2. Epigenetics Stem Cell/Wnt Protein Tyrosine Kinase/RTK JAK/STAT Signaling
  3. JAK
  4. JAK-IN-3

JAK-IN-3 (compound 22) is a potent JAK inhibitor, with IC50 values of 3 nM, 5 nM, 34 nM and 70 nM for JAK3, JAK1, TYK2 and JAK2, respectively.

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JAK-IN-3

JAK-IN-3 Chemische Struktur

CAS. Nr. : 1400876-94-9

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10 mM * 1 mL in DMSO
ready for reconstitution
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JAK-IN-3 Related Antibodies

Top Publications Citing Use of Products

Alle JAK Isoform-spezifische Produkte anzeigen:

Alle Isoformen anzeigen
JAK1 JAK2 JAK3 Tyk2 JAK

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Beschreibung

JAK-IN-3 (compound 22) is a potent JAK inhibitor, with IC50 values of 3 nM, 5 nM, 34 nM and 70 nM for JAK3, JAK1, TYK2 and JAK2, respectively[1].

IC50 & Target[1]

JAK3

3 nM (IC50)

JAK1

5 nM (IC50)

Tyk2

34 nM (IC50)

JAK2

70 nM (IC50)

Cellular Effect
Cell Line Type Value Description References
T-cell IC50
70 nM
Compound: 22
Inhibition of IL2-induced human PBMC-derived T cell proliferation preincubated for 60 mins followed by IL-2 stimulation and measured after 3 days by [3H]thymidine incorporation based topcount method
Inhibition of IL2-induced human PBMC-derived T cell proliferation preincubated for 60 mins followed by IL-2 stimulation and measured after 3 days by [3H]thymidine incorporation based topcount method
[PMID: 30891131]
Molekulargewicht

340.38

Formel

C18H20N4O3
CAS. Nr.
Appearance

Solid

Color

Light yellow to light brown

SMILES

O=C(C1=C(N[C@@H]([C@H](O)C)CO)C2=CC(C3=CC=CC=C3)=CN2N=C1)N
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro: 

DMSO : 62.5 mg/mL (183.62 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9379 mL 14.6895 mL 29.3789 mL
5 mM 0.5876 mL 2.9379 mL 5.8758 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (6.11 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation

Purity: 98.92%

SDS (251 KB)

COA (252 KB) HNMR (269 KB) LCMS (260 KB)

Handling Instructions (2659 KB)
Verweise

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9379 mL 14.6895 mL 29.3789 mL 73.4473 mL
5 mM 0.5876 mL 2.9379 mL 5.8758 mL 14.6895 mL
10 mM 0.2938 mL 1.4689 mL 2.9379 mL 7.3447 mL
15 mM 0.1959 mL 0.9793 mL 1.9586 mL 4.8965 mL
20 mM 0.1469 mL 0.7345 mL 1.4689 mL 3.6724 mL
25 mM 0.1175 mL 0.5876 mL 1.1752 mL 2.9379 mL
30 mM 0.0979 mL 0.4896 mL 0.9793 mL 2.4482 mL
40 mM 0.0734 mL 0.3672 mL 0.7345 mL 1.8362 mL
50 mM 0.0588 mL 0.2938 mL 0.5876 mL 1.4689 mL
60 mM 0.0490 mL 0.2448 mL 0.4896 mL 1.2241 mL
80 mM 0.0367 mL 0.1836 mL 0.3672 mL 0.9181 mL
100 mM 0.0294 mL 0.1469 mL 0.2938 mL 0.7345 mL
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JAK-IN-3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

JAK-IN-31400876-94-9JAKJanus kinaseInhibitorinhibitorinhibit

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