NKG2D-IN-1
Based on 1 Customer Validation
NKG2D-IN-1 (compound 21) is a natural killer group 2D receptor (NKG2D) inhibitor. NKG2D-IN-1 inhibits the protein-protein interaction between NKG2D and its ligands MICA and ULBP6, with IC50 values of 0.3 μM and 0.7 μM. NKG2D-IN-1 can be used in the research of autoimmune diseases.
For research use only. We do not sell to patients.
- Purity: 99.84%
- Formula: C29H24F6N4O2
- Molecular Weight:574.52
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
NKG2D-IN-1 (compound 21) inhibits cell-based NKG2D/MICA and NKG2D/ULBP6 interactions with IC50 values of 0.3 μM and 0.7 μM, respectively, and forms a van der Waals interaction with Lys150B of human NKG2D[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 574.52
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Formula C29H24F6N4O2
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Color White to off-white
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SMILES
CN1N=CC=C1C2=CC(C(NC(C(N(C)C)=O)C3=CC=CC(C(F)(F)F)=C3)=O)=C(C4=CC=C(C(F)(F)F)C=C4)C=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (174.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (8.70 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.7406 mL | 8.7029 mL | 17.4058 mL | 43.5146 mL |
| 5 mM | 0.3481 mL | 1.7406 mL | 3.4812 mL | 8.7029 mL | |
| 10 mM | 0.1741 mL | 0.8703 mL | 1.7406 mL | 4.3515 mL | |
| 15 mM | 0.1160 mL | 0.5802 mL | 1.1604 mL | 2.9010 mL | |
| 20 mM | 0.0870 mL | 0.4351 mL | 0.8703 mL | 2.1757 mL | |
| 25 mM | 0.0696 mL | 0.3481 mL | 0.6962 mL | 1.7406 mL | |
| 30 mM | 0.0580 mL | 0.2901 mL | 0.5802 mL | 1.4505 mL | |
| 40 mM | 0.0435 mL | 0.2176 mL | 0.4351 mL | 1.0879 mL | |
| 50 mM | 0.0348 mL | 0.1741 mL | 0.3481 mL | 0.8703 mL | |
| 60 mM | 0.0290 mL | 0.1450 mL | 0.2901 mL | 0.7252 mL | |
| 80 mM | 0.0218 mL | 0.1088 mL | 0.2176 mL | 0.5439 mL | |
| 100 mM | 0.0174 mL | 0.0870 mL | 0.1741 mL | 0.4351 mL |
NKG2D-IN-1 Related Classifications
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