Cat. No.: HY-15337

Hesperidin

(Synonyms: Hesperetin 7-rutinoside)

30 Cited Publications

Customer Review

Based on 30 publication(s) in Google Scholar

Hesperidin (Hesperetin 7-rutinoside), a flavanone glycoside, is isolated from citrus fruits. Hesperidin has numerous biological properties, such as decreasing inflammatory mediators and exerting significant antioxidant effects. Hesperidin also exhibits antitumor and antiallergic activities.

For research use only. We do not sell to patients.

Purity: 99.14%
CAS No.: 520-26-3
Formula: C₂₈H₃₄O₁₅
Molecular Weight:610.56

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Hesperidin Chemical Structure

CAS No.: 520-26-3

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (30)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
3T3-L1	IC₅₀	311 μM Compound: 11	Anticorpulence activity against mouse 3T3L1 cells assessed as inhibition of lipid droplet accumulation Anticorpulence activity against mouse 3T3L1 cells assessed as inhibition of lipid droplet accumulation	[PMID: 19054677]
A549	IC₅₀	>100 μM Compound: 12	Cytotoxicity against human A549 cells after 48 hrs by resazurin reduction assay Cytotoxicity against human A549 cells after 48 hrs by resazurin reduction assay	[PMID: 20413315]
DLD-1	IC₅₀	>100 μM Compound: 12	Cytotoxicity against human DLD1 cells after 48 hrs by resazurin reduction assay Cytotoxicity against human DLD1 cells after 48 hrs by resazurin reduction assay	[PMID: 20413315]
H9	EC₅₀	>164 μM Compound: 21	Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay	[PMID: 8158164]
H9	IC₅₀	>164 μM Compound: 21	Cytotoxicity against human H9 cells after 3 days Cytotoxicity against human H9 cells after 3 days	[PMID: 8158164]
HT-29	IC₅₀	142 μM Compound: 11	Anticancer activity against human HT-29 cells after 72 hrs by MTT assay Anticancer activity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 19054677]
Monocyte	IC₅₀	>200 μM Compound: Hesperidin	Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA	[PMID: 10096854]

In Vitro

Hesperidin (5-200 μM; 24-72 h) induces potent cytotoxic effects in human osteosarcoma MG-63 cells^[1].
Hesperidin (5-150 μM; 48 h) induces early and late apoptosis in MG-63 cells^[1].
Hesperidin (10-30 μM) inhibits the activity of COX-2 and iNOS in a dose dependent manner in RAW 264.7 cells activated with LPS^[2].
Hesperidin (0.1 μg/mL; 2 h) decreases the formation of MDA and intracellular ROS, including chondrocyte apoptosis^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

MG-63 cells
Concentration:

0, 5, 25, 50, 100, 150, 200 µM
Incubation Time:

24, 48, 72 hours
Result:

Led to time-dependent and concentration-dependent cytotoxic effects, with IC₅₀s of 94.3, 78.6 and 63.3 µM at 24, 48 and 72 h, respectively.

Apoptosis Analysis^[1]

Cell Line:

MG-63 cells
Concentration:

0, 5, 50, 150 µM
Incubation Time:

48 hours
Result:

Increased the percentage of apoptotic cells from 4.7% to 17.9, 34.6 and 68.3% at the concentration of 0, 5, 50 and 150 µM, respectively.

In Vivo

Hesperidin (5-80 mg/kg; 2 weeks) significantly suppresses MG-63 tumor growth in mice^[1].
Hesperidin (200 mg/kg; once daily for 28 d) markedly attenuates cartilage destruction and reduces IL-1β and TNF-α levels in a surgically-induced osteoarthritis (OA) rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 520-26-3
Appearance Solid
Molecular Weight 610.56
Formula C₂₈H₃₄O₁₅
Color Light yellow to khaki
SMILES

OC1=C(OC)C=CC([C@@H]2CC(C3=C(C=C(O[C@@H]4O[C@H](CO[C@@H]5O[C@@H](C)[C@H](O)[C@@H](O)[C@H]5O)[C@@H](O)[C@H](O)[C@H]4O)C=C3O)O2)=O)=C1
Synonyms

Hesperetin 7-rutinoside
Structure Classification
- Flavonoids
- Flavonones
Initial Source
- Plants
- Rutaceae
- Citrus
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (30)

All In Vivo Efficacy StudyHistological Imaging/StainingRT-PCRWB

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Journal Impact Factor

Nat Commun

Integrative residue-intuitive machine learning and MD Approach to Unveil Allosteric Site and Mechanism for β2AR. [Abstract]

2024 Sep 16;15(1):8130. PMID: 39285201
Acta Pharm Sin B

Chrysin serves as a novel inhibitor of DGK α/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC). [Abstract]

2021 Jan;11(1):143-155. PMID: 33532186
Metabolism

WWP1-SHARP1-C/EBPβ positive feedback loop modulates development of metabolic dysfunction-associated steatotic liver disease. [Abstract]

2025 Apr 23:169:156271. PMID: 40280477

Hesperidin purchased from MedChemExpress. Usage Cited in: Metabolism. 2025 Apr 23:169:156271. [Abstract]

Eight–week–old NTG and OE–WWP1 C57 BL/6 J mice were intragastrically administered with the novel WWP1 inhibitor hesperidin (150 mg/kg body weight/day) and further fed an ND or HFHC for another 16 weeks. Average daily food intake (n = 6/group). Fecal TAG levels (n = 6/group). Liver organ index (n = 6/group). Energy expenditure (n = 6/group). Fasting blood glucose levels, fasting insulin levels, and HOMA–IR scores (n = 6/group).

Hesperidin purchased from MedChemExpress. Usage Cited in: Metabolism. 2025 Apr 23:169:156271. [Abstract]

Eight–week–old NTG and OE–WWP1 C57 BL/6 J mice were intragastrically administered with the novel WWP1 inhibitor hesperidin (150 mg/kg body weight/day) and further fed an ND or HFHC for another 16 weeks. Western blot analysis of AKT phosphorylation in mouse livers (n = 2/group).

Hesperidin purchased from MedChemExpress. Usage Cited in: Metabolism. 2025 Apr 23:169:156271. [Abstract]

Eight–week–old NTG and OE–WWP1 C57 BL/6 J mice were intragastrically administered with the novel WWP1 inhibitor hesperidin (150 mg/kg body weight/day) and further fed an ND or HFHC for another 16 weeks. H & E staining of mouse liver sections (n = 6/group). Oil Red O depicting hepatic lipid droplets; scale bar, 50 μm.

Hesperidin purchased from MedChemExpress. Usage Cited in: Metabolism. 2025 Apr 23:169:156271. [Abstract]

Eight–week–old NTG and OE–WWP1 C57 BL/6 J mice were intragastrically administered with the novel WWP1 inhibitor hesperidin (150 mg/kg body weight/day) and further fed an ND or HFHC for another 16 weeks. RT–qPCR analysis of the mRNA levels of genes involved in hepatic inflammation (n = 6/group).

Hesperidin purchased from MedChemExpress. Usage Cited in: Metabolism. 2025 Apr 23:169:156271. [Abstract]

Eight–week–old NTG and OE–WWP1 C57 BL/6 J mice were intragastrically administered with the novel WWP1 inhibitor hesperidin (150 mg/kg body weight/day) and further fed an ND or HFHC for another 16 weeks. Western blot detecting expressions of NF–κB pathways in livers (n = 2/group).
Cell Rep Med

High-fat diet increases circulating palmitic acid produced by gut Bacteroides thetaiotaomicron to promote thrombosis. [Abstract]

2025 Aug 19;6(8):102260. PMID: 40749683
Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530
Int J Biol Macromol

Hesperidin mitigates copper nanoparticle exposure-induced mitochondrial unfolded protein response through the SIRT3-FOXO3A signaling pathway. [Abstract]

2025 Jul 5;320(Pt 1):145750. PMID: 40617440
Phytomedicine

Fangchinoline suppresses nasopharyngeal carcinoma progression by inhibiting SQLE to regulate the PI3K/AKT pathway dysregulation. [Abstract]

2025 May:140:156484. PMID: 40090046
Cell Rep

SUMOylation Promotes Nuclear Import and Stabilization of Polo-like Kinase 1 to Support Its Mitotic Function. [Abstract]

2017 Nov 21;21(8):2147-2159. PMID: 29166606

Hesperidin purchased from MedChemExpress. Usage Cited in: Cell Rep. 2017 Nov 21;21(8):2147-2159. [Abstract]

U2OS cells expressing Myc-tagged PLK1 WT, K492R, or SUMO-1-K492R are treated with NSC 125973 for 16 hr. Half of the cells are then released in the presence of Hesperidin for 45 min to enrich cells at the later stage of M phase. Cell lysates are subject to IP, followed by western blot.
Phytother Res

Hesperidin Inhibits Oxidative Stress and Apoptosis of Granulosa Cells in Polycystic Ovarian Syndrome Through the JAK2/STAT3 and PI3K/AKT Pathways. [Abstract]

2025 Sep 27. PMID: 41014201
Ind Crops Prod

2025 Dec 11;239:122458.
Chin Med

Revealing potential anti-fibrotic mechanism of Ganxianfang formula based on RNA sequence. [Abstract]

2022 Feb 18;17(1):23. PMID: 35180857
ACS Biomater Sci Eng

Hesperidin-Loaded Nanoparticles Attenuate Pathological Angiogenesis in Oxygen-Induced Retinopathy by Modulating the Retinal Immune Microenvironment. [Abstract]

2026 Mar 9;12(3):1826-1847. PMID: 41741960
Drug Des Devel Ther

Si-Ni-San Reduces Hepatic Lipid Deposition in Rats with Metabolic Associated Fatty Liver Disease by AMPK/SIRT1 Pathway. [Abstract]

2023 Oct 3:17:3047-3060. PMID: 37808345
Nat Prod Bioprospect

Exploring anti-SARS-CoV-2 natural products: dual-viral target inhibition by delphinidin and the anti-coronaviral efficacy of deapio platycodin D. [Abstract]

2025 Jun 13;15(1):39. PMID: 40512442
Int J Mol Sci

Hesperetin from Root Extract of Clerodendrum petasites S. Moore Inhibits SARS-CoV-2 Spike Protein S1 Subunit-Induced NLRP3 Inflammasome in A549 Lung Cells via Modulation of the Akt/MAPK/AP-1 Pathway. [Abstract]

2022 Sep 7;23(18):10346. PMID: 36142258
Biomolecules

Structure-Based Virtual Screening and Functional Validation of Potential Hit Molecules Targeting the SARS-CoV-2 Main Protease. [Abstract]

2022 Nov 25;12(12):1754. PMID: 36551182
Plants

Determination of Flavonoid Glycoside Isomers Using Vision Transformer and Tandem Mass Spectrometry. [Abstract]

2024 Dec 4;13(23):3401. PMID: 39683194
Sci Rep

Hesperetin alleviates sepsis-associated acute respiratory distress syndrome via the PI3K/Akt/PTEN axis. [Abstract]

2026 Jan 3;16(1):3332. PMID: 41484432
Andrology

Therapeutic potential of hesperidin in diabetes mellitus-induced erectile dysfunction through Nrf2-mediated ferroptosis and oxidative stress. [Abstract]

2024 Nov 25. PMID: 39587831
J Neurosci Res

Enhanced mitochondrial inhibition by 3,4-dihydroxyphenyl-acetaldehyde (DOPAL)-oligomerized α-synuclein. [Abstract]

2019 Dec;97(12):1689-1705. PMID: 31420910
Chem Biol Drug Des

Potential Modulatory Effects of Hesperidin on the JAK/STAT Pathway in Mesangial Proliferative Glomerulonephritis. [Abstract]

2025 Feb;105(2):e70032. PMID: 39928525
Dis Model Mech

A Drosophila chemical screen reveals targeting MEK and DGKa mitigates Ras-driven polarity-impaired tumour growth. [Abstract]

2023 Mar 1;16(3):dmm049769. PMID: 36861754
J Cancer

Hesperidin inhibits colon cancer progression by downregulating SLC5A1 to suppress EGFR phosphorylation. [Abstract]

2025 Jan 1;16(3):876-887. PMID: 39781340
J Recept Signal Transduct Res

Function of hesperidin alleviating inflammation and oxidative stress responses in COPD mice might be related to SIRT1/PGC-1α/NF-κB signaling axis. [Abstract]

2020 Aug;40(4):388-394. PMID: 32164488
Biochem Biophys Res Commun

Hesperidin inhibits methylation and autophagy in LPS and high glucose-induced human villous trophoblasts. [Abstract]

2023 Sep 3:671:278-285. PMID: 37311265
Oncol Lett

Hesperidin inhibits tobacco smoke-induced pulmonary cell proliferation and EMT in mouse lung tissues via the p38 signaling pathway. [Abstract]

2022 Nov 29;25(1):30. PMID: 36589667
Future Sci OA

Hesperidin alleviates pulmonary fibrosis by regulating EI24-mediated autophagy. [Abstract]

2025 Dec;11(1):2483147. PMID: 40155367
SSRN

2025 Jul 8.
Ann Transl Med

ALKBH5 promotes the proliferation of renal cell carcinoma by regulating AURKB expression in an m6A-dependent manner. [Abstract]

2020 May;8(10):646. PMID: 32566583

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (163.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6378 mL

8.1892 mL

16.3784 mL

5 mM

0.3276 mL

1.6378 mL

3.2757 mL

10 mM

0.1638 mL

0.8189 mL

1.6378 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Du GY, et, al. Hesperidin exhibits in vitro and in vivo antitumor effects in human osteosarcoma MG-63 cells and xenograft mice models via inhibition of cell migration and invasion, cell cycle arrest and induction of mitochondrial-mediated apoptosis. Oncol Lett. 2018 Nov;16(5):6299-6306. [Content Brief]

[2]. Tejada S, et, al. Potential Anti-inflammatory Effects of Hesperidin from the Genus Citrus. Curr Med Chem. 2018;25(37):4929-4945. [Content Brief]

[3]. Gao G, et, al. Effects of Hesperidin on H₂O₂-Treated Chondrocytes and Cartilage in a Rat Osteoarthritis Model. Med Sci Monit. 2018 Dec 17;24:9177-9186. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.6378 mL	8.1892 mL	16.3784 mL	40.9460 mL
	5 mM	0.3276 mL	1.6378 mL	3.2757 mL	8.1892 mL
	10 mM	0.1638 mL	0.8189 mL	1.6378 mL	4.0946 mL
	15 mM	0.1092 mL	0.5459 mL	1.0919 mL	2.7297 mL
	20 mM	0.0819 mL	0.4095 mL	0.8189 mL	2.0473 mL
	25 mM	0.0655 mL	0.3276 mL	0.6551 mL	1.6378 mL
	30 mM	0.0546 mL	0.2730 mL	0.5459 mL	1.3649 mL
	40 mM	0.0409 mL	0.2047 mL	0.4095 mL	1.0237 mL
	50 mM	0.0328 mL	0.1638 mL	0.3276 mL	0.8189 mL
	60 mM	0.0273 mL	0.1365 mL	0.2730 mL	0.6824 mL
	80 mM	0.0205 mL	0.1024 mL	0.2047 mL	0.5118 mL
	100 mM	0.0164 mL	0.0819 mL	0.1638 mL	0.4095 mL

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Hesperidin

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Publications Citing Use of MedChemExpress (MCE) Hesperidin

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (30)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Hesperidin Related Classifications

Cat. No.: HY-15337