1. Cell Cycle/DNA Damage
    Antibody-drug Conjugate/ADC Related
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  2. Topoisomerase
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  3. Camptothecin

Camptothecin  (Synonyms: Campathecin; (S)-(+)-Camptothecin; CPT)

Cat. No.: HY-16560 Purity: 99.69%
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Camptothecin (CPT), a kind of alkaloid, is a DNA topoisomerase I (Topo I) inhibitor with an IC50 of 679 nM. Camptothecin (CPT) exhibits powerful antineoplastic activity against colorectal, breast, lung and ovarian cancers, modulates hypoxia-inducible factor-1α (HIF-1α) activity by changing microRNAs (miRNA) expression patterns in human cancer cells.

For research use only. We do not sell to patients.

Camptothecin Chemical Structure

Camptothecin Chemical Structure

CAS No. : 7689-03-4

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Customer Review

Based on 32 publication(s) in Google Scholar

Top Publications Citing Use of Products

29 Publications Citing Use of MCE Camptothecin

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    Camptothecin purchased from MCE. Usage Cited in: Nat Commun. 2018 Oct 8;9(1):4139.  [Abstract]

    One hour after treated with UV or 2 h after treated with MMS, Camptothecin (CPT) (10 μM), or Doxorubicin (DOX; 0.5 μM), HEK293T cells expressing HA-SUMO2 and His-RhoB are subjected to sumoylation assay to detect the SUMO2 conjugation of RhoB.

    Camptothecin purchased from MCE. Usage Cited in: Nat Commun. 2019 Aug 21;10(1):3761.  [Abstract]

    Genotoxic stress-induced β-catenin signaling is activated via a TCF- or FOXO-independent mechanism. Representative images of the subcellular localization of β-catenin in the indicated cells treated with CPT (10 μM, 1 h), IR (10 Gy), and CDDP (10 μM, 1 h), as analyzed by immunofluorescence staining.

    Camptothecin purchased from MCE. Usage Cited in: Cell Death Differ. 2020 Nov;27(11):3162-3176.  [Abstract]

    293T cells transfected with Flag-TopBP1 and GFP-tagged RBMX and its domain (RRM, RBM1CTR, and C-RBD) are treated with 100 nM CPT for 24 h and subjected to immunoprecipitation with Flag-beads. Immunoprecipitates are immunoblotted with Flag and GFP antibodies, respectively.
    • Biological Activity

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    Description

    Camptothecin (CPT), a kind of alkaloid, is a DNA topoisomerase I (Topo I) inhibitor with an IC50 of 679 nM[1]. Camptothecin (CPT) exhibits powerful antineoplastic activity against colorectal, breast, lung and ovarian cancers, modulates hypoxia-inducible factor-1α (HIF-1α) activity by changing microRNAs (miRNA) expression patterns in human cancer cells[2][3].

    IC50 & Target[3]

    Topoisomerase I

    679 nM (IC50)

    Camptothecins

     

    In Vitro

    High TOP1 enzymatic activity MCF7 (Luminal subtype) and HCC1419 (HER2 subtype) show high sensitivity toward Camptothecin (0.1 μM to 5 μM; 72 hours) treatment, exhibiting the IC50 values of 0.089 μM and 0.067 μM, respectively[4].
    Camptothecin (0.5 μM; 6 and 24 hours) reduces desferrioxamine-activated VEGF expression.
    Camptothecin (0.5 μM; 8 to 24 hours) strongly prevents the desferrioxamine-dependent HIF-1a accumulation[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[4]

    Cell Line: MCF7 (Luminal subtype) and HCC1419 (HER2 subtype)
    Concentration: 0.1 μM to 5 μM
    Incubation Time: 72 hours
    Result: High TOP1 enzymatic activity MCF7 (Luminal subtype) and HCC1419 (HER2 subtype) show high sensitivity toward CPT (0.1 μM to 5 μM; 72 hours) treatment, exhibiting the IC50 values of 0.089 μM and 0.067 μM, respectively.

    RT-PCR[2]

    Cell Line: HeLa and HEK293 cell lines
    Concentration: 0.5 μmol/L
    Incubation Time: 6 and 24 hours
    Result: Reduces desferrioxamine-activated VEGF expression in both cell lines after 6 and 24 hours of treatment, whereas in normoxic condition camptothecin does not affect the VEGF mRNA level.

    Western Blot Analysis[2]

    Cell Line: HeLa and HEK293 cell lines
    Concentration: 0.5 μmol/L
    Incubation Time: 8 to 24 hours
    Result: Strongly prevents the desferrioxamine-dependent HIF-1a accumulation after 8 to 24 hours, whereas it does not affect HIF-1b levels.
    In Vivo

    Camptothecin (2 mg/kg every other day) treats mice, has developed numerous pulmonary metastases.
    Treatment with both kinase inhibitor of nuclear factor-kappaB-1 (KINK-1) and Camptothecin led to a statistically significant reduction in the number of pulmonary metastases[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C57BL6 mice (injected with B16F10 melanoma cells)[5]
    Dosage: 2 mg/kg
    Administration: every other day, after 19 days
    Result: Has developed numerous pulmonary metastases.
    Clinical Trial
    Molecular Weight

    348.35

    Formula

    C20H16N2O4

    CAS No.
    SMILES

    O=C1C2=C([[email protected]@](O)(CC)C(OC2)=O)C=C(N1C3)C(C3=C4)=NC5=C4C=CC=C5

    Structure Classification
    Source
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 6.25 mg/mL (17.94 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8707 mL 14.3534 mL 28.7068 mL
    5 mM 0.5741 mL 2.8707 mL 5.7414 mL
    10 mM 0.2871 mL 1.4353 mL 2.8707 mL
    *Please refer to the solubility information to select the appropriate solvent.
    Purity & Documentation

    Purity: 99.69%

    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
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    Cat. No.:
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