1. Metabolic Enzyme/Protease Apoptosis
  2. Endogenous Metabolite Ferroptosis
  3. trans-3-Indoleacrylic acid

Trans-3-Indoleacrylic acid is a tryptophan metabolite, which promotes tumor development through inhibition of RSL3 (HY-100218A) induced ferroptosis via AHR-ALDH1A3-FSP1-CoQ10 axis, and facilitates colorectal carcinogenesis

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trans-3-Indoleacrylic acid Chemical Structure

trans-3-Indoleacrylic acid Chemical Structure

CAS No. : 29953-71-7

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Based on 1 publication(s) in Google Scholar

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Description

Trans-3-Indoleacrylic acid is a tryptophan metabolite, which promotes tumor development through inhibition of RSL3 (HY-100218A) induced ferroptosis via AHR-ALDH1A3-FSP1-CoQ10 axis, and facilitates colorectal carcinogenesis[1]

IC50 & Target

Microbial Metabolite

 

In Vitro

Trans-3-Indoleacrylic acid activites AHR for increased expression of ALDH1A3, which generates NADH for FSP1-mediated synthesis of coenzyme Q10[1].
Trans-3-Indoleacrylic acid inhibits RSL3 induced ferroptosis in cells HT1080 and 786-O, (50 μM, 24 h), decreases lipids peroxidation (50 μM, 4 h)[1].
Trans-3-Indoleacrylic acid inhibits IKE-induced (10 μM) ferroptosis in HT29 tumor spheroids (100 μM, 48 h ) and HT29 organoids (100 μM, 72 h ) and promotes tumor growth[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: HT29, HT1080
Concentration: 5-500 μM
Incubation Time: 24 h
Result: Inhibited ferroptosis in HT29 and HT1080, with presence of AHR or FSP1.
In Vivo

Trans-3-Indoleacrylic acid (50 mg/kg, i.p., three times a week) promotes tumor development in HT29 tumor-bearing xenograft nu/nu mice [1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: HT29 tumor-bearing xenograft nu/nu mice[1]
Dosage: 50 mg/kg
Administration: Intraperitoneal injection, three times a week
Result: Promoted tumor growth with deficiency of AHR or FSP1.
Molecular Weight

187.19

Formula

C11H9NO2

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(/C=C/C1=CNC2=C1C=CC=C2)O.[E]

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (1335.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.3422 mL 26.7108 mL 53.4217 mL
5 mM 1.0684 mL 5.3422 mL 10.6843 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (11.11 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (11.11 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.45%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.3422 mL 26.7108 mL 53.4217 mL 133.5541 mL
5 mM 1.0684 mL 5.3422 mL 10.6843 mL 26.7108 mL
10 mM 0.5342 mL 2.6711 mL 5.3422 mL 13.3554 mL
15 mM 0.3561 mL 1.7807 mL 3.5614 mL 8.9036 mL
20 mM 0.2671 mL 1.3355 mL 2.6711 mL 6.6777 mL
25 mM 0.2137 mL 1.0684 mL 2.1369 mL 5.3422 mL
30 mM 0.1781 mL 0.8904 mL 1.7807 mL 4.4518 mL
40 mM 0.1336 mL 0.6678 mL 1.3355 mL 3.3389 mL
50 mM 0.1068 mL 0.5342 mL 1.0684 mL 2.6711 mL
60 mM 0.0890 mL 0.4452 mL 0.8904 mL 2.2259 mL
80 mM 0.0668 mL 0.3339 mL 0.6678 mL 1.6694 mL
100 mM 0.0534 mL 0.2671 mL 0.5342 mL 1.3355 mL
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trans-3-Indoleacrylic acid Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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trans-3-Indoleacrylic acid
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