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  3. Hesperidin

Hesperidin  (Synonyms: Hesperetin 7-rutinoside)

Cat. No.: HY-15337 Purity: 98.06%
COA Handling Instructions

Hesperidin (Hesperetin 7-rutinoside), a flavanone glycoside, is isolated from citrus fruits. Hesperidin has numerous biological properties, such as decreasing inflammatory mediators and exerting significant antioxidant effects. Hesperidin also exhibits antitumor and antiallergic activities.

For research use only. We do not sell to patients.

Hesperidin Chemical Structure

Hesperidin Chemical Structure

CAS No. : 520-26-3

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Solid + Solvent
10 mM * 1 mL in DMSO
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10 mg USD 66 In-stock
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500 mg USD 251 In-stock
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Customer Review

Based on 12 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Hesperidin purchased from MedChemExpress. Usage Cited in: Cell Rep. 2017 Nov 21;21(8):2147-2159.  [Abstract]

    U2OS cells expressing Myc-tagged PLK1 WT, K492R, or SUMO-1-K492R are treated with NSC 125973 for 16 hr. Half of the cells are then released in the presence of Hesperidin for 45 min to enrich cells at the later stage of M phase. Cell lysates are subject to IP, followed by western blot.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Hesperidin (Hesperetin 7-rutinoside), a flavanone glycoside, is isolated from citrus fruits. Hesperidin has numerous biological properties, such as decreasing inflammatory mediators and exerting significant antioxidant effects. Hesperidin also exhibits antitumor and antiallergic activities[1][2].

    In Vitro

    Hesperidin (5-200 μM; 24-72 h) induces potent cytotoxic effects in human osteosarcoma MG-63 cells[1].
    Hesperidin (5-150 μM; 48 h) induces early and late apoptosis in MG-63 cells[1].
    Hesperidin (10-30 μM) inhibits the activity of COX-2 and iNOS in a dose dependent manner in RAW 264.7 cells activated with LPS[2].
    Hesperidin (0.1 μg/mL; 2 h) decreases the formation of MDA and intracellular ROS, including chondrocyte apoptosis[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: MG-63 cells
    Concentration: 0, 5, 25, 50, 100, 150, 200 µM
    Incubation Time: 24, 48, 72 hours
    Result: Led to time-dependent and concentration-dependent cytotoxic effects, with IC50s of 94.3, 78.6 and 63.3 µM at 24, 48 and 72 h, respectively.

    Apoptosis Analysis[1]

    Cell Line: MG-63 cells
    Concentration: 0, 5, 50, 150 µM
    Incubation Time: 48 hours
    Result: Increased the percentage of apoptotic cells from 4.7% to 17.9, 34.6 and 68.3% at the concentration of 0, 5, 50 and 150 µM, respectively.
    In Vivo

    Hesperidin (5-80 mg/kg; 2 weeks) significantly suppresses MG-63 tumor growth in mice[1].
    Hesperidin (200 mg/kg; once daily for 28 d) markedly attenuates cartilage destruction and reduces IL-1β and TNF-α levels in a surgically-induced osteoarthritis (OA) rats[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Clinical Trial
    Molecular Weight

    610.56

    Formula

    C28H34O15

    CAS No.
    Appearance

    Solid

    Color

    Light yellow to khaki

    SMILES

    OC1=C(OC)C=CC([C@@H]2CC(C3=C(C=C(O[C@@H]4O[C@H](CO[C@@H]5O[C@@H](C)[C@H](O)[C@@H](O)[C@H]5O)[C@@H](O)[C@H](O)[C@H]4O)C=C3O)O2)=O)=C1

    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (163.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6378 mL 8.1892 mL 16.3784 mL
    5 mM 0.3276 mL 1.6378 mL 3.2757 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

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    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.6378 mL 8.1892 mL 16.3784 mL 40.9460 mL
    5 mM 0.3276 mL 1.6378 mL 3.2757 mL 8.1892 mL
    10 mM 0.1638 mL 0.8189 mL 1.6378 mL 4.0946 mL
    15 mM 0.1092 mL 0.5459 mL 1.0919 mL 2.7297 mL
    20 mM 0.0819 mL 0.4095 mL 0.8189 mL 2.0473 mL
    25 mM 0.0655 mL 0.3276 mL 0.6551 mL 1.6378 mL
    30 mM 0.0546 mL 0.2730 mL 0.5459 mL 1.3649 mL
    40 mM 0.0409 mL 0.2047 mL 0.4095 mL 1.0237 mL
    50 mM 0.0328 mL 0.1638 mL 0.3276 mL 0.8189 mL
    60 mM 0.0273 mL 0.1365 mL 0.2730 mL 0.6824 mL
    80 mM 0.0205 mL 0.1024 mL 0.2047 mL 0.5118 mL
    100 mM 0.0164 mL 0.0819 mL 0.1638 mL 0.4095 mL
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Hesperidin
    Cat. No.:
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