5-Methylcytosine hydrochloride
Based on 2 publication(s) in Google Scholar
5-Methylcytosine hydrochloride is a well-characterized DNA modification in prokaryotes and eukaryotes. 5-Methylcytosine hydrochloride forms symmetrical methylation on CpG dinucleotides in DNA, stabilizes tRNA/rRNA structure in RNA, and affects mRNA translation. 5-Methylcytosine hydrochloride can be oxidized to generate 5hmC, 5fC, and 5caC. 5-Methylcytosine hydrochloride can be used in epigenetics, developmental biology, and the study of diseases such as colorectal cancer and hepatocellular carcinoma.
For research use only. We do not sell to patients.
- Purity: 99.57%
- CAS No.: 58366-64-6
- Formula: C5H8ClN3O
- Molecular Weight:161.59
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-W010450
Thymine
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HY-W010450R
Thymine (Standard)
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HY-B0016
Capecitabine
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HY-W017389
Xanthine
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HY-Y1055
Guanine
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HY-W002272
Isocytosine
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HY-W010128
6-(Dimethylamino)purine
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HY-W017389R
Xanthine (Standard)
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HY-Y1055R
Guanine (Standard)
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HY-B0016R
Capecitabine (Standard)
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HY-50723
3-Methylxanthine
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HY-D0186
2'-Deoxyuridine
Publications Citing Use of MedChemExpress (MCE) 5-Methylcytosine hydrochloride
MoreAll DNA/RNA Synthesis Isoforms
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
Chemical Information
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CAS No. 58366-64-6
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Appearance Solid
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Molecular Weight 161.59
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Formula C5H8ClN3O
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Color White to off-white
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SMILES
O=C1N=CC(C)=C(N)N1.[H]Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (2)
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Journal Impact Factor
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Most Recent
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Nature
2025 Jul;643(8070):192-200. PMID: 39695227 -
J Hazard Mater
DNA hydroxymethylation reprogramming of β-oxidation genes mediates early-life arsenic-evoked hepatic lipid accumulation in adult mice. [Abstract]2022 May 15:430:128511. PMID: 35739688
Solvent & Solubility
DMSO : 5.83 mg/mL (36.08 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 5 mg/mL (30.94 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Cui X, et al. 5-Methylcytosine RNA Methylation in Arabidopsis Thaliana. Mol Plant. 2017 Nov 6;10(11):1387-1399. [Content Brief]
[2]. Breiling A, et al. Epigenetic regulatory functions of DNA modifications: 5-methylcytosine and beyond. Epigenetics Chromatin. 2015 Jul 21;8:24. [Content Brief]
[3]. Edelheit S, et al. Transcriptome-wide mapping of 5-methylcytidine RNA modifications in bacteria, archaea, and yeast reveals m5C within archaeal mRNAs. PLoS Genet. 2013 Jun;9(6):e1003602. [Content Brief]
[4]. Huang W, et al. Formation and determination of the oxidation products of 5-methylcytosine in RNA. Chem Sci. 2016 Aug 1;7(8):5495-5502. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 6.1885 mL | 30.9425 mL | 61.8850 mL | 154.7125 mL |
| 5 mM | 1.2377 mL | 6.1885 mL | 12.3770 mL | 30.9425 mL | |
| 10 mM | 0.6189 mL | 3.0943 mL | 6.1885 mL | 15.4713 mL | |
| 15 mM | 0.4126 mL | 2.0628 mL | 4.1257 mL | 10.3142 mL | |
| 20 mM | 0.3094 mL | 1.5471 mL | 3.0943 mL | 7.7356 mL | |
| 25 mM | 0.2475 mL | 1.2377 mL | 2.4754 mL | 6.1885 mL | |
| 30 mM | 0.2063 mL | 1.0314 mL | 2.0628 mL | 5.1571 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
5-Methylcytosine hydrochloride Related Classifications
HY-W008091A Related Classifications
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