2. PROTAC Epigenetics
  3. PROTACs YTHDF
  4. SK-3-91

SK-3-91 is a PROTAC-type multi-kinase degrader that can jointly induce the degradation of the largest number of unique kinases (more than 125 unique kinases). SK-3-91 induces protein (YTHDF2, e.g.) degradation through the ubiquitin biotinylation (E-STUB) pathway. SK-3-91 degrades YTHDF2. SK-3-91 inhibits cell proliferation and induces morphological changes.
(Pink: YTHDF2 ligand (HY-169396); Blue: Cereblon ligand (HY-131717); Black: linker (HY-140819)).

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SK-3-91

SK-3-91 構造式

CAS 番号 : 2769753-23-1

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 937 在庫あり
Solution
10 mM * 1 mL in DMSO USD 937 在庫あり
Solid
5 mg $480 在庫あり
10 mg $800 在庫あり
25 mg $1550 在庫あり
50 mg $2400 在庫あり
100 mg $3600 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 0 publication(s) in Google Scholar

SK-3-91 関連抗体

Top Publications Citing Use of Products

PROTACs アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
von Hippel-Lindau (VHL) Cereblon MDM2 cIAP1

YTHDF アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
YTHDF1 YTHDF3 YTHDF2 YTHDC1 YTHDC2

  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

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製品説明

SK-3-91 is a PROTAC-type multi-kinase degrader that can jointly induce the degradation of the largest number of unique kinases (more than 125 unique kinases). SK-3-91 induces protein (YTHDF2, e.g.) degradation through the ubiquitin biotinylation (E-STUB) pathway. SK-3-91 degrades YTHDF2. SK-3-91 inhibits cell proliferation and induces morphological changes[1][2]. (Pink: YTHDF2 ligand (HY-169396); Blue: Cereblon ligand (HY-131717); Black: linker (HY-140819)).

IC50 & Target

YTHDF2

 

体外実験

SK-3-91 (0.12-10 μM, 24 h) degrades YTHDF2 at the sub-micromolar concentrations in K562 cells[2].
SK-3-91 (1-50 μM) shows significantly less cytotoxicity against the U2OS cells at the higher concentrations of either 3 μM or 10 μM[2].
SK-3-91 (400 nM, 72 h) inhibits cell proliferation in K562 cells[2].
SK-3-91(1-10 μM, 20 h) induces morphological changes in U2OS cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SK-3-91 関連抗体

Cell Proliferation Assay[2]

Cell Line: K562 cells
Concentration: 400 nM
Incubation Time: 72 h
Result: Showed inhibition of 88.2%.

Western Blot Analysis[2]

Cell Line: K562 cells
Concentration: 0.12, 0.37, 1.1, 3.3, 10 μM
Incubation Time: 24 h
Result: Degraded YTHDF2 at the sub-micromolar concentrations.
分子量

887.40

分子式

C42H47ClN10O8S
CAS 番号
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(N1C2C(NC(CC2)=O)=O)C3=C(NCC(NCCOCCN4CCN(C5=CC=C(NC6=NC(NC7=C(S(C(C)C)(=O)=O)C=CC=C7)=C(Cl)C=N6)C=C5)CC4)=O)C=CC=C3C1=O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
体外: 

DMSO : 200 mg/mL (225.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1269 mL 5.6344 mL 11.2689 mL
5 mM 0.2254 mL 1.1269 mL 2.2538 mL
10 mM 0.1127 mL 0.5634 mL 1.1269 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 5 mg/mL (5.63 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 5 mg/mL (5.63 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 98.33%

COA (245 KB) HNMR (256 KB) LCMS (88 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1269 mL 5.6344 mL 11.2689 mL 28.1722 mL
5 mM 0.2254 mL 1.1269 mL 2.2538 mL 5.6344 mL
10 mM 0.1127 mL 0.5634 mL 1.1269 mL 2.8172 mL
15 mM 0.0751 mL 0.3756 mL 0.7513 mL 1.8781 mL
20 mM 0.0563 mL 0.2817 mL 0.5634 mL 1.4086 mL
25 mM 0.0451 mL 0.2254 mL 0.4508 mL 1.1269 mL
30 mM 0.0376 mL 0.1878 mL 0.3756 mL 0.9391 mL
40 mM 0.0282 mL 0.1409 mL 0.2817 mL 0.7043 mL
50 mM 0.0225 mL 0.1127 mL 0.2254 mL 0.5634 mL
60 mM 0.0188 mL 0.0939 mL 0.1878 mL 0.4695 mL
80 mM 0.0141 mL 0.0704 mL 0.1409 mL 0.3522 mL
100 mM 0.0113 mL 0.0563 mL 0.1127 mL 0.2817 mL
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SK-3-91 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SK-3-912769753-23-1PROTACsYTHDF(YTH) domain-containing family proteins (YTHDF)unique kinasesMOLT-4 cellsDB0646SB1-G-187 cellsInhibitorinhibitorinhibit

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製品名:
SK-3-91
製品番号:
HY-137341
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