1. Cell Cycle/DNA Damage
    Metabolic Enzyme/Protease
  2. DNA/RNA Synthesis
    Endogenous Metabolite
  3. 5-Methylcytosine

5-Methylcytosine 

Cat. No.: HY-W008091 Purity: 99.82%
Handling Instructions

5-Methylcytosine is a well-characterized DNA modification, and is also predominantly in abundant non-coding RNAs in both prokaryotes and eukaryotes. 5-Methylcytosine in mRNA is a new epitranscriptome marker inArabidopsis, and that regulation of this modification is an integral part of gene regulatory networks underlying plant development.

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5-Methylcytosine Chemical Structure

5-Methylcytosine Chemical Structure

CAS No. : 554-01-8

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Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

5-Methylcytosine is a well-characterized DNA modification, and is also predominantly in abundant non-coding RNAs in both prokaryotes and eukaryotes. 5-Methylcytosine in mRNA is a new epitranscriptome marker inArabidopsis, and that regulation of this modification is an integral part of gene regulatory networks underlying plant development[1].

IC50 & Target

Human Endogenous Metabolite

 

Molecular Weight

125.13

Formula

C₅H₇N₃O

CAS No.

554-01-8

SMILES

O=C1N=CC(C)=C(N)N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 8.33 mg/mL (66.57 mM; Need ultrasonic)

H2O : 7.14 mg/mL (57.06 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 7.9917 mL 39.9584 mL 79.9169 mL
5 mM 1.5983 mL 7.9917 mL 15.9834 mL
10 mM 0.7992 mL 3.9958 mL 7.9917 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 0.83 mg/mL (6.63 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 0.83 mg/mL (6.63 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 0.83 mg/mL (6.63 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: 99.82%

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  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

5-MethylcytosineDNA/RNA SynthesisEndogenous MetaboliteInhibitorinhibitorinhibit

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Product Name:
5-Methylcytosine
Cat. No.:
HY-W008091
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