2. GPCR/G Protein Neuronal Signaling
  3. mAChR
  4. Otenzepad

Otenzepad  (Synonyms: AF-DX 116)

Cat. No.: HY-101381 Purity: 99.92%
COA Handling Instructions

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Otenzepad (AF-DX 116) is a selective and competitive M2 muscarinic acetylcholine receptor antagonist, with IC50 values of 640 nM and 386 nM for rabbit peripheral lung and rat heart, respectively.

For research use only. We do not sell to patients.

Otenzepad Chemical Structure

Otenzepad Chemical Structure

CAS No. : 102394-31-0

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 105 In-stock
Solution
10 mM * 1 mL in DMSO USD 105 In-stock
Solid
1 mg USD 50 In-stock
5 mg USD 95 In-stock
10 mg USD 165 In-stock
25 mg USD 345 In-stock
50 mg USD 550 In-stock
100 mg USD 950 In-stock
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Based on 1 publication(s) in Google Scholar

Otenzepad Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Otenzepad

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Otenzepad (AF-DX 116) is a selective and competitive M2 muscarinic acetylcholine receptor antagonist, with IC50 values of 640 nM and 386 nM for rabbit peripheral lung and rat heart, respectively[1].

IC50 & Target

640 nM (M2 muscarinic acetylcholine receptor in rabbit peripheral lung), 386 nM (M2 muscarinic acetylcholine receptor in rat heart)[1].
In Vivo

Otenzepad (0.5, 1 mg/kg, s.c., in rats) significantly improved win-stay acquisition relative to vehicle-injected controls[2].
Otenzepad (2 mg/kg, s.c., in rats) significantly improved retention relative to vehicle controls[2].
Otenzepad (0.3, 1.0, or 3.0 mg/kg, ip, in mice) potentiates the effects of glucose and reverses the effects of insulin on memory[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Forty-eight male Long-Evans rats (325-350 g)[2].
Dosage: 0.25, 0.5, 1.0 and 2.0 mg/kg.
Administration: S.C. on the dorsum of the neck once.
Result: Doses of 0.5 and 1.0 mg/kg significantly improved acquisition relative to vehicle controls, while doses of 0.25 and 2.0 mg/kg had no effect.
Animal Model: Adult male Swiss mice (age 60–70 days; weight 25-30 g)[3].
Dosage: 0.3, 1.0, or 3.0 mg/kg.
Administration: IP once.
Result: Enhanced retention in an inverted-U dose–response manner, with significant enhancement seen at 1.0 mg/kg (U15,15 = 49, p < 0.02, compared with saline-saline-injected control group).
Molecular Weight

421.54

Formula

C24H31N5O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1NC2=CC=CN=C2N(C(CN3C(CN(CC)CC)CCCC3)=O)C4=CC=CC=C14
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (59.31 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3723 mL 11.8613 mL 23.7225 mL
5 mM 0.4745 mL 2.3723 mL 4.7445 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.92%

COA (258 KB) HNMR (269 KB) LCMS (269 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3723 mL 11.8613 mL 23.7225 mL 59.3064 mL
5 mM 0.4745 mL 2.3723 mL 4.7445 mL 11.8613 mL
10 mM 0.2372 mL 1.1861 mL 2.3723 mL 5.9306 mL
15 mM 0.1582 mL 0.7908 mL 1.5815 mL 3.9538 mL
20 mM 0.1186 mL 0.5931 mL 1.1861 mL 2.9653 mL
25 mM 0.0949 mL 0.4745 mL 0.9489 mL 2.3723 mL
30 mM 0.0791 mL 0.3954 mL 0.7908 mL 1.9769 mL
40 mM 0.0593 mL 0.2965 mL 0.5931 mL 1.4827 mL
50 mM 0.0474 mL 0.2372 mL 0.4745 mL 1.1861 mL
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Otenzepad Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Otenzepad102394-31-0AF-DX 116mAChRMuscarinic acetylcholine receptormemory,Inhibitorinhibitorinhibit

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