5-Methylcytosine-d₄

製品番号: HY-W008091S 純度: 99%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

5-Methylcytosine-d₄ is the deuterium labeled 5-Methylcytosine (HY-W008091). 5-Methylcytosine is a well-characterized DNA modification in prokaryotes and eukaryotes. 5-Methylcytosine forms symmetrical methylation on CpG dinucleotides in DNA, stabilizes tRNA/rRNA structure in RNA, and affects mRNA translation. 5-Methylcytosine can be oxidized to generate 5hmC, 5fC, and 5caC. 5-Methylcytosine can be used in epigenetics, developmental biology, and the study of diseases such as colorectal cancer and hepatocellular carcinoma.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

5-Methylcytosine-d₄ 構造式

CAS 番号 : 1219795-15-9

容量	価格（税別）	在庫状況	数量
1 mg	$400	在庫あり	Estimated Time of Arrival: December 31
5 mg	$1100	在庫あり	Estimated Time of Arrival: December 31
10 mg		お問い合わせ
50 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

Other Forms of 5-Methylcytosine-d₄:

5-Methylcytosine 在庫あり

DNA/RNA Synthesis アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Human Endogenous Metabolite Microbial Metabolite

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

Application

1. This compound can be used as a tracer.
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.

体外実験

Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

129.15

分子式

C₅H₃D₄N₃O

CAS 番号

1219795-15-9

Unlabeled CAS

554-01-8

Appearance

Solid

Color

White to off-white

SMILES

NC1=C(C([2H])=NC(N1)=O)C([2H])([2H])[2H]

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

H₂O : 7.14 mg/mL (55.28 mM; Need ultrasonic)

DMSO : 5 mg/mL (38.71 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	7.7429 mL	38.7147 mL	77.4294 mL
5 mM	1.5486 mL	7.7429 mL	15.4859 mL
10 mM	0.7743 mL	3.8715 mL	7.7429 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

純度とドキュメンテーション

純度: 99.72%

Select Batch:

データシート (277 KB) SDS (393 KB)

COA (254 KB) HNMR (310 KB) RP-HPLC (247 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Cui X, et al. 5-Methylcytosine RNA Methylation in Arabidopsis Thaliana. Mol Plant. 2017 Nov 6;10(11):1387-1399. [Content Brief]

[2]. Breiling A, et al. Epigenetic regulatory functions of DNA modifications: 5-methylcytosine and beyond. Epigenetics Chromatin. 2015 Jul 21;8:24. [Content Brief]

[3]. Edelheit S, et al. Transcriptome-wide mapping of 5-methylcytidine RNA modifications in bacteria, archaea, and yeast reveals m5C within archaeal mRNAs. PLoS Genet. 2013 Jun;9(6):e1003602. [Content Brief]

[4]. Huang W, et al. Formation and determination of the oxidation products of 5-methylcytosine in RNA. Chem Sci. 2016 Aug 1;7(8):5495-5502. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	7.7429 mL	38.7147 mL	77.4293 mL	193.5734 mL
	5 mM	1.5486 mL	7.7429 mL	15.4859 mL	38.7147 mL
	10 mM	0.7743 mL	3.8715 mL	7.7429 mL	19.3573 mL
	15 mM	0.5162 mL	2.5810 mL	5.1620 mL	12.9049 mL
	20 mM	0.3871 mL	1.9357 mL	3.8715 mL	9.6787 mL
	25 mM	0.3097 mL	1.5486 mL	3.0972 mL	7.7429 mL
	30 mM	0.2581 mL	1.2905 mL	2.5810 mL	6.4524 mL
H₂O	40 mM	0.1936 mL	0.9679 mL	1.9357 mL	4.8393 mL
H₂O	50 mM	0.1549 mL	0.7743 mL	1.5486 mL	3.8715 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.