Search Result
Results for "
FRET
" in MedChemExpress (MCE) Product Catalog:
1
Biochemical Assay Reagents
2
Isotope-Labeled Compounds
Cat. No. |
Product Name |
Target |
Research Areas |
Chemical Structure |
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- HY-P2498A
-
|
Cathepsin
|
Cancer
|
Cathepsin D and E FRET Substrate acetate is a fluorogenic substrate for cathepsins D and E and not for B, H or L. The cleavage occurs at the Phe-Phe amide bond resul. Cathepsin D and E FRET Substrate is a valuable tool for routine assays and for mechanistic studies on cathepsins E and D .
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-
-
- HY-W027544
-
|
Fluorescent Dye
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Others
|
MCA is a coumarin derivative. MCA quantitates platelet-activating factor by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities .
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-
-
- HY-P2498
-
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Cathepsin
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Others
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Cathepsin D and E FRET Substrate is a fluorogenic substrate for cathepsins D and E and not for B, H or L. The cleavage occurs at the Phe-Phe amide bond resul. Cathepsin D and E FRET Substrate is a valuable tool for routine assays and for mechanistic studies on cathepsins E and D .
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-
-
- HY-161352
-
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Others
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Infection
|
NPI52 (Compound E1) is a foot-and-mouth disease virus 3C protease (FMDV 3Cpro) inhibitor, with IC50 values of 0.05 and 0.11 µM in FRET and cell-based assay, respectively .
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-
-
- HY-160276
-
|
Biochemical Assay Reagents
Fluorescent Dye
Liposome
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Others
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DOPE-PEG-Fluor 555, MW 5,000 is consist of a DOPE phospholipid which is an unsaturated phospholipid and a Fluor 555 dye which is a bright orange cyanine dye that can be used in fluorescence microscopy, FRET and other in vivo imaging techniques.
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-
-
- HY-144127
-
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Androgen Receptor
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Cancer
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AR antagonist 3 is a potent and selective androgen receptor (AR) antagonist with an IC50 of 0.47 µM. AR antagonist 3 exhibits a dose-dependent decrease of the FRET signal (IC50= 18.05 μM). AR antagonist 3 shows effective inhibition on tumor growth when administered intratumorally .
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-
-
- HY-P1859
-
-
-
- HY-102075A
-
-
-
- HY-100522
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FMK 9a
2 Publications Verification
|
Autophagy
|
Cancer
|
FMK 9a is an autophagin-1 inhibitor with IC50 values of 80 and 73 μM in FRET and LRA assay.
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-
-
- HY-147366
-
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Others
|
Metabolic Disease
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RBP4 ligand-1 is a non-retinoid ligands for (RBP4) retinol-binding protein 4. The hRBP4SPA IC50 and RBP4–TTR FRET IC50 values are 0.23±0.11 and 0.13±0.12 μM in the radioligand binding assay and FRET assay, respectively .
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-
-
- HY-D1682
-
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Fluorescent Dye
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Others
|
NFQ1 is a non-fluorescent quencher (absorption wavelength: 474 nm), and is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method. OS-FRET enable measurement of unquenched donor emission in the same sample. NFQ1 shows broad absorption spectrum ensuring its utility as a dark acceptor for many donors .
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-
-
- HY-D1046
-
Dabcyl, SE
|
Fluorescent Dye
|
Metabolic Disease
|
Dabcyl acid, SE is the amino-reactive form of Dabcyl acid (DABCYL), and widely used to prepare a variety of FRET-based probes that contain DABCYL.
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-
-
- HY-101533A
-
|
Bcl-2 Family
|
Cancer
|
AZD-5991 Racemate is the racemate of AZD-5991. AZD-5991 Racemate is a Mcl-1 inhibitor with an IC50 of <3 nM in FRET assay.
|
-
-
- HY-100443B
-
(-)-PX-102 (trans isomer); (-)-PX-104
|
FXR
Autophagy
|
Metabolic Disease
|
(-)-PX20606 trans isomer is a FXR agonist with EC50s of 18 and 29 nM for FXR in FRET and M1H assay, respectively.
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-
-
- HY-125452
-
|
Fluorescent Dye
|
Others
|
DiSBAC10 is a voltage-sensitive fluorescent probe used to study cell membrane electrical activity in FRET assays. In a resting polarized cell, DiSBAC10 resides on the outer leaflet of the membrane where it accepts photons from excited fluorescein-labeled proteins and re-emits the photons at a higher wavelength. Depolarization of the cell causes rapid translocation of DiSBAC10 to the inner leaflet of the membrane, thereby increasing the distance between fluorophores and reducing the FRET signal.
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-
-
- HY-D0053
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6-Carboxy-X-rhodamine
|
Fluorescent Dye
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Others
|
6-ROX, a fluorescent marker of oligonucleotides, acts as a receptor coupled to 5-FAM and as a donor in FRET imaging. Excitation wavelength: 568nm. Emission wavelength: 568nm.
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-
-
- HY-100443A
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trans-PX-102
|
FXR
Autophagy
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Metabolic Disease
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PX20606 trans racemate (PX-102 trans racemate) is a FXR agonist with EC50s of 32 and 34 nM for FXR in FRET and M1H assay, respectively .
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-
-
- HY-101533
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AZD-5991
Maximum Cited Publications
8 Publications Verification
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Bcl-2 Family
|
Cancer
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AZD-5991 is a potent and selective Mcl-1 inhibitor with an IC50 of 0.7 nM in FRET assay and a Kd of 0.17 nM in surface plasmon resonance (SPR) assay .
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-
-
- HY-148643
-
|
STING
|
Inflammation/Immunology
|
STING modulator-5 (compound 38) is a STING modulator with a pIC50 value of 9.5. STING modulator-5 antagonizes peripheral blood mononuclear cells (PBMC) with a pIC50 value of 8.1. STING modulator-5 is a antagonist of THP-1 cells, it can be used for immunological disease research .
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-
-
- HY-D2274
-
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Fluorescent Dye
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Others
|
EZH2-AF647 is a fluorescent probe derived from UNC2239 that improves receptor TR-FRET properties by using Alexa 647 dye .
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-
-
- HY-101027
-
|
|
|
GSK 4027 is a chemical probe for the PCAF/GCN5 bromodomain with an pIC50 of 7.4±0.11 for PCAF in a time-resolved fluorescence resonance energy transfer (TR-FRET) assay.
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-
-
- HY-D0053A
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6-Carboxy-X-rhodamine hydrochloride
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Fluorescent Dye
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Others
|
6-ROX (6-Carboxy-X-rhodamine) hydrochloride, a fluorescent marker of oligonucleotides, acts as a receptor coupled to 5-FAM and as a donor in FRET imaging. Excitation wavelength: 568 nm. Emission wavelength: 568 nm.
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-
-
- HY-149233
-
-
-
- HY-161159
-
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Fluorescent Dye
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Others
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DMHBO+ is a cationic chromophore that binds to the Chili aptamer with a Kd of 12 nM. The Chili-DMHBO+ complex is an ideal fluorescence donor for FRET to the rhodamine dye Atto 590, suitable for imaging RNA in cells. Ex/Em=456/592 nm .
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-
-
- HY-P99900
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MSL-109
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CMV
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Infection
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Sevirumab (MSL-109) is a human IgG1 neutralizing monoclonal antibody against cytomegalovirus (CMV). Sevirumab recognizes CMV gH complexes and inhibits CMV replication with an EC50 of 0.3 μg/mL .
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-
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- HY-D1079
-
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DNA Stain
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Others
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EDANS sodium is a potent fluorogenic substrates. EDANS sodium is a donor for FRET-based nucleic acid probes and protease substrates. EDANS sodium is often paired with DABCYL or DABSYL. The optimal absorbance and emission wavelengths of EDANS sodium are λabs = 336 nm and λem = 490 nm respectively .
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-
-
- HY-P4787
-
|
Cathepsin
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Others
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Ac-Glu-Asp(EDANS)-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Gly-Lys(DABCYL)-Glu-NH2 is a cathepsin D substrate, that can be used for cathepsin D FRET assay .
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-
-
- HY-101027A
-
-
-
- HY-157558
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8SGE
|
Others
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Others
|
KDRLKZ-1 is a KLHDC2 ligand (Kd = 0.36 μM), with IC50s of 0.21 μM and 0.31 μM in alphaLISA and time-resolved fluorescence resonance energy transfer (TR-FRET) displacement assays .
|
-
-
- HY-108435
-
-
-
- HY-101533B
-
|
Bcl-2 Family
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Cancer
|
AZD-5991 S-enantiomer is the less active enantiomer of AZD-5991. AZD-5991 S-enantiomer is a Mcl-1 inhibitor with an IC50 of 6.3 μM in FRET assay and a Kd of 0.98 μM in surface plasmon resonance (SPR) assay.
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-
-
- HY-D1049
-
6-((2,4-Dinitrophenyl)amino)hexanoic acid
|
Fluorescent Dye
|
Metabolic Disease
|
DNP-X acid (6-((2,4-Dinitrophenyl)amino)hexanoic acid), an amine-reactive building block for developing a probe, can be recognized by anti-DNP antibodies. DNP-X acid is also an excellent amine-reactive FRET quencher paired with Trp or Tyr.
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-
-
- HY-135317
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CA-4948
|
IRAK
FLT3
Apoptosis
|
Inflammation/Immunology
Cancer
|
Emavusertib (CA-4948) is a selective, potent and orally active IRAK4/FLT3 inhibitor. Emavusertib has an IC50 of 57 nM for IRAK4 in a FRET kinase assay. Emavusertib shows anti-tumor activity .
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-
-
- HY-135317B
-
CA-4948 hydrochloride
|
IRAK
FLT3
|
Inflammation/Immunology
Cancer
|
Emavusertib (CA-4948) hydrochloride is a selective, potent and orally active IRAK4/FLT3 inhibitor. Emavusertib hydrochloride has an IC50 of 57 nM for IRAK4 in a FRET kinase assay. Emavusertib hydrochloride shows anti-tumor activity .
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-
-
- HY-149245
-
|
Keap1-Nrf2
|
Inflammation/Immunology
|
Keap1-Nrf2-IN-15 (Compound 24a) is a potent Keap1-Nrf2 protein-protein interaction inhibitor with IC50s of 77 nM and 2.5 nM in FP assay and TR-FRET assay, respectively .
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-
-
- HY-139561
-
-
-
- HY-155311
-
|
Others
|
Inflammation/Immunology
|
NKG2D-IN-1 (compound 21) is a natural killer group 2D receptor (NKG2D) inhibitor with IC50s of 0.3 µM and 0.7 µM in NKG2D/MICA and ULBP6 TR-FRET assays, respectively .
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-
-
- HY-155312
-
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Others
|
Cancer
|
NKG2D-IN-2 (compound 47) is a natural killer group 2D receptor (NKG2D) inhibitor with IC50s of 0.1 µM and 0.2 µM in NKG2D/MICA and ULBP6 TR-FRET assays, respectively .
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-
-
- HY-136521
-
|
Epigenetic Reader Domain
|
Cancer
|
AZ13824374 is a highly potent and selective ATAD2 bromodomain inhibitor which shows cellular target engagement and antiproliferative activity in a range of breast cancer models. AZ13824374 inhibits ATAD2 with pIC50s of 8.2 and 6.2 in ATAD2 FRET assay and ATAD2 NanoBRET assay, respectively .
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-
-
- HY-D1050
-
6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester
|
Fluorescent Dye
|
Metabolic Disease
|
DNP-X, SE (6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester), the DNP-X acid modified by succinimidyl ester, is an amine-reactive building block for developing a probe, which can be recognized by anti-DNP antibodies. DNP-X, SE (6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester) is also an excellent amine-reactive FRET quencher paired with Trp or Tyr.
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-
-
- HY-108696
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GNE-781
2 Publications Verification
|
Epigenetic Reader Domain
Histone Acetyltransferase
|
Cancer
|
GNE-781 is an orally active, highly potent and selective CBP inhibitor with an IC50 of 0.94 nM in TR-FRET assay. GNE-781 also inhibits BRET and BRD4(1) with IC50s of 6.2 nM and 5100 nM, respectively. GNE-781 displays antitumor activity in an MOLM-16 AML xenograft model .
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-
-
- HY-153114
-
|
FXR
|
Inflammation/Immunology
|
HEC96719 is a selective and orally active tricyclic farnesoid X receptor (FXR) agonist with EC50 values of 1.37 and 1.55 nM by time-resolved fluorescence energy transfer (TR-FRET) and luciferase reporter assays, respectively. HEC96719 significantly improves non-alcoholic steatohepatitis (NASH) and liver fibrosis with favorable tissue distribution in liver and intestine. HEC96719 can be used for the research of non-alcoholic steatohepatitis .
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-
-
- HY-W027544S
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7-Methoxy-d3-2-oxo-2H-1-benzopyran-4-acetic acid; 7-Methoxy-d3-2H-chromen-2-one-4-acetic acid
|
Isotope-Labeled Compounds
|
Others
|
(7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d3 is the deuterium labeled MCA[1]. MCA is a coumarin derivative. MCA quantitates platelet-activating factor by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities[2][3].
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-
-
- HY-P4919
-
|
Beta-secretase
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Others
|
Mca-SEVNLDAEFK(Dnp) is a Beta-secretase 1 (BACE-1) peptide FRET substrate, containing the 'Swedish' Lys-Met/Asn-Leu mutation of the amyloid precursor protein (APP) β-secretase cleavage site. Cleavage at -Leu-Asp- of Mca-SEVNLDAEFK(Dnp) liberates the highly fluorescent 7-methoxycoumarin (Mca) fragment from the proximity quenching effect of the 2,4-dinitrophenyl (Dnp) internal quencher resulting in a large and easily detectable increase in fluorescence intensity.
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-
-
- HY-146578
-
|
Keap1-Nrf2
|
Inflammation/Immunology
|
Keap1-Nrf2-IN-8 (compound 12d) is a potent Keap1-Nrf2 PPI (Keap1-Nrf2 protein−protein interaction) inhibitor with IC50s of 64.5 nM and 14.2 nM for FP and TR-FRET assays, respectively. Keap1-Nrf2-IN-8 significantly increases the mRNA levels of Nrf2 downstream genes, GSTM3, HMOX2 and NQO1 .
|
-
-
- HY-W027544S1
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5-(Dimethyl-d6-amino)-N-(prop-2-ynyl)naphthalene-1-sulfonamide
|
Isotope-Labeled Compounds
|
Others
|
(7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d6 is the deuterium labeled MCA[1]. MCA is a coumarin derivative. MCA quantitates platelet-activating factor by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities[2][3]. (7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d6 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
|
-
Cat. No. |
Product Name |
Type |
-
- HY-W027544
-
|
Fluorescent Dyes/Probes
|
MCA is a coumarin derivative. MCA quantitates platelet-activating factor by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities .
|
-
- HY-D1046
-
Dabcyl, SE
|
Fluorescent Dyes/Probes
|
Dabcyl acid, SE is the amino-reactive form of Dabcyl acid (DABCYL), and widely used to prepare a variety of FRET-based probes that contain DABCYL.
|
-
- HY-D0053
-
6-Carboxy-X-rhodamine
|
Oligonucleotide Labeling
|
6-ROX, a fluorescent marker of oligonucleotides, acts as a receptor coupled to 5-FAM and as a donor in FRET imaging. Excitation wavelength: 568nm. Emission wavelength: 568nm.
|
-
- HY-D1682
-
|
Fluorescent Dyes/Probes
|
NFQ1 is a non-fluorescent quencher (absorption wavelength: 474 nm), and is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method. OS-FRET enable measurement of unquenched donor emission in the same sample. NFQ1 shows broad absorption spectrum ensuring its utility as a dark acceptor for many donors .
|
-
- HY-125452
-
|
Fluorescent Dyes/Probes
|
DiSBAC10 is a voltage-sensitive fluorescent probe used to study cell membrane electrical activity in FRET assays. In a resting polarized cell, DiSBAC10 resides on the outer leaflet of the membrane where it accepts photons from excited fluorescein-labeled proteins and re-emits the photons at a higher wavelength. Depolarization of the cell causes rapid translocation of DiSBAC10 to the inner leaflet of the membrane, thereby increasing the distance between fluorophores and reducing the FRET signal.
|
-
- HY-D2274
-
|
Fluorescent Dyes/Probes
|
EZH2-AF647 is a fluorescent probe derived from UNC2239 that improves receptor TR-FRET properties by using Alexa 647 dye .
|
-
- HY-D1049
-
6-((2,4-Dinitrophenyl)amino)hexanoic acid
|
Fluorescent Dyes/Probes
|
DNP-X acid (6-((2,4-Dinitrophenyl)amino)hexanoic acid), an amine-reactive building block for developing a probe, can be recognized by anti-DNP antibodies. DNP-X acid is also an excellent amine-reactive FRET quencher paired with Trp or Tyr.
|
-
- HY-D1050
-
6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester
|
Fluorescent Dyes/Probes
|
DNP-X, SE (6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester), the DNP-X acid modified by succinimidyl ester, is an amine-reactive building block for developing a probe, which can be recognized by anti-DNP antibodies. DNP-X, SE (6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester) is also an excellent amine-reactive FRET quencher paired with Trp or Tyr.
|
Cat. No. |
Product Name |
Type |
-
- HY-160276
-
|
Drug Delivery
|
DOPE-PEG-Fluor 555, MW 5,000 is consist of a DOPE phospholipid which is an unsaturated phospholipid and a Fluor 555 dye which is a bright orange cyanine dye that can be used in fluorescence microscopy, FRET and other in vivo imaging techniques.
|
Cat. No. |
Product Name |
Target |
Research Area |
-
- HY-P2498A
-
|
Cathepsin
|
Cancer
|
Cathepsin D and E FRET Substrate acetate is a fluorogenic substrate for cathepsins D and E and not for B, H or L. The cleavage occurs at the Phe-Phe amide bond resul. Cathepsin D and E FRET Substrate is a valuable tool for routine assays and for mechanistic studies on cathepsins E and D .
|
-
- HY-P5354
-
|
Peptides
|
Others
|
FRETS-VWF73 is a biological active peptide. (FRETS-VWF73 is a very powerful Fluorescence-Quenching Substrate for the VWF cleaving protease ADAMTS-13. It is commonly used as a useful tool for the rapid measurement of ADAMTS-13 activity in plasma and is a predictive marker for various thrombotic diseases like TPP. Our new substrate FRETS-VWF73 can be easily used with a 96-well format in commercial plate readers.)
|
-
- HY-P2492
-
|
Peptides
|
Metabolic Disease
|
Renin FRET Substrate I is a substrate of human renin. Renin FRET Substrate I is designed to incorporate the renin cleavage site that occurs in the N-terminal peptide of human angiotensinogen .
|
-
- HY-P2498
-
|
Cathepsin
|
Others
|
Cathepsin D and E FRET Substrate is a fluorogenic substrate for cathepsins D and E and not for B, H or L. The cleavage occurs at the Phe-Phe amide bond resul. Cathepsin D and E FRET Substrate is a valuable tool for routine assays and for mechanistic studies on cathepsins E and D .
|
-
- HY-P5970
-
TACE Substrate
|
Peptides
|
Cancer
|
DABCYL-TNF-α-EDANS (-4 to +6) (human) is a FRET peptide substrate of tumor necrosis factor convertase (TACE) .
|
-
- HY-P3792
-
|
Peptides
|
Others
|
Mca-Pro-Leu-Gly-Pro-D-Lys(Dnp) is a FRET substrate of Thimet oligopeptidase. Mca-Pro-Leu-Gly-Pro-D-Lys(Dnp) can be used for the determination of Thimet oligopeptidase activity .
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-
- HY-P1859
-
-
- HY-P10196
-
|
Peptides
|
Others
|
DABCYL-SEVNLDAEF-EDANS is a substrate of FRET. DABCYL-SEVNLDAEF-EDANS can be used in the BACE activity assay .
|
-
- HY-P4787
-
|
Cathepsin
|
Others
|
Ac-Glu-Asp(EDANS)-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Gly-Lys(DABCYL)-Glu-NH2 is a cathepsin D substrate, that can be used for cathepsin D FRET assay .
|
-
- HY-P5323
-
|
Peptides
|
Others
|
Dabcyl-AGHDAHASET-Edans is a biological active peptide. (This is a type I signal peptidase (SPase1) substrate peptide labeled with EDANS/ DABCYL FRET pair, and contains a crucial cleavage site derived from the C-terminal region of the Staphylococcus epidermidis pre-SceD protein. Abs/Em = 340/490 nm.)
|
-
- HY-P5419
-
|
Peptides
|
Others
|
WAAG-3R is a biological active peptide. (Aggrecanases belong to the ADAMTS (A disintegrin and metalloprotease with thrombospondin motif) family of proteases. Aggrecanases cleave aggrecan, the major structural component of cartilage. Aggrecanase-1 (ADAMTS-4) is a major aggrecanase in human osteoarthritic cartilage. This FRET peptide was used in an ADAMTS-4 (Aggrecanase-1) assay. Ex/Em = 340/420 nm.)
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-
- HY-P5344
-
Fluorigenic PEXEL peptide
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Peptides
|
Others
|
Dabcyl-LNKRLLHETQ-Edans (Fluorigenic PEXEL peptide) is a biological active peptide. (This FRET substrate peptide for Plasmepsin V (PMV) is derived from the conserved Plasmodium Export Element (PEXEL) motif of Histidine-Rich Protein II (HRPII). PMV is an ER aspartic protease that recognizes and cleaves the RXL sequence within the PEXEL motif of proteins exported by human malaria parasite Plasmodium falciparum, allowing them to translocate into host erythrocytes.)
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-
- HY-P5419A
-
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Peptides
|
Others
|
WAAG-3R TFA is a biological active peptide. (Aggrecanases belong to the ADAMTS (A disintegrin and metalloprotease with thrombospondin motif) family of proteases. Aggrecanases cleave aggrecan, the major structural component of cartilage. Aggrecanase-1 (ADAMTS-4) is a major aggrecanase in human osteoarthritic cartilage. This FRET peptide was used in an ADAMTS-4 (Aggrecanase-1) assay. Ex/Em = 340/420 nm.) .
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-
- HY-P4919
-
|
Beta-secretase
|
Others
|
Mca-SEVNLDAEFK(Dnp) is a Beta-secretase 1 (BACE-1) peptide FRET substrate, containing the 'Swedish' Lys-Met/Asn-Leu mutation of the amyloid precursor protein (APP) β-secretase cleavage site. Cleavage at -Leu-Asp- of Mca-SEVNLDAEFK(Dnp) liberates the highly fluorescent 7-methoxycoumarin (Mca) fragment from the proximity quenching effect of the 2,4-dinitrophenyl (Dnp) internal quencher resulting in a large and easily detectable increase in fluorescence intensity.
|
-
- HY-P5481
-
|
Peptides
|
Others
|
DABCYL-LPETG-EDANS is a biological active peptide. (This 5-amino acid peptide is a sortase substrate, C-terminal sorting signal. Sortase cleaves surface proteins at the LPXTG motif and catalyzes the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Sortases are a family of Gram-positive transpeptidases responsible for anchoring surface protein virulence factors to the peptidoglycan cell wall layer. Cleavage of this FRET substrate by sortase reveals the fluorescent signal, Abs/Em = 340/490 nm.)
|
-
- HY-P5528
-
|
Peptides
|
Others
|
2Abz-SLGRKIQIK(Dnp)-NH2 is a biological active peptide. (This peptide is a second complement component (C2), the physiological substrate for the proenzyme Cls, first complement component. The complement system is a central component of host defense but can also contribute to the inflammation seen in pathological conditions. The C1s protease of the C1 complex initiates the host defense pathway. This peptide employs 2Abz/Dnp FRET pair for quantitation of complement enzyme activity.)
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-
- HY-P5415
-
|
Peptides
|
Others
|
DABCYL-GABA-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS is a biological active peptide. (DABCYL-GABA-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS is also called HIV protease substrate I in some literature. It is widely used for the continuous assay for HIV protease activity. The 11-kD protease (PR) encoded by the human immunodeficiency virus 1 (HIV-1) is essential for the correct processing of viral polyproteins and the maturation of infectious virus, and is therefore a target for the design of selective acquired immunodeficiency syndrome (AIDS) therapeutics. The FRET-based fluorogenic substrate is derived from a natural processing site for HIV-1 PR. Incubation of recombinant HIV-1 PR with the fluorogenic substrate resulted in specific cleavage at the Tyr-Pro bond and a time-dependent increase in fluorescence intensity that is linearly related to the extent of substrate hydrolysis. The fluorescence quantum yields of the HIV-1 PR substrate in the FRET assay increased by 40.0- and 34.4-fold, respectively, per mole of substrate cleaved. Because of its simplicity and precision in the determination of reaction rates required for kinetic analysis, this substrate offers many advantages over the commonly used HPLC or electrophoresis-based assays for peptide substrate hydrolysis by retroviral PRs. Abs/Em = 340nm/490nm.)
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-
- HY-P5377
-
Cathepsin K substrate
|
Peptides
|
Others
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Abz-HPGGPQ-EDDnp (Cathepsin K substrate) is a biological active peptide. (Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin K is the lysosomal cysteine protease involved in bone remodeling and resorption. It has potential as a drug target in autoimmune diseases and osteoporosis.This FRET peptide can be used to monitor selectively cathepsin K activity in physiological fluids and cell lysates. Abz-HPGGPQ-EDDnp [where Abz represents o-aminobenzoic acid and EDDnp represents N -(2, 4-dinitrophenyl)-ethylenediamine], a substrate initially developed for trypanosomal enzymes, is efficiently cleaved at the Gly-Gly bond by cathepsin K. This peptide is resistant to hydrolysis by cathepsins B, F, H, L, S and V, Ex/Em=340 nm/420 nm.)
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Cat. No. |
Product Name |
Target |
Research Area |
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- HY-P99900
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MSL-109
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CMV
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Infection
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Sevirumab (MSL-109) is a human IgG1 neutralizing monoclonal antibody against cytomegalovirus (CMV). Sevirumab recognizes CMV gH complexes and inhibits CMV replication with an EC50 of 0.3 μg/mL .
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Cat. No. |
Product Name |
Chemical Structure |
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- HY-W027544S
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(7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d3 is the deuterium labeled MCA[1]. MCA is a coumarin derivative. MCA quantitates platelet-activating factor by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities[2][3].
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- HY-W027544S1
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(7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d6 is the deuterium labeled MCA[1]. MCA is a coumarin derivative. MCA quantitates platelet-activating factor by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities[2][3]. (7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d6 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
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Cat. No. |
Product Name |
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Classification |
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- HY-W027544S1
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5-(Dimethyl-d6-amino)-N-(prop-2-ynyl)naphthalene-1-sulfonamide
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Alkynes
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(7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d6 is the deuterium labeled MCA[1]. MCA is a coumarin derivative. MCA quantitates platelet-activating factor by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities[2][3]. (7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d6 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
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