2. Anti-infection NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
  3. Bacterial Influenza Virus Reactive Oxygen Species (ROS) Endogenous Metabolite
  4. rel-α-Vitamin E

rel-α-Vitamin E  (Synonyms: rel-D-α-Tocopherol)

製品番号: HY-N0683A 純度: 70.0%
COA 取扱説明書 Technical Support

rel-α-Vitamin E (rel-D-α-Tocopherol) is a vitamin with antioxidant properties and also a mixture.

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rel-α-Vitamin E

rel-α-Vitamin E 構造式

CAS 番号 : 2074-53-5

容量 価格（税別） 在庫状況 数量
Oil + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 41 在庫あり
Solution
10 mM * 1 mL in DMSO USD 41 在庫あり
Oil
5 mg $38 在庫あり
10 mg $60 在庫あり
50 mg   お問い合わせ  
100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Other Forms of rel-α-Vitamin E:

rel-α-Vitamin E 関連抗体

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Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
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製品説明

rel-α-Vitamin E (rel-D-α-Tocopherol) is a vitamin with antioxidant properties and also a mixture[1].

Cellular Effect
Cell Line Type Value Description References
Erythrocyte IC50
3.658 μg/mL
Compound: alpha-tocopherol
Antioxidant activity in rat erythrocytes assessed as inhibition of phenyl hydrazine-induced haemolysis after 1 hr
Antioxidant activity in rat erythrocytes assessed as inhibition of phenyl hydrazine-induced haemolysis after 1 hr
[PMID: 23395966]
Fibroblast EC50
>500 nM
Compound: alpha-TOH
Inhibition of erastin-induced cell death in FRDA patient-derived fibroblast assessed as depletion of cellular ATP incubated for 12 hrs followed by erastin stimulation and measured after by luciferase-linked ATPase enzymatic assay
Inhibition of erastin-induced cell death in FRDA patient-derived fibroblast assessed as depletion of cellular ATP incubated for 12 hrs followed by erastin stimulation and measured after by luciferase-linked ATPase enzymatic assay
[PMID: 33214825]
Fibroblast EC50
234 nM
Compound: 2, Vitamin E
Cytoprotective activity against BSO-induced human FRDA primary patient fibroblasts compound pretreated 12 hrs prior to BSO-induction measured after 48 hrs by calcein-AM-based cell viability assay
Cytoprotective activity against BSO-induced human FRDA primary patient fibroblasts compound pretreated 12 hrs prior to BSO-induction measured after 48 hrs by calcein-AM-based cell viability assay
[PMID: 22137789]
Fibroblast GI50
>=300 μM
Compound: 2, Vitamin E
Growth inhibition of human FRDA primary patient fibroblasts after 48 hrs by calcein-AM-based cell viability assay
Growth inhibition of human FRDA primary patient fibroblasts after 48 hrs by calcein-AM-based cell viability assay
[PMID: 22137789]
HepG2 IC50
>300 μM
Compound: alpha-TOH
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 27344490]
HL-60 IC50
255 μM
Compound: Vitamin E
Antioxidant activity against TPA-induced ROS production in human HL60 cells by 2',7'-dichlorodihydrofluorescein diacetate cellular-based assay
Antioxidant activity against TPA-induced ROS production in human HL60 cells by 2',7'-dichlorodihydrofluorescein diacetate cellular-based assay
[PMID: 12762791]
J774 IC50
>500 μM
Compound: 1
Cytotoxicity against mouse J774 cells by MTT assay
Cytotoxicity against mouse J774 cells by MTT assay
[PMID: 17627828]
Lymphocyte EC50
>500 nM
Compound: alpha-TOH
Anti-ferroptotic activity in FRDA patient-derived Lymphocyte assessed as reduction in RSL3-induced lipid peroxidation incubated for overnight followed by RSL3 stimulation and measured after 90 mins by FACS analysis
Anti-ferroptotic activity in FRDA patient-derived Lymphocyte assessed as reduction in RSL3-induced lipid peroxidation incubated for overnight followed by RSL3 stimulation and measured after 90 mins by FACS analysis
[PMID: 33214825]
MCF7 IC50
>300 μM
Compound: alpha-TOH
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 27344490]
MCF7 IC50
329 μM
Compound: 1 (alpha-TOH)
Compound was tested for antiproliferative activity against human breast cancer cell line MCF-7 in MTT assay
Compound was tested for antiproliferative activity against human breast cancer cell line MCF-7 in MTT assay
[PMID: 9873556]
MT4 IC50
165 μM
Compound: alpha-TOH
Cytotoxicity against human MT4 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human MT4 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 27344490]
RPMI-8226 IC50
>300 μM
Compound: alpha-TOH
Cytotoxicity against human RPMI8226 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human RPMI8226 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 27344490]
THP-1 IC50
>500 μM
Compound: 1
Cytotoxicity against human THP1 cells by MTT assay
Cytotoxicity against human THP1 cells by MTT assay
[PMID: 17627828]
U-937 IC50
100 μM
Compound: alpha-TOH
Cytotoxicity against human U937 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human U937 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 27344490]
体外実験

Vitamin E is a lipid-soluble, chain-breaking type of antioxidant present in human blood. Vitamin E works as a free radical scavenger and has the primary function of destroying peroxyl radicals. Thus, it protects long-chain polyunsaturated fatty acids (e.g., cell membranes or low-density lipoprotein cholesterol) from oxidation or destruction. Its association with vitamin C is of great pathophysiological importance, because inhibition of lipid peroxidation by α-tocopherol occurs through its conversion into an oxidized α-tocopheroxyl radical, which in turn is regenerated to α-tocopherol through reduction by redox-active reagents[1].
α-Tocopherol can inhibit the recruitment of lymphocytes and eosinophils[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

rel-α-Vitamin E 関連抗体
体内実験

In order to longitudinally relate vitamin E status, evaluated by means of α- and γ-tocopherol measured in erythrocytes membranes, to inflammatory state and oxidative stress by means of total antioxidant capacity of plasma and lipid peroxidation biomarkers, and to assess its association to hard long-term outcomes (e.g., overall and cause-specific graft and recipient loss), and to be able to likewise account for liver, lung, and heart transplant recipients[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

430.72

分子式

C29H50O2
CAS 番号
Appearance

Oil

Color

Light yellow to yellow

SMILES

OC1=C(C)C(C)=C2C(CC[C@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)O2)=C1C
Structure Classification
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Pure form -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

Ethanol : 50 mg/mL (116.08 mM; Need ultrasonic)

DMSO : 16.11 mg/mL (37.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3217 mL 11.6085 mL 23.2169 mL
5 mM 0.4643 mL 2.3217 mL 4.6434 mL
10 mM 0.2322 mL 1.1608 mL 2.3217 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.61 mg/mL (3.74 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.61 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.1 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 1.61 mg/mL (3.74 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.61 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.1 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / Ethanol 1 mM 2.3217 mL 11.6085 mL 23.2169 mL 58.0423 mL
5 mM 0.4643 mL 2.3217 mL 4.6434 mL 11.6085 mL
10 mM 0.2322 mL 1.1608 mL 2.3217 mL 5.8042 mL
15 mM 0.1548 mL 0.7739 mL 1.5478 mL 3.8695 mL
20 mM 0.1161 mL 0.5804 mL 1.1608 mL 2.9021 mL
25 mM 0.0929 mL 0.4643 mL 0.9287 mL 2.3217 mL
30 mM 0.0774 mL 0.3869 mL 0.7739 mL 1.9347 mL
Ethanol 40 mM 0.0580 mL 0.2902 mL 0.5804 mL 1.4511 mL
50 mM 0.0464 mL 0.2322 mL 0.4643 mL 1.1608 mL
60 mM 0.0387 mL 0.1935 mL 0.3869 mL 0.9674 mL
80 mM 0.0290 mL 0.1451 mL 0.2902 mL 0.7255 mL
100 mM 0.0232 mL 0.1161 mL 0.2322 mL 0.5804 mL
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rel-α-Vitamin E Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

rel-α-Vitamin E2074-53-5rel-D-α-TocopherolBacterialInfluenza VirusReactive Oxygen Species (ROS)Endogenous MetaboliteMixtureantioxidantα-tocopheroloxidativestressinflammationbiomarkerInhibitorinhibitorinhibit

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