Ixabepilone (Synonyms: BMS-247550; Aza-epothilone B; イキサベピロン)

Name: Ixabepilone
Brand: MedChemExpress
SKU: HY-10222
Rating: 5.0 (9 reviews)

製品番号: HY-10222 純度: 99.84%: Data Sheet SDS COA 取扱説明書
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Ixabepilone (BMS-247550) is an orally bioavailable microtubule inhibitor, which binds to tubulin and promotes tubulin polymerization and microtubule stabilization, thereby arrests cells in the G2-M phase of the cell cycle and induces tumor cell apoptosis.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

Ixabepilone 構造式

CAS 番号 : 219989-84-1

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 279	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 279	在庫あり	Estimated Time of Arrival: December 31
Solid
1 mg	$119	在庫あり	Estimated Time of Arrival: December 31
5 mg	$250	在庫あり	Estimated Time of Arrival: December 31
10 mg	$440	在庫あり	Estimated Time of Arrival: December 31
50 mg		お問い合わせ
100 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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Based on 9 publication(s) in Google Scholar

Other Forms of Ixabepilone:

Ixabepilone (Standard) お問い合わせ

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•関連小分子:

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製品説明

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	2.6 1 Compound: Ixabepilone	Cytotoxicity against human HCT116 cells Cytotoxicity against human HCT116 cells	[PMID: 29251920]
1A9	IC₅₀	6.65 1 Compound: 12	Cytotoxicity against human 1A9 cells Cytotoxicity against human 1A9 cells	[PMID: 28850227]
CCRF-CEM	IC₅₀	3 1 Compound: Ixabepilone	Antiproliferative activity against human CCRF-CEM cells assessed as reduction in cell viability after 72 hrs by MTS assay Antiproliferative activity against human CCRF-CEM cells assessed as reduction in cell viability after 72 hrs by MTS assay	[PMID: 31419740]
CCRF-CEM	IC₅₀	3 1 Compound: Ixabepilone	Antiproliferative activity against human CCRF-CEM cells assessed as reduction in cell viability after 72 hrs by MTS assay Antiproliferative activity against human CCRF-CEM cells assessed as reduction in cell viability after 72 hrs by MTS assay	[PMID: 31419740]
KB	IC₅₀	8 1 Compound: Ixabepilone	Antiproliferative activity against human KB cells assessed as reduction in cell viability after 72 hrs by MTS assay Antiproliferative activity against human KB cells assessed as reduction in cell viability after 72 hrs by MTS assay	[PMID: 31419740]
1A9	IC₅₀	6.65 1 Compound: 12	Cytotoxicity against human 1A9 cells Cytotoxicity against human 1A9 cells	[PMID: 28850227]
KB	IC₅₀	8 1 Compound: Ixabepilone	Antiproliferative activity against human KB cells assessed as reduction in cell viability after 72 hrs by MTS assay Antiproliferative activity against human KB cells assessed as reduction in cell viability after 72 hrs by MTS assay	[PMID: 31419740]
CCRF-CEM	IC₅₀	3 1 Compound: Ixabepilone	Antiproliferative activity against human CCRF-CEM cells assessed as reduction in cell viability after 72 hrs by MTS assay Antiproliferative activity against human CCRF-CEM cells assessed as reduction in cell viability after 72 hrs by MTS assay	[PMID: 31419740]
HCT-116	IC₅₀	2.6 1 Compound: Ixabepilone	Cytotoxicity against human HCT116 cells Cytotoxicity against human HCT116 cells	[PMID: 29251920]
KB	IC₅₀	8 1 Compound: Ixabepilone	Antiproliferative activity against human KB cells assessed as reduction in cell viability after 72 hrs by MTS assay Antiproliferative activity against human KB cells assessed as reduction in cell viability after 72 hrs by MTS assay	[PMID: 31419740]

体外実験

BMS-247550 is a highly potent cytotoxic agent capable of killing cancer cells at low nanomolar concentrations and retains its antineoplastic activity against human cancers that are naturally insensitive to paclitaxel or that have developed resistance to paclitaxel^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

BMS-247550 demonstrates antitumor activity that is superior to paclitaxel in both paclitaxel-resistant and -sensitive tumors. BMS-247550 is more efficacious than paclitaxel in all five paclitaxel-resistant tumors evaluated in this study (four human and one murine): the clinically derived paclitaxel resistant Pat-7 ovarian carcinoma, the A2780Tax ovarian carcinoma that is resistant to paclitaxel because of tubulin mutations, the HCT116/VM46 MDR colon carcinoma, the clinically derived paclitaxel-resistant Pat-21 breast carcinoma, and the murine fibrosarcoma M5076. Against three paclitaxel-sensitive human tumor xenografts, BMS-247550 produces antitumor activity equivalent to paclitaxel: A2780 human ovarian carcinoma, HCT116, and LS174T human colon carcinoma^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

臨床実験

分子量

506.70

分子式

C₂₇H₄₂N₂O₅S

CAS 番号

219989-84-1

Appearance

Solid

Color

White to off-white

SMILES

O=C([C@@H]([C@H]([C@H](CCC[C@]1(O[C@]1(C[C@H](N2)/C(C)=C/C3=CSC(C)=N3)[H])C)C)O)C)C(C)([C@H](CC2=O)O)C

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶剤 & 溶解度

体外:

DMSO : 83.33 mg/mL (164.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9736 mL	9.8678 mL	19.7355 mL
5 mM	0.3947 mL	1.9736 mL	3.9471 mL
10 mM	0.1974 mL	0.9868 mL	1.9736 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.93%

Select Batch:

データシート (281 KB) SDS (393 KB)

COA (259 KB) HNMR (300 KB) RP-HPLC (135 KB) MS (71 KB)

取扱説明書 (2659 KB)

参考文献

[1]. John T. Hunt Discovery of Ixabepilone. Mol Cancer Ther February 2009 8; 275

キナーゼ実験 ^[1]	The potency with which BMS-247550 and paclitaxel polymerize tubulin isolated from calf brain is evaluated by Published techniques. Briefly, different concentrations of paclitaxel or BMS-247550 in polymerization buffer [0.1 M mes, 1 mM EGTA, 0.5 mM MgCl₂ (pH 6.6)] are added to tubulin in polymerization buffer at 37°C in microcuvette wells of a Beckman. Model DU 7400 UV spectrophotometer. A final microtubule protein concentration of 1.0 mg/mL and compound concentrations of generally 2.5, 5.0, and 10 μM are used. Initial slopes of absorbance (A280 nM) change, measured every 10 s, are calculated by the software program accompanying the instrument. MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。
細胞実験 ^[1]	HCT116 cells from cultures are collected by trypsinization after 1, 2, 4, 8, 16, and 24 h exposure to 7.5 nm of BMS-247550. Cells are pelleted and fixed in 80% ethanol at −20°C. After an overnight storage at −20°C, cells are rehydrated with PBS buffer and DNA stain by incubation with propidium iodide (5 μg/mL) in 0.1% RNase for 15-30 min. Fluorescence-activated cell sorter acquisition is performed using the FACS Calibur instrument and analysis is done using Cellquest and Modfit software. MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。
参考文献	[1]. John T. Hunt Discovery of Ixabepilone. Mol Cancer Ther February 2009 8; 275

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9736 mL	9.8678 mL	19.7355 mL	49.3389 mL
	5 mM	0.3947 mL	1.9736 mL	3.9471 mL	9.8678 mL
	10 mM	0.1974 mL	0.9868 mL	1.9736 mL	4.9339 mL
	15 mM	0.1316 mL	0.6579 mL	1.3157 mL	3.2893 mL
	20 mM	0.0987 mL	0.4934 mL	0.9868 mL	2.4669 mL
	25 mM	0.0789 mL	0.3947 mL	0.7894 mL	1.9736 mL
	30 mM	0.0658 mL	0.3289 mL	0.6579 mL	1.6446 mL
	40 mM	0.0493 mL	0.2467 mL	0.4934 mL	1.2335 mL
	50 mM	0.0395 mL	0.1974 mL	0.3947 mL	0.9868 mL
	60 mM	0.0329 mL	0.1645 mL	0.3289 mL	0.8223 mL
	80 mM	0.0247 mL	0.1233 mL	0.2467 mL	0.6167 mL
	100 mM	0.0197 mL	0.0987 mL	0.1974 mL	0.4934 mL

Ixabepilone Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ixabepilone219989-84-1BMS-247550 Aza-epothilone BBMS247550BMS 247550Microtubule/TubulinApoptosisBacterialInhibitorinhibitorinhibit

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Ixabepilone (Synonyms: BMS-247550; Aza-epothilone B; イキサベピロン)

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Other Forms of Ixabepilone:

Ixabepilone 関連抗体

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•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

生物活性

プロトコル

純度とドキュメンテーション

参考文献

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Ixabepilone 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Ixabepilone Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

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