CL097 hydrochloride
Based on 3 publication(s) in Google Scholar
CL097, a potent TLR7 and TLR8 agonist, induces pro-inflammatory cytokines in macrophages. CL097 induces NADPH oxidase priming, resulting in an increase of the fMLF-stimulated ROS production.
For research use only. We do not sell to patients.
- Purity: 99.96%
- CAS No.: 2990770-48-2
- Formula: C13H15ClN4O
- Molecular Weight:278.74
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) CL097 hydrochloride
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Biological Activity
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TLR7 |
TLR8 |
CL097 induces activation of NF-κB at 0.1 μM in TLR7 transfected HEK293 cells and at 4 μM in TLR8-transfected HEK293 cells[1].
CL097 induces hyperactivation of the NADPH oxidase by stimulating the phosphorylation of p47phox on selective sites in human neutrophils and suggest that p38 MAPK, ERK1/2, protein kinase C, and Pin1 control this process. CL097 induces the phosphorylation of p47phox on specific sites and enhances fMLF-induced p47phox phosphorylation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Neutrophils
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Concentration:0, 0.5, 2.5, 5, and 10 μg/mL
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Incubation Time:Pretreated for 30 minutes
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Result:Induced phosphorylation of p47phox on specific sites in a concentration-dependent manner.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female 8.3 NOD mice (5-6 weeks old)[3]
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Dosage:5 mg/kg
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Administration:Injected s.c.
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Result:Caused a modest specific lysis of the target peptide (∼25%).
Chemical Information
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CAS No. 2990770-48-2
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Appearance Solid
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Molecular Weight 278.74
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Formula C13H15ClN4O
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Color White to off-white
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SMILES
NC1=NC2=CC=CC=C2C3=C1NC(COCC)=N3.[H]Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (3)
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Journal Impact Factor
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Most Recent
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Cell Res
2025 Apr 1. PMID: 40164768 -
Cell Rep
Restraint of inflammasome-driven cytokine responses through the mRNA stability protein TTP. [Abstract]2025 Feb 20;44(3):115340. PMID: 39982821
CL097 hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Feb 20;44(3):115340. [Abstract]
iBMDMs were primed with LPS and stimulated with 70 μM CL097 over a time course of 45 min. Cellular lysates were examined by western blot for changes in ERK1/2 phosphorylation and GSDMD cleavage.
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Int Immunopharmacol
Repurposing AZD-7762 as a novel direct NLRP3 inhibitor for the treatment of inflammatory diseases. [Abstract]2026 Jun 25:186:117053. PMID: 42348984
Solvent & Solubility
DMSO : 100 mg/mL (358.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 5 mg/mL (17.94 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.97 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.97 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Cindy Patinote, et al. Agonist and antagonist ligands of toll-like receptors 7 and 8: Ingenious tools for therapeutic purposes. Eur J Med Chem. 2020 May 1;193:112238. [Content Brief]
[2]. Karama Makni-Maalej, et al. The TLR7/8 agonist CL097 primes N-formyl-methionyl-leucyl-phenylalanine-stimulated NADPH oxidase activation in human neutrophils: critical role of p47phox phosphorylation and the proline isomerase Pin1. J Immunol. 2012 Nov 1;189(9):4657-65. [Content Brief]
[3]. A S Lee, et al. Toll-like receptor 7 stimulation promotes autoimmune diabetes in the NOD mouse. Diabetologia. 2011 Jun;54(6):1407-16. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.5876 mL | 17.9379 mL | 35.8757 mL | 89.6893 mL |
| 5 mM | 0.7175 mL | 3.5876 mL | 7.1751 mL | 17.9379 mL | |
| 10 mM | 0.3588 mL | 1.7938 mL | 3.5876 mL | 8.9689 mL | |
| 15 mM | 0.2392 mL | 1.1959 mL | 2.3917 mL | 5.9793 mL | |
| DMSO | 20 mM | 0.1794 mL | 0.8969 mL | 1.7938 mL | 4.4845 mL |
| 25 mM | 0.1435 mL | 0.7175 mL | 1.4350 mL | 3.5876 mL | |
| 30 mM | 0.1196 mL | 0.5979 mL | 1.1959 mL | 2.9896 mL | |
| 40 mM | 0.0897 mL | 0.4484 mL | 0.8969 mL | 2.2422 mL | |
| 50 mM | 0.0718 mL | 0.3588 mL | 0.7175 mL | 1.7938 mL | |
| 60 mM | 0.0598 mL | 0.2990 mL | 0.5979 mL | 1.4948 mL | |
| 80 mM | 0.0448 mL | 0.2242 mL | 0.4484 mL | 1.1211 mL | |
| 100 mM | 0.0359 mL | 0.1794 mL | 0.3588 mL | 0.8969 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
CL097 hydrochloride Related Classifications
HY-128799A Related Classifications
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