to cell surface glycoproteins with high affinity. Wheat germ agglutinin-AF488 is applied in fluorescence microscopy and confocal imaging techniques, and it can clearly label the membrane structures of various cells including breast cancer cells, enabling high-resolution visual observation. Wheat germ agglutinin-AF488 is used in studies of breast cancer and triple-negative breast cancer to observe cell morphology and membrane dynamic changes.

For research use only. We do not sell to patients.

Wheat germ agglutinin-AF488

Wheat germ agglutinin-AF488 Chemical Structure

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Description

Wheat germ agglutinin-AF488 (WGA-AF488) is a cell membrane-specific staining agent prepared by conjugating wheat germ agglutinin with the Alexa Fluor 488 (HY-D1304) fluorescent dye, and it binds to cell surface glycoproteins with high affinity. Wheat germ agglutinin-AF488 is applied in fluorescence microscopy and confocal imaging techniques, and it can clearly label the membrane structures of various cells including breast cancer cells, enabling high-resolution visual observation. Wheat germ agglutinin-AF488 is used in studies of breast cancer and triple-negative breast cancer to observe cell morphology and membrane dynamic changes[1][2][3].

In Vitro

Wheat germ agglutinin-AF488 (1 μg/mL; 5 min) effectively stains the surface of HEK293 cells, allowing clear visualization of cell boundaries in fluorescence microscopy[1].
Wheat germ agglutinin-AF488 (5 μg/mL; 5 min at 37 °C) labels the membranes of human breast cancer cell lines HCC-1954, MDA-MB-231, and SKBR3 for visualization via confocal fluorescence microscopy[2].
Wheat germ agglutinin-AF488 (5 μg/mL; 5 min at 37 °C) labels the membranes of human breast cancer cell line HCC-1954 for visualization and cell boundary identification via confocal fluorescence microscopy[2].
Wheat germ agglutinin-AF488 (5 μg/mL) stains the cell membrane of MDA-MB-468 cells, enabling membrane visualization in confocal fluorescence imaging[3].
Wheat germ agglutinin-AF488 (5 μg/mL) stains the cell membrane of MDA-MB-468 cells, enabling visualization of membrane structure during time-dependent cellular uptake studies of targeted DNA origami[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Color

Yellow to orange

SMILES

[Wheat germ agglutinin-AF488]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Wheat germ agglutinin-AF488
Cat. No.:
HY-NP163A
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