2. Membrane Transporter/Ion Channel Neuronal Signaling
  3. nAChR
  4. N-Methylcytisine

N-Methylcytisine  (Synonyms: Caulophylline)

Cat. No.: HY-N0443 Purity: 99.81%
COA
SDS
Handling Instructions Technical Support

N-Methylcytisine (Caulophylline), a tricyclic quinolizidine alkaloid, exerts hypoglycaemic, analgesic and anti-inflammatory activities. N-methylcytisine is a selective ligand of nicotinic receptors of acetylcholine in the central nervous system and has a high affinity (Kd = 50 nM) to nicotinic acetylcholine receptors (nAChR) from squid optical ganglia.

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N-Methylcytisine

N-Methylcytisine 화학구조

CAS No. : 486-86-2

사이즈 가격 재고 수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 해외재고보유
Solution
10 mM * 1 mL in DMSO 해외재고보유
Solid
5 mg 해외재고보유
10 mg 해외재고보유
25 mg 해외재고보유
50 mg 해외재고보유
100 mg   견적 받기  
200 mg   견적 받기  

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 2 publication(s) in Google Scholar

Other Forms of N-Methylcytisine:

N-Methylcytisine Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • 순도&문서

  • References

  • 고객리뷰

제품 설명

N-Methylcytisine (Caulophylline), a tricyclic quinolizidine alkaloid, exerts hypoglycaemic, analgesic and anti-inflammatory activities. N-methylcytisine is a selective ligand of nicotinic receptors of acetylcholine in the central nervous system and has a high affinity (Kd = 50 nM) to nicotinic acetylcholine receptors (nAChR) from squid optical ganglia[1][2].

IC50 & Target

kd: 50 nM (nicotinic acetylcholine receptors)[2]
In Vivo

N-Methylcytisine (Compound NMC) (4-16 mg/kg, i.g., once per day) ameliorates the symptoms of DSS-induced colitis in mouse model[1].
N-Methylcytisine (16 mg/kg, p.o., once per day) significantly reduces the concentrations of TNF-α, IL-1β, and IL-6 as well as inhibiting the expression of p-NK-κB p65 in the colon tissue of mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: DSS-induced colitis ICR mouse model (18-22 g)[1]
Dosage: 4 and 16 mg/kg
Administration: Intragastrically (i.g.) once a day
Result: Markedly reduced the disease activity index score.
Evidently improved the symptom of DSS-induced colon shortening.
Ameliorated severe inflammation-induced damage exhibited in the DSS-induced colitis group.
Decreased the activity of myeloperoxidase in the colon.
분자량

204.27

화학식

C12H16N2O
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1C=CC=C2N1C[C@]3([H])CN(C)C[C@@]2([H])C3
Structure Classification
Initial Source
선적

Room temperature in continental US; may vary elsewhere.
보관

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

용액&용해도
In Vitro: 

DMSO : 120 mg/mL (587.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 100 mg/mL (489.54 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.8954 mL 24.4770 mL 48.9541 mL
5 mM 0.9791 mL 4.8954 mL 9.7908 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • 몰농도 계산기

  • 농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (10.18 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (10.18 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
순도&문서

Purity: 99.81%

SDS (393 KB)

COA (253 KB) RP-HPLC (244 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 4.8954 mL 24.4770 mL 48.9541 mL 122.3852 mL
5 mM 0.9791 mL 4.8954 mL 9.7908 mL 24.4770 mL
10 mM 0.4895 mL 2.4477 mL 4.8954 mL 12.2385 mL
15 mM 0.3264 mL 1.6318 mL 3.2636 mL 8.1590 mL
20 mM 0.2448 mL 1.2239 mL 2.4477 mL 6.1193 mL
25 mM 0.1958 mL 0.9791 mL 1.9582 mL 4.8954 mL
30 mM 0.1632 mL 0.8159 mL 1.6318 mL 4.0795 mL
40 mM 0.1224 mL 0.6119 mL 1.2239 mL 3.0596 mL
50 mM 0.0979 mL 0.4895 mL 0.9791 mL 2.4477 mL
60 mM 0.0816 mL 0.4080 mL 0.8159 mL 2.0398 mL
80 mM 0.0612 mL 0.3060 mL 0.6119 mL 1.5298 mL
100 mM 0.0490 mL 0.2448 mL 0.4895 mL 1.2239 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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N-Methylcytisine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-Methylcytisine486-86-2CaulophyllinenAChRNicotinic acetylcholine receptorsInhibitorinhibitorinhibit

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상품명:
N-Methylcytisine
Cat. No.:
HY-N0443
수량:
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