2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Toll-like Receptor (TLR) Reactive Oxygen Species (ROS)
  4. CL097

CL097, a potent TLR7 and TLR8 agonist, induces pro-inflammatory cytokines in macrophages. CL097 induces NADPH oxidase priming, resulting in an increase of the fMLF-stimulated ROS production.

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CAS No. : 1026249-18-2

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of CL097:

CL097 Related Antibodies

Top Publications Citing Use of Products

    CL097 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Feb 20;44(3):115340.  [Abstract]

    iBMDMs were primed with LPS and stimulated with 70 μM CL097 over a time course of 45 min. Cellular lysates were examined by western blot for changes in ERK1/2 phosphorylation and GSDMD cleavage.

    View All Toll-like Receptor (TLR) Isoform Specific Products:

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    Description

    CL097, a potent TLR7 and TLR8 agonist, induces pro-inflammatory cytokines in macrophages[1]. CL097 induces NADPH oxidase priming, resulting in an increase of the fMLF-stimulated ROS production[2].

    IC50 & Target

    TLR7

     

    TLR8

     

    In Vitro

    CL097 induces activation of NF-κB at 0.1 μM in TLR7 transfected HEK293 cells and at 4 μM in TLR8-transfected HEK293 cells[1].
    ? CL097 induces hyperactivation of the NADPH oxidase by stimulating the phosphorylation of p47phox on selective sites in human neutrophils and suggest that p38 MAPK, ERK1/2, protein kinase C, and Pin1 control this process. CL097 induces the phosphorylation of p47phox on specific sites and enhances fMLF-induced p47phox phosphorylation[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    CL097 Related Antibodies

    Western Blot Analysis[2]

    Cell Line: Neutrophils
    Concentration: 0, 0.5, 2.5, 5, and 10 μg/mL
    Incubation Time: Pretreated for 30 minutes
    Result: Induced phosphorylation of p47phox on specific sites in a concentration-dependent manner.
    In Vivo

    CL097 and CD40 agonist stimulation induces efficient diabetogenic Cytotoxic T lymphocyte (CTL) function in NOD mice. CL097 (5 mg/kg, s.c.) alone causes a modest specific lysis of the target peptide (～25%). However, treatment with a combination of CL097 and CD40 agonist (10 mg/kg, i.p.) results in an increase of approximately twofold in the specific lysis of the IGRP-peptide-coated targets compared with CL097 treatment alone[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female 8.3 NOD mice (5-6 weeks old)[3]
    Dosage: 5 mg/kg
    Administration: Injected s.c.
    Result: Caused a modest specific lysis of the target peptide (∼25%).
    Molecular Weight

    242.28

    Formula

    C13H14N4O
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NC1=NC2=CC=CC=C2C3=C1NC(COCC)=N3
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (412.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.1275 mL 20.6373 mL 41.2746 mL
    5 mM 0.8255 mL 4.1275 mL 8.2549 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (10.32 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (10.32 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.90%

    SDS (251 KB)

    COA (251 KB) HNMR (271 KB) LCMS (93 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.1275 mL 20.6373 mL 41.2746 mL 103.1864 mL
    5 mM 0.8255 mL 4.1275 mL 8.2549 mL 20.6373 mL
    10 mM 0.4127 mL 2.0637 mL 4.1275 mL 10.3186 mL
    15 mM 0.2752 mL 1.3758 mL 2.7516 mL 6.8791 mL
    20 mM 0.2064 mL 1.0319 mL 2.0637 mL 5.1593 mL
    25 mM 0.1651 mL 0.8255 mL 1.6510 mL 4.1275 mL
    30 mM 0.1376 mL 0.6879 mL 1.3758 mL 3.4395 mL
    40 mM 0.1032 mL 0.5159 mL 1.0319 mL 2.5797 mL
    50 mM 0.0825 mL 0.4127 mL 0.8255 mL 2.0637 mL
    60 mM 0.0688 mL 0.3440 mL 0.6879 mL 1.7198 mL
    80 mM 0.0516 mL 0.2580 mL 0.5159 mL 1.2898 mL
    100 mM 0.0413 mL 0.2064 mL 0.4127 mL 1.0319 mL
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    CL097 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    CL0971026249-18-2CL 097CL-097Toll-like Receptor (TLR)Reactive Oxygen Species (ROS)TLR7TLR8ImmunologicalmodulatorsHeterocycleOligonucleotidePeptideInhibitorinhibitorinhibit

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