NFQ1

Cat. No.: HY-D1682: FAQs
Data Sheet Handling Instructions

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NFQ1 is a non-fluorescent quencher (absorption wavelength: 474 nm), and is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method. OS-FRET enable measurement of unquenched donor emission in the same sample. NFQ1 shows broad absorption spectrum ensuring its utility as a dark acceptor for many donors.

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NFQ1 Chemical Structure

CAS No. : 1262391-07-0

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Description	NFQ1 is a non-fluorescent quencher (absorption wavelength: 474 nm), and is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method. OS-FRET enable measurement of unquenched donor emission in the same sample. NFQ1 shows broad absorption spectrum ensuring its utility as a dark acceptor for many donors^[1].
In Vitro	NFQ1 is used for One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method employing a novel, non-fluorescent methanethiosulfonate-linked acceptor that can be reversibly coupled to a target sulfhydryl residue via a disulfide bond. After the quenched donor emission is quantitated the acceptor is removed by reduction, enabling measurement of unquenched donor emission in the same sample^[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. NFQ1 Related Antibodies
Molecular Weight	451.48
Formula	C₁₇H₁₇N₅O₆S₂
CAS No.	1262391-07-0
Emission (Em)	525
Excitation (Ex)	474
SMILES	CS(=O)(SCCNC(CC1=CC=C(C=C1)NC2=CC=C(C3=NON=C23)[N+]([O-])=O)=O)=O
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation	Data Sheet (266 KB) Handling Instructions (2659 KB)
References	[1]. Annette H Erbse, et al. OS-FRET: a new one-sample method for improved FRET measurements. Biochemistry. 2011 Feb 1;50(4):451-7. [Content Brief]

NFQ1 Related Classifications

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NFQ11262391-07-0NFQ 1NFQ-1Fluorescent DyeOne Sample Fluorescence Resonance Energy TransferOS-FRETunquenched donor emissiondark acceptorInhibitorinhibitorinhibit

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NFQ1

NFQ1 Related Antibodies

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