Wheat germ agglutinin-AF555
Based on 1 publication(s) in Google Scholar
Wheat germ agglutinin-AF555 (WGA-AF555) is a membrane-staining lectin conjugate that combines wheat germ agglutinin with the Alexa Fluor 555 fluorescent dye. Wheat germ agglutinin-AF555 is used for precise staining and contour delineation of cell membranes. Wheat germ agglutinin-AF555 also effectively distinguishes between surface vimentin and intracellular vimentin in cells.
For research use only. We do not sell to patients.
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications Citing Use of MedChemExpress (MCE) Wheat germ agglutinin-AF555
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Biological Activity
Wheat germ agglutinin-AF555 (1:500 dilution; 1 hour) labels the surface of Caco-2 cells, enabling visualization of cell membrane structure via confocal microscopy[1].
Wheat germ agglutinin-AF555 (500 ng/mL; 30 min) labels the plasma membrane of fixed mammalian cells at a concentration of 500 ng/mL following a 30-minute room temperature incubation in the dark[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Caco-2 cells
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Concentration:1:500 dilution
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Incubation Time:1 hour (room temperature)
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Result:Stained the surface of Caco-2 cells, appearing as red fluorescence that outlined the cell membranes, allowing visualization of cell surface morphology alongside nuclear staining (DAPI, blue) and vimentin staining (Alexafluor-647, green).
Chemical Information
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Appearance Solid
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Color Purple to purplish red
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SMILES
[Wheat germ agglutinin-AF555]
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Synonyms
WGA-AF555
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (1)
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Journal Impact Factor
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Most Recent
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Cell
2026 Jul 9;189(14):4396-4414.e32. PMID: 42143020
Purity & Documentation
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Data Sheet (267 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)