Chlorambucil (Synonyms: CB-1348; WR-139013)

Name: Chlorambucil
Brand: MedChemExpress
SKU: HY-13593
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Cat. No.: HY-13593 Pureté: 99.21%: Fiche technique
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Chlorambucil (CB-1348), an orally active antineoplastic agent, is a CNS-penetrant bifunctional alkylating agent belonging to the nitrogen mustard group. Chlorambucil can be used for the research of lymphocytic leukemia, ovarian and breast carcinomas, and Hodgkin’s disease.

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Chlorambucil Chemical Structure

CAS No. : 305-03-3

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Échantillon gratuit (0.1 - 0.2 mg)	Appliquer maintenant
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
100 mg	En stock	Estimated Time of Arrival: December 31
500 mg	En stock	Estimated Time of Arrival: December 31
1 g	En stock	Estimated Time of Arrival: December 31
5 g	En stock	Estimated Time of Arrival: December 31
10 g	Obtenir un devis
50 g	Obtenir un devis

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Based on 4 publication(s) in Google Scholar

Other Forms of Chlorambucil:

Chlorambucil-d₈ Obtenir un devis
Chlorambucil-d₈-1 Obtenir un devis
Chlorambucil (Standard) Obtenir un devis

4 Publications Citing Use of MCE Chlorambucil

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•Related Small Molecules:

•Related Proteins:

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Description

IC₅₀ & Target

DNA Alkylator^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
4T1	IC₅₀	>100 3 Compound: Chlorambucil	Antiproliferative activity against mouse 4T1 cells after 72 hrs by MTT assay Antiproliferative activity against mouse 4T1 cells after 72 hrs by MTT assay	[PMID: 28129978]
2008	IC₅₀	12500 1 Compound: Chl	Cytotoxicity against human 2008 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human 2008 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 30996795]
5637	IC₅₀	6.55 3 Compound: Chlorambucil	Antiproliferative activity against human 5637 cells after 96 hrs by crystal violet assay Antiproliferative activity against human 5637 cells after 96 hrs by crystal violet assay	[PMID: 18434163]
4T1	IC₅₀	> 100 3 Compound: Chlorambucil	Antiproliferative activity against mouse 4T1 cells after 72 hrs by MTT assay Antiproliferative activity against mouse 4T1 cells after 72 hrs by MTT assay	[PMID: 28129978]
5637	IC₅₀	6.55 3 Compound: Chlo	Cytotoxicity against human 5637 cells assessed as growth inhibition after 96 hrs by crystal violet staining assay Cytotoxicity against human 5637 cells assessed as growth inhibition after 96 hrs by crystal violet staining assay	10.1039/C3MD00252G
5637	IC₅₀	6.55 3 Compound: Chlo	Cytotoxicity against human 5637 cells assessed as growth inhibition after 96 hrs by crystal violet staining assay Cytotoxicity against human 5637 cells assessed as growth inhibition after 96 hrs by crystal violet staining assay	10.1039/C3MD00252G
5637	IC₅₀	6.55 3 Compound: Chlorambucil	Antiproliferative activity against human 5637 cells after 96 hrs by crystal violet assay Antiproliferative activity against human 5637 cells after 96 hrs by crystal violet assay	[PMID: 18434163]
786-0	IC₅₀	55.5 3 Compound: Chlorambucil	Anticancer activity against human 786-0 cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay Anticancer activity against human 786-0 cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay	[PMID: 28388522]
786-0	IC₅₀	55.5 3 Compound: Chlorambucil	Anticancer activity against human 786-0 cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay Anticancer activity against human 786-0 cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay	[PMID: 28388522]
A 172	GI₅₀	>25 3 Compound: CLB	Growth inhibition of human A172 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human A172 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
A 172	GI₅₀	> 25 3 Compound: CLB	Growth inhibition of human A172 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human A172 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
A2780	IC₅₀	12 3 Compound: CLB	Antiproliferative activity against human A2780 cells after 72 hrs at 37 degC by MTT assay Antiproliferative activity against human A2780 cells after 72 hrs at 37 degC by MTT assay	10.1039/C6MD00271D
A2780	IC₅₀	52.5 3 Compound: chlorambucil	Growth inhibition against A2780 cisplatin resistant wild-type ovarian cell lines Growth inhibition against A2780 cisplatin resistant wild-type ovarian cell lines	[PMID: 15027879]
A 172	GI₅₀	> 25 3 Compound: CLB	Growth inhibition of human A172 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human A172 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
A2780cis	IC₅₀	43 3 Compound: CLB	Antiproliferative activity against human A2780cis cells after 72 hrs at 37 degC by MTT assay Antiproliferative activity against human A2780cis cells after 72 hrs at 37 degC by MTT assay	10.1039/C6MD00271D
A-375	CC₅₀	127 3 Compound: Chlorambucil	Cytotoxicity against human A375 cells after 24 hrs by MTT assay Cytotoxicity against human A375 cells after 24 hrs by MTT assay	[PMID: 20615582]
A-375	IC₅₀	69.2 3 Compound: Chlorambucil	Antiproliferative activity against human A375 cells after 72 hrs by CCK8 assay Antiproliferative activity against human A375 cells after 72 hrs by CCK8 assay	[PMID: 28818449]
A-375	IC₅₀	69.2 3 Compound: Chlorambucil	Antiproliferative activity against human A375 cells after 72 hrs CCK8 assay Antiproliferative activity against human A375 cells after 72 hrs CCK8 assay	[PMID: 30108928]
A-375	IC₅₀	2.58 3 Compound: C5	Cytotoxicity against human A375 cells after 5 days by MTT assay Cytotoxicity against human A375 cells after 5 days by MTT assay	[PMID: 19121874]
A-427	IC₅₀	9.5 3 Compound: Chlorambucil	Antiproliferative activity against human A427 cells after 96 hrs by crystal violet assay Antiproliferative activity against human A427 cells after 96 hrs by crystal violet assay	[PMID: 18434163]
A498	IC₅₀	280 3 Compound: Chlorambucil	Anticancer activity against human A498 cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay Anticancer activity against human A498 cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay	[PMID: 28388522]
A549	GI₅₀	6.1 3 Compound: 1	Growth inhibition of human A549 cells expressing p53 after 7 days by celltiter-glo assay Growth inhibition of human A549 cells expressing p53 after 7 days by celltiter-glo assay	[PMID: 20973561]
A549	IC₅₀	22.2 3 Compound: Chlorambucil	Antiproliferative activity against human A549 cells after 72 hrs by CCK8 assay Antiproliferative activity against human A549 cells after 72 hrs by CCK8 assay	[PMID: 28818449]
A549	IC₅₀	>50 3 Compound: Chlorambucil	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 30243153]
A549	IC₅₀	22.2 3 Compound: Chlorambucil	Antiproliferative activity against human A549 cells after 72 hrs CCK8 assay Antiproliferative activity against human A549 cells after 72 hrs CCK8 assay	[PMID: 30108928]
A549	IC₅₀	>40 3 Compound: CLB; 3d	Antiproliferative activity against human A549 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36240546]
A549	IC₅₀	72.78 3 Compound: Chlorambucil	Antiproliferative activity against human A549 cells incubated for 72 hrs by SRB assay Antiproliferative activity against human A549 cells incubated for 72 hrs by SRB assay	[PMID: 31082763]
A549	IC₅₀	43 3 Compound: CLB	Cytotoxicity against human A549 assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test Cytotoxicity against human A549 assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test	[PMID: 28927903]
A549	IC₅₀	21.09 6 Compound: Chlorambucil	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	10.1007/s00044-013-0582-8
A549	IC₅₀	18.8 3 Compound: Chlorambucil	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 3 days by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 3 days by MTT assay	[PMID: 29649739]
A549	IC₅₀	43 3 Compound: 2, Chlorambucil, CLB	Cytotoxicity against human A549 cells by resazurin reduction test Cytotoxicity against human A549 cells by resazurin reduction test	[PMID: 18424156]
A549	IC₅₀	24.47 3 Compound: Chlorambucil	Growth inhibition of human A549 cells incubated for 5 days by SRB assay Growth inhibition of human A549 cells incubated for 5 days by SRB assay	[PMID: 32145644]
ACHN	IC₅₀	133 3 Compound: Chlorambucil	Anticancer activity against human ACHN cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay Anticancer activity against human ACHN cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay	[PMID: 28388522]
B16	IC₅₀	>1 3 Compound: 2; CLB	Antitumor activity against mouse B16 cells assessed as inhibition of cell viability incubated for 72 hrs by SRB assay Antitumor activity against mouse B16 cells assessed as inhibition of cell viability incubated for 72 hrs by SRB assay	[PMID: 38060985]
B16	IC₅₀	5.1 3 Compound: 1	In vitro cytotoxicity activity against B16 cells, by colony forming assay In vitro cytotoxicity activity against B16 cells, by colony forming assay	[PMID: 11985479]
B16	IC₅₀	279.8 3 Compound: chlorambucil	The compound was tested for inhibition of B16-BL6 melanoma cells growing in culture with 0.25 mM DFMO. The compound was tested for inhibition of B16-BL6 melanoma cells growing in culture with 0.25 mM DFMO.	[PMID: 1447731]
B16	IC₅₀	220.3 3 Compound: chlorambucil	The compound was tested for inhibition of B16-BL6 melanoma cells growing in culture. The compound was tested for inhibition of B16-BL6 melanoma cells growing in culture.	[PMID: 1447731]
B16-F10	IC₅₀	12.59 3 Compound: Chlorambucil	Antiproliferative activity against mouse B16F10 cells incubated for 72 hrs by MTT assay Antiproliferative activity against mouse B16F10 cells incubated for 72 hrs by MTT assay	[PMID: 31082763]
BALB/3T3	IC₅₀	69.72 3 Compound: Chlorambucil	Antiproliferative activity against mouse BALB/3T3 cells incubated for 72 hrs by SRB assay Antiproliferative activity against mouse BALB/3T3 cells incubated for 72 hrs by SRB assay	[PMID: 31082763]
Bel-7402	IC₅₀	49.31 3 Compound: chlorambucil	Anticancer activity against human Bel7402 cells assessed as growth inhibition by MTT assay Anticancer activity against human Bel7402 cells assessed as growth inhibition by MTT assay	[PMID: 25050168]
Bel-7402	IC₅₀	>100 3 Compound: 12	Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay	[PMID: 29407983]
Bel-7402	IC₅₀	>50 3 Compound: Chlorambucil	Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay	[PMID: 30243153]
A-375	CC₅₀	127 3 Compound: Chlorambucil	Cytotoxicity against human A375 cells after 24 hrs by MTT assay Cytotoxicity against human A375 cells after 24 hrs by MTT assay	[PMID: 20615582]
Bel-7402	IC₅₀	>80 3 Compound: Chlorambucil	Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay	[PMID: 29524728]
Bel7402/5-FU	IC₅₀	>100 3 Compound: 12	Antiproliferative activity against human Bel7402/5-FU cells after 72 hrs by MTT assay Antiproliferative activity against human Bel7402/5-FU cells after 72 hrs by MTT assay	[PMID: 29407983]
A-375	IC₅₀	2.58 3 Compound: C5	Cytotoxicity against human A375 cells after 5 days by MTT assay Cytotoxicity against human A375 cells after 5 days by MTT assay	[PMID: 19121874]
Bel7402/5-FU	IC₅₀	>80 3 Compound: Chlorambucil	Antiproliferative activity against human Bel7402/5-FU cells after 72 hrs by MTT assay Antiproliferative activity against human Bel7402/5-FU cells after 72 hrs by MTT assay	[PMID: 29524728]
A-375	IC₅₀	69.2 3 Compound: Chlorambucil	Antiproliferative activity against human A375 cells after 72 hrs by CCK8 assay Antiproliferative activity against human A375 cells after 72 hrs by CCK8 assay	[PMID: 28818449]
A-375	IC₅₀	69.2 3 Compound: Chlorambucil	Antiproliferative activity against human A375 cells after 72 hrs CCK8 assay Antiproliferative activity against human A375 cells after 72 hrs CCK8 assay	[PMID: 30108928]
BT-474	CC₅₀	>150 3 Compound: Chlorambucil	Cytotoxicity against human BT474 cells assessed as decrease in cell viability after 48 hrs by MTS assay Cytotoxicity against human BT474 cells assessed as decrease in cell viability after 48 hrs by MTS assay	[PMID: 29043812]
A-427	IC₅₀	9.5 3 Compound: Chlorambucil	Antiproliferative activity against human A427 cells after 96 hrs by crystal violet assay Antiproliferative activity against human A427 cells after 96 hrs by crystal violet assay	[PMID: 18434163]
BT-474	CC₅₀	>150 3 Compound: Chlorambucil	Cytotoxicity against human BT-474 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Cytotoxicity against human BT-474 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 29126728]
BT-474	IC₅₀	237.5 3 Compound: 1	Cytotoxicity against human BT474 cells after 72 hrs by MTT assay Cytotoxicity against human BT474 cells after 72 hrs by MTT assay	[PMID: 24147900]
A2780	IC₅₀	12 3 Compound: CLB	Antiproliferative activity against human A2780 cells after 72 hrs at 37 degC by MTT assay Antiproliferative activity against human A2780 cells after 72 hrs at 37 degC by MTT assay	10.1039/C6MD00271D
A2780	IC₅₀	52.5 3 Compound: chlorambucil	Growth inhibition against A2780 cisplatin resistant wild-type ovarian cell lines Growth inhibition against A2780 cisplatin resistant wild-type ovarian cell lines	[PMID: 15027879]
BT-549	IC₅₀	>200 3 Compound: 1	Cytotoxicity against human BT549 cells after 72 hrs by MTT assay Cytotoxicity against human BT549 cells after 72 hrs by MTT assay	[PMID: 24147900]
A2780cis	IC₅₀	43 3 Compound: CLB	Antiproliferative activity against human A2780cis cells after 72 hrs at 37 degC by MTT assay Antiproliferative activity against human A2780cis cells after 72 hrs at 37 degC by MTT assay	10.1039/C6MD00271D
BV-173	IC₅₀	34 3 Compound: Chlorambucil	In vitro antiproliferative activity against human BV173 cells assessed as decrease in cell viability after 24 hrs by MTT assay In vitro antiproliferative activity against human BV173 cells assessed as decrease in cell viability after 24 hrs by MTT assay	[PMID: 24681986]
A498	IC₅₀	280 3 Compound: Chlorambucil	Anticancer activity against human A498 cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay Anticancer activity against human A498 cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay	[PMID: 28388522]
BXPC-3	IC₅₀	>100 3 Compound: 1	Cytotoxicity against human BxPC3 cells after 72 hrs by MTT assay Cytotoxicity against human BxPC3 cells after 72 hrs by MTT assay	[PMID: 24147900]
CAMA-1	IC₅₀	191.7 3 Compound: 1	Cytotoxicity against human CAMA1 cells after 72 hrs by MTT assay Cytotoxicity against human CAMA1 cells after 72 hrs by MTT assay	[PMID: 24147900]
A549	GI₅₀	6.1 3 Compound: 1	Growth inhibition of human A549 cells expressing p53 after 7 days by celltiter-glo assay Growth inhibition of human A549 cells expressing p53 after 7 days by celltiter-glo assay	[PMID: 20973561]
A549	IC₅₀	18.8 3 Compound: Chlorambucil	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 3 days by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 3 days by MTT assay	[PMID: 29649739]
CCRF-CEM	IC₅₀	12.025 3 Compound: CLB; 3d	Antiproliferative activity against human CCRF-CEM cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human CCRF-CEM cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36240546]
A549	IC₅₀	21.09 6 Compound: Chlorambucil	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	10.1007/s00044-013-0582-8
CCRF-CEM	IC₅₀	21 3 Compound: Chlorambucil	In vitro antiproliferative activity against human CCRF-CEM cells assessed as decrease in cell viability after 24 hrs by MTT assay In vitro antiproliferative activity against human CCRF-CEM cells assessed as decrease in cell viability after 24 hrs by MTT assay	[PMID: 24681986]
A549	IC₅₀	22.2 3 Compound: Chlorambucil	Antiproliferative activity against human A549 cells after 72 hrs by CCK8 assay Antiproliferative activity against human A549 cells after 72 hrs by CCK8 assay	[PMID: 28818449]
CH1	IC₅₀	7.9 3 Compound: chlorambucil	Growth inhibition against CH-1 cisplatin resistant wild-type ovarian cell lines Growth inhibition against CH-1 cisplatin resistant wild-type ovarian cell lines	[PMID: 15027879]
A549	IC₅₀	22.2 3 Compound: Chlorambucil	Antiproliferative activity against human A549 cells after 72 hrs CCK8 assay Antiproliferative activity against human A549 cells after 72 hrs CCK8 assay	[PMID: 30108928]
CH1	IC₅₀	3.3 3 Compound: chlorambucil	Growth inhibition against CH-1 wild-type ovarian cell lines Growth inhibition against CH-1 wild-type ovarian cell lines	[PMID: 15027879]
A549	IC₅₀	24.47 3 Compound: Chlorambucil	Growth inhibition of human A549 cells incubated for 5 days by SRB assay Growth inhibition of human A549 cells incubated for 5 days by SRB assay	[PMID: 32145644]
CHO	IC₅₀	84.5 3 Compound: CA, chlorambucil	Cytotoxicity against PAT active CHO cells after 48 hrs Cytotoxicity against PAT active CHO cells after 48 hrs	[PMID: 18281932]
A549	IC₅₀	43 3 Compound: 2, Chlorambucil, CLB	Cytotoxicity against human A549 cells by resazurin reduction test Cytotoxicity against human A549 cells by resazurin reduction test	[PMID: 18424156]
CHO	IC₅₀	30 3 Compound: Chlorambucil	Dose required to inhibit cell growth was determined against CHO cell line Dose required to inhibit cell growth was determined against CHO cell line	[PMID: 11262080]
A549	IC₅₀	43 3 Compound: CLB	Cytotoxicity against human A549 assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test Cytotoxicity against human A549 assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test	[PMID: 28927903]
CHO-AA8	IC₅₀	26 3 Compound: cholambucil	Compound was evaluated for in vitro cytotoxicities against Chinese hamster ovary derived AA8 cell lines. Compound was evaluated for in vitro cytotoxicities against Chinese hamster ovary derived AA8 cell lines.	[PMID: 1992124]
A549	IC₅₀	72.78 3 Compound: Chlorambucil	Antiproliferative activity against human A549 cells incubated for 72 hrs by SRB assay Antiproliferative activity against human A549 cells incubated for 72 hrs by SRB assay	[PMID: 31082763]
CHO-AA8	IC₅₀	26 3 Compound: Chlorambucil	Cytotoxicity against AA8 cell line Cytotoxicity against AA8 cell line	[PMID: 2231599]
CHO-AA8	IC₅₀	38 3 Compound: 3	Growth inhibition activity was measured in repair proficient AA8 cells under aerobic conditions (4 hours drug exposure) Growth inhibition activity was measured in repair proficient AA8 cells under aerobic conditions (4 hours drug exposure)	[PMID: 7707327]
A549	IC₅₀	85.7 1 Compound: Chlorambucil	Cytotoxicity against human A549 cells assessed as cell viability after 5 days by cell viability analyzer Cytotoxicity against human A549 cells assessed as cell viability after 5 days by cell viability analyzer	[PMID: 26291039]
A549	IC₅₀	> 40 3 Compound: CLB; 3d	Antiproliferative activity against human A549 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36240546]
CHO-AA8	IC₅₀	26 3 Compound: Chlorambucil	Inhibition of cell growth in culture against Chinese hamster ovary derived cell line, AA8 Inhibition of cell growth in culture against Chinese hamster ovary derived cell line, AA8	[PMID: 2033580]
A549	IC₅₀	> 50 3 Compound: Chlorambucil	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 30243153]
CHO-AA8	IC₅₀	26 3 Compound: 2 (chlorambucil)	Tested for the in vitro cytotoxicity against transformed chinese hamster fibroblast line AA8 Tested for the in vitro cytotoxicity against transformed chinese hamster fibroblast line AA8	[PMID: 2319563]
CHO-MG	IC₅₀	111.2 3 Compound: CA, chlorambucil	Cytotoxicity against PAT deficient CHO-MG cells after 48 hrs Cytotoxicity against PAT deficient CHO-MG cells after 48 hrs	[PMID: 18281932]
ACHN	IC₅₀	133 3 Compound: Chlorambucil	Anticancer activity against human ACHN cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay Anticancer activity against human ACHN cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay	[PMID: 28388522]
COLO 205	IC₅₀	26.67 6 Compound: Chlorambucil	Cytotoxicity against human COLO205 cells after 72 hrs by MTT assay Cytotoxicity against human COLO205 cells after 72 hrs by MTT assay	10.1007/s00044-013-0582-8
B16	IC₅₀	220.3 3 Compound: chlorambucil	The compound was tested for inhibition of B16-BL6 melanoma cells growing in culture. The compound was tested for inhibition of B16-BL6 melanoma cells growing in culture.	[PMID: 1447731]
COLO 205	IC₅₀	18.8 3 Compound: Chlorambucil	Cytotoxicity against human COLO205 cells assessed as reduction in cell viability after 3 days by MTT assay Cytotoxicity against human COLO205 cells assessed as reduction in cell viability after 3 days by MTT assay	[PMID: 29649739]
COS-7	IC₅₀	43.51 3 Compound: Chlorambucil	Cytotoxicity against African green monkey COS7 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Cytotoxicity against African green monkey COS7 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 32527536]
B16	IC₅₀	279.8 3 Compound: chlorambucil	The compound was tested for inhibition of B16-BL6 melanoma cells growing in culture with 0.25 mM DFMO. The compound was tested for inhibition of B16-BL6 melanoma cells growing in culture with 0.25 mM DFMO.	[PMID: 1447731]
B16	IC₅₀	5.1 3 Compound: 1	In vitro cytotoxicity activity against B16 cells, by colony forming assay In vitro cytotoxicity activity against B16 cells, by colony forming assay	[PMID: 11985479]
CT26	IC₅₀	>1 3 Compound: 2; CLB	Antitumor activity against mouse CT26 cells assessed as inhibition of cell viability incubated for 72 hrs by SRB assay Antitumor activity against mouse CT26 cells assessed as inhibition of cell viability incubated for 72 hrs by SRB assay	[PMID: 38060985]
DAN-G	IC₅₀	27.8 3 Compound: Chlo	Cytotoxicity against human DAN-G cells assessed as growth inhibition after 96 hrs by crystal violet staining assay Cytotoxicity against human DAN-G cells assessed as growth inhibition after 96 hrs by crystal violet staining assay	10.1039/C3MD00252G
B16	IC₅₀	> 1000 1 Compound: 2; CLB	Antitumor activity against mouse B16 cells assessed as inhibition of cell viability incubated for 72 hrs by SRB assay Antitumor activity against mouse B16 cells assessed as inhibition of cell viability incubated for 72 hrs by SRB assay	[PMID: 38060985]
Daoy	IC₅₀	1.36 3 Compound: C5	Cytotoxicity against human DaOY cells after 5 days by MTT assay Cytotoxicity against human DaOY cells after 5 days by MTT assay	[PMID: 19121874]
DLD-1	IC₅₀	47 3 Compound: 2, Chlorambucil, CLB	Cytotoxicity against human DLD1 cells by resazurin reduction test Cytotoxicity against human DLD1 cells by resazurin reduction test	[PMID: 18424156]
DU-145	CC₅₀	>150 3 Compound: Chlorambucil	Cytotoxicity against human DU145 cells assessed as decrease in cell viability after 48 hrs by MTS assay Cytotoxicity against human DU145 cells assessed as decrease in cell viability after 48 hrs by MTS assay	[PMID: 29043812]
DU-145	CC₅₀	>150 3 Compound: Chlorambucil	Cytotoxicity against human DU-145 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Cytotoxicity against human DU-145 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 29126728]
DU-145	GI₅₀	9.4 3 Compound: 1	Growth inhibition of human DU145 cells after 7 days by celltiter-glo assay Growth inhibition of human DU145 cells after 7 days by celltiter-glo assay	[PMID: 20973561]
Fibroblast	IC₅₀	99 3 Compound: CLB	Cytotoxicity against human fibroblasts assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test Cytotoxicity against human fibroblasts assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test	[PMID: 28927903]
B16-F10	IC₅₀	12.59 3 Compound: Chlorambucil	Antiproliferative activity against mouse B16F10 cells incubated for 72 hrs by MTT assay Antiproliferative activity against mouse B16F10 cells incubated for 72 hrs by MTT assay	[PMID: 31082763]
BALB/3T3	IC₅₀	69.72 3 Compound: Chlorambucil	Antiproliferative activity against mouse BALB/3T3 cells incubated for 72 hrs by SRB assay Antiproliferative activity against mouse BALB/3T3 cells incubated for 72 hrs by SRB assay	[PMID: 31082763]
Fibroblast	IC₅₀	99 3 Compound: 2, Chlorambucil, CLB	Cytotoxicity against human primary fibroblasts by resazurin reduction test Cytotoxicity against human primary fibroblasts by resazurin reduction test	[PMID: 18424156]
HCT-116	GI₅₀	105 3 Compound: Chlorambucil	Tested for in vitro concentration required to inhibit growth by 50% against HCT116 human colon cancer cell line Tested for in vitro concentration required to inhibit growth by 50% against HCT116 human colon cancer cell line	[PMID: 11755363]
BT-474	CC₅₀	> 150 3 Compound: Chlorambucil	Cytotoxicity against human BT474 cells assessed as decrease in cell viability after 48 hrs by MTS assay Cytotoxicity against human BT474 cells assessed as decrease in cell viability after 48 hrs by MTS assay	[PMID: 29043812]
HCT-116	IC₅₀	57.6 3 Compound: Chlorambucil	Antiproliferative activity against human HCT116 cells after 72 hrs by CCK8 assay Antiproliferative activity against human HCT116 cells after 72 hrs by CCK8 assay	[PMID: 28818449]
BT-474	CC₅₀	> 150 3 Compound: Chlorambucil	Cytotoxicity against human BT-474 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Cytotoxicity against human BT-474 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 29126728]
BT-474	IC₅₀	237.5 3 Compound: 1	Cytotoxicity against human BT474 cells after 72 hrs by MTT assay Cytotoxicity against human BT474 cells after 72 hrs by MTT assay	[PMID: 24147900]
HCT-116	IC₅₀	>40 3 Compound: CLB; 3d	Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36240546]
BT-549	IC₅₀	> 200 3 Compound: 1	Cytotoxicity against human BT549 cells after 72 hrs by MTT assay Cytotoxicity against human BT549 cells after 72 hrs by MTT assay	[PMID: 24147900]
HCT-15	GI₅₀	8.1 3 Compound: 1	Growth inhibition of human HCT15 cells after 7 days by celltiter-glo assay Growth inhibition of human HCT15 cells after 7 days by celltiter-glo assay	[PMID: 20973561]
BV-173	IC₅₀	34 3 Compound: Chlorambucil	In vitro antiproliferative activity against human BV173 cells assessed as decrease in cell viability after 24 hrs by MTT assay In vitro antiproliferative activity against human BV173 cells assessed as decrease in cell viability after 24 hrs by MTT assay	[PMID: 24681986]
HCT-15	IC₅₀	3.11 3 Compound: Chlorambucil	Anticancer activity against human HCT15 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Anticancer activity against human HCT15 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 32527536]
HEK293	IC₅₀	25 3 Compound: CLB	Antiproliferative activity against HEK293 cells after 72 hrs at 37 degC by CellTiter-Glo luminescence assay Antiproliferative activity against HEK293 cells after 72 hrs at 37 degC by CellTiter-Glo luminescence assay	10.1039/C6MD00271D
BXPC-3	IC₅₀	75300 1 Compound: Chl	Cytotoxicity against human BxPC3 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human BxPC3 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 30996795]
Hep 3B2	GI₅₀	5.1 3 Compound: 1	Growth inhibition of human p53 deficient Hep3B cells after 7 days by celltiter-glo assay Growth inhibition of human p53 deficient Hep3B cells after 7 days by celltiter-glo assay	[PMID: 20973561]
BXPC-3	IC₅₀	> 100 3 Compound: 1	Cytotoxicity against human BxPC3 cells after 72 hrs by MTT assay Cytotoxicity against human BxPC3 cells after 72 hrs by MTT assay	[PMID: 24147900]
HepG2	IC₅₀	111.8 3 Compound: Chlorambucil	Antiproliferative activity against human HepG2 cells after 72 hrs by CCK8 assay Antiproliferative activity against human HepG2 cells after 72 hrs by CCK8 assay	[PMID: 28818449]
Bel-7402	IC₅₀	49.31 3 Compound: chlorambucil	Anticancer activity against human Bel7402 cells assessed as growth inhibition by MTT assay Anticancer activity against human Bel7402 cells assessed as growth inhibition by MTT assay	[PMID: 25050168]
HepG2	IC₅₀	111.8 3 Compound: Chlorambucil	Antiproliferative activity against human HepG2 cells after 72 hrs CCK8 assay Antiproliferative activity against human HepG2 cells after 72 hrs CCK8 assay	[PMID: 30108928]
Bel-7402	IC₅₀	49.31 3 Compound: chlorambucil	Antiproliferative activity against human Bel7402 cells assessed as growth inhibition after 72 hrs by MTT assay Antiproliferative activity against human Bel7402 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25050168]
HepG2	IC₅₀	>100 3 Compound: Chlorambucil	Antiproliferative activity against human HepG2 cells treated for 24 hrs measured after 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells treated for 24 hrs measured after 72 hrs by MTT assay	[PMID: 29037951]
Bel-7402	IC₅₀	> 100 3 Compound: 12	Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay	[PMID: 29407983]
Bel-7402	IC₅₀	> 50 3 Compound: Chlorambucil	Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay	[PMID: 30243153]
HepG2	IC₅₀	190 3 Compound: CLB	Cytotoxicity against human HepG2 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 28818459]
Bel-7402	IC₅₀	> 80 3 Compound: Chlorambucil	Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay	[PMID: 29524728]
HepG2/Adm	IC₅₀	297.1 3 Compound: CLB	Cytotoxicity against human HepG2/ADM cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Cytotoxicity against human HepG2/ADM cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 28818459]
HL-60	IC₅₀	11.63 3 Compound: 12	Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay	[PMID: 29407983]
Bel7402/5-FU	IC₅₀	> 100 3 Compound: 12	Antiproliferative activity against human Bel7402/5-FU cells after 72 hrs by MTT assay Antiproliferative activity against human Bel7402/5-FU cells after 72 hrs by MTT assay	[PMID: 29407983]
Bel7402/5-FU	IC₅₀	> 80 3 Compound: Chlorambucil	Antiproliferative activity against human Bel7402/5-FU cells after 72 hrs by MTT assay Antiproliferative activity against human Bel7402/5-FU cells after 72 hrs by MTT assay	[PMID: 29524728]
HL-60	IC₅₀	11.63 3 Compound: Chlorambucil	Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay	[PMID: 29524728]
CAMA-1	IC₅₀	191.7 3 Compound: 1	Cytotoxicity against human CAMA1 cells after 72 hrs by MTT assay Cytotoxicity against human CAMA1 cells after 72 hrs by MTT assay	[PMID: 24147900]
HL-60	IC₅₀	34.392 3 Compound: CLB; 3d	Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36240546]
CCRF-CEM	IC₅₀	12.025 3 Compound: CLB; 3d	Antiproliferative activity against human CCRF-CEM cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human CCRF-CEM cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36240546]
HL-60	IC₅₀	12.87 3 Compound: Chlorambucil	Antiproliferative activity against human HL60 cells treated for 24 hrs measured after 72 hrs by MTT assay Antiproliferative activity against human HL60 cells treated for 24 hrs measured after 72 hrs by MTT assay	[PMID: 29037951]
CCRF-CEM	IC₅₀	21 3 Compound: Chlorambucil	In vitro antiproliferative activity against human CCRF-CEM cells assessed as decrease in cell viability after 24 hrs by MTT assay In vitro antiproliferative activity against human CCRF-CEM cells assessed as decrease in cell viability after 24 hrs by MTT assay	[PMID: 24681986]
HL-60	IC₅₀	38 3 Compound: Chlorambucil	In vitro antiproliferative activity against human HL60 cells assessed as decrease in cell viability after 24 hrs by MTT assay In vitro antiproliferative activity against human HL60 cells assessed as decrease in cell viability after 24 hrs by MTT assay	[PMID: 24681986]
Hs-578T	IC₅₀	162 3 Compound: 1	Cytotoxicity against human Hs578T cells after 72 hrs by MTT assay Cytotoxicity against human Hs578T cells after 72 hrs by MTT assay	[PMID: 24147900]
CH1	IC₅₀	3.3 3 Compound: chlorambucil	Growth inhibition against CH-1 wild-type ovarian cell lines Growth inhibition against CH-1 wild-type ovarian cell lines	[PMID: 15027879]
CH1	IC₅₀	7.9 3 Compound: chlorambucil	Growth inhibition against CH-1 cisplatin resistant wild-type ovarian cell lines Growth inhibition against CH-1 cisplatin resistant wild-type ovarian cell lines	[PMID: 15027879]
HT-29	GI₅₀	5 3 Compound: 1	Growth inhibition of human HT-29 cells after 7 days by celltiter-glo assay Growth inhibition of human HT-29 cells after 7 days by celltiter-glo assay	[PMID: 20973561]
CHO	IC₅₀	30 3 Compound: Chlorambucil	Dose required to inhibit cell growth was determined against CHO cell line Dose required to inhibit cell growth was determined against CHO cell line	[PMID: 11262080]
HT-29	GI₅₀	129 3 Compound: Chlorambucil	Tested for in vitro concentration required to inhibit growth by 50% against HT-29 human colon cancer cell line Tested for in vitro concentration required to inhibit growth by 50% against HT-29 human colon cancer cell line	[PMID: 11755363]
CHO	IC₅₀	84.5 3 Compound: CA, chlorambucil	Cytotoxicity against PAT active CHO cells after 48 hrs Cytotoxicity against PAT active CHO cells after 48 hrs	[PMID: 18281932]
HT-29	IC₅₀	70 3 Compound: 3	Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 19402667]
HT-29	IC₅₀	>100 3 Compound: Chlorambucil	Dose required to inhibit cell growth was determined against HT-29 cell line of human adenocarcinoma Dose required to inhibit cell growth was determined against HT-29 cell line of human adenocarcinoma	[PMID: 11262080]
MCF7	IC₅₀	26.4 1 Compound: Chlorambucil	Cytotoxicity against human MCF7 cells assessed as cell viability after 5 days by cell viability analyzer Cytotoxicity against human MCF7 cells assessed as cell viability after 5 days by cell viability analyzer	[PMID: 26291039]
IMR-32	IC₅₀	20.09 6 Compound: Chlorambucil	Cytotoxicity against human IMR32 cells after 72 hrs by MTT assay Cytotoxicity against human IMR32 cells after 72 hrs by MTT assay	10.1007/s00044-013-0582-8
CHO-AA8	IC₅₀	26 3 Compound: 2 (chlorambucil)	Tested for the in vitro cytotoxicity against transformed chinese hamster fibroblast line AA8 Tested for the in vitro cytotoxicity against transformed chinese hamster fibroblast line AA8	[PMID: 2319563]
IMR-32	IC₅₀	18.8 3 Compound: Chlorambucil	Cytotoxicity against human IMR32 cells assessed as reduction in cell viability after 3 days by MTT assay Cytotoxicity against human IMR32 cells assessed as reduction in cell viability after 3 days by MTT assay	[PMID: 29649739]
JIMT-1	CC₅₀	>150 3 Compound: Chlorambucil	Cytotoxicity against human JIMT1 cells assessed as decrease in cell viability after 48 hrs by MTS assay Cytotoxicity against human JIMT1 cells assessed as decrease in cell viability after 48 hrs by MTS assay	[PMID: 29043812]
CHO-AA8	IC₅₀	26 3 Compound: Chlorambucil	Inhibition of cell growth in culture against Chinese hamster ovary derived cell line, AA8 Inhibition of cell growth in culture against Chinese hamster ovary derived cell line, AA8	[PMID: 2033580]
CHO-AA8	IC₅₀	26 3 Compound: Chlorambucil	Cytotoxicity against AA8 cell line Cytotoxicity against AA8 cell line	[PMID: 2231599]
JIMT-1	CC₅₀	>150 3 Compound: Chlorambucil	Cytotoxicity against human JIMT-1 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Cytotoxicity against human JIMT-1 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 29126728]
K562	IC₅₀	103 3 Compound: Chlorambucil	Anticancer activity against human K562 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Anticancer activity against human K562 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 32527536]
CHO-AA8	IC₅₀	26 3 Compound: cholambucil	Compound was evaluated for in vitro cytotoxicities against Chinese hamster ovary derived AA8 cell lines. Compound was evaluated for in vitro cytotoxicities against Chinese hamster ovary derived AA8 cell lines.	[PMID: 1992124]
CHO-AA8	IC₅₀	36 1 Compound: Chlorambucil	Inhibitory activity of compound for AA8 cells to reduce cell density by 50% (exposed to compound for 4 hours) Inhibitory activity of compound for AA8 cells to reduce cell density by 50% (exposed to compound for 4 hours)	[PMID: 2909741]
K562	IC₅₀	>100 3 Compound: CLB; 3d	Antiproliferative activity against human K562 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human K562 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36240546]
CHO-AA8	IC₅₀	38 3 Compound: 3	Growth inhibition activity was measured in repair proficient AA8 cells under aerobic conditions (4 hours drug exposure) Growth inhibition activity was measured in repair proficient AA8 cells under aerobic conditions (4 hours drug exposure)	[PMID: 7707327]
K562	IC₅₀	60.12 3 Compound: chlorambucil	Antiproliferative activity against human K562 cells assessed as growth inhibition after 72 hrs by MTT assay Antiproliferative activity against human K562 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25050168]
CHO-MG	IC₅₀	111.2 3 Compound: CA, chlorambucil	Cytotoxicity against PAT deficient CHO-MG cells after 48 hrs Cytotoxicity against PAT deficient CHO-MG cells after 48 hrs	[PMID: 18281932]
K562	IC₅₀	95 3 Compound: chlorambucil	Cytotoxicity against human leukemia cell line K562 Cytotoxicity against human leukemia cell line K562	[PMID: 15341486]
COLO 205	IC₅₀	18.8 3 Compound: Chlorambucil	Cytotoxicity against human COLO205 cells assessed as reduction in cell viability after 3 days by MTT assay Cytotoxicity against human COLO205 cells assessed as reduction in cell viability after 3 days by MTT assay	[PMID: 29649739]
K562	IC₅₀	35 3 Compound: Chlorambucil	Cytotoxicity against K562 leukemia cells in vitro, expressed as the minimal concentration necessary to inhibit incorporation of [3H]thymidine by 50% Cytotoxicity against K562 leukemia cells in vitro, expressed as the minimal concentration necessary to inhibit incorporation of [3H]thymidine by 50%	[PMID: 12565950]
COLO 205	IC₅₀	26.67 6 Compound: Chlorambucil	Cytotoxicity against human COLO205 cells after 72 hrs by MTT assay Cytotoxicity against human COLO205 cells after 72 hrs by MTT assay	10.1007/s00044-013-0582-8
K562	IC₅₀	11 3 Compound: Chlorambucil	Dose required to inhibit cell growth was determined against K562 cell line of chronic myelogenous leukemia Dose required to inhibit cell growth was determined against K562 cell line of chronic myelogenous leukemia	[PMID: 11262080]
COS-7	IC₅₀	43.51 3 Compound: Chlorambucil	Cytotoxicity against African green monkey COS7 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Cytotoxicity against African green monkey COS7 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 32527536]
K562	IC₅₀	84 3 Compound: Chlorambucil	In vitro antiproliferative activity against human K562 cells assessed as decrease in cell viability after 24 hrs by MTT assay In vitro antiproliferative activity against human K562 cells assessed as decrease in cell viability after 24 hrs by MTT assay	[PMID: 24681986]
CHO-AA8	IC₅₀	36 1 Compound: Chlorambucil	Inhibitory activity of compound for AA8 cells to reduce cell density by 50% (exposed to compound for 4 hours) Inhibitory activity of compound for AA8 cells to reduce cell density by 50% (exposed to compound for 4 hours)	[PMID: 2909741]
CT26	IC₅₀	> 1000 1 Compound: 2; CLB	Antitumor activity against mouse CT26 cells assessed as inhibition of cell viability incubated for 72 hrs by SRB assay Antitumor activity against mouse CT26 cells assessed as inhibition of cell viability incubated for 72 hrs by SRB assay	[PMID: 38060985]
KYSE-70 cell line	IC₅₀	38.4 3 Compound: Chlorambucil	Antiproliferative activity against human KYSE70 cells after 96 hrs by crystal violet assay Antiproliferative activity against human KYSE70 cells after 96 hrs by crystal violet assay	[PMID: 18434163]
DAN-G	IC₅₀	27.8 3 Compound: Chlo	Cytotoxicity against human DAN-G cells assessed as growth inhibition after 96 hrs by crystal violet staining assay Cytotoxicity against human DAN-G cells assessed as growth inhibition after 96 hrs by crystal violet staining assay	10.1039/C3MD00252G
L02	IC₅₀	>100 3 Compound: 12	Antiproliferative activity against human LO2 cells after 72 hrs by MTT assay Antiproliferative activity against human LO2 cells after 72 hrs by MTT assay	[PMID: 29407983]
DLD-1	IC₅₀	47 3 Compound: 2, Chlorambucil, CLB	Cytotoxicity against human DLD1 cells by resazurin reduction test Cytotoxicity against human DLD1 cells by resazurin reduction test	[PMID: 18424156]
L02	IC₅₀	>80 3 Compound: Chlorambucil	Antiproliferative activity against human LO2 cells after 72 hrs by MTT assay Antiproliferative activity against human LO2 cells after 72 hrs by MTT assay	[PMID: 29524728]
DU-145	CC₅₀	> 150 3 Compound: Chlorambucil	Cytotoxicity against human DU145 cells assessed as decrease in cell viability after 48 hrs by MTS assay Cytotoxicity against human DU145 cells assessed as decrease in cell viability after 48 hrs by MTS assay	[PMID: 29043812]
L02	IC₅₀	>600 3 Compound: CLB	Cytotoxicity against human L02 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Cytotoxicity against human L02 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 28818459]
DU-145	CC₅₀	> 150 3 Compound: Chlorambucil	Cytotoxicity against human DU-145 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Cytotoxicity against human DU-145 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 29126728]
L02	IC₅₀	105.78 3 Compound: chlorambucil	Cytotoxicity against human L02 cells by MTT assay Cytotoxicity against human L02 cells by MTT assay	[PMID: 25050168]
DU-145	GI₅₀	9.4 3 Compound: 1	Growth inhibition of human DU145 cells after 7 days by celltiter-glo assay Growth inhibition of human DU145 cells after 7 days by celltiter-glo assay	[PMID: 20973561]
L02	IC₅₀	>50 3 Compound: Chlorambucil	Cytotoxicity against human LO2 cells assessed as decrease in cell viability after 72 hrs by MTT assay Cytotoxicity against human LO2 cells assessed as decrease in cell viability after 72 hrs by MTT assay	[PMID: 30243153]
L1210	IC₅₀	14.1 3 Compound: CA, chlorambucil	Cytotoxicity against mouse L1210 cells after 48 hrs Cytotoxicity against mouse L1210 cells after 48 hrs	[PMID: 18281932]
Daoy	IC₅₀	1.36 3 Compound: C5	Cytotoxicity against human DaOY cells after 5 days by MTT assay Cytotoxicity against human DaOY cells after 5 days by MTT assay	[PMID: 19121874]
L1210	IC₅₀	3 3 Compound: Chlorambucil	Dose required to inhibit cell growth was determined against L1210 cell line of murine lymphocytotic leukemia Dose required to inhibit cell growth was determined against L1210 cell line of murine lymphocytotic leukemia	[PMID: 11262080]
Fibroblast	IC₅₀	99 3 Compound: 2, Chlorambucil, CLB	Cytotoxicity against human primary fibroblasts by resazurin reduction test Cytotoxicity against human primary fibroblasts by resazurin reduction test	[PMID: 18424156]
Fibroblast	IC₅₀	99 3 Compound: CLB	Cytotoxicity against human fibroblasts assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test Cytotoxicity against human fibroblasts assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test	[PMID: 28927903]
LCLC-103H cell line	IC₅₀	14.5 3 Compound: Chlo	Cytotoxicity against human LCLC-103H cells assessed as growth inhibition after 96 hrs by crystal violet staining assay Cytotoxicity against human LCLC-103H cells assessed as growth inhibition after 96 hrs by crystal violet staining assay	10.1039/C3MD00252G
LN-18	IC₅₀	>1 3 Compound: 2; CLB	Antitumor activity against human LN-18 cells assessed as inhibition of cell viability incubated for 72 hrs by SRB assay Antitumor activity against human LN-18 cells assessed as inhibition of cell viability incubated for 72 hrs by SRB assay	[PMID: 38060985]
HCT-116	GI₅₀	105 3 Compound: Chlorambucil	Tested for in vitro concentration required to inhibit growth by 50% against HCT116 human colon cancer cell line Tested for in vitro concentration required to inhibit growth by 50% against HCT116 human colon cancer cell line	[PMID: 11755363]
LNCaP	IC₅₀	124.3 3 Compound: Chlorambucil	Cytotoxicity against human LNCAP cells expressing androgen receptor after 72 hrs by MTT assay Cytotoxicity against human LNCAP cells expressing androgen receptor after 72 hrs by MTT assay	[PMID: 23665800]
HCT-116	IC₅₀	57.6 3 Compound: Chlorambucil	Antiproliferative activity against human HCT116 cells after 72 hrs by CCK8 assay Antiproliferative activity against human HCT116 cells after 72 hrs by CCK8 assay	[PMID: 28818449]
LoVo	IC₅₀	9 3 Compound: Chlorambucil	Dose to inhibit cell growth determined against LoVo cell line of human adenocarcinoma Dose to inhibit cell growth determined against LoVo cell line of human adenocarcinoma	[PMID: 11262080]
M4Beu cell line	IC₅₀	>100 3 Compound: CLB	Cytotoxicity against human M4Beu assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test Cytotoxicity against human M4Beu assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test	[PMID: 28927903]
HCT-116	IC₅₀	> 40 3 Compound: CLB; 3d	Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36240546]
HCT-15	GI₅₀	8.1 3 Compound: 1	Growth inhibition of human HCT15 cells after 7 days by celltiter-glo assay Growth inhibition of human HCT15 cells after 7 days by celltiter-glo assay	[PMID: 20973561]
M4Beu cell line	IC₅₀	>100 3 Compound: 2, Chlorambucil, CLB	Cytotoxicity against human M4Beu cells by resazurin reduction test Cytotoxicity against human M4Beu cells by resazurin reduction test	[PMID: 18424156]
HCT-15	IC₅₀	3.11 3 Compound: Chlorambucil	Anticancer activity against human HCT15 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Anticancer activity against human HCT15 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 32527536]
M4Beu cell line	IC₅₀	5.1 3 Compound: 1	In vitro cytotoxic activity against M4 Beu cells, by colony forming assay In vitro cytotoxic activity against M4 Beu cells, by colony forming assay	[PMID: 11985479]
HCT-15	IC₅₀	49700 1 Compound: Chl	Cytotoxicity against human HCT15 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human HCT15 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 30996795]
MCF7	GI₅₀	8.2 3 Compound: 1	Growth inhibition of human MCF7 cells after 7 days by celltiter-glo assay Growth inhibition of human MCF7 cells after 7 days by celltiter-glo assay	[PMID: 20973561]
HEK293	IC₅₀	25 3 Compound: CLB	Antiproliferative activity against HEK293 cells after 72 hrs at 37 degC by CellTiter-Glo luminescence assay Antiproliferative activity against HEK293 cells after 72 hrs at 37 degC by CellTiter-Glo luminescence assay	10.1039/C6MD00271D
MCF7	GI₅₀	30 3 Compound: Chlorambucil	Tested for in vitro concentration required to inhibit growth by 50% against MCF-7 human breast cancer cell line cell line Tested for in vitro concentration required to inhibit growth by 50% against MCF-7 human breast cancer cell line cell line	[PMID: 11755363]
HL-60	IC₅₀	11.63 3 Compound: 12	Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay	[PMID: 29407983]
MCF7	IC₅₀	>100 3 Compound: Chlorambucil	Anticancer activity against human MCF7 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Anticancer activity against human MCF7 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 32527536]
HL-60	IC₅₀	11.63 3 Compound: Chlorambucil	Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay	[PMID: 29524728]
MCF7	IC₅₀	>50 3 Compound: Chlorambucil	Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 30243153]
HL-60	IC₅₀	12.87 3 Compound: Chlorambucil	Antiproliferative activity against human HL60 cells treated for 24 hrs measured after 72 hrs by MTT assay Antiproliferative activity against human HL60 cells treated for 24 hrs measured after 72 hrs by MTT assay	[PMID: 29037951]
MCF7	IC₅₀	18.4 3 Compound: Chlorambucil	Antiproliferative activity against human MCF7 cells after 96 hrs by crystal violet assay Antiproliferative activity against human MCF7 cells after 96 hrs by crystal violet assay	[PMID: 18434163]
MCF7	IC₅₀	97 3 Compound: Chlorambucil	Antiproliferative activity against human MCF7 cells assessed as cell viability pre-incubated for 24 hrs with CO2 followed by compound addition after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as cell viability pre-incubated for 24 hrs with CO2 followed by compound addition after 72 hrs by MTT assay	[PMID: 26680364]
HL-60	IC₅₀	21.1 3 Compound: Chlorambucil	Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition by MTT assay Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition by MTT assay	[PMID: 37875056]
MCF7	IC₅₀	80.54 3 Compound: chlorambucil	Antiproliferative activity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25050168]
HL-60	IC₅₀	34.392 3 Compound: CLB; 3d	Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36240546]
HL-60	IC₅₀	38 3 Compound: Chlorambucil	In vitro antiproliferative activity against human HL60 cells assessed as decrease in cell viability after 24 hrs by MTT assay In vitro antiproliferative activity against human HL60 cells assessed as decrease in cell viability after 24 hrs by MTT assay	[PMID: 24681986]
MCF7	IC₅₀	69.33 3 Compound: Chlorambucil	Antiproliferative activity against human MCF7 cells incubated for 72 hrs by SRB assay Antiproliferative activity against human MCF7 cells incubated for 72 hrs by SRB assay	[PMID: 31082763]
HT-29	GI₅₀	129 3 Compound: Chlorambucil	Tested for in vitro concentration required to inhibit growth by 50% against HT-29 human colon cancer cell line Tested for in vitro concentration required to inhibit growth by 50% against HT-29 human colon cancer cell line	[PMID: 11755363]
MCF7	IC₅₀	29.14 3 Compound: Chlorambucil	Antiproliferative activity in human MCF7 cells assessed as inhibition of cell proliferation Antiproliferative activity in human MCF7 cells assessed as inhibition of cell proliferation	[PMID: 29046238]
HT-29	GI₅₀	5 3 Compound: 1	Growth inhibition of human HT-29 cells after 7 days by celltiter-glo assay Growth inhibition of human HT-29 cells after 7 days by celltiter-glo assay	[PMID: 20973561]
MCF7	IC₅₀	56 3 Compound: Chlorambucil	Cytostatic activity against human MCF7 cells assessed as decrease in [3H]thymidine incorporation into DNA after 24 hrs by liquid scintillation counting analysis Cytostatic activity against human MCF7 cells assessed as decrease in [3H]thymidine incorporation into DNA after 24 hrs by liquid scintillation counting analysis	[PMID: 24681986]
HT-29	IC₅₀	70 3 Compound: 3	Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 19402667]
MCF7	IC₅₀	56 3 Compound: Chlorambucil	Cytostatic activity against human MCF7 cells assessed as inhibition of DNA synthesis after 24 hrs by [3H]thymidine incorporation assay Cytostatic activity against human MCF7 cells assessed as inhibition of DNA synthesis after 24 hrs by [3H]thymidine incorporation assay	[PMID: 24713308]
HT-29	IC₅₀	> 100 3 Compound: Chlorambucil	Dose required to inhibit cell growth was determined against HT-29 cell line of human adenocarcinoma Dose required to inhibit cell growth was determined against HT-29 cell line of human adenocarcinoma	[PMID: 11262080]
MCF7	IC₅₀	97 3 Compound: Chlorambucil	Cytotoxic activity against human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxic activity against human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 25921266]
MCF7	IC₅₀	130.36 3 Compound: 3	Cytotoxicity against estrogen receptor-positive human MCF7 cells by MTT assay Cytotoxicity against estrogen receptor-positive human MCF7 cells by MTT assay	[PMID: 21051231]
HeLa	IC₅₀	943 1 Compound: Chlorambucil	Cytotoxicity against human HeLa cells assessed as cell viability after 5 days by cell viability analyzer Cytotoxicity against human HeLa cells assessed as cell viability after 5 days by cell viability analyzer	[PMID: 26291039]
Hep 3B2	GI₅₀	5.1 3 Compound: 1	Growth inhibition of human p53 deficient Hep3B cells after 7 days by celltiter-glo assay Growth inhibition of human p53 deficient Hep3B cells after 7 days by celltiter-glo assay	[PMID: 20973561]
MCF7	IC₅₀	>100 3 Compound: CLB	Cytotoxicity against human MCF7 assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test Cytotoxicity against human MCF7 assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test	[PMID: 28927903]
HepG2	IC₅₀	111.8 3 Compound: Chlorambucil	Antiproliferative activity against human HepG2 cells after 72 hrs by CCK8 assay Antiproliferative activity against human HepG2 cells after 72 hrs by CCK8 assay	[PMID: 28818449]
MCF7	IC₅₀	73.3 3 Compound: 1	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 24147900]
HepG2	IC₅₀	111.8 3 Compound: Chlorambucil	Antiproliferative activity against human HepG2 cells after 72 hrs CCK8 assay Antiproliferative activity against human HepG2 cells after 72 hrs CCK8 assay	[PMID: 30108928]
MCF7	IC₅₀	97 3 Compound: Chlorambucil	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 24713308]
MCF7	IC₅₀	18.4 3 Compound: Chlo	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 96 hrs by crystal violet staining assay Cytotoxicity against human MCF7 cells assessed as growth inhibition after 96 hrs by crystal violet staining assay	10.1039/C3MD00252G
HepG2	IC₅₀	190 3 Compound: CLB	Cytotoxicity against human HepG2 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 28818459]
HepG2	IC₅₀	> 100 3 Compound: Chlorambucil	Antiproliferative activity against human HepG2 cells treated for 24 hrs measured after 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells treated for 24 hrs measured after 72 hrs by MTT assay	[PMID: 29037951]
MCF7	IC₅₀	>100 3 Compound: 2, Chlorambucil, CLB	Cytotoxicity against human MCF7 cells by resazurin reduction test Cytotoxicity against human MCF7 cells by resazurin reduction test	[PMID: 18424156]
MCF7	IC₅₀	4.5 3 Compound: C5	Cytotoxicity against human MCF7 cells expressing estrogen and progesterone receptor after 5 days by MTT assay Cytotoxicity against human MCF7 cells expressing estrogen and progesterone receptor after 5 days by MTT assay	[PMID: 19121874]
MCF7	IC₅₀	97 3 Compound: Chlorambucil	In vitro antiproliferative activity against human MCF7 cells assessed as decrease cell viability after 24 hrs by MTT assay In vitro antiproliferative activity against human MCF7 cells assessed as decrease cell viability after 24 hrs by MTT assay	[PMID: 24681986]
MCF7	IC₅₀	24.6 3 Compound: Chlorambucil	In vitro inhibitory activity against mammary carcinoma (MCF-7) cell line. In vitro inhibitory activity against mammary carcinoma (MCF-7) cell line.	[PMID: 9667961]
MCF7	IC₅₀	52 3 Compound: Chlorambucil	Inhibition of collagen synthesis in human MCF7 cells assessed as 5-[3H]-proline incorporation into proteins susceptible to action of bacterial collagenase after 24 hrs Inhibition of collagen synthesis in human MCF7 cells assessed as 5-[3H]-proline incorporation into proteins susceptible to action of bacterial collagenase after 24 hrs	[PMID: 24713308]
MCF7	IC₅₀	56 3 Compound: Chlorambucil	Inhibition of DNA synthesis in human MCF7 cells assessed as reduction in [3H]-thymidine incorporation incubated for 24 hrs by liquid scintillation counting method Inhibition of DNA synthesis in human MCF7 cells assessed as reduction in [3H]-thymidine incorporation incubated for 24 hrs by liquid scintillation counting method	[PMID: 25921266]
MCF7S	IC₅₀	1.7 3 Compound: 1	In vitro cytotoxic activity against MCF7, by colony forming assay In vitro cytotoxic activity against MCF7, by colony forming assay	[PMID: 11985479]
MDA-MB-231	CC₅₀	>150 3 Compound: Chlorambucil	Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTS assay Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTS assay	10.1039/C6MD00328A
HepG2/Adm	IC₅₀	297.1 3 Compound: CLB	Cytotoxicity against human HepG2/ADM cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Cytotoxicity against human HepG2/ADM cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 28818459]
MDA-MB-231	CC₅₀	>150 3 Compound: Chlorambucil	Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell viability after 48 hrs by MTS assay Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell viability after 48 hrs by MTS assay	[PMID: 29043812]
Hs-578T	IC₅₀	162 3 Compound: 1	Cytotoxicity against human Hs578T cells after 72 hrs by MTT assay Cytotoxicity against human Hs578T cells after 72 hrs by MTT assay	[PMID: 24147900]
MDA-MB-231	CC₅₀	>150 3 Compound: Chlorambucil	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 29126728]
MDA-MB-231	GI₅₀	>25 3 Compound: CLB	Growth inhibition of human MDA-MB-231 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human MDA-MB-231 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
IMR-32	IC₅₀	18.8 3 Compound: Chlorambucil	Cytotoxicity against human IMR32 cells assessed as reduction in cell viability after 3 days by MTT assay Cytotoxicity against human IMR32 cells assessed as reduction in cell viability after 3 days by MTT assay	[PMID: 29649739]
IMR-32	IC₅₀	20.09 6 Compound: Chlorambucil	Cytotoxicity against human IMR32 cells after 72 hrs by MTT assay Cytotoxicity against human IMR32 cells after 72 hrs by MTT assay	10.1007/s00044-013-0582-8
MDA-MB-231	IC₅₀	>130 3 Compound: 2	Anticancer activity against human MDA-MB-231 cells assessed as cell survival after 72 hrs by MTT assay Anticancer activity against human MDA-MB-231 cells assessed as cell survival after 72 hrs by MTT assay	[PMID: 20137939]
JIMT-1	CC₅₀	> 150 3 Compound: Chlorambucil	Cytotoxicity against human JIMT1 cells assessed as decrease in cell viability after 48 hrs by MTS assay Cytotoxicity against human JIMT1 cells assessed as decrease in cell viability after 48 hrs by MTS assay	[PMID: 29043812]
MDA-MB-231	IC₅₀	>100 3 Compound: Chlorambucil	Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by MTT assay	[PMID: 28129978]
MDA-MB-231	IC₅₀	49 3 Compound: Chlorambucil	Cytostatic activity against human MDA-MB-231 cells assessed as decrease in [3H]thymidine incorporation into DNA after 24 hrs by liquid scintillation counting analysis Cytostatic activity against human MDA-MB-231 cells assessed as decrease in [3H]thymidine incorporation into DNA after 24 hrs by liquid scintillation counting analysis	[PMID: 24681986]
JIMT-1	CC₅₀	> 150 3 Compound: Chlorambucil	Cytotoxicity against human JIMT-1 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Cytotoxicity against human JIMT-1 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 29126728]
K562	IC₅₀	103 3 Compound: Chlorambucil	Anticancer activity against human K562 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Anticancer activity against human K562 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 32527536]
MDA-MB-231	IC₅₀	49 3 Compound: Chlorambucil	Cytostatic activity against human MDA-MB-231 cells assessed as inhibition of DNA synthesis after 24 hrs by [3H]thymidine incorporation assay Cytostatic activity against human MDA-MB-231 cells assessed as inhibition of DNA synthesis after 24 hrs by [3H]thymidine incorporation assay	[PMID: 24713308]
MDA-MB-231	IC₅₀	93 3 Compound: Chlorambucil	Cytotoxic activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxic activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 25921266]
K562	IC₅₀	11 3 Compound: Chlorambucil	Dose required to inhibit cell growth was determined against K562 cell line of chronic myelogenous leukemia Dose required to inhibit cell growth was determined against K562 cell line of chronic myelogenous leukemia	[PMID: 11262080]
MDA-MB-231	IC₅₀	136.85 3 Compound: 3	Cytotoxicity against estrogen receptor-negative human MDA-MB-231 cells by MTT assay Cytotoxicity against estrogen receptor-negative human MDA-MB-231 cells by MTT assay	[PMID: 21051231]
K562	IC₅₀	35 3 Compound: Chlorambucil	Cytotoxicity against K562 leukemia cells in vitro, expressed as the minimal concentration necessary to inhibit incorporation of [3H]thymidine by 50% Cytotoxicity against K562 leukemia cells in vitro, expressed as the minimal concentration necessary to inhibit incorporation of [3H]thymidine by 50%	[PMID: 12565950]
K562	IC₅₀	60.12 3 Compound: chlorambucil	Antiproliferative activity against human K562 cells assessed as growth inhibition after 72 hrs by MTT assay Antiproliferative activity against human K562 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25050168]
MDA-MB-231	IC₅₀	93 3 Compound: Chlorambucil	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 24713308]
MDA-MB-231	IC₅₀	>200 3 Compound: 1	Cytotoxicity against mitochondrial DNA-deficient human MDA-MB-231 rho theta-W02 cells after 72 hrs by MTT assay Cytotoxicity against mitochondrial DNA-deficient human MDA-MB-231 rho theta-W02 cells after 72 hrs by MTT assay	[PMID: 24147900]
K562	IC₅₀	84 3 Compound: Chlorambucil	In vitro antiproliferative activity against human K562 cells assessed as decrease in cell viability after 24 hrs by MTT assay In vitro antiproliferative activity against human K562 cells assessed as decrease in cell viability after 24 hrs by MTT assay	[PMID: 24681986]
MDA-MB-231	IC₅₀	80 3 Compound: Chlorambucil	Dose required to inhibit cell growth was determined against MDA-MB-231 cell line of human non-hormone dependent breast carcinoma Dose required to inhibit cell growth was determined against MDA-MB-231 cell line of human non-hormone dependent breast carcinoma	[PMID: 11262080]
K562	IC₅₀	95 3 Compound: chlorambucil	Cytotoxicity against human leukemia cell line K562 Cytotoxicity against human leukemia cell line K562	[PMID: 15341486]
K562	IC₅₀	> 100 3 Compound: CLB; 3d	Antiproliferative activity against human K562 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human K562 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36240546]
MDA-MB-231	IC₅₀	93 3 Compound: Chlorambucil	In vitro antiproliferative activity against human MDA-MB-231 cells assessed as decrease in cell vaibility after 24 hrs by MTT assay In vitro antiproliferative activity against human MDA-MB-231 cells assessed as decrease in cell vaibility after 24 hrs by MTT assay	[PMID: 24681986]
MDA-MB-231	IC₅₀	72 3 Compound: Chlorambucil	Inhibition of collagen synthesis in human MDA-MB-231 cells assessed as 5-[3H]-proline incorporation into proteins susceptible to action of bacterial collagenase after 24 hrs Inhibition of collagen synthesis in human MDA-MB-231 cells assessed as 5-[3H]-proline incorporation into proteins susceptible to action of bacterial collagenase after 24 hrs	[PMID: 24713308]
L02	IC₅₀	105.78 3 Compound: chlorambucil	Cytotoxicity against human L02 cells by MTT assay Cytotoxicity against human L02 cells by MTT assay	[PMID: 25050168]
L02	IC₅₀	> 100 3 Compound: 12	Antiproliferative activity against human LO2 cells after 72 hrs by MTT assay Antiproliferative activity against human LO2 cells after 72 hrs by MTT assay	[PMID: 29407983]
MDA-MB-231	IC₅₀	49 3 Compound: Chlorambucil	Inhibition of DNA synthesis in human MDA-MB-231 cells assessed as reduction in [3H]-thymidine incorporation incubated for 24 hrs by liquid scintillation counting method Inhibition of DNA synthesis in human MDA-MB-231 cells assessed as reduction in [3H]-thymidine incorporation incubated for 24 hrs by liquid scintillation counting method	[PMID: 25921266]
MDA-MB-435	IC₅₀	>200 3 Compound: 1	Cytotoxicity against mitochondrial DNA-deficient human MDA-MB-435 rho theta-W01 cells after 72 hrs by MTT assay Cytotoxicity against mitochondrial DNA-deficient human MDA-MB-435 rho theta-W01 cells after 72 hrs by MTT assay	[PMID: 24147900]
L02	IC₅₀	> 50 3 Compound: Chlorambucil	Cytotoxicity against human LO2 cells assessed as decrease in cell viability after 72 hrs by MTT assay Cytotoxicity against human LO2 cells assessed as decrease in cell viability after 72 hrs by MTT assay	[PMID: 30243153]
L02	IC₅₀	> 600 3 Compound: CLB	Cytotoxicity against human L02 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Cytotoxicity against human L02 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 28818459]
MDA-MB-435	IC₅₀	150 3 Compound: 1	Cytotoxicity against wild type human MDA-MB-435 cells after 72 hrs by MTT assay Cytotoxicity against wild type human MDA-MB-435 cells after 72 hrs by MTT assay	[PMID: 24147900]
L02	IC₅₀	> 80 3 Compound: Chlorambucil	Antiproliferative activity against human LO2 cells after 72 hrs by MTT assay Antiproliferative activity against human LO2 cells after 72 hrs by MTT assay	[PMID: 29524728]
MDA-MB-436	IC₅₀	>160 3 Compound: 2	Anticancer activity against human MDA-MB-436 cells assessed as cell survival after 72 hrs by MTT assay Anticancer activity against human MDA-MB-436 cells assessed as cell survival after 72 hrs by MTT assay	[PMID: 20137939]
MDA-MB-436	IC₅₀	131.83 3 Compound: 3	Cytotoxicity against estrogen receptor-negative human MDA-MB-436 cells by MTT assay Cytotoxicity against estrogen receptor-negative human MDA-MB-436 cells by MTT assay	[PMID: 21051231]
L1210	IC₅₀	14.1 3 Compound: CA, chlorambucil	Cytotoxicity against mouse L1210 cells after 48 hrs Cytotoxicity against mouse L1210 cells after 48 hrs	[PMID: 18281932]
MDA-MB-468	IC₅₀	34.4 3 Compound: Chlorambucil	Anticancer activity against human MDA-MB-468 cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay Anticancer activity against human MDA-MB-468 cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay	[PMID: 28388522]
L1210	IC₅₀	3 3 Compound: Chlorambucil	Dose required to inhibit cell growth was determined against L1210 cell line of murine lymphocytotic leukemia Dose required to inhibit cell growth was determined against L1210 cell line of murine lymphocytotic leukemia	[PMID: 11262080]
MDA-MB-468	IC₅₀	146.88 3 Compound: 3	Cytotoxicity against estrogen receptor-negative human MDA-MB-468 cells by MTT assay Cytotoxicity against estrogen receptor-negative human MDA-MB-468 cells by MTT assay	[PMID: 21051231]
MDA-MB-468	IC₅₀	43.8 3 Compound: 1	Cytotoxicity against human MDA-MB-468 cells after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-468 cells after 72 hrs by MTT assay	[PMID: 24147900]
MDA-MB-468	IC₅₀	67.5 3 Compound: 1	Cytotoxicity against human MDA-MB-468 cells after 72 hrs by MTT assay in presence of NAC Cytotoxicity against human MDA-MB-468 cells after 72 hrs by MTT assay in presence of NAC	[PMID: 24147900]
MDA-MB-468	IC₅₀	0.65 3 Compound: C5	Cytotoxicity against human MDA-MB-468 cells expressing estrogen receptor after 5 days by MTT assay Cytotoxicity against human MDA-MB-468 cells expressing estrogen receptor after 5 days by MTT assay	[PMID: 19121874]
MDA-MB-468	IC₅₀	0.79 3 Compound: C5	Cytotoxicity against human MDA-MB-468 cells transfected with NAD(P)H: quinone oxidoreductase NQO1 after 5 days by MTT assay Cytotoxicity against human MDA-MB-468 cells transfected with NAD(P)H: quinone oxidoreductase NQO1 after 5 days by MTT assay	[PMID: 19121874]
MDA-MB-468	IC₅₀	34.4 3 Compound: Chlorambucil	Growth inhibition of human MDA-MB-468 cells after 48 hrs by fluorescence polarisation assay Growth inhibition of human MDA-MB-468 cells after 48 hrs by fluorescence polarisation assay	[PMID: 30247905]
A549	IC₅₀	85.7 1 Compound: Chlorambucil	Cytotoxicity against human A549 cells assessed as cell viability after 5 days by cell viability analyzer Cytotoxicity against human A549 cells assessed as cell viability after 5 days by cell viability analyzer	[PMID: 26291039]
LN-18	IC₅₀	> 1000 1 Compound: 2; CLB	Antitumor activity against human LN-18 cells assessed as inhibition of cell viability incubated for 72 hrs by SRB assay Antitumor activity against human LN-18 cells assessed as inhibition of cell viability incubated for 72 hrs by SRB assay	[PMID: 38060985]
MDCK	IC₅₀	100 3 Compound: Chlorambucil	Cytotoxicity against carbonic anhydrase 9 knockdown MDCK cells assessed as reduction in cell viability after 2 hrs under hypoxic condition by Alamar blue assay Cytotoxicity against carbonic anhydrase 9 knockdown MDCK cells assessed as reduction in cell viability after 2 hrs under hypoxic condition by Alamar blue assay	[PMID: 27823879]
LNCaP	IC₅₀	124.3 3 Compound: Chlorambucil	Cytotoxicity against human LNCAP cells expressing androgen receptor after 72 hrs by MTT assay Cytotoxicity against human LNCAP cells expressing androgen receptor after 72 hrs by MTT assay	[PMID: 23665800]
LoVo	IC₅₀	9 3 Compound: Chlorambucil	Dose to inhibit cell growth determined against LoVo cell line of human adenocarcinoma Dose to inhibit cell growth determined against LoVo cell line of human adenocarcinoma	[PMID: 11262080]
MDCK	IC₅₀	93 3 Compound: Chlorambucil	Cytotoxicity against carbonic anhydrase 9 knockdown MDCK cells assessed as reduction in cell viability preincubated for 2 hrs followed by 72 hrs incubation under normoxic condition by Alamar blue assay Cytotoxicity against carbonic anhydrase 9 knockdown MDCK cells assessed as reduction in cell viability preincubated for 2 hrs followed by 72 hrs incubation under normoxic condition by Alamar blue assay	[PMID: 27823879]
MDCK	IC₅₀	>100 3 Compound: Chlorambucil	Cytotoxicity against MDCK cells expressing carbonic anhydrase 9 assessed as reduction in cell viability after 2 hrs under hypoxic condition by Alamar blue assay Cytotoxicity against MDCK cells expressing carbonic anhydrase 9 assessed as reduction in cell viability after 2 hrs under hypoxic condition by Alamar blue assay	[PMID: 27823879]
MGC-803	IC₅₀	133.64 3 Compound: chlorambucil	Antiproliferative activity against human MGC803 cells assessed as growth inhibition after 72 hrs by MTT assay Antiproliferative activity against human MGC803 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25050168]
MCF7	GI₅₀	30 3 Compound: Chlorambucil	Tested for in vitro concentration required to inhibit growth by 50% against MCF-7 human breast cancer cell line cell line Tested for in vitro concentration required to inhibit growth by 50% against MCF-7 human breast cancer cell line cell line	[PMID: 11755363]
MIA PaCa-2	CC₅₀	>150 3 Compound: Chlorambucil	Cytotoxicity against human MIA PaCa-2 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Cytotoxicity against human MIA PaCa-2 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 29126728]
MCF7	GI₅₀	8.2 3 Compound: 1	Growth inhibition of human MCF7 cells after 7 days by celltiter-glo assay Growth inhibition of human MCF7 cells after 7 days by celltiter-glo assay	[PMID: 20973561]
MIA PaCa-2	CC₅₀	>150 3 Compound: Chlorambucil	Cytotoxicity against human MIAPaCa2 cells assessed as decrease in cell viability after 48 hrs by MTS assay Cytotoxicity against human MIAPaCa2 cells assessed as decrease in cell viability after 48 hrs by MTS assay	[PMID: 29043812]
MCF7	IC₅₀	130.36 3 Compound: 3	Cytotoxicity against estrogen receptor-positive human MCF7 cells by MTT assay Cytotoxicity against estrogen receptor-positive human MCF7 cells by MTT assay	[PMID: 21051231]
MIA PaCa-2	IC₅₀	>100 3 Compound: Chlorambucil	Antiproliferative activity against human MIAPaCa2 cells after 72 hrs by MTT assay Antiproliferative activity against human MIAPaCa2 cells after 72 hrs by MTT assay	[PMID: 28129978]
MIA PaCa-2	IC₅₀	78.3 3 Compound: 1	Cytotoxicity against human MIAPaCa2 cells after 72 hrs by MTT assay Cytotoxicity against human MIAPaCa2 cells after 72 hrs by MTT assay	[PMID: 24147900]
MCF7	IC₅₀	18.4 3 Compound: Chlo	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 96 hrs by crystal violet staining assay Cytotoxicity against human MCF7 cells assessed as growth inhibition after 96 hrs by crystal violet staining assay	10.1039/C3MD00252G
MCF7	IC₅₀	18.4 3 Compound: Chlorambucil	Antiproliferative activity against human MCF7 cells after 96 hrs by crystal violet assay Antiproliferative activity against human MCF7 cells after 96 hrs by crystal violet assay	[PMID: 18434163]
MOLT-4	IC₅₀	6.3 3 Compound: Chlorambucil	In vitro inhibitory activity against acute lymphoblastic leukemia (MOLT4) cell line. In vitro inhibitory activity against acute lymphoblastic leukemia (MOLT4) cell line.	[PMID: 9667961]
MV4-11	IC₅₀	8.55 3 Compound: Chlorambucil	Antiproliferative activity against human MV4-11 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human MV4-11 cells incubated for 72 hrs by MTT assay	[PMID: 31082763]
MCF7	IC₅₀	24.6 3 Compound: Chlorambucil	In vitro inhibitory activity against mammary carcinoma (MCF-7) cell line. In vitro inhibitory activity against mammary carcinoma (MCF-7) cell line.	[PMID: 9667961]
MCF7	IC₅₀	26.4 1 Compound: Chlorambucil	Cytotoxicity against human MCF7 cells assessed as cell viability after 5 days by cell viability analyzer Cytotoxicity against human MCF7 cells assessed as cell viability after 5 days by cell viability analyzer	[PMID: 26291039]
NCI/ADR-RES	GI₅₀	56 3 Compound: Chlorambucil	Tested for in vitro concentration required to inhibit growth by 50% against MCF-7/ADR human breast cancer cell line cell line Tested for in vitro concentration required to inhibit growth by 50% against MCF-7/ADR human breast cancer cell line cell line	[PMID: 11755363]
MCF7	IC₅₀	29.14 3 Compound: Chlorambucil	Antiproliferative activity in human MCF7 cells assessed as inhibition of cell proliferation Antiproliferative activity in human MCF7 cells assessed as inhibition of cell proliferation	[PMID: 29046238]
NCI-H1299	GI₅₀	>25 3 Compound: CLB	Growth inhibition of human NCI-H1299 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human NCI-H1299 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
NCI-H1299	GI₅₀	4.9 3 Compound: 1	Growth inhibition of human p53 deficient H1299 cells after 7 days by celltiter-glo assay Growth inhibition of human p53 deficient H1299 cells after 7 days by celltiter-glo assay	[PMID: 20973561]
MCF7	IC₅₀	4.5 3 Compound: C5	Cytotoxicity against human MCF7 cells expressing estrogen and progesterone receptor after 5 days by MTT assay Cytotoxicity against human MCF7 cells expressing estrogen and progesterone receptor after 5 days by MTT assay	[PMID: 19121874]
MCF7	IC₅₀	52 3 Compound: Chlorambucil	Inhibition of collagen synthesis in human MCF7 cells assessed as 5-[3H]-proline incorporation into proteins susceptible to action of bacterial collagenase after 24 hrs Inhibition of collagen synthesis in human MCF7 cells assessed as 5-[3H]-proline incorporation into proteins susceptible to action of bacterial collagenase after 24 hrs	[PMID: 24713308]
NCI-H1299	IC₅₀	116.3 3 Compound: Chlorambucil	Antiproliferative activity against human H1299 cells after 72 hrs CCK8 assay Antiproliferative activity against human H1299 cells after 72 hrs CCK8 assay	[PMID: 30108928]
MCF7	IC₅₀	56 3 Compound: Chlorambucil	Cytostatic activity against human MCF7 cells assessed as decrease in [3H]thymidine incorporation into DNA after 24 hrs by liquid scintillation counting analysis Cytostatic activity against human MCF7 cells assessed as decrease in [3H]thymidine incorporation into DNA after 24 hrs by liquid scintillation counting analysis	[PMID: 24681986]
NCI-H1975	IC₅₀	28.16 3 Compound: Chlorambucil	Growth inhibition of human NCI-H1975 cells incubated for 5 days by SRB assay Growth inhibition of human NCI-H1975 cells incubated for 5 days by SRB assay	[PMID: 32145644]
MCF7	IC₅₀	56 3 Compound: Chlorambucil	Cytostatic activity against human MCF7 cells assessed as inhibition of DNA synthesis after 24 hrs by [3H]thymidine incorporation assay Cytostatic activity against human MCF7 cells assessed as inhibition of DNA synthesis after 24 hrs by [3H]thymidine incorporation assay	[PMID: 24713308]
NCI-H460	CC₅₀	104 3 Compound: Chlorambucil	Cytotoxicity against human H460 cells after 24 hrs by MTT assay Cytotoxicity against human H460 cells after 24 hrs by MTT assay	[PMID: 20615582]
MCF7	IC₅₀	56 3 Compound: Chlorambucil	Inhibition of DNA synthesis in human MCF7 cells assessed as reduction in [3H]-thymidine incorporation incubated for 24 hrs by liquid scintillation counting method Inhibition of DNA synthesis in human MCF7 cells assessed as reduction in [3H]-thymidine incorporation incubated for 24 hrs by liquid scintillation counting method	[PMID: 25921266]
NCI-H460	GI₅₀	11 3 Compound: 1	Growth inhibition of human NCI-H460 cells after 7 days by celltiter-glo assay Growth inhibition of human NCI-H460 cells after 7 days by celltiter-glo assay	[PMID: 20973561]
NCI-H460	IC₅₀	28.9 3 Compound: Chlorambucil	Antiproliferative activity against human H460 cells after 72 hrs CCK8 assay Antiproliferative activity against human H460 cells after 72 hrs CCK8 assay	[PMID: 30108928]
MCF7	IC₅₀	69.33 3 Compound: Chlorambucil	Antiproliferative activity against human MCF7 cells incubated for 72 hrs by SRB assay Antiproliferative activity against human MCF7 cells incubated for 72 hrs by SRB assay	[PMID: 31082763]
NCI-H460	IC₅₀	2.19 3 Compound: C5	Cytotoxicity against human H460 cells after 5 days by MTT assay Cytotoxicity against human H460 cells after 5 days by MTT assay	[PMID: 19121874]
MCF7	IC₅₀	73.3 3 Compound: 1	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 24147900]
MCF7	IC₅₀	80.54 3 Compound: chlorambucil	Antiproliferative activity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25050168]
NIH3T3	IC₅₀	>600 3 Compound: CLB	Cytotoxicity against mouse NIH/3T3 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 28818459]
MCF7	IC₅₀	97 3 Compound: Chlorambucil	In vitro antiproliferative activity against human MCF7 cells assessed as decrease cell viability after 24 hrs by MTT assay In vitro antiproliferative activity against human MCF7 cells assessed as decrease cell viability after 24 hrs by MTT assay	[PMID: 24681986]
P388	IC₅₀	7.5 3 Compound: Chlorambucil	Cytotoxicity against P388 leukemia cell line Cytotoxicity against P388 leukemia cell line	[PMID: 2231599]
P388	IC₅₀	7 3 Compound: Chlorambucil	Dose required to inhibit cell growth was determined against P388D1 cell line of murine lymphocytotic leukemia Dose required to inhibit cell growth was determined against P388D1 cell line of murine lymphocytotic leukemia	[PMID: 11262080]
MCF7	IC₅₀	97 3 Compound: Chlorambucil	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 24713308]
MCF7	IC₅₀	97 3 Compound: Chlorambucil	Cytotoxic activity against human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxic activity against human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 25921266]
P388	IC₅₀	6.8 3 Compound: cholambucil	In vitro cytotoxicity against murine leukemia P388 cell line. In vitro cytotoxicity against murine leukemia P388 cell line.	[PMID: 1992124]
MCF7	IC₅₀	97 3 Compound: Chlorambucil	Antiproliferative activity against human MCF7 cells assessed as cell viability pre-incubated for 24 hrs with CO2 followed by compound addition after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as cell viability pre-incubated for 24 hrs with CO2 followed by compound addition after 72 hrs by MTT assay	[PMID: 26680364]
P388	IC₅₀	6.75 3 Compound: Chlorambucil	Inhibition of cell growth in culture against wild type P388 murine leukemia Inhibition of cell growth in culture against wild type P388 murine leukemia	[PMID: 2033580]
MCF7	IC₅₀	> 100 3 Compound: 2, Chlorambucil, CLB	Cytotoxicity against human MCF7 cells by resazurin reduction test Cytotoxicity against human MCF7 cells by resazurin reduction test	[PMID: 18424156]
P388	IC₅₀	225 3 Compound: cholambucil	Optimal dose administered as a single dose by intraperitoneal inoculation of 10 X E6 murine leukemia P388 cell lines. Optimal dose administered as a single dose by intraperitoneal inoculation of 10 X E6 murine leukemia P388 cell lines.	[PMID: 1992124]
MCF7	IC₅₀	> 100 3 Compound: CLB	Cytotoxicity against human MCF7 assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test Cytotoxicity against human MCF7 assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test	[PMID: 28927903]
P388	IC₅₀	37 3 Compound: cholambucil	Percentage increase in lifespan of drug-related tumor bearing animals when heated at the optimal dose and tested in murine leukemia P388 cell lines. Percentage increase in lifespan of drug-related tumor bearing animals when heated at the optimal dose and tested in murine leukemia P388 cell lines.	[PMID: 1992124]
MCF7	IC₅₀	> 100 3 Compound: Chlorambucil	Anticancer activity against human MCF7 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Anticancer activity against human MCF7 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 32527536]
P388	IC₅₀	6.75 3 Compound: 2 (chlorambucil)	Tested for the in vitro cytotoxicity against P388 murine leukemia Tested for the in vitro cytotoxicity against P388 murine leukemia	[PMID: 2319563]
MCF7	IC₅₀	> 50 3 Compound: Chlorambucil	Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 30243153]
PA-1	IC₅₀	2.8 3 Compound: CLB	Cytotoxicity against human PA1 assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test Cytotoxicity against human PA1 assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test	[PMID: 28927903]
MCF7S	IC₅₀	1.7 3 Compound: 1	In vitro cytotoxic activity against MCF7, by colony forming assay In vitro cytotoxic activity against MCF7, by colony forming assay	[PMID: 11985479]
PA-1	IC₅₀	2.8 3 Compound: 2, Chlorambucil, CLB	Cytotoxicity against human PA1 cells by resazurin reduction test Cytotoxicity against human PA1 cells by resazurin reduction test	[PMID: 18424156]
MDA-MB-231	CC₅₀	> 150 3 Compound: Chlorambucil	Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTS assay Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTS assay	10.1039/C6MD00328A
Panel NCI-60 (60 carcinoma cell lines)	GI₅₀	60 3 Compound: 53	Antiproliferative activity against human NCI60 cells after 48 hrs by SRB assay Antiproliferative activity against human NCI60 cells after 48 hrs by SRB assay	[PMID: 29870668]
MDA-MB-231	CC₅₀	> 150 3 Compound: Chlorambucil	Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell viability after 48 hrs by MTS assay Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell viability after 48 hrs by MTS assay	[PMID: 29043812]
PBMC	IC₅₀	>200 3 Compound: Chlorambucil	Antiproliferative activity against human PBMC treated for 24 hrs measured after 72 hrs by MTT assay Antiproliferative activity against human PBMC treated for 24 hrs measured after 72 hrs by MTT assay	[PMID: 29037951]
MDA-MB-231	CC₅₀	> 150 3 Compound: Chlorambucil	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 29126728]
PC-3	CC₅₀	>150 3 Compound: Chlorambucil	Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs by MTS assay Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs by MTS assay	[PMID: 29043812]
PC-3	CC₅₀	>150 3 Compound: Chlorambucil	Cytotoxicity against human PC-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Cytotoxicity against human PC-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 29126728]
MDA-MB-231	GI₅₀	> 25 3 Compound: CLB	Growth inhibition of human MDA-MB-231 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human MDA-MB-231 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
PC-3	GI₅₀	>25 3 Compound: CLB	Growth inhibition of human PC3 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human PC3 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
PC-3	IC₅₀	16.4 3 Compound: Chlorambucil	Anticancer activity against human PC3 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Anticancer activity against human PC3 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 32527536]
MDA-MB-231	GI₅₀	> 25 3 Compound: CLB	Growth inhibition of human MDA-MB-231 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human MDA-MB-231 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
MDA-MB-231	IC₅₀	136.85 3 Compound: 3	Cytotoxicity against estrogen receptor-negative human MDA-MB-231 cells by MTT assay Cytotoxicity against estrogen receptor-negative human MDA-MB-231 cells by MTT assay	[PMID: 21051231]
PC-3	IC₅₀	40.35 3 Compound: 12	Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay	[PMID: 29407983]
MDA-MB-231	IC₅₀	49 3 Compound: Chlorambucil	Cytostatic activity against human MDA-MB-231 cells assessed as decrease in [3H]thymidine incorporation into DNA after 24 hrs by liquid scintillation counting analysis Cytostatic activity against human MDA-MB-231 cells assessed as decrease in [3H]thymidine incorporation into DNA after 24 hrs by liquid scintillation counting analysis	[PMID: 24681986]
PC-3	IC₅₀	40.35 3 Compound: Chlorambucil	Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay	[PMID: 29524728]
PC-3	IC₅₀	42.05 3 Compound: Chlorambucil	Antiproliferative activity against human PC3 cells treated for 24 hrs measured after 72 hrs by MTT assay Antiproliferative activity against human PC3 cells treated for 24 hrs measured after 72 hrs by MTT assay	[PMID: 29037951]
MDA-MB-231	IC₅₀	49 3 Compound: Chlorambucil	Cytostatic activity against human MDA-MB-231 cells assessed as inhibition of DNA synthesis after 24 hrs by [3H]thymidine incorporation assay Cytostatic activity against human MDA-MB-231 cells assessed as inhibition of DNA synthesis after 24 hrs by [3H]thymidine incorporation assay	[PMID: 24713308]
MDA-MB-231	IC₅₀	49 3 Compound: Chlorambucil	Inhibition of DNA synthesis in human MDA-MB-231 cells assessed as reduction in [3H]-thymidine incorporation incubated for 24 hrs by liquid scintillation counting method Inhibition of DNA synthesis in human MDA-MB-231 cells assessed as reduction in [3H]-thymidine incorporation incubated for 24 hrs by liquid scintillation counting method	[PMID: 25921266]
MDA-MB-231	IC₅₀	520 1 Compound: Chlorambucil	Cytotoxicity against human MDA-MB-231 cells assessed as cell viability after 5 days by cell viability analyzer Cytotoxicity against human MDA-MB-231 cells assessed as cell viability after 5 days by cell viability analyzer	[PMID: 26291039]
MDA-MB-231	IC₅₀	520 1 Compound: Chlorambucil	Cytotoxicity against human MDA-MB-231 cells assessed as cell viability after 5 days by cell viability analyzer Cytotoxicity against human MDA-MB-231 cells assessed as cell viability after 5 days by cell viability analyzer	[PMID: 26291039]
PC-3	IC₅₀	131.3 3 Compound: Chlorambucil	Cytotoxicity against androgen receptor deficient human PC3 cells after 72 hrs by MTT assay Cytotoxicity against androgen receptor deficient human PC3 cells after 72 hrs by MTT assay	[PMID: 23665800]
MDA-MB-231	IC₅₀	72 3 Compound: Chlorambucil	Inhibition of collagen synthesis in human MDA-MB-231 cells assessed as 5-[3H]-proline incorporation into proteins susceptible to action of bacterial collagenase after 24 hrs Inhibition of collagen synthesis in human MDA-MB-231 cells assessed as 5-[3H]-proline incorporation into proteins susceptible to action of bacterial collagenase after 24 hrs	[PMID: 24713308]
PC-3	IC₅₀	>100 3 Compound: CLB	Cytotoxicity against human PC3 assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test Cytotoxicity against human PC3 assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test	[PMID: 28927903]
PC-9	IC₅₀	7.17 3 Compound: Chlorambucil	Growth inhibition of human PC-9 cells incubated for 5 days by SRB assay Growth inhibition of human PC-9 cells incubated for 5 days by SRB assay	[PMID: 32145644]
MDA-MB-231	IC₅₀	80 3 Compound: Chlorambucil	Dose required to inhibit cell growth was determined against MDA-MB-231 cell line of human non-hormone dependent breast carcinoma Dose required to inhibit cell growth was determined against MDA-MB-231 cell line of human non-hormone dependent breast carcinoma	[PMID: 11262080]
MDA-MB-231	IC₅₀	93 3 Compound: Chlorambucil	In vitro antiproliferative activity against human MDA-MB-231 cells assessed as decrease in cell vaibility after 24 hrs by MTT assay In vitro antiproliferative activity against human MDA-MB-231 cells assessed as decrease in cell vaibility after 24 hrs by MTT assay	[PMID: 24681986]
Raji	IC₅₀	298 3 Compound: chlorambucil	In vitro cytotoxicity against Raji cells from human lymphoma In vitro cytotoxicity against Raji cells from human lymphoma	10.1016/0960-894X(94)80008-1
MDA-MB-231	IC₅₀	93 3 Compound: Chlorambucil	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 24713308]
HeLa	IC₅₀	943 1 Compound: Chlorambucil	Cytotoxicity against human HeLa cells assessed as cell viability after 5 days by cell viability analyzer Cytotoxicity against human HeLa cells assessed as cell viability after 5 days by cell viability analyzer	[PMID: 26291039]
MDA-MB-231	IC₅₀	93 3 Compound: Chlorambucil	Cytotoxic activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxic activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 25921266]
SF-268	GI₅₀	5.4 3 Compound: 1	Growth inhibition of human SF268 cells after 7 days by celltiter-glo assay Growth inhibition of human SF268 cells after 7 days by celltiter-glo assay	[PMID: 20973561]
SH-SY5Y	IC₅₀	>40 3 Compound: CLB; 3d	Antiproliferative activity against human SH-SY5Y cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human SH-SY5Y cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36240546]
MDA-MB-231	IC₅₀	> 100 3 Compound: Chlorambucil	Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by MTT assay	[PMID: 28129978]
MDA-MB-231	IC₅₀	> 130 3 Compound: 2	Anticancer activity against human MDA-MB-231 cells assessed as cell survival after 72 hrs by MTT assay Anticancer activity against human MDA-MB-231 cells assessed as cell survival after 72 hrs by MTT assay	[PMID: 20137939]
SK-BR-3	IC₅₀	247.5 3 Compound: 1	Cytotoxicity against human SKBR3 cells after 72 hrs by MTT assay Cytotoxicity against human SKBR3 cells after 72 hrs by MTT assay	[PMID: 24147900]
MDA-MB-231	IC₅₀	> 200 3 Compound: 1	Cytotoxicity against mitochondrial DNA-deficient human MDA-MB-231 rho theta-W02 cells after 72 hrs by MTT assay Cytotoxicity against mitochondrial DNA-deficient human MDA-MB-231 rho theta-W02 cells after 72 hrs by MTT assay	[PMID: 24147900]
SK-LU-1	IC₅₀	300 3 Compound: Chlorambucil	Anticancer activity against human SKLU1 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Anticancer activity against human SKLU1 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 32527536]
MDA-MB-231	IC₅₀	> 200 3 Compound: 1	Cytotoxicity against wild type human MDA-MB-231 cells after 72 hrs by MTT assay Cytotoxicity against wild type human MDA-MB-231 cells after 72 hrs by MTT assay	[PMID: 24147900]
SK-OV-3	GI₅₀	5.5 3 Compound: 1	Growth inhibition of human SKOV3 cells after 7 days by celltiter-glo assay Growth inhibition of human SKOV3 cells after 7 days by celltiter-glo assay	[PMID: 20973561]
MDA-MB-435	IC₅₀	150 3 Compound: 1	Cytotoxicity against wild type human MDA-MB-435 cells after 72 hrs by MTT assay Cytotoxicity against wild type human MDA-MB-435 cells after 72 hrs by MTT assay	[PMID: 24147900]
SK-OV-3	IC₅₀	35 3 Compound: chlorambucil	Growth inhibition against SKOV3 wild-type ovarian cell lines Growth inhibition against SKOV3 wild-type ovarian cell lines	[PMID: 15027879]
SMMC-7721	IC₅₀	163 3 Compound: Chlorambucil	Antiproliferative activity against human SMMC7721 cells after 72 hrs CCK8 assay Antiproliferative activity against human SMMC7721 cells after 72 hrs CCK8 assay	[PMID: 30108928]
MDA-MB-435	IC₅₀	> 200 3 Compound: 1	Cytotoxicity against mitochondrial DNA-deficient human MDA-MB-435 rho theta-W01 cells after 72 hrs by MTT assay Cytotoxicity against mitochondrial DNA-deficient human MDA-MB-435 rho theta-W01 cells after 72 hrs by MTT assay	[PMID: 24147900]
MDA-MB-435	IC₅₀	> 200 3 Compound: 1	Cytotoxicity against mitochondrial DNA-deficient human MDA-MB-435 rho theta-W02 cells after 72 hrs by MTT assay Cytotoxicity against mitochondrial DNA-deficient human MDA-MB-435 rho theta-W02 cells after 72 hrs by MTT assay	[PMID: 24147900]
SN12C	IC₅₀	135 3 Compound: Chlorambucil	Anticancer activity against human SN12C cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay Anticancer activity against human SN12C cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay	[PMID: 28388522]
2008	IC₅₀	12500 1 Compound: Chl	Cytotoxicity against human 2008 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human 2008 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 30996795]
MDA-MB-436	IC₅₀	131.83 3 Compound: 3	Cytotoxicity against estrogen receptor-negative human MDA-MB-436 cells by MTT assay Cytotoxicity against estrogen receptor-negative human MDA-MB-436 cells by MTT assay	[PMID: 21051231]
MDA-MB-436	IC₅₀	> 160 3 Compound: 2	Anticancer activity against human MDA-MB-436 cells assessed as cell survival after 72 hrs by MTT assay Anticancer activity against human MDA-MB-436 cells assessed as cell survival after 72 hrs by MTT assay	[PMID: 20137939]
THP-1	IC₅₀	5.3 3 Compound: Chlorambucil	Antiproliferative activity against human THP1 cells treated for 24 hrs measured after 72 hrs by MTT assay Antiproliferative activity against human THP1 cells treated for 24 hrs measured after 72 hrs by MTT assay	[PMID: 29037951]
HCT-15	IC₅₀	49700 1 Compound: Chl	Cytotoxicity against human HCT15 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human HCT15 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 30996795]
MDA-MB-468	IC₅₀	0.65 3 Compound: C5	Cytotoxicity against human MDA-MB-468 cells expressing estrogen receptor after 5 days by MTT assay Cytotoxicity against human MDA-MB-468 cells expressing estrogen receptor after 5 days by MTT assay	[PMID: 19121874]
BXPC-3	IC₅₀	75300 1 Compound: Chl	Cytotoxicity against human BxPC3 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human BxPC3 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 30996795]
MDA-MB-468	IC₅₀	0.79 3 Compound: C5	Cytotoxicity against human MDA-MB-468 cells transfected with NAD(P)H: quinone oxidoreductase NQO1 after 5 days by MTT assay Cytotoxicity against human MDA-MB-468 cells transfected with NAD(P)H: quinone oxidoreductase NQO1 after 5 days by MTT assay	[PMID: 19121874]
U-118-MG	IC₅₀	>40 3 Compound: CLB; 3d	Antiproliferative activity against human U-118-MG cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human U-118-MG cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36240546]
MDA-MB-468	IC₅₀	146.88 3 Compound: 3	Cytotoxicity against estrogen receptor-negative human MDA-MB-468 cells by MTT assay Cytotoxicity against estrogen receptor-negative human MDA-MB-468 cells by MTT assay	[PMID: 21051231]
U-251	IC₅₀	33.11 3 Compound: Chlorambucil	Anticancer activity against human U251 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Anticancer activity against human U251 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 32527536]
MDA-MB-468	IC₅₀	34.4 3 Compound: Chlorambucil	Anticancer activity against human MDA-MB-468 cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay Anticancer activity against human MDA-MB-468 cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay	[PMID: 28388522]
U-87MG ATCC	IC₅₀	18.82 6 Compound: Chlorambucil	Cytotoxicity against human U87 cells after 72 hrs by MTT assay Cytotoxicity against human U87 cells after 72 hrs by MTT assay	10.1007/s00044-013-0582-8
MDA-MB-468	IC₅₀	34.4 3 Compound: Chlorambucil	Growth inhibition of human MDA-MB-468 cells after 48 hrs by fluorescence polarisation assay Growth inhibition of human MDA-MB-468 cells after 48 hrs by fluorescence polarisation assay	[PMID: 30247905]
MDA-MB-468	IC₅₀	43.8 3 Compound: 1	Cytotoxicity against human MDA-MB-468 cells after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-468 cells after 72 hrs by MTT assay	[PMID: 24147900]
U-87MG ATCC	IC₅₀	18.8 3 Compound: Chlorambucil	Cytotoxicity against human U87 cells assessed as reduction in cell viability after 3 days by MTT assay Cytotoxicity against human U87 cells assessed as reduction in cell viability after 3 days by MTT assay	[PMID: 29649739]
MDA-MB-468	IC₅₀	67.5 3 Compound: 1	Cytotoxicity against human MDA-MB-468 cells after 72 hrs by MTT assay in presence of NAC Cytotoxicity against human MDA-MB-468 cells after 72 hrs by MTT assay in presence of NAC	[PMID: 24147900]
UO-31	IC₅₀	40.7 3 Compound: Chlorambucil	Anticancer activity against human UO-31 cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay Anticancer activity against human UO-31 cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay	[PMID: 28388522]
MDCK	IC₅₀	100 3 Compound: Chlorambucil	Cytotoxicity against carbonic anhydrase 9 knockdown MDCK cells assessed as reduction in cell viability after 2 hrs under hypoxic condition by Alamar blue assay Cytotoxicity against carbonic anhydrase 9 knockdown MDCK cells assessed as reduction in cell viability after 2 hrs under hypoxic condition by Alamar blue assay	[PMID: 27823879]
UV4	IC₅₀	1.2 3 Compound: 3	Growth inhibition activity was measured in repair deficient UV4 cells under aerobic conditions (4 hours drug exposure) Growth inhibition activity was measured in repair deficient UV4 cells under aerobic conditions (4 hours drug exposure)	[PMID: 7707327]
MDCK	IC₅₀	93 3 Compound: Chlorambucil	Cytotoxicity against carbonic anhydrase 9 knockdown MDCK cells assessed as reduction in cell viability preincubated for 2 hrs followed by 72 hrs incubation under normoxic condition by Alamar blue assay Cytotoxicity against carbonic anhydrase 9 knockdown MDCK cells assessed as reduction in cell viability preincubated for 2 hrs followed by 72 hrs incubation under normoxic condition by Alamar blue assay	[PMID: 27823879]
UV4	IC₅₀	58 3 Compound: cholambucil	Hypersensitivity factor obtained from in vitro cytotoxicities against Chinese hamster ovary derived AA8 / UV4 cell lines Hypersensitivity factor obtained from in vitro cytotoxicities against Chinese hamster ovary derived AA8 / UV4 cell lines	[PMID: 1992124]
MDCK	IC₅₀	> 100 3 Compound: Chlorambucil	Cytotoxicity against MDCK cells expressing carbonic anhydrase 9 assessed as reduction in cell viability after 2 hrs under hypoxic condition by Alamar blue assay Cytotoxicity against MDCK cells expressing carbonic anhydrase 9 assessed as reduction in cell viability after 2 hrs under hypoxic condition by Alamar blue assay	[PMID: 27823879]
UV4	IC₅₀	0.6 3 Compound: 2 (chlorambucil)	In vitro cytotoxicity against transformed chinese hamster fibroblast line UV4. In vitro cytotoxicity against transformed chinese hamster fibroblast line UV4.	[PMID: 2319563]
WiDr	IC₅₀	7.13 3 Compound: C5	Cytotoxicity against human WiDr cells after 5 days by MTT assay Cytotoxicity against human WiDr cells after 5 days by MTT assay	[PMID: 19121874]
MDCK	IC₅₀	> 100 3 Compound: Chlorambucil	Cytotoxicity against MDCK cells expressing carbonic anhydrase 9 assessed as reduction in cell viability preincubated for 2 hrs followed by 72 hrs incubation under normoxic condition by Alamar blue assay Cytotoxicity against MDCK cells expressing carbonic anhydrase 9 assessed as reduction in cell viability preincubated for 2 hrs followed by 72 hrs incubation under normoxic condition by Alamar blue assay	[PMID: 27823879]
MGC-803	IC₅₀	133.64 3 Compound: chlorambucil	Antiproliferative activity against human MGC803 cells assessed as growth inhibition after 72 hrs by MTT assay Antiproliferative activity against human MGC803 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25050168]
WM 266-4	GI₅₀	>25 3 Compound: CLB	Growth inhibition of human WM266.4 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human WM266.4 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
MIA PaCa-2	CC₅₀	> 150 3 Compound: Chlorambucil	Cytotoxicity against human MIAPaCa2 cells assessed as decrease in cell viability after 48 hrs by MTS assay Cytotoxicity against human MIAPaCa2 cells assessed as decrease in cell viability after 48 hrs by MTS assay	[PMID: 29043812]
MIA PaCa-2	CC₅₀	> 150 3 Compound: Chlorambucil	Cytotoxicity against human MIA PaCa-2 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Cytotoxicity against human MIA PaCa-2 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 29126728]
MIA PaCa-2	IC₅₀	78.3 3 Compound: 1	Cytotoxicity against human MIAPaCa2 cells after 72 hrs by MTT assay Cytotoxicity against human MIAPaCa2 cells after 72 hrs by MTT assay	[PMID: 24147900]
MIA PaCa-2	IC₅₀	> 100 3 Compound: Chlorambucil	Antiproliferative activity against human MIAPaCa2 cells after 72 hrs by MTT assay Antiproliferative activity against human MIAPaCa2 cells after 72 hrs by MTT assay	[PMID: 28129978]
MOLT-4	IC₅₀	6.3 3 Compound: Chlorambucil	In vitro inhibitory activity against acute lymphoblastic leukemia (MOLT4) cell line. In vitro inhibitory activity against acute lymphoblastic leukemia (MOLT4) cell line.	[PMID: 9667961]
MV4-11	IC₅₀	8.55 3 Compound: Chlorambucil	Antiproliferative activity against human MV4-11 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human MV4-11 cells incubated for 72 hrs by MTT assay	[PMID: 31082763]
NCI-H1299	GI₅₀	4.9 3 Compound: 1	Growth inhibition of human p53 deficient H1299 cells after 7 days by celltiter-glo assay Growth inhibition of human p53 deficient H1299 cells after 7 days by celltiter-glo assay	[PMID: 20973561]
NCI-H1299	GI₅₀	> 25 3 Compound: CLB	Growth inhibition of human NCI-H1299 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human NCI-H1299 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
NCI-H1299	GI₅₀	> 25 3 Compound: CLB	Growth inhibition of human NCI-H1299 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human NCI-H1299 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
NCI-H1299	IC₅₀	116.3 3 Compound: Chlorambucil	Antiproliferative activity against human H1299 cells after 72 hrs CCK8 assay Antiproliferative activity against human H1299 cells after 72 hrs CCK8 assay	[PMID: 30108928]
NCI-H1975	IC₅₀	28.16 3 Compound: Chlorambucil	Growth inhibition of human NCI-H1975 cells incubated for 5 days by SRB assay Growth inhibition of human NCI-H1975 cells incubated for 5 days by SRB assay	[PMID: 32145644]
NCI-H460	CC₅₀	104 3 Compound: Chlorambucil	Cytotoxicity against human H460 cells after 24 hrs by MTT assay Cytotoxicity against human H460 cells after 24 hrs by MTT assay	[PMID: 20615582]
NCI-H460	GI₅₀	11 3 Compound: 1	Growth inhibition of human NCI-H460 cells after 7 days by celltiter-glo assay Growth inhibition of human NCI-H460 cells after 7 days by celltiter-glo assay	[PMID: 20973561]
NCI-H460	IC₅₀	2.19 3 Compound: C5	Cytotoxicity against human H460 cells after 5 days by MTT assay Cytotoxicity against human H460 cells after 5 days by MTT assay	[PMID: 19121874]
NCI-H460	IC₅₀	28.9 3 Compound: Chlorambucil	Antiproliferative activity against human H460 cells after 72 hrs CCK8 assay Antiproliferative activity against human H460 cells after 72 hrs CCK8 assay	[PMID: 30108928]
NCI/ADR-RES	GI₅₀	56 3 Compound: Chlorambucil	Tested for in vitro concentration required to inhibit growth by 50% against MCF-7/ADR human breast cancer cell line cell line Tested for in vitro concentration required to inhibit growth by 50% against MCF-7/ADR human breast cancer cell line cell line	[PMID: 11755363]
NIH3T3	IC₅₀	> 600 3 Compound: CLB	Cytotoxicity against mouse NIH/3T3 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 28818459]
P388	IC₅₀	225 3 Compound: cholambucil	Optimal dose administered as a single dose by intraperitoneal inoculation of 10 X E6 murine leukemia P388 cell lines. Optimal dose administered as a single dose by intraperitoneal inoculation of 10 X E6 murine leukemia P388 cell lines.	[PMID: 1992124]
P388	IC₅₀	37 3 Compound: cholambucil	Percentage increase in lifespan of drug-related tumor bearing animals when heated at the optimal dose and tested in murine leukemia P388 cell lines. Percentage increase in lifespan of drug-related tumor bearing animals when heated at the optimal dose and tested in murine leukemia P388 cell lines.	[PMID: 1992124]
P388	IC₅₀	6.75 3 Compound: 2 (chlorambucil)	Tested for the in vitro cytotoxicity against P388 murine leukemia Tested for the in vitro cytotoxicity against P388 murine leukemia	[PMID: 2319563]
P388	IC₅₀	6.75 3 Compound: Chlorambucil	Inhibition of cell growth in culture against wild type P388 murine leukemia Inhibition of cell growth in culture against wild type P388 murine leukemia	[PMID: 2033580]
P388	IC₅₀	6.8 3 Compound: cholambucil	In vitro cytotoxicity against murine leukemia P388 cell line. In vitro cytotoxicity against murine leukemia P388 cell line.	[PMID: 1992124]
P388	IC₅₀	7 3 Compound: Chlorambucil	Dose required to inhibit cell growth was determined against P388D1 cell line of murine lymphocytotic leukemia Dose required to inhibit cell growth was determined against P388D1 cell line of murine lymphocytotic leukemia	[PMID: 11262080]
P388	IC₅₀	7.5 3 Compound: Chlorambucil	Cytotoxicity against P388 leukemia cell line Cytotoxicity against P388 leukemia cell line	[PMID: 2231599]
PA-1	IC₅₀	2.8 3 Compound: 2, Chlorambucil, CLB	Cytotoxicity against human PA1 cells by resazurin reduction test Cytotoxicity against human PA1 cells by resazurin reduction test	[PMID: 18424156]
PA-1	IC₅₀	2.8 3 Compound: CLB	Cytotoxicity against human PA1 assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test Cytotoxicity against human PA1 assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test	[PMID: 28927903]
PBMC	IC₅₀	> 200 3 Compound: Chlorambucil	Antiproliferative activity against human PBMC treated for 24 hrs measured after 72 hrs by MTT assay Antiproliferative activity against human PBMC treated for 24 hrs measured after 72 hrs by MTT assay	[PMID: 29037951]
PC-3	CC₅₀	> 150 3 Compound: Chlorambucil	Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs by MTS assay Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs by MTS assay	[PMID: 29043812]
PC-3	CC₅₀	> 150 3 Compound: Chlorambucil	Cytotoxicity against human PC-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Cytotoxicity against human PC-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 29126728]
PC-3	GI₅₀	> 25 3 Compound: CLB	Growth inhibition of human PC3 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human PC3 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
PC-3	GI₅₀	> 25 3 Compound: CLB	Growth inhibition of human PC3 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human PC3 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
PC-3	IC₅₀	131.3 3 Compound: Chlorambucil	Cytotoxicity against androgen receptor deficient human PC3 cells after 72 hrs by MTT assay Cytotoxicity against androgen receptor deficient human PC3 cells after 72 hrs by MTT assay	[PMID: 23665800]
PC-3	IC₅₀	16.4 3 Compound: Chlorambucil	Anticancer activity against human PC3 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Anticancer activity against human PC3 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 32527536]
PC-3	IC₅₀	40.35 3 Compound: 12	Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay	[PMID: 29407983]
PC-3	IC₅₀	40.35 3 Compound: Chlorambucil	Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay	[PMID: 29524728]
PC-3	IC₅₀	42.05 3 Compound: Chlorambucil	Antiproliferative activity against human PC3 cells treated for 24 hrs measured after 72 hrs by MTT assay Antiproliferative activity against human PC3 cells treated for 24 hrs measured after 72 hrs by MTT assay	[PMID: 29037951]
PC-3	IC₅₀	> 100 3 Compound: CLB	Cytotoxicity against human PC3 assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test Cytotoxicity against human PC3 assessed as inhibition of cell growth incubated for 48 hrs by resazurin reduction test	[PMID: 28927903]
PC-9	IC₅₀	7.17 3 Compound: Chlorambucil	Growth inhibition of human PC-9 cells incubated for 5 days by SRB assay Growth inhibition of human PC-9 cells incubated for 5 days by SRB assay	[PMID: 32145644]
Panel NCI-60 (60 carcinoma cell lines)	GI₅₀	60 3 Compound: 53	Antiproliferative activity against human NCI60 cells after 48 hrs by SRB assay Antiproliferative activity against human NCI60 cells after 48 hrs by SRB assay	[PMID: 29870668]
Raji	IC₅₀	298 3 Compound: chlorambucil	In vitro cytotoxicity against Raji cells from human lymphoma In vitro cytotoxicity against Raji cells from human lymphoma	10.1016/0960-894X(94)80008-1
SF-268	GI₅₀	5.4 3 Compound: 1	Growth inhibition of human SF268 cells after 7 days by celltiter-glo assay Growth inhibition of human SF268 cells after 7 days by celltiter-glo assay	[PMID: 20973561]
SH-SY5Y	IC₅₀	> 40 3 Compound: CLB; 3d	Antiproliferative activity against human SH-SY5Y cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human SH-SY5Y cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36240546]
SK-BR-3	IC₅₀	247.5 3 Compound: 1	Cytotoxicity against human SKBR3 cells after 72 hrs by MTT assay Cytotoxicity against human SKBR3 cells after 72 hrs by MTT assay	[PMID: 24147900]
SK-LU-1	IC₅₀	300 3 Compound: Chlorambucil	Anticancer activity against human SKLU1 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Anticancer activity against human SKLU1 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 32527536]
SK-OV-3	GI₅₀	5.5 3 Compound: 1	Growth inhibition of human SKOV3 cells after 7 days by celltiter-glo assay Growth inhibition of human SKOV3 cells after 7 days by celltiter-glo assay	[PMID: 20973561]
SK-OV-3	IC₅₀	35 3 Compound: chlorambucil	Growth inhibition against SKOV3 wild-type ovarian cell lines Growth inhibition against SKOV3 wild-type ovarian cell lines	[PMID: 15027879]
SMMC-7721	IC₅₀	163 3 Compound: Chlorambucil	Antiproliferative activity against human SMMC7721 cells after 72 hrs CCK8 assay Antiproliferative activity against human SMMC7721 cells after 72 hrs CCK8 assay	[PMID: 30108928]
SN12C	IC₅₀	135 3 Compound: Chlorambucil	Anticancer activity against human SN12C cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay Anticancer activity against human SN12C cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay	[PMID: 28388522]
THP-1	IC₅₀	5.3 3 Compound: Chlorambucil	Antiproliferative activity against human THP1 cells treated for 24 hrs measured after 72 hrs by MTT assay Antiproliferative activity against human THP1 cells treated for 24 hrs measured after 72 hrs by MTT assay	[PMID: 29037951]
U-118-MG	IC₅₀	> 40 3 Compound: CLB; 3d	Antiproliferative activity against human U-118-MG cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human U-118-MG cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36240546]
U-251	IC₅₀	33.11 3 Compound: Chlorambucil	Anticancer activity against human U251 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Anticancer activity against human U251 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 32527536]
U-87MG ATCC	IC₅₀	18.8 3 Compound: Chlorambucil	Cytotoxicity against human U87 cells assessed as reduction in cell viability after 3 days by MTT assay Cytotoxicity against human U87 cells assessed as reduction in cell viability after 3 days by MTT assay	[PMID: 29649739]
U-87MG ATCC	IC₅₀	18.82 6 Compound: Chlorambucil	Cytotoxicity against human U87 cells after 72 hrs by MTT assay Cytotoxicity against human U87 cells after 72 hrs by MTT assay	10.1007/s00044-013-0582-8
U-937	IC₅₀	37.7 3 Compound: Chlorambucil	Antiproliferative activity against human U-937 cells assessed as cell growth inhibition by MTT assay Antiproliferative activity against human U-937 cells assessed as cell growth inhibition by MTT assay	[PMID: 37875056]
UO-31	IC₅₀	40.7 3 Compound: Chlorambucil	Anticancer activity against human UO-31 cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay Anticancer activity against human UO-31 cells assessed inhibition of cell growth after 48 hrs by CellTiter-Glo luminescent assay	[PMID: 28388522]
UV4	IC₅₀	0.6 3 Compound: 2 (chlorambucil)	In vitro cytotoxicity against transformed chinese hamster fibroblast line UV4. In vitro cytotoxicity against transformed chinese hamster fibroblast line UV4.	[PMID: 2319563]
UV4	IC₅₀	1.2 3 Compound: 3	Growth inhibition activity was measured in repair deficient UV4 cells under aerobic conditions (4 hours drug exposure) Growth inhibition activity was measured in repair deficient UV4 cells under aerobic conditions (4 hours drug exposure)	[PMID: 7707327]
UV4	IC₅₀	58 3 Compound: cholambucil	Hypersensitivity factor obtained from in vitro cytotoxicities against Chinese hamster ovary derived AA8 / UV4 cell lines Hypersensitivity factor obtained from in vitro cytotoxicities against Chinese hamster ovary derived AA8 / UV4 cell lines	[PMID: 1992124]
WM 266-4	GI₅₀	> 25 3 Compound: CLB	Growth inhibition of human WM266.4 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human WM266.4 cells preincubated for 6 hrs cotreated with amonafide followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
WM 266-4	GI₅₀	> 25 3 Compound: CLB	Growth inhibition of human WM266.4 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human WM266.4 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
WiDr	IC₅₀	7.13 3 Compound: C5	Cytotoxicity against human WiDr cells after 5 days by MTT assay Cytotoxicity against human WiDr cells after 5 days by MTT assay	[PMID: 19121874]

In Vitro

Chlorambucil can deprive the function of complementary strands of DNA molecules via alkalization-induced cross interaction, and then inhibits tumor cell proliferation. Chlorambucil (0, 2.5, 5, 10 μM) exhibits slight inhibitory effect on Raji cell apoptosis, but potently increases DR4 and DR5 mRNA expression in Raji cells. Chlorambucil (10 μM) in combination with Tumor necrosis factor (TNF) related apoptosis inducing ligand (TRAIL, 80 ng/ml) has synergistic effect on Raji cell apoptosis and inhibition on proliferation^[1].
Chlorambucil is a DNA alkylator at high doses and, at lower doses, acts as an inhibitor of synthesis of nuclear proteins, particularly histones. Increasing doses are associated with a higher frequency of apoptosis, whereas long-term maintenance therapy has been associated with mutations of the p53 gene, leading to secondary malignancies^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Chlorambucil (0.2 mg/kg, p.o.) in combination with levamisole (5 mg/kg) has enhanced anti-cancer effect on Ehrlich ascites carcinoma which elevates apoptosis of Ehrlich ascites carcinoma and the survival rate of the mice. However, Chlorambucil exhibits adverse effects on the liver and kidneys of mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Essai clinique

Masse moléculaire

304.21

Formule

C₁₄H₁₉Cl₂NO₂

CAS No.

305-03-3

Appearance

Solid

Color

White to yellow

SMILES

O=C(O)CCCC1=CC=C(N(CCCl)CCCl)C=C1

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvant et solubilité

In Vitro:

DMSO : ≥ 100 mg/mL (328.72 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2872 mL	16.4360 mL	32.8720 mL
5 mM	0.6574 mL	3.2872 mL	6.5744 mL
10 mM	0.3287 mL	1.6436 mL	3.2872 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité
Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureté et documentation

Purity: 99.84%

Select Batch:

Fiche technique (279 KB) SDS (644 KB)

COA (247 KB) LCMS (119 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Guo JX, et al. Synergistic effects of chlorambucil and TRAIL on apoptosis and proliferation of Raji cells. Eur Rev Med Pharmacol Sci. 2017 Oct;21(20):4703-4710. [Content Brief]

[2]. Salem FS, et al. Biochemical and pathological studies on the effects of levamisole and chlorambucil on Ehrlich ascites carcinoma-bearing mice. Vet Ital. 2011 Jan-Mar;47(1):89-95. [Content Brief]

[3]. Mohamed D, et al. Chlorambucil-adducts in DNA analyzed at the oligonucleotide level using HPLC-ESI MS. Chem Res Toxicol. 2009;22(8):1435-1446. [Content Brief]

[4]. Birnbaum AD, et al. Chlorambucil and malignancy. Ophthalmology. 2010;117(7):1466-1466.e1. [Content Brief]

Test cellulaire ^[1]	Cultured cells at log-growth phase are digested by trypsin into single cell suspension and are seeded into 96-well plate at 1000 per well density. The plate is placed in a 37°C chamber with 5% CO₂. After attached growth for 24 h, cells are treated with TRAIL at 0, 20, 40 and 80 ng/mL or Chlorambucil at 0, 2.5, 5 and 10 μM for 48 h. 10 μL CCK-8 reagent is added to each well, followed by incubation at 37°C for 4 h. Absorbance values at 450 nm are then measured by a micro-plate reader. Six parallel samples are performed in each treatment group. Cell proliferation rate (%) = mean value of experimental group/mean value of control group × 100%^[1]. MCE n'a pas confirmé de manière indépendante l'exactitude de ces méthodes. Ils sont pour référence seulement.
Administration animale ^[2]	Mice^[2] Female Swiss mice are divided randomly into five group (20 mice per group). Group 1 is kept as the control group, Group 2 receives intraperitoneal injection of by 2.5 × 10⁶ Ehrlich ascites carcinoma cell, Gropu 3 is treated orally with Chlorambucil 0.2 mg/kg body weight, Group 4 is treated orally with levamisole (5 mg/kg body weight) and Group 5 is treated orally with a combination of Chlorambucil and levamisole each day, using a bent stainless steel stomach tube^[2]. MCE n'a pas confirmé de manière indépendante l'exactitude de ces méthodes. Ils sont pour référence seulement.
Références	[1]. Guo JX, et al. Synergistic effects of chlorambucil and TRAIL on apoptosis and proliferation of Raji cells. Eur Rev Med Pharmacol Sci. 2017 Oct;21(20):4703-4710. [Content Brief] [2]. Salem FS, et al. Biochemical and pathological studies on the effects of levamisole and chlorambucil on Ehrlich ascites carcinoma-bearing mice. Vet Ital. 2011 Jan-Mar;47(1):89-95. [Content Brief] [3]. Mohamed D, et al. Chlorambucil-adducts in DNA analyzed at the oligonucleotide level using HPLC-ESI MS. Chem Res Toxicol. 2009;22(8):1435-1446. [Content Brief] [4]. Birnbaum AD, et al. Chlorambucil and malignancy. Ophthalmology. 2010;117(7):1466-1466.e1. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2872 mL	16.4360 mL	32.8720 mL	82.1801 mL
	5 mM	0.6574 mL	3.2872 mL	6.5744 mL	16.4360 mL
	10 mM	0.3287 mL	1.6436 mL	3.2872 mL	8.2180 mL
	15 mM	0.2191 mL	1.0957 mL	2.1915 mL	5.4787 mL
	20 mM	0.1644 mL	0.8218 mL	1.6436 mL	4.1090 mL
	25 mM	0.1315 mL	0.6574 mL	1.3149 mL	3.2872 mL
	30 mM	0.1096 mL	0.5479 mL	1.0957 mL	2.7393 mL
	40 mM	0.0822 mL	0.4109 mL	0.8218 mL	2.0545 mL
	50 mM	0.0657 mL	0.3287 mL	0.6574 mL	1.6436 mL
	60 mM	0.0548 mL	0.2739 mL	0.5479 mL	1.3697 mL
	80 mM	0.0411 mL	0.2055 mL	0.4109 mL	1.0273 mL
	100 mM	0.0329 mL	0.1644 mL	0.3287 mL	0.8218 mL

Chlorambucil Related Classifications

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Chlorambucil (Synonyms: CB-1348; WR-139013)

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