5-Methylcytosine-d4
Based on 1 Customer Validation
5-Methylcytosine-d4 is the deuterium labeled 5-Methylcytosine (HY-W008091). 5-Methylcytosine is a well-characterized DNA modification in prokaryotes and eukaryotes. 5-Methylcytosine forms symmetrical methylation on CpG dinucleotides in DNA, stabilizes tRNA/rRNA structure in RNA, and affects mRNA translation. 5-Methylcytosine can be oxidized to generate 5hmC, 5fC, and 5caC. 5-Methylcytosine can be used in epigenetics, developmental biology, and the study of diseases such as colorectal cancer and hepatocellular carcinoma.
For research use only. We do not sell to patients.
- Purity: 99%
- CAS No.: 1219795-15-9
- Formula: C5H3D4N3O
- Molecular Weight:129.15
-
Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
Chemical Information
-
CAS No. 1219795-15-9
-
Unlabeled Cas 554-01-8
-
Appearance Solid
-
Molecular Weight 129.15
-
Formula C5H3D4N3O
-
Color White to off-white
-
SMILES
NC1=C(C([2H])=NC(N1)=O)C([2H])([2H])[2H]
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
H2O : 7.14 mg/mL (55.28 mM; Need ultrasonic)
DMSO : 5 mg/mL (38.71 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (277 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. Cui X, et al. 5-Methylcytosine RNA Methylation in Arabidopsis Thaliana. Mol Plant. 2017 Nov 6;10(11):1387-1399. [Content Brief]
[2]. Breiling A, et al. Epigenetic regulatory functions of DNA modifications: 5-methylcytosine and beyond. Epigenetics Chromatin. 2015 Jul 21;8:24. [Content Brief]
[3]. Edelheit S, et al. Transcriptome-wide mapping of 5-methylcytidine RNA modifications in bacteria, archaea, and yeast reveals m5C within archaeal mRNAs. PLoS Genet. 2013 Jun;9(6):e1003602. [Content Brief]
[4]. Huang W, et al. Formation and determination of the oxidation products of 5-methylcytosine in RNA. Chem Sci. 2016 Aug 1;7(8):5495-5502. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 7.7429 mL | 38.7147 mL | 77.4293 mL | 193.5734 mL |
| 5 mM | 1.5486 mL | 7.7429 mL | 15.4859 mL | 38.7147 mL | |
| 10 mM | 0.7743 mL | 3.8715 mL | 7.7429 mL | 19.3573 mL | |
| 15 mM | 0.5162 mL | 2.5810 mL | 5.1620 mL | 12.9049 mL | |
| 20 mM | 0.3871 mL | 1.9357 mL | 3.8715 mL | 9.6787 mL | |
| 25 mM | 0.3097 mL | 1.5486 mL | 3.0972 mL | 7.7429 mL | |
| 30 mM | 0.2581 mL | 1.2905 mL | 2.5810 mL | 6.4524 mL | |
| H2O | 40 mM | 0.1936 mL | 0.9679 mL | 1.9357 mL | 4.8393 mL |
| 50 mM | 0.1549 mL | 0.7743 mL | 1.5486 mL | 3.8715 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
5-Methylcytosine-d4 Related Classifications
HY-W008091S Related Classifications
Powered by Bioz