5-Methylcytosine hydrochloride

Cat. No.: HY-W008091A Purity: 99.57%: Data Sheet
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5-Methylcytosine hydrochloride is a well-characterized DNA modification in prokaryotes and eukaryotes. 5-Methylcytosine hydrochloride forms symmetrical methylation on CpG dinucleotides in DNA, stabilizes tRNA/rRNA structure in RNA, and affects mRNA translation. 5-Methylcytosine hydrochloride can be oxidized to generate 5hmC, 5fC, and 5caC. 5-Methylcytosine hydrochloride can be used in epigenetics, developmental biology, and the study of diseases such as colorectal cancer and hepatocellular carcinoma.

For research use only. We do not sell to patients.

5-Methylcytosine hydrochloride Chemical Structure

CAS No. : 58366-64-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote	Estimated Time of Arrival: December 31
5 g	Get quote	Estimated Time of Arrival: December 31
10 g	Get quote	Estimated Time of Arrival: December 31
50 g	Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of 5-Methylcytosine hydrochloride:

5-Methyl cytosine-¹³C,¹⁵N₂ hydrochloride In-stock
5-Methylcytosine In-stock

2 Publications Citing Use of MCE 5-Methylcytosine hydrochloride

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Molecular Weight

161.59

Formula

C₅H₈ClN₃O

CAS No.

58366-64-6

Appearance

Solid

Color

White to off-white

SMILES

O=C1N=CC(C)=C(N)N1.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 5.83 mg/mL (36.08 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 5 mg/mL (30.94 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.1885 mL	30.9425 mL	61.8850 mL
5 mM	1.2377 mL	6.1885 mL	12.3770 mL
10 mM	0.6189 mL	3.0943 mL	6.1885 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.57%

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Data Sheet (274 KB) SDS (393 KB)

COA (250 KB) HNMR (238 KB) LCMS (99 KB)

Handling Instructions (2659 KB)

References

[1]. Cui X, et al. 5-Methylcytosine RNA Methylation in Arabidopsis Thaliana. Mol Plant. 2017 Nov 6;10(11):1387-1399. [Content Brief]

[2]. Breiling A, et al. Epigenetic regulatory functions of DNA modifications: 5-methylcytosine and beyond. Epigenetics Chromatin. 2015 Jul 21;8:24. [Content Brief]

[3]. Edelheit S, et al. Transcriptome-wide mapping of 5-methylcytidine RNA modifications in bacteria, archaea, and yeast reveals m5C within archaeal mRNAs. PLoS Genet. 2013 Jun;9(6):e1003602. [Content Brief]

[4]. Huang W, et al. Formation and determination of the oxidation products of 5-methylcytosine in RNA. Chem Sci. 2016 Aug 1;7(8):5495-5502. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	6.1885 mL	30.9425 mL	61.8850 mL	154.7125 mL
	5 mM	1.2377 mL	6.1885 mL	12.3770 mL	30.9425 mL
	10 mM	0.6189 mL	3.0943 mL	6.1885 mL	15.4713 mL
	15 mM	0.4126 mL	2.0628 mL	4.1257 mL	10.3142 mL
	20 mM	0.3094 mL	1.5471 mL	3.0943 mL	7.7356 mL
	25 mM	0.2475 mL	1.2377 mL	2.4754 mL	6.1885 mL
	30 mM	0.2063 mL	1.0314 mL	2.0628 mL	5.1571 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.