2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. TLR4-IN-C34

TLR4-IN-C34 is an orally active TLR4 inhibitor and reduces systemic inflammation in models of endotoxemia and necrotizing enterocolitis.

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CAS 番号 : 40592-88-9

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10 mM * 1 mL in DMSO
ready for reconstitution
 USD 84 在庫あり
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10 mM * 1 mL in DMSO USD 84 在庫あり
Solid
5 mg $77 在庫あり
10 mg $110 在庫あり
25 mg $176 在庫あり
50 mg $286 在庫あり
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カスタマーレビュー

Based on 14 publication(s) in Google Scholar

Other Forms of TLR4-IN-C34:

TLR4-IN-C34 関連抗体

Top Publications Citing Use of Products

    TLR4-IN-C34 purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2025 Dec;17(1):2471015.  [Abstract]

    TLR4-IN-C34 ( orally; 1 mg/kg daily from the 7th postnatal day to the 3rd day after HI insult) treatment improved intestinal dysfunction and alleviated synaptic and cognitive impairments in the neonatal HIBD rats. The expression levels of TLR4 of colon by WB and IF analyses.

    TLR4-IN-C34 purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Aug 26;23(1):381.  [Abstract]

    Neutrophils were pretreated with the TLR2 and TLR4 inhibitor Sparstolonin B (SsnB) (HY-116213; 10 µM), the TLR2 inhibitor C29 (HY-100461; 100 µM), or the TLR4 inhibitor C34 (TLR4-IN-C34; HY-107575; 10 µM) for 30 min before stimulation with calreticulin. Transwell migration assay. The lower chambers were loaded with blank culture medium (a), calreticulin (b), calreticulin with SsnB (c), calreticulin with C29 (d), or calreticulin with C34 (e). CFSE-labeled neutrophils were loaded into the upper chamber and allowed to migrate for 3 h.

    TLR4-IN-C34 purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Aug 26;23(1):381.  [Abstract]

    Neutrophils were pretreated with the TLR2 and TLR4 inhibitor Sparstolonin B (SsnB) (HY-116213; 10 µM), the TLR2 inhibitor C29 (HY-100461; 100 µM), or the TLR4 inhibitor C34 (TLR4-IN-C34; HY-107575; 10 µM) for 30 min before stimulation with calreticulin. F-actin was detected for 30 min the incubation of neutrophils with calreticulin in the presence of the respective inhibitors (scale bars = 10 μm).

    TLR4-IN-C34 purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Aug 26;23(1):381.  [Abstract]

    Neutrophils were incubated in calreticulin with or without inhibitors of TLR2/TLR4 (SsnB; HY-116213; 10 µM), TLR2 (C29; HY-100461; 100 µM), or TLR4 (C34; TLR4-IN-C34; HY-107575; 10 µM) for 0 min, 1 min, 3 min, or 5 min. The cell lysate was prepared and subjected to western blotting for analysis of the expression of Cdc42, Rac1, and RhoA. Densitometric analysis of each protein band is shown below the blots, representing grayscale quantification relative to internal controls

    TLR4-IN-C34 purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2023 May 1;21(1):86.  [Abstract]

    Neutrophils isolated from healthy individuals were pretreated with inhibitors of TLR2 (HY-112146; MMG-11), TLR4 (HY-107575; TLR4-IN-C34 ), TLR9 (HY-131952; TLR7/8/9-IN-1) and RAGE (HY-P2268; RAGE antagonist peptide) for 1 h prior to coculture with hypoxic-CM from GC cells, and the p-p38 level in neutrophils was measured by western blotting.

    TLR4-IN-C34 purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2023 May 1;21(1):86.  [Abstract]

    Neutrophils isolated from healthy individuals were pretreated with inhibitors of TLR2 (HY-112146; MMG-11), TLR4 (HY-107575; TLR4-IN-C34 ), TLR9 (HY-131952; TLR7/8/9-IN-1) and RAGE (HY-P2268; RAGE antagonist peptide) for 1 h prior to coculture with hypoxic-CM from GC cells, and the NET formation was detected with MPO and citH3 staining. Magnification: 20 × . Scale bars: 50 μm. All values are the means ± SDs. ns = not significant, **P < 0.01

    Toll-like Receptor (TLR) アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

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    製品説明

    TLR4-IN-C34 is an orally active TLR4 inhibitor and reduces systemic inflammation in models of endotoxemia and necrotizing enterocolitis[1][2].

    IC50 & Target

    TLR4

     

    体内実験

    TLR4-IN-C34 (C34, 1 mg/kg, orally daily) could attenuate NEC severity and demonstrates a marked preservation of the intestinal mucosa[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Experimental NEC induced in 7-8 day old mice[1].
    Dosage: 1 mg/kg.
    Administration: Orally every morning.
    Result: Attenuated intestinal inflammation.
    分子量

    389.40

    分子式

    C17H27NO9
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(N[C@H]([C@@H](OC(C)=O)[C@H](OC(C)=O)[C@@H](COC(C)=O)O1)[C@H]1OC(C)C)C
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 200 mg/mL (513.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 33.33 mg/mL (85.59 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5681 mL 12.8403 mL 25.6805 mL
    5 mM 0.5136 mL 2.5681 mL 5.1361 mL
    10 mM 0.2568 mL 1.2840 mL 2.5681 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 5 mg/mL (12.84 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 5 mg/mL (12.84 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 7.69 mg/mL (19.75 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.94%

    COA (256 KB) HNMR (258 KB) RP-HPLC (244 KB) MS (69 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.5681 mL 12.8403 mL 25.6805 mL 64.2013 mL
    5 mM 0.5136 mL 2.5681 mL 5.1361 mL 12.8403 mL
    10 mM 0.2568 mL 1.2840 mL 2.5681 mL 6.4201 mL
    15 mM 0.1712 mL 0.8560 mL 1.7120 mL 4.2801 mL
    20 mM 0.1284 mL 0.6420 mL 1.2840 mL 3.2101 mL
    25 mM 0.1027 mL 0.5136 mL 1.0272 mL 2.5681 mL
    30 mM 0.0856 mL 0.4280 mL 0.8560 mL 2.1400 mL
    40 mM 0.0642 mL 0.3210 mL 0.6420 mL 1.6050 mL
    50 mM 0.0514 mL 0.2568 mL 0.5136 mL 1.2840 mL
    60 mM 0.0428 mL 0.2140 mL 0.4280 mL 1.0700 mL
    80 mM 0.0321 mL 0.1605 mL 0.3210 mL 0.8025 mL
    DMSO 100 mM 0.0257 mL 0.1284 mL 0.2568 mL 0.6420 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    TLR4-IN-C34 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    TLR4-IN-C3440592-88-9Toll-like Receptor (TLR)Inhibitorinhibitorinhibit

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    製品名:
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    製品番号:
    HY-107575
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