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  4. Wheat germ agglutinin-AF555

Wheat germ agglutinin-AF555  (Synonyms: WGA-AF555)

Cat. No.: HY-NP163C Purity: 0.00%
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Handling Instructions Technical Support

Wheat germ agglutinin-AF555 (WGA-AF555) is a membrane-staining lectin conjugate that combines wheat germ agglutinin with the Alexa Fluor 555 fluorescent dye. Wheat germ agglutinin-AF555 is used for precise staining and contour delineation of cell membranes. Wheat germ agglutinin-AF555 also effectively distinguishes between surface vimentin and intracellular vimentin in cells.

For research use only. We do not sell to patients.

Wheat germ agglutinin-AF555

Wheat germ agglutinin-AF555 Chemical Structure

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Based on 1 publication(s) in Google Scholar

Other Forms of Wheat germ agglutinin-AF555:

Wheat germ agglutinin-AF555 Related Antibodies

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Description

Wheat germ agglutinin-AF555 (WGA-AF555) is a membrane-staining lectin conjugate that combines wheat germ agglutinin with the Alexa Fluor 555 fluorescent dye. Wheat germ agglutinin-AF555 is used for precise staining and contour delineation of cell membranes. Wheat germ agglutinin-AF555 also effectively distinguishes between surface vimentin and intracellular vimentin in cells[1][2].

In Vitro

Wheat germ agglutinin-AF555 (1:500 dilution; 1 hour) labels the surface of Caco-2 cells, enabling visualization of cell membrane structure via confocal microscopy[1].
Wheat germ agglutinin-AF555 (500 ng/mL; 30 min) labels the plasma membrane of fixed mammalian cells at a concentration of 500 ng/mL following a 30-minute room temperature incubation in the dark[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Wheat germ agglutinin-AF555 Related Antibodies

Immunofluorescence[1]

Cell Line: Caco-2 cells
Concentration: 1:500 dilution
Incubation Time: 1 hour (room temperature)
Result: Stained the surface of Caco-2 cells, appearing as red fluorescence that outlined the cell membranes, allowing visualization of cell surface morphology alongside nuclear staining (DAPI, blue) and vimentin staining (Alexafluor-647, green).
Appearance

Solid

Color

Purple to purplish red

SMILES

[Wheat germ agglutinin-AF555]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation

SDS (251 KB)

COA (230 KB)

Handling Instructions (2659 KB)
References
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Wheat germ agglutinin-AF555 Related Classifications

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Wheat germ agglutinin-AF555WGA-AF555Fluorescent Dyemammalian cellsconfocal microscopyCaco-2 cellsvimentinplasma membraneInhibitorinhibitorinhibit

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