Oxelumab  (Synonyms: R 4930; huMAb OX 40L; Ro 49-89991)

Oxelumab (R 4930) is a human monoclonal antibody against the OX40 ligand (OX40L). Oxelumab can be used for the research of asthma^[1].

Human IgG1 kappa

Human IgG1 kappa, Isotype Control

Human

TNFSF4/OX40L/CD252

Oxelumab (10 μg/mL; 3 d) represses cell proliferation of NK cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Oxelumab (5 and 30 mg/kg; i.p., once daily for twice) affects the concentrations of IL-6 and the frequencies of splenic human effector memory T cells and switched B cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

7292  [NCBI]

P23510

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

145.57 kDa

1186098-83-8

Liquid

Colorless to light yellow

[Oxelumab]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Human IgG1 kappa

• 
  Immobilized OX40 Ligand/TNFSF4 Protein, Human (HEK293, HY-P700306) can bind Oxelumab. The EC₅₀ for this effect is 60.57 ng/mL.
• 
  Flow cytometric analysis of 1X10⁶ HUVEC cells with Oxelumab (HY-P99408, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

• [1]. Youngblood BA, et al. AK002, a Humanized Sialic Acid-Binding Immunoglobulin-Like Lectin-8 Antibody that Induces Antibody-Dependent Cell-Mediated Cytotoxicity against Human Eosinophils and Inhibits Mast Cell-Mediated Anaphylaxis in Mice. Int Arch Allergy Immunol. 2019;180(2):91-102.  [Content Brief]

  [2]. Kweon S, et al. Expansion of Human NK Cells Using K562 Cells Expressing OX40 Ligand and Short Exposure to IL-21. Front Immunol. 2019 Apr 24;10:879.  [Content Brief]