1. Immunology/Inflammation
    NF-κB
    Metabolic Enzyme/Protease
  2. Toll-like Receptor (TLR)
    Reactive Oxygen Species
  3. CL097

CL097 

Cat. No.: HY-128799 Purity: 99.89%
COA Handling Instructions

CL097, a potent TLR7 and TLR8 agonist, induces pro-inflammatory cytokines in macrophages. CL097 induces NADPH oxidase priming, resulting in an increase of the fMLF-stimulated ROS production.

For research use only. We do not sell to patients.

CL097 Chemical Structure

CL097 Chemical Structure

CAS No. : 1026249-18-2

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5 mg USD 300 In-stock
Estimated Time of Arrival: December 31
10 mg USD 450 In-stock
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25 mg USD 950 In-stock
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50 mg USD 1500 In-stock
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100 mg USD 2400 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

CL097, a potent TLR7 and TLR8 agonist, induces pro-inflammatory cytokines in macrophages[1]. CL097 induces NADPH oxidase priming, resulting in an increase of the fMLF-stimulated ROS production[2].

IC50 & Target

TLR7

 

TLR8

 

In Vitro

CL097 induces activation of NF-κB at 0.1 μM in TLR7 transfected HEK293 cells and at 4 μM in TLR8-transfected HEK293 cells[1].
CL097 induces hyperactivation of the NADPH oxidase by stimulating the phosphorylation of p47phox on selective sites in human neutrophils and suggest that p38 MAPK, ERK1/2, protein kinase C, and Pin1 control this process. CL097 induces the phosphorylation of p47phox on specific sites and enhances fMLF-induced p47phox phosphorylation[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[2]

Cell Line: Neutrophils
Concentration: 0, 0.5, 2.5, 5, and 10 μg/mL
Incubation Time: Pretreated for 30 minutes
Result: Induced phosphorylation of p47phox on specific sites in a concentration-dependent manner.
In Vivo

CL097 and CD40 agonist stimulation induces efficient diabetogenic Cytotoxic T lymphocyte (CTL) function in NOD mice. CL097 (5 mg/kg, s.c.) alone causes a modest specific lysis of the target peptide (∼25%). However, treatment with a combination of CL097 and CD40 agonist (10 mg/kg, i.p.) results in an increase of approximately twofold in the specific lysis of the IGRP-peptide-coated targets compared with CL097 treatment alone[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female 8.3 NOD mice (5-6 weeks old)[3]
Dosage: 5 mg/kg
Administration: Injected s.c.
Result: Caused a modest specific lysis of the target peptide (∼25%).
Molecular Weight

242.28

Formula

C13H14N4O

CAS No.
SMILES

NC1=NC2=CC=CC=C2C3=C1NC(COCC)=N3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (412.75 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1275 mL 20.6373 mL 41.2746 mL
5 mM 0.8255 mL 4.1275 mL 8.2549 mL
10 mM 0.4127 mL 2.0637 mL 4.1275 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (10.32 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (10.32 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (10.32 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.89%

References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
CL097
Cat. No.:
HY-128799
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