Bucladesine sodium (Synonyms: Dibutyryl cAMP sodium; DBcAMP sodium)

Name: Bucladesine sodium
Brand: MedChemExpress
SKU: HY-B0764
Rating: 5.0 (41 reviews)

Cat. No.: HY-B0764 Purity: 99.36%: Data Sheet
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Bucladesine (Dibutyryl cAMP; DBcAMP) sodium is a membrane-permeable 3′, 5′-cyclic adenosine monophosphate (cAMP) analog. Bucladesine selectively activates cAMP dependent protein kinase (PKA) by increasing the intracellular level of cAMP. Bucladesine significantly attenuates MDMA-induced increases in hippocampal mitochondrial ROS formation, mitochondrial outer membrane damage, cytochrome c release, and hippocampal ADP/ATP ratio, thereby improving spatial learning and memory impairments. Bucladesine exhibit anti-nociceptive and anti-inflammation effect. Bucladesine can inhibit cancer cells proliferation, induce apoptosis. Bucladesine can be used for the researches of neurological disease, cancer, inflammation.

For research use only. We do not sell to patients.

CAS No. : 16980-89-5

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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5 g	In-stock	Estimated Time of Arrival: December 31
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Based on 41 publication(s) in Google Scholar

Other Forms of Bucladesine sodium:

41 Publications Citing Use of MCE Bucladesine sodium

Cell Proliferation/Viability Assay

: Bucladesine sodium purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2024 Aug 19;20(11):4476-4495. [Abstract]; The protein expression of p-AMPK (S173), p-AMPK (T172) and AMPK of HT-29 cells treated with 5-HT, either alone or in combination with H-89 (1 μM) and dbcAMP (1 μM).

: Bucladesine sodium purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2022 Sep 15:243:113982. [Abstract]; H295R cells were treated with 30 μM BHPF in the absence or presence of 10 μM forskolin (FSK) or 100 μM dbcAMP for 48 h and evaluated by Western blotting.

: Bucladesine sodium purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2022 Oct 15:932:175231. [Abstract]; B16F10 cells was pretreated or not with 3 μM Bucladesine sodium for 1 h before pterostilbene was applied for 24 h, 48h or 72 h at 3 μM. The CREB and p-CREB protein expression levels were detected by WB.

: Bucladesine sodium purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2022 Oct 15:932:175231. [Abstract]; Cell viability of H295R cells exposed to 0–40 μM BHPF in the presence of 100 μM dbcAMP for 48 h.

: Bucladesine sodium purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2022 Oct 15:932:175231. [Abstract]; Micrographs presenting the incorporation of EdU in H295R cells exposed to BHPF or combined with 100 μM dbcAMP for 48 h. Red: the EdU positive cells; blue: the cell nuclei (Hoechst).

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Biological Activity
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Description

IC₅₀ & Target^[5]

PKA

PDE

In Vitro

Bucladesine causes significant growth delay in multicellular tumor spheroids derived directly from human malignant brain tumors^[1].
Bucladesine augments differentiation therapy (in combination with Sodium butyrate (HY-B0350A) and hyperthermia) in human and canine brain tumor cells in vitro^[1].
Bucladesine suppresses the mitogenic effects of platelet-derived growth factor (PDGF), basic fibroblast growth factor (bFGF), and epidermal growth factor (EGF) on human astrocytoma cells; bucladesine alone neither potentiates nor inhibits human astrocytoma cell growth^[1].
Bucladesine suppresses proliferation, enhances cytoplasmic process formation, increases cytoplasmic glial fibrillary acidic protein (GFAP) levels, and suppresses malignant glioma invasion by decreasing CD44 isoform expression in cultured malignant glioma cells, inducing biochemical and morphological changes^[1].
Bucladesine decreases Bcl-2 levels and enhances SNAP-induced p53-sensitive cell death and MPP⁺-induced cell death, sensitizing human neuroblastoma cells to apoptosis^[1].
Bucladesine inhibits proliferation and induces morphological differentiation of astrocytes in vitro via a cyclic adenosine monophosphate-dependent protein kinase pathway^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Bucladesine (100 μM; bilateral intrahippocampal infusion; once daily; 4 consecutive days) significantly attenuates 3,4-methylenedioxymethamphetamine (MDMA)-induced spatial learning and memory impairments and hippocampal mitochondrial dysfunction in adult male Wistar rats^[2].
Bucladesine (50-300 nM/mouse; i.p.; single dose) exerts a biphasic anti-nociceptive effect on thermal-induced acute pain in male albino mice, with significant activity at 50 and 100 nM/mouse doses, no significant effect at 300 nM/mouse^[3].
Bucladesine (0.5-1.5% cream, 5% solution; topical; single or two doses) produces a statistically significant reduction in Arachidonic acid (HY-109590)-induced acute skin inflammation in Mus musculus, with single administration of 1.5% cream yielding a 28% reduction in ear swelling and two administrations yielding a 24% reduction^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

491.37

Formula

C₁₈H₂₃N₅NaO₈P

CAS No.

16980-89-5

Appearance

Solid

Color

White to off-white

SMILES

O=C(CCC)O[C@H]1[C@H](N2C(N=CN=C3NC(CCC)=O)=C3N=C2)O[C@@](CO4)([H])[C@@]1([H])OP4(O[Na])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (203.51 mM)

DMSO : 100 mg/mL (203.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0351 mL	10.1756 mL	20.3513 mL
5 mM	0.4070 mL	2.0351 mL	4.0703 mL
10 mM	0.2035 mL	1.0176 mL	2.0351 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.36%

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Data Sheet (279 KB) SDS (397 KB)

COA (250 KB) HNMR (459 KB) RP-HPLC (256 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Dalbasti T, et al. Local interstitial chemotherapy with sustained release bucladesine in de novo glioblastoma multiforme: a preliminary study. J Neurooncol. 2002;56(2):167-174.

[2]. Dalbasti T, et al. Local interstitial chemotherapy with sustained release bucladesine in de novo glioblastoma multiforme: a preliminary study. J Neurooncol. 2002;56(2):167-174.

[3]. Salehi F, et al. Effect of bucladesine, pentoxifylline, and H-89 as cyclic adenosine monophosphate analog, phosphodiesterase, and protein kinase A inhibitor on acute pain. Fundam Clin Pharmacol. 2017;31(4):411-419. [Content Brief]

[4]. Rundfeldt C, et al. The stable cyclic adenosine monophosphate analogue, dibutyryl cyclo-adenosine monophosphate (bucladesine), is active in a model of acute skin inflammation. Arch Dermatol Res. 2012;304(4):313-317. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.0351 mL	10.1756 mL	20.3513 mL	50.8782 mL
	5 mM	0.4070 mL	2.0351 mL	4.0703 mL	10.1756 mL
	10 mM	0.2035 mL	1.0176 mL	2.0351 mL	5.0878 mL
	15 mM	0.1357 mL	0.6784 mL	1.3568 mL	3.3919 mL
	20 mM	0.1018 mL	0.5088 mL	1.0176 mL	2.5439 mL
	25 mM	0.0814 mL	0.4070 mL	0.8141 mL	2.0351 mL
	30 mM	0.0678 mL	0.3392 mL	0.6784 mL	1.6959 mL
	40 mM	0.0509 mL	0.2544 mL	0.5088 mL	1.2720 mL
	50 mM	0.0407 mL	0.2035 mL	0.4070 mL	1.0176 mL
	60 mM	0.0339 mL	0.1696 mL	0.3392 mL	0.8480 mL
	80 mM	0.0254 mL	0.1272 mL	0.2544 mL	0.6360 mL
	100 mM	0.0204 mL	0.1018 mL	0.2035 mL	0.5088 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Bucladesine sodium Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Bucladesine sodium (Synonyms: Dibutyryl cAMP sodium; DBcAMP sodium)

Customer Review

Other Forms of Bucladesine sodium:

Bucladesine sodium Related Antibodies

41 Publications Citing Use of MCE Bucladesine sodium

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All PKA Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Bucladesine sodium Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Bucladesine sodium Related Classifications

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