2. GPCR/G Protein
  3. Adenosine Receptor
  4. 9-Ethyladenine

9-Ethyladenine is a precursor of competitive antagonists of adenosine receptors (A1, A2, A3), with no significant inhibitory effect on adenine phosphoribosyltransferase (APRT). 9-Ethyladenine derivatives have high affinity and selectivity for A1 (Ki=27 nM), A2A (Ki=46 nM), and A3 (Ki=86 nM) receptors. 9-Ethyladenine does not inhibit brain APRT activity, can be used in the study of adenosine receptor-related diseases (such as nervous system diseases) models.

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9-Ethyladenine

9-Ethyladenine Estructura química

No. CAS : 2715-68-6

Tamaño Precio Stock Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
100 mg En stock
250 mg En stock
1 g En stock
5 g En stock
10 g   Obtener un presupuesto  
50 g   Obtener un presupuesto  

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Other Forms of 9-Ethyladenine:

9-Ethyladenine Related Antibodies

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Adenosine A1 receptor (A1R) Adenosine A2A receptor (A2AR) Adenosine A2B receptor (A2BR) Adenosine A3 receptor (A3R)

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Descripciòn

9-Ethyladenine is a precursor of competitive antagonists of adenosine receptors (A1, A2, A3), with no significant inhibitory effect on adenine phosphoribosyltransferase (APRT). 9-Ethyladenine derivatives have high affinity and selectivity for A1 (Ki=27 nM), A2A (Ki=46 nM), and A3 (Ki=86 nM) receptors. 9-Ethyladenine does not inhibit brain APRT activity, can be used in the study of adenosine receptor-related diseases (such as nervous system diseases) models[1][2].

IC50 & Target

Microbial Metabolite

 

In Vivo

9-Ethyladenine (2.5×10-6 mol; intraperitoneal injection; 3 times a week; 5 weeks) does not induce self-injurious behavior (such as tissue damage or hair loss) in C57BL/6 wild-type mice and HPRT-deficient mice, without significant inhibition of brain APRT activity[1].
9-Ethyladenine (1.25×10-5 mol; intraperitoneal injection; 3 times a week; 4 weeks) may cause illness or even death in the above mouse models due to chronic toxicity, without self-injurious behavior[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 wild-type male mice (8 weeks old), HPRT-deficient male mice (8 weeks old)[1]
Dosage: 2.5×10-6 mol, 1.25×10-5 mol
Administration: Intraperitoneal injection; 3 times per week; 5 weeks (2.5×10-6 mol) or 4 weeks (1.25×10-5 mol)
Result: Didn't result in self-injurious behavior (SIB), visible injury, or hair loss.
Resulted mice became too sick to move by week 4 at 1.25×10-5 mol dose.
Showed no significant reduction of APRT activity, compared to saline controls in both dosages.
Peso molecular

163.18

Fòrmula

C7H9N5
No. CAS
Appearance

Solid

Color

White to off-white

SMILES

NC1=C2C(N(C=N2)CC)=NC=N1
Structure Classification
Initial Source

Escherichia coli (strain K12, MG1655)
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvente y solubilidad
In Vitro: 

H2O : 100 mg/mL (612.82 mM; Need ultrasonic)

DMSO : 62.5 mg/mL (383.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.1282 mL 30.6410 mL 61.2820 mL
5 mM 1.2256 mL 6.1282 mL 12.2564 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 3 mg/mL (18.38 mM); Clear solution

    This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 3 mg/mL (18.38 mM); Clear solution

    This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 33.33 mg/mL (204.25 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureza y Documentación

Purity: 99.87%

SDS (393 KB)

COA (254 KB) HNMR (136 KB) RP-HPLC (221 KB)

Instrucciones de manejo (2659 KB)
Referencias

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 6.1282 mL 30.6410 mL 61.2820 mL 153.2051 mL
5 mM 1.2256 mL 6.1282 mL 12.2564 mL 30.6410 mL
10 mM 0.6128 mL 3.0641 mL 6.1282 mL 15.3205 mL
15 mM 0.4085 mL 2.0427 mL 4.0855 mL 10.2137 mL
20 mM 0.3064 mL 1.5321 mL 3.0641 mL 7.6603 mL
25 mM 0.2451 mL 1.2256 mL 2.4513 mL 6.1282 mL
30 mM 0.2043 mL 1.0214 mL 2.0427 mL 5.1068 mL
40 mM 0.1532 mL 0.7660 mL 1.5321 mL 3.8301 mL
50 mM 0.1226 mL 0.6128 mL 1.2256 mL 3.0641 mL
60 mM 0.1021 mL 0.5107 mL 1.0214 mL 2.5534 mL
80 mM 0.0766 mL 0.3830 mL 0.7660 mL 1.9151 mL
100 mM 0.0613 mL 0.3064 mL 0.6128 mL 1.5321 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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9-Ethyladenine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

9-Ethyladenine2715-68-6Adenosine ReceptorP1 receptorInhibitorinhibitorinhibit

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Nombre del producto:
9-Ethyladenine
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