2. GPCR/G Protein Neuronal Signaling NF-κB Autophagy
  3. Cannabinoid Receptor NF-κB Autophagy
  4. Yangonin

Yangonin 

Cat. No.: HY-N0919 Purity: 99.79%
COA Handling Instructions

Yangonin exhibits affinity for the human recombinant cannabinoid CB1 receptor with an IC50 and a Ki of 1.79 μM and 0.72 μM, respectively.

For research use only. We do not sell to patients.

Yangonin Chemical Structure

Yangonin Chemical Structure

CAS No. : 500-62-9

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 227 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 227 In-stock
Solid
1 mg USD 80 In-stock
5 mg USD 168 In-stock
10 mg USD 264 In-stock
50 mg   Get quote  
100 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Yangonin Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Yangonin

View All Cannabinoid Receptor Isoform Specific Products:

View All Isoforms
CB1 CB2

View All NF-κB Isoform Specific Products:

View All Isoforms
NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Yangonin exhibits affinity for the human recombinant cannabinoid CB1 receptor with an IC50 and a Ki of 1.79 μM and 0.72 μM, respectively.

IC50 & Target[1][2]

CB1

1.79 μM (IC50)

CB1

0.72 μM (Ki)

RelA

 

In Vitro

Yangonin is one of the six major kavalactones found in Piper methysticum.Yangonin potently inhibits NF-κB activation through suppression of the transcriptional activity of the RelA/p65 subunit of NF-κB. Yangonin significantly inhibits the induced expression of the NF-κB-reporter gene. However, Yangonin does not interfere with TNF-α-induced inhibitor of κBα (IκBα) degradation, p65 nuclear translocation, and DNA-binding activity of NF-κB. Yangonin inhibits not only the induced NF-κB activation by overexpression of RelA/p65, but also transactivation activity of RelA/p65. Yangonin does not inhibit TNF-α-induced activation of p38, but it significantly impairs activation of ERK 1/2 and stress-activated protein kinase/JNK[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Yangonin Related Antibodies
Molecular Weight

258.27

Appearance

Solid

Formula

C15H14O4
CAS No.
SMILES

O=C1C=C(OC)C=C(/C=C/C2=CC=C(OC)C=C2)O1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (96.80 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8719 mL 19.3596 mL 38.7192 mL
5 mM 0.7744 mL 3.8719 mL 7.7438 mL
10 mM 0.3872 mL 1.9360 mL 3.8719 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.5 mg/mL (9.68 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.79%

COA (245 KB) HNMR (266 KB) LCMS (265 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[2]

HeLa cells are seeded at 1×105 cells/mL in 96-well plates containing 100 μL of DMEM medium with 10% FBS and incubated overnight. Yangonin is dissolved in DMSO and DMSO is added to all plates to compensate the same volume of DMSO. After 24 h, the cells are pretreated with different concentrations of Yangonin (0.1-3 μM) for 1 h, followed by stimulation with or without TNF-α for 24 h. Subsequently, cells are cultured with MTT solution (5 mg/mL) for 3 h. The viable cells convert MTT to formazan, which generates a blue-purple color after dissolving in 150 μL of DMSO. The absorbance at 570 nm is measured by an ELISA plate reader[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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Yangonin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

Yangonin500-62-9Cannabinoid ReceptorNF-κBAutophagyNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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